genetic instability and dna repair

... Donadi E: Efficiency of the DNA repair and polymorphisms of the XRCC1, XRCC3 and XRCC4 DNA repair genes in systemic lupus erythematosus Lupus 2008, 17:988-995 Fernandez D, Perl A: Metabolic control ... genetic susceptibility in SLE [20] Bassi and colleagues, however, reported that SLE leucocytes less efficiently repair radiation-induced DNA damage and that DNA repair gene polymorphic sites may predispose ... phosphorylation (ATP6, COX1, COX3, CYTB, ND1, and ND2), in which three are relevant to mitochondrial dysfunction Because the expressions of DNA repair and mtDNAencoded genes were found downregulated,...

Ngày tải lên: 12/08/2014, 15:23

... kinase-like protein kinases FAT FRAP, ATM, and TRRAP C-terminus Carboxy terminus dsDNA double-strand DNA ssDNA single-strand DNA SAP SAF-A/B, Acinus, and PIAS domain VHS Vps27p/Hrs/STAM XLF XRCC4-like ... to bind 50fmol of DNA Titration experiments were performed and demonstrate that these concentrations of Ku and DNA were in excess and DNA- PKcs is the limiting factor 2.8 DNA- PK DNA Binding/Recruitment ... interactions with Ku and DNA in vivo and in vitro66 In order to better understand how protein/protein and protein /DNA interactions influence DNA- PK activation, we created and purified Ku80 C-terminal...

Ngày tải lên: 24/08/2014, 09:33

... tail and 3’ blunt end, DnaB enters DNA with both strands passing through the central channel of DnaB (Kaplan, 2000) The translocation of DnaB on double-stranded DNA (dsDNA) requires ATP hydrolysis ... temporally and spatially separate DNA unwinding by DnaB helicase from its ATP-dependent locomotion on DNA (double- or single-stranded) It is known that when encountering a linear DNA with a 5’ tail and ... (Madabhushi and Marians, 2009) 10 Genetic Manipulation of DNA and Protein – Examples from Current Research Fig A substrate designed to separate temporally and spatially DnaB translocation from DNA unwinding...

Ngày tải lên: 06/03/2014, 23:20

... BER but also in DNA doublestrand break repair and meiosis, and Pol b and TdT may be evolved for differentiation and development of specific organs in vertebrates such as the nervous and immune systems ... meristem and panicle (lanes and in Fig 5A), and showed Plant DNA polymerase k (Eur J Biochem 271) 2805 moderate expression in the roots, root apices, flag leaves and young leaves (lanes 3, 4, and 7) ... involved in DNA repair and cell cycle checkpoint regulation on DNA damage [13] shows a lower degree of homology to human Pol k To determine the phylogenetic relationship between OsPol k and other...

Ngày tải lên: 07/03/2014, 15:20

... with orientations that differ between hHR23B and hHR23A are shown Also, b-N and b-C are the N- and C-termini of XPCB–hHR23B; a-N and a-C are the N- and C-termini of hHR23A FEBS Journal 272 (2005) ... (HT1 and HT2) were identified in XPCB–hHR23B, one between helix and helix 3, and one between helix and helix 4–5 These two trails were linked by a hydrophobic linker patch between helix and helix ... related to XPC and DNA repair [19] Here, we report the three-dimensional solution structure of the XPCB–hHR23B (275–342) fragment (XPCB–hHR23B), which contains the XPC interaction domain, and compare...

Ngày tải lên: 07/03/2014, 17:20

... involved in DNA repair and RNA metabolism in the protozoan parasites such as T gondii and the Plasmodia Further analyses should contribute to our understanding of the biochemistry of DNA repair proteins ... isolation and sequencing of the fulllength TgDRE cDNA The comparison of cDNA and genomic DNA nucleotide sequences allowed the determination of the exact position of the two introns which were 780- and ... P.C (1999) A phylogenomic study of DNA repair genes, proteins, and processes Mut Res 435, 171– 213 Marti, T.M., Kunz, C & Fleck, O (2002) DNA mismatch repair and mutation avoidance pathways J...

Ngày tải lên: 08/03/2014, 22:20

... tail and 3’ blunt end, DnaB enters DNA with both strands passing through the central channel of DnaB (Kaplan, 2000) The translocation of DnaB on double-stranded DNA (dsDNA) requires ATP hydrolysis ... temporally and spatially separate DNA unwinding by DnaB helicase from its ATP-dependent locomotion on DNA (double- or single-stranded) It is known that when encountering a linear DNA with a 5’ tail and ... (Madabhushi and Marians, 2009) 10 Genetic Manipulation of DNA and Protein – Examples from Current Research Fig A substrate designed to separate temporally and spatially DnaB translocation from DNA unwinding...

Ngày tải lên: 17/03/2014, 21:20

... lymphopenia and microcephaly Disrupted DNA repair pathway DSB repair, DNA damage signalling DSB repair, DNA damage signalling DSB repair, DNA damage signalling DSB repair DSB repair, DNA damage ... of DNA repair pathways 3.1 Base excision repair and single strand break repair Base excision repair (BER) is the major pathway responsible for handling the mutagenic and cytotoxic effects of DNA ... in response to DNA strand breaks J Cell Biol 173:195-206 Bekker-Jensen, S., and N Mailand 2010 Assembly and function of DNA double-strand break repair foci in mammalian cells DNA Repair (Amst)...

Ngày tải lên: 27/06/2014, 19:20

... lymphopenia and microcephaly Disrupted DNA repair pathway DSB repair, DNA damage signalling DSB repair, DNA damage signalling DSB repair, DNA damage signalling DSB repair DSB repair, DNA damage ... of DNA repair pathways 3.1 Base excision repair and single strand break repair Base excision repair (BER) is the major pathway responsible for handling the mutagenic and cytotoxic effects of DNA ... in response to DNA strand breaks J Cell Biol 173:195-206 Bekker-Jensen, S., and N Mailand 2010 Assembly and function of DNA double-strand break repair foci in mammalian cells DNA Repair (Amst)...

Ngày tải lên: 28/06/2014, 03:20

... 87(15):5837–5841 18 DNA Repair − On the Pathways to Fixing DNA Damage and Errors Holmes, AM & Haber, J (1999) Double-strand break repair in yeast requires both leading and lagging strand DNA polymerases, ... Sgs1 helicase and two nucleases Dna2 and Exo1 resect DNA double-strand break ends, Cell 134(6):981-994 2 Synergy Between DNA Replication and Repair Mechanisms Maria Zannis-Hadjopoulos and Emmanouil ... essential role in several DNA repair pathways, such as base excision repair, nucleotide excision repair, and double-strand (ds) break and mismatch repair Recent work from prokaryotes and eukaryotes has...

Ngày tải lên: 28/06/2014, 05:20

... means that DNA breakage and repair occurs in a non-physiological environment Whitaker and McMillan reported in 1992 that encapsulating cells before irradiation impair the estimation of DNA repair ... estimate DNA repair Nevertheless, the underlying mechanisms affecting cells embedded in agarose during DNA repair in PFGE methodology are still not well understood To better understand spontaneous DNA ... the study and drafted the manuscript GP, LL and MB participated in PFGE and in immunofluorescence studies XS provided informatics and support with statistics for data analysis SM, OC, RM and GC...

Ngày tải lên: 09/08/2014, 09:20

... understanding of the DNA double-strand break repair machinery of plants DNA Repair 2006, 5(1):1 Friesner J, Britt AB: Ku80- and DNA ligase IV-deficient plants are sensitive to ionizing radiation and ... the repair kinetics of single and double strand DNA breaks induced in wild type and silenced lines Of the different forms of DNA damage, DSBs are one of the most cytotoxic and, if left unrepaired, ... is required for joining DNA fragments produced during DNA replication DNA ligase also plays important roles in DNA single strand break (SSB) repair pathways in mammals and yeast These pathways...

Ngày tải lên: 12/08/2014, 03:20

... sequence-independent DNA binding activity, favoring double-stranded DNA over single-stranded DNA I demonstrated that the two proteins form a 1:1 stochiometric complex, and once formed, Metnase can localize to DNA ... cells, DNA double-strand breaks induced by IR and V(D)J recombination are mainly repaired by nonhomologous end-joining (NHEJ) (1-5) The NHEJ repair proteins Ku70/80, DNA PKcs, Artemis, and Xrcc4/ligase ... urea) for DNA cleavage DNA size makers indicated on the left B) Kinetic analysis of Metnase’s DNA cleavage activity with partial duplex DNA (3’- and 5’-overhangs) M1 and M2 represent two DNA markers...

Ngày tải lên: 24/08/2014, 10:50

... (NHEJ) DNA Repair Pathway A DSB occurs and the pathway is initiated by the Ku protein binding damaged DNA termini, translocates inward, and recruits DNA- PKcs to form the DNA- PK heterotrimer DNA- PK ... OF REDOX EFFECTS AND THE KU80 CTERMINAL REGION IN THE REGULATION OF THE HUMAN DNA REPAIR PROTEIN KU DNA double strand breaks (DSB) are among the most lethal forms of DNA damage and can occur as ... instability and contribute to the development and progression of cancer An increasing amount of research is drawing a close correlation between DNA repair and how it affects the development and treatment...

Ngày tải lên: 24/08/2014, 11:02

... polymorphisms on the risk of relapse In conclusion, DNA chip platform is a high-throughput and reliable technology for genetic diagnosis and pharmacogenetic profiling ix LIST OF TABLES Page Table ... Paediatrics, NUS, and Dr Heng Chew Kiat, Department of Paediatrics, NUS, for their guidance and expert advice which enabled me to perform my research systematically and their support in the review and revision ... design a rapid and robust methodology to simultaneously screen multiple mutations causing β-thalassemias We integrated the outstanding multiplexing capacity of DNA chip technology and high accuracy...

Ngày tải lên: 12/09/2015, 08:18

... randomly selected from M-c and M-e and assigned to OPT3 to replace M-a It is then found that M-a is also the current machine used by OPT1, OPT4, OPT5, and OPT2 Among these OPTs, OPT1, OPT4, and ... information (machines, tools, and all the cost indices) is described earlier and the required OPTs and their machine, tool, and TAD alternatives are shown in Table 9.2 and the PRs between the OPTs ... according to the following rule: IF AND AND THE F(X) → F(Y) OPT(i) ∈ F(X) OPT(j) ∈ F(Y) OPT(i) → OPT(j) As a result, both hard and soft PRs are identified The hard and soft PRs are compared pair-wise...

Ngày tải lên: 25/12/2013, 19:15

... general purpose and intended for the maintenance, repair and modification welding of molds, tools and dies Any tools in need of repair that could threaten life or limb if not repaired correctly, ... scratching, guessing and eventually going back to your source for the repair information again A customer may come back to you and tell you that the repair you made the last time was perfect and can he ... insulating material d Visual d Air d Fig 2.2 12 Handbook of mold, tool and die repair welding Pre-inspection: visual and dye pen A visual pre-inspection is standard on all tools All tools should be inspected...

Ngày tải lên: 05/01/2014, 19:45

... DNA- cleaving and RNA-cleaving DNAzyme (DRc DNAzyme) We elected to use an in vitro-selected pistol-like (PL) DNAzyme as a DNA scaffold for designing a DNAzyme with dual catalytic activities The PL DNAzyme ... FEBS D Jiang et al An RNA-cleaving and DNA- cleaving DNAzyme A B C D Fig The DNA- cleaving activity and cleavage sites of DRc DNAzyme (A) 5¢-32P-labeled DRc DNAzyme was incubated in buffer A at ... 8–17 variant DNAzyme The stem and terminal loop (5¢-AGC-3¢) of 8–17 variant DNAzyme replaces stem–loop I of the PL DNAzyme scaffold, and the T deoxyribonucleotide of 8–17 variant DNAzyme is inserted...

Ngày tải lên: 16/02/2014, 15:20

... An equimolar amount of complementary strand was added, and the mixture was heated for at 96 C and slowly cooled to room temperature The double-stranded DNA was stored at )20 C in experimental ... 1ị where SD and SRF are the respective areas under the emission spectra of the donor and a reference compound, and ARF and AD are the respective absorbances of the reference compound and donor ... 10PỵDAị=2 2ị where F and Fcor are uorescence intensity before and after the correction, and P and DA denote the initial sample absorbance at the excitation wavelength and the change in absorbance...

Ngày tải lên: 20/02/2014, 23:20

... Plasmids and Bacteriophages Pilus M13 particle injects DNA into cell (a) Injection of singlestranded DNA into the host cell, followed by synthesis of the second strand Single-stranded DNA Double-stranded ... pure DNA DNA Contaminants Pure DNA (b) Separation of DNA from contaminants + Pure DNA Contaminants Part I The Basic Principles of Gene Cloning and DNA Analysis 30 Figure 3.6 Aqueous layer (DNA ... Gene Cloning and DNA Analysis the experiments of Avery, MacLeod, and McCarty in 1944, and of Hershey and Chase in 1952, that anyone believed that deoxyribonucleic acid (DNA) is the genetic material:...

Ngày tải lên: 06/03/2014, 22:20