genetic alterations in normal and malignant breast tissue

... pathologists in an attempt to explain clinical palpability or mammographic density [4] But adenocarcinoma including ductal carcinoma, lobular carcinoma and in- situ ductal carcinoma studied in this ... Connective tissue that supports the malignant cells varies in composition from fibroblastic to densely hyaline and contains varying amounts of collagen, extra cellular mucin and elastic tissue [9] ... converted into photon energy by performing a calibration using Am-241 All samples were prepared as mm diameter and mm height pieces of tissue contained in low scattering, thin walled plastic holders In...

Ngày tải lên: 02/11/2012, 11:08

... estimation of proline and ornithine J Biol Chem 199, 91–95 45 Van Pilsum JF, Martin RP, Kito E & Hess J (1956) Determination of creatine, creatinine, arginine, guanidinoacetic acid, guanidine, and methyl-guanidine ... membrane occurs in malignant cells, and needs further investigation The presence of both AGAT and GAMT in EAC and S180 cells and in sarcoma tissue indicates that the upregulated proteins are entirely ... differences in ornithine and creatinine content in the blood of tumor-bearing mice compared to that in tumor-free mice, with the values of GAA differing significantly, being higher in tumor-bearing mice...

Ngày tải lên: 07/03/2014, 04:20

... higher level of TS in primary tumors than in normal tissues To study the significance of the differential expression of TS in primary tumors, clinicopathological features, including age, gender, ... Henan Provincial Cancer Hospital, Henan Provincial Institute of Cancer, Zhengzhou, Henan Province, China Department of Pathology, Henan Provincial Cancer Hospital, Henan Provincial Institute ... Zhengzhou, Henan Province, China Department of Surgery, Henan Provincial Cancer Hospital, Henan Provincial Institute of Cancer, Zhengzhou, Henan Province, China Authors’ contributions YL and SY designed...

Ngày tải lên: 10/08/2014, 21:23

... long chain fatty acids (Elovl3), type iodothyronine deiondinase (Dio2), homeobox C9 (Hoxc9), insulin-like growth factor binding protein (Igfbp3), dermatopontin (Dpt), and b-actin are showed in Table ... standard deviation inflammatory response and IR development, were examined in mice As shown in Figure 3, PM 2.5 exposure induced a marked increase in macrophage (F4/80+ cells) infiltration in ... reduced in both eWAT and iBAT in response to PM2.5 exposure Recent studies have implicated PM2.5 in increased adipose inflammation and insulin resistance [11,12], and epidemiological studies indicate...

Ngày tải lên: 06/03/2014, 19:20

... of exon 2, intron 1, exon and its 5¢-flanking noncoding sequence, and 2.7 kb of sequence containing the 3¢-part of exon and 3¢-flanking intron sequence, respectively These were inserted into a pBluescript ... J (1992) Low density lipoprotein receptor-related protein and gp330 bind similar ligands, including plasminogen activator-inhibitor complexes and lactoferrin, an inhibitor of chylomicron remnant ... cholesterol and TG that were carried in apo B48containing lipoproteins resembling remnants Nevertheless, in LDLR-deficient mice the increase in TG levels was much smaller than that obtained in RAP...

Ngày tải lên: 16/03/2014, 18:20

... non-silencing siRNA; and G.4 = MMP-3 siRNA main one forming connective tissue in all higher animals Connective tissue contains a mixture of cells, proteins, complex polysaccharides and inorganic ... was incubated at 42°C for 90 min, and terminated by heat inactivation at 70°C for 15 Measurement of GAG levels The level of GAG appearing in the medium of explants, cell cultures, and papain-digested ... proteoglycans, gelatin, laminin, fibronectin and collagen (types III, IV and IX) [10] In addition, MMP-3 can stimulate other enzymes in the MMP group, such as MMP-1, MMP7, MMP-8, MMP-9 and MMP-13 This...

Ngày tải lên: 20/06/2014, 04:20

... various factors influencing the radiolabeling process and in vitro stability of the labeled complex Subsequently blood kinetics in rabbits; and tissue distribution and gamma scintigraphy studies ... Radiolabeling and its subsequent quality control parameters, including radiochemical purity, in vitro and in vivo stability, blood kinetics and biodistribution studies were broadly determined as ... for ovarian tissue Further increased 99mTc-noscapine uptake seen in ovaries of PCOS induced animals, is Figure Whole body scintigraphic images of 99mTc-noscapine in control and PCOS induced female...

Ngày tải lên: 20/06/2014, 07:20

... pathogenetic processes Competing interests The authors declare that they have no competing interests Sulf-1 and Sulf-2 expression in murine temporomandibular joints joints Safranin O staining ... expression was also determined in murine knee joints Interestingly, Sulf expression was high at month of age and Figure Sulf-1 and Sulf-2 expression in specific zones of normal and osteoarthritis (OA) ... cartilage Sulf-1 and Sulf-2 protein expression in normal and osteoarthritis (OA) cartilage Sulf protein expression in normal (43 years old; Mankin score: 2) and OA (79 years old; Mankin score: 9)...

Ngày tải lên: 09/08/2014, 10:23

... expression in malignant carcinoma via TNF-R1 [25], and TNF-R1 but not TNF-R2 has been shown to be important in inflammatory responses involving IL-1β and MMP-3 and -9 in murine brain injury [26] ... 9:539-552 Alsalameh S, Amin R, Kunisch E, Jasin H, Kinne R: Preferential induction of prodestructive matrix metalloproteinase-1 and proinflammatory interleukin and prostaglandin E2 in rheumatoid arthritis ... types IV and V, and stromelysins (including MMP-3) that degrade predominantly non-collagenous proteins such as proteoglycans and fibronectin and can activate the pro-collagenases Having previously...

Ngày tải lên: 09/08/2014, 14:20

... Leghorn line and a Brown Egg line (11 DW dw males per line mated to or females each) maintained in our laboratory Hens were housed in individual cages in a single room under 14 h light and 10 h ... crossbreeding MATERIALS AND METHODS Birds Genetic types and genotypes have been described previously by M6rat et al (1994) Briefly, pure and crossbred, normal and dwarf progenies were obtained in a single ... g lighter in dwarf than in normal crossbred hens In the present study, the egg weight increase of normal and dwarf hens of parental pure lines (White Leghorn and Brown Egg) with age and their...

Ngày tải lên: 09/08/2014, 18:21

... separated into distinct clusters Next, we examined the divergence between Indian and non-Indian populations using pairwise FST estimates In comparing major continental groups, India and Europe have ... history of India has long intrigued anthropologists and human geneticists [3,12-14,20,34,35] A better understanding of Indian genetic diversity and population history can provide new insights into ... Humain collection in Utah, USA, with ancestry from Northern and Western Europe (CEU), Han Chinese in Beijing, China (CHB), Japanese in Tokyo, Japan (JPT), Yoruba in Ibadan, Nigeria (YRI), Chinese...

Ngày tải lên: 09/08/2014, 22:23

... points and examine if time point of reperfusion affected the result Data in every separate group passed normality test and differences between SEMs in compared groups (raw data) were due to random ... coronary interventions [19] Nevertheless, controversy still Figure Mean LVDP in various time points LVDP values in mmHg, time point in minutes Figure Mean of the product LVPDxHR in various time points ... preconditioned, normal or hypertrophic) couple of groups, in various time points The asterisk (*) shows significant differences LVDP and LVEDP in mmHg, time point in minutes In laboratory studies,...

Ngày tải lên: 10/08/2014, 09:21

... expressed in parthenocarpic fruit set, none of which were implicated in auxin biosynthesis One was involved in auxin transport, two in auxin signaling pathway, four were auxin induced proteins, five ... to auxin stimulus and one was a GH3-like protein involved in auxin and ethylene response (expression patterns in Table 5) We also investigated the function of ethylene in ovary development and ... measuring of endogenous levels in pollinated ovaries, in the unpollinated ovaries of parthenocarpic lines and by exogenous application [8] Several genes are also described as being involved in fruit...

Ngày tải lên: 12/08/2014, 03:20

... situ and in vitro the expression and localization of syncytin in human placenta at different stages of gestation, in normal and T21-affected pregnancies Results Syncytin is immunolocalized in cytotrophoblastic ... detected in the extravillous trophoblast (ECT), in the syncytiotrophoblast and in the mesenchymal core Scale bar = 10 µm D In this floating villi, syncytin immunostaining was observed in the cytoplasm ... impaired by abnormal hCG signaling in trisomy 21 pregnancies Endocrinology 2007 Frendo JL, Cronier L, Bertin G, Guibourdenche J, Vidaud M, EvainBrion D, Malassine A: Involvement of connexin 43 in human...

Ngày tải lên: 13/08/2014, 06:20

... retinol-binding protein-1 (CRBP-1) expression in normal 15 Cellularfetal liver Cellular retinol-binding protein-1 (CRBP-1) expression in normal fetal liver Around the sinusoid (S), CRBP1 stained ... was a weak staining of hepatocytes, principally in the youngest Figure retinol-binding protein-1 (CRBP-1) expression in normal 16 Cellularfetal liver Cellular retinol-binding protein-1 (CRBP-1) ... terminal venules, and centrolobular vein are stained (Figures 17, 18, 19 and 20) No portal mesenchymal cell, hepatocytic cell and sinusoidal cell were stained Figure retinol-binding protein-1...

Ngày tải lên: 13/08/2014, 13:20

... infection has to be mounted early and effectively, genetic influence on cytokine response in infection may determine effectiveness of bacterial killing [28] Supporting our results, it has been recently ... subsequent signaling is crucial for the innate immune system to initiate a response Genetic alterations affect this response and are related to individual variations in the course of sepsis In summary, ... higher risk of developing severe infectious complications following surgery as shown in two large studies including a total of 790 patients • Individuals carrying these two genetic variations had...

Ngày tải lên: 13/08/2014, 20:22

... heterochromatin protein (HP1), which recruits additional HP1 molecules and other chromatin-modifying proteins, thereby spreading and maintaining the heterochromatin state Since then, the involvement ... including the homeodomain, zinc-finger domain, leucine zipper domain, winged helix, erythroblast transformation-specific domain (ETS) and helix–loop–helix domains In the past, the determination ... DNA-binding domain Transcription factors are modular with generally distinct DNA-binding and transcriptional effector Oncogene domains A number of DNA-binding domains have been characterized including...

Ngày tải lên: 05/09/2015, 11:25

... effective in treating and preventing neurotoxin-induced Parkinson’s disease in rat via anti-oxidative stress and metabolic inhibition, highlighting the therapeutic potential of H2S in neurodegenerative ... Nitric oxide PAG DL-propargylglycine PCI Percutaneous coronary intervention PI3K Phosphatidylinositol 3-kinase PKA Protein kinase A PKC Protein kinase C PKG Protein Kinase G PLB Phosopholamban PLC ... (Simmerman and Jones, 1998) Troponin I is an inhibitory protein associated with troponin C and T complex on tropomyosin (Bers, 2002) Enhanced phosphorylation of troponin I decreases troponin C affinity...

Ngày tải lên: 11/09/2015, 09:57

... controlled in the body and it is essential that the circulating stem cells or transplanted stem cells have their homing and niche interacting machinery intact so as to find a new niche and maintain their ... formation and DNA binding*; PU.1 activity downregulated by RUNX1–ETO, P ML–RARα and FLT3–ITD Amino-terminal dominant negative; carboxyterminal loss of DNA binding Amino-terminal dominant negative ... and maintenance of adult HSC In addition to CXCR4, other cell-surface receptors expressed on HSC and several cell-surface adhesion molecules, including selectins and integrins, are involved in...

Ngày tải lên: 12/09/2015, 08:19

... Synovial tissue from a patient with rheumatoid arthritis showing positive staining for Ang-1 (b) Positive staining in osteoarthritis synovial tissue (c) Ang-1 staining is absent in normal synovial tissue ... upregulated in RA (86%) compared to OA (8%) and normal synovial tissues (6%) The inflammatory and vascularity scores were higher in RA synovial tissue in comparison to OA and normal In accordance ... Synovial tissue from a patient with rheumatoid arthritis showing positive staining for Ang-2 (b) Positive staining for Ang-2 in osteoarthritis synovial tissue (c) Ang-2 staining is absent in normal...

Ngày tải lên: 09/08/2014, 03:24