genes modulated by interferons and double stranded rna

... and dissolved in RNase-free water Single -stranded RNAs were allowed to anneal by mixing equal amounts of each strand, heating to 100 °C for Functional analysis of CHH by RNAi min, and cooling gradually ... obtained by Dzitoyeva and coworkers by intra-abdominal dsRNA injection FEBS Journal 273 (2006) 5669–5677 ª 2006 The Authors Journal compilation ª 2006 FEBS 5673 Functional analysis of CHH by RNAi ... amplified from the eyestalk and stomach, and another weak one from muscle (Fig 2B) In vivo CHH gene suppression using double- stranded RNA To investigate the ability of dsRNA to disrupt the CHH function...

Ngày tải lên: 23/03/2014, 10:20

... necrosis factor-alpha, and doublestranded RNA Blood 97, 1644–1652 Zamanian-Daryoush M, Mogensen TH, DiDonato JA & Williams BR (2000) NF-kappaB activation by double- stranded- RNA- activated protein ... interferon-induced double- stranded RNA- activated protein kinase induces apoptosis Virology 199, 491–496 Der SD, Yang YL, Weissmann C & Williams BR (1997) A double- stranded RNA- activated protein ... function of the interferon-induced double- stranded RNA- activated protein kinase Proc Natl Acad Sci USA 90, 232–236 Gil J & Esteban M (2000) Induction of apoptosis by the dsRNA-dependent protein kinase...

Ngày tải lên: 23/03/2014, 13:20

... of double- stranded RNA for plant virus diagnosis Plant Dis 1990, 74:255-258 12 Castillo A, Cifuentes V: Purification and Characterization of Extrachromosomal Genetic Elements of Double- Stranded ... of double- stranded RNA from plant and fungal tissues J Virol Methods 2008, 152:32-37 doi:10.1186/1743-422X-8-38 Cite this article as: Castillo et al.: Rapid isolation of mycoviral doublestranded ... visualize the band corresponding to the L-dsRNA, but it was not possible to see the band of the M-dsRNA, since it migrates at the same speed that 25S rRNA and therefore both bands overlap in...

Ngày tải lên: 11/08/2014, 21:21

... (magenta, blue and grey) in complex with its physiological RNA target (blu ⁄ green and grey) Recognition of the RNA A-form helix is mediated by contacts between by loops and and the RNA minor and major ... cloning and characterization of the human doublestranded RNA- activated protein kinase induced by interferon Cell 62, 379–390 Green SR & Mathews MB (1992) Two RNA- binding motifs in the double- stranded ... Bevilacqua PC & Chech PR (1996) Minor-groove recognition of double- stranded RNA by the double- stranded RNA- binding domain from the RNA- activated protein kinase PKR Biochemistry 35, 9983–9994...

Ngày tải lên: 19/02/2014, 17:20

... Molecular cloning and characterization of the human double- stranded RNA activated protein kinase induced by interferon Cell 62, 379–390 Hovanessian, A.G (1989) The double stranded RNA- activated protein ... mitogen-activated protein kinase and c-Jun NH2-terminal kinase by double- stranded RNA and encephalomyocarditis virus: involvement of RNase L, protein kinase R., and alternative pathways Mol Cell Biol ... of double- stranded RNA- activated protein kinase (PKR) in the cell death by influenza virus infection J Virol 70, 8128–8132 21 Takizawa, T., Tatematsu, C & Nakanishi, Y (1999) Doublestranded RNA- activated...

Ngày tải lên: 08/03/2014, 09:20

... similarity to the double- stranded RNA- binding domain and (b) proteins with similarity to the C-terminal domain of glycyl-tRNA synthetase The first group includes exclusively RNA- binding proteins ... Schultz, S.C (1998) Molecular basis of doublestranded RNA protein interactions: structure of a dsRNA-binding domain complexed with dsRNA EMBO J 17, 7505–7513 43 Nanduri, S., Carpick, B.W., Yang, Y., ... Adams, J., Micklem, D.R., Proctor, M.R., Freund, S., Bycroft, M., St Johnston, D & Varani, G (2000) RNA recognition by a Staufen double- stranded RNAbinding domain EMBO J 19, 997–1009 45 St Johnston,...

Ngày tải lên: 17/03/2014, 10:20

... SE, Mello CC: Potent and specific genetic interference by double- stranded RNA in Caenorhabditis elegans Nature 1998, 391:806-811 Lavorgna G, Dahary D, Lehner B, Sorek R, Sanderson CM, Casari G: ... overlapping genes among specific functional categories, that is, imprinted genes and DNA repair genes [1] Twenty-two out of 58 known imprinted murine genes are transcribed from both strands Frequently, ... Arabidopsis thaliana A and A' label the start and end of the sense transcript, B' and B label the start and end of the antisense transcript The total number of genes involved in group 1, and is 2,157,...

Ngày tải lên: 14/08/2014, 14:21

... of the wild-type, 4·-RNase HI and 5·-RNase HI proteins by 27.0, 14.8 and 7.0 kJÆmol)1 and 3.1, 1.7 and 0.8 m, respectively Thus, the stabilities of the 5·-RNase HI and 6·-RNase HI proteins against ... into So-RNase HI or 4·-RNase HI The stabilities of the 4·-RNase HI, 5·-RNase HI and 6·-RNase HI proteins against thermal denaturation were analysed as described for R97G-RNase HI and D136H-RNase ... was 49.1 °C for 4·-RNase HI, 52.5 °C for 5·-RNase HI and 59.2 °C for 6·-RNase HI Thus, the 5·-RNase HI protein is more stable than the wildtype and 4·-RNase HI proteins by 22.1 and 3.4 °C, respectively,...

Ngày tải lên: 18/02/2014, 13:20

... regulation of RGS1 and RGS2 expression Results RGS1 and RGS2 mRNA expression is regulated by LP and LPS BMDM were stimulated with FSL-1, a ligand for the TLR2 ⁄ heteromer, and LPS, a ligand for TLR4 ... that both BMDM and J774 cells express RGS1 and RGS2 mRNA and modulate expression after stimulation with LP and LPS Expression patterns of RGS1 and RGS2 after activation of TLR3 and TLR9 To investigate ... 12 and FEBS Journal 276 (2009) 649–659 ª 2008 Research Center Borstel Journal compilation ª 2008 FEBS S Riekenberg et al RGS are modulated by lipopeptides and LPS Relative expression of RGS1 mRNA...

Ngày tải lên: 18/02/2014, 13:20

... maintained by the opposing processes of release by the ryanodine receptor and re-uptake by SERCA Thapsigargin (TG), a selective SERCA inhibitor, can be used to deplete S ⁄ ER calcium stores by blocking ... absence of lysosomal inhibitors, and the number of GFP–LC3 FEBS Journal 274 (2007) 3184–3197 ª 2007 The Authors Journal compilation ª 2007 FEBS N R Brady et al Bcl-2 and calcium control of autophagy ... cancer [4] and neurodegenerative conditions including Alzheimer’s [5] and Parkinson’s diseases [6] In the heart, autophagy may protect against apoptosis activated by ischemic injury [7], and its...

Ngày tải lên: 07/03/2014, 09:20

... identification of 35 new miRNA genes Similarly, 42 new mouse miRNAs were found by orthology with known human miRNA precursors Added to those deposited in the miRNA registry, about 200 miRNA genes are now ... stem-loop structure and conserved position relative to other miRNAs or RefSeq genes However, the presumptive mature miRNAs hsa-miR-329 and -322 differ from their mouse and rat orthologues by four nucleotides ... FEBS miRNAs reside, in the sense orientation, in a longer form of the AY358799 mRNA, and might regulate the translation and ⁄ or stability of the mRNA AK125996 67 New human and mouse microRNA gene...

Ngày tải lên: 07/03/2014, 16:20

... growth phase by centrifugation, and ruptured by sonication Cell debris was removed by a 10 centrifugation (14 000 g) and the supernatant was used in assays ATCase activity was determined by a radiometric ... for pyr RNA, as shown by the failure of a control RNA (i.e the antisense strand to BcBL1) to bind to any concentration of PyrR tested (Fig 2A) Binding of PyrR to BcBL2 and BcBL3 in standard binding ... (protein and RNA) would be additive PyrR has no tryptophan residues and, at the concentrations FEBS Journal 275 (2008) 655–670 ª 2008 The Authors Journal compilation ª 2008 FEBS 667 RNA binding...

Ngày tải lên: 16/03/2014, 06:20

... FEBS 2503 In vivo degradation of NOS and HSP90 by calpain M Averna et al Table Levels of native and 15 kDa calpastatin species in brain and aorta of NMS and HMS rats treated with HSD for weeks ... FEBS Journal 275 (2008) 2501–2511 ª 2008 The Authors Journal compilation ª 2008 FEBS M Averna et al In vivo degradation of NOS and HSP90 by calpain B A C D Fig In vivo digestion of NOS and HSP90 ... IgG1-calpastatin (mAb 35.23) and serum l-calpain (mAb FEBS Journal 275 (2008) 2501–2511 ª 2008 The Authors Journal compilation ª 2008 FEBS M Averna et al In vivo degradation of NOS and HSP90 by calpain A B...

Ngày tải lên: 30/03/2014, 04:20

... levels of the L1 proteins encoded by the two PV Nat L1 genes (Fig 2B) The down- and up-regulated responses of the L1 protein expression from the PV Mod and Nat L1 genes to post-transfection treatment ... transcripts were assessed by quantitative RT-PCR β-tubulin transcript was analysed as an internal control Up panel: Representative electrophoresis of the L1 and tubulin mRNA qRT-PCR products Lower ... analyzed by quantitative RT-PCR for the L1 and tubulin transcripts using SYBR® Greet PCR kit (Qiagen, Australia) The specificity of RT-PCR was determined by the Rotor Gene Software and agarose...

Ngày tải lên: 18/06/2014, 18:20

... labeled by random primed Digoxigenin-11-dUTP Cotransfection of pGLu883∆Xba/LuIII Lt-Lt (lane 2), resulted in three sets of doublet bands These doublets were of ~1.8, ~1.2 and ~.8 kb These bands ... origin in the extended double stranded hairpin configuration Given the functionality of the left hairpin of LuIII as an origin of replication in the extended double stranded form a replication ... larger band migrates along side the band observed for pGLu883∆Xba likely representing the left end hairpin of LuIII in double stranded form The smaller of the two bands, of greater intensity, may...

Ngày tải lên: 19/06/2014, 08:20

... levels of the L1 proteins encoded by the two PV Nat L1 genes (Fig 2B) The down- and up-regulated responses of the L1 protein expression from the PV Mod and Nat L1 genes to post-transfection treatment ... transcripts were assessed by quantitative RT-PCR β-tubulin transcript was analysed as an internal control Up panel: Representative electrophoresis of the L1 and tubulin mRNA qRT-PCR products Lower ... analyzed by quantitative RT-PCR for the L1 and tubulin transcripts using SYBR® Greet PCR kit (Qiagen, Australia) The specificity of RT-PCR was determined by the Rotor Gene Software and agarose...

Ngày tải lên: 20/06/2014, 01:20

... labeled by random primed Digoxigenin-11-dUTP Cotransfection of pGLu883∆Xba/LuIII Lt-Lt (lane 2), resulted in three sets of doublet bands These doublets were of ~1.8, ~1.2 and ~.8 kb These bands ... origin in the extended double stranded hairpin configuration Given the functionality of the left hairpin of LuIII as an origin of replication in the extended double stranded form a replication ... larger band migrates along side the band observed for pGLu883∆Xba likely representing the left end hairpin of LuIII in double stranded form The smaller of the two bands, of greater intensity, may...

Ngày tải lên: 20/06/2014, 04:20

... III RNase H-free reverse transcriptase (Invitrogen Corp.), 200 ng random hexamers, and μg total RNA The target cDNAs were amplified for 30 cycles (CYP17A1 and StAR) and 25 cycles (36B4, internal ... expression, and androgen production in theca cells These results suggest that LH stimulates CYP17A1 mRNA expression and androgen production in theca cells via activation of the PI3K/Akt pathway, and ... StAR, and 36B4 (internal control) primers using total RNA isolated from the cells The products were fractionated on 1% agarose gel and stained with ethidium bromide The mRNA levels of CYP17A1 and...

Ngày tải lên: 20/06/2014, 07:20

... Camper SA, Weigert M: The site and stage of anti-DNA B-cell deletion Nature 1995, 373:252-255 Rekvig OP, Nossent JC: Anti -double- stranded DNA antibodies, nucleosomes, and systemic lupus erythematosus: ... determine when and why the switch from nonpathogenic and clinically irrelevant to pathogenic and clinically relevant antibodies occurs If anti-DNA antibody pathogenicity is determined by the interaction ... anti-dsDNA antibodies and clinical manifestations is not required in SLE classification and diagnosis For example, the previous presence of anti-dsDNA antibodies (by any assay) and accordingly a...

Ngày tải lên: 09/08/2014, 06:22

... cells and CD8+CD45RO+ICOS+ cells, respectively, were as follows: active SLE, 1.80 and 1.25; inactive SLE, 0.45 and 0.40; and normal control individuals, 1.10 and 0.50 *P < 0.05, **P < 0.01, and ... cells and B cells/well), and were cultured in the presence of various stimuli for seven days Culture supernatants were collected and stored at -80°C until assayed for anti-dsDNA antibody and total ... CD4+CD45RO+ ICOS+ cells and CD8+CD45RO+ICOS+ cells, respectively, were as follows: active SLE, 71.3% and 33.2%; inactive SLE, 11.1% and 6.2%; and normal control individuals, 42.8% and 19.2% The MFI...

Ngày tải lên: 09/08/2014, 07:20