generation of mouse models

... job of the speech recognizer hard The resultant transcriptions have a word error rate of about 40% This high error rate implies that many wrong deletions of actual words and wrong insertion of ... up so that i mean Figure 1: Partial transcript of a help desk dialog minutes For 125 of these calls, call topics were manually assigned Generation of Domain Model Fig shows the steps for generating ... taxonomy as part of the model viz (1) average call duration(in seconds), (2) average transcription length(number of words) (3) average number of speaker turns and (4) number of calls We observed...

Ngày tải lên: 31/03/2014, 01:20

... versions of digital models Thus, we focus on the fabrication of LEGO models in this thesis Building 3D LEGO models manually, however, requires considerable trial-and-error even for small models ... structurally strong LEGO models Chapter Introduction Recently, the generation of 3D models for fabrication has attracted great interest from the computer graphics community The idea of moving creations ... problem of automatically generating LEGO models similar in appearance to the given 3D digital models Building 3D LEGO models manually requires considerable trial-and-error even for small models...

Ngày tải lên: 22/09/2015, 15:18

... Significance of findings in Balb/c and Swiss outbred mice 74 2.3 Mouse GO versus Human GO 74 2.4 Clinical relevance of differences in cytokine profile in Mouse model 75 2.5 Limitation of current ... Thyrotropin receptor viii List of Figures Figure An illustration of the physiologic control of thyroid function Figure Activation of adenylyl cyclase following binding of TSH to TSHR Figure The Thyrotropin ... tabulation of FACS status in the strains of mice immunized with TSHR Table 18 Cross tabulation of TBII status in the strains of mice immunized with TSHR Table 19 54 Relative fold change of Th2 cytokines...

Ngày tải lên: 07/10/2015, 09:55

... have to be tested in vivo by proof -of- concept experiments in reliable mouse models of breast cancer metastasis In the future it will be necessary to generate mouse models that more accurately recapitulate ... expression profiling experiments with patient samples are able to modulate breast carcinogenesis in transgenic mouse models, for example in the wellcharacterized MMTV-Neu and MMTV-PyMT mouse models of ... similarity of this Page of 11 (page number not for citation purposes) Available online http://breast-cancer-research.com/content/8/4/212 Table Transgenic mouse models of breast cancer metastasis TG mouse...

Ngày tải lên: 22/03/2014, 17:20

... of motor dysfunction, we also studied gait dynamics in the SOD1 G93A transgenic mouse model of ALS [39] to compare gait variability in mouse models of basal ganglia disease to a mouse model of ... MPTP-treated mouse Gait signals in a MPTP-treated mouse Gait signal of the right hind limb of a MPTP-treated mouse superimposed over the gait signal of the right hind limb of a saline-treated mouse ... inability of the mice to walk at all on the treadmill belt SOD1 G93A transgenic mice To compare gait variability in the MPTP and 3NP mouse models of basal ganglia disease to a mouse model of motor...

Ngày tải lên: 19/06/2014, 10:20

... production by brain slices from transgenic mouse models of AD at 15 months of age Cytokine production by brain slices from transgenic mouse models of AD at 15 months of age Freshly harvested brain slices ... production by brain slices from transgenic mouse models of AD at 15 months of age Cytokine production by brain slices from transgenic mouse models of AD at 15 months of age Freshly harvested brain slices ... around areas of plaque deposition [4-7] Chronic administration of ibuprofen can reduce plaque pathology and brain Aβ levels in these animal models of AD [8,9] There are numerous reports of increased...

Ngày tải lên: 19/06/2014, 22:20

... expression profiles in mouse models of AD and of cerebral amyloid angiopathy Our data demonstrate that genes typical of alternative activation are clearly expressed in AD and in a mouse model of amyloid ... profile Alternative activation gene profiles in mouse models of AD Since alternative activation could be demonstrated in vitro using the expression of specific genes, we determined if mouse models ... Figure Alternative activation genes in mouse models of amyloid pathology Alternative activation genes in mouse models of amyloid pathology The transcripts of activation-related genes were measured...

Ngày tải lên: 19/06/2014, 22:20

... expression of SV40 TAg to the epithelium of the female reproductive tract by microinjection of this construct into the male pronucleus of 0.5-day old embryos to generate transgenic animals While 18 of ... alternative method of generation Genetically induced granulosa cell tumor (GCT) models Granulosa cell tumors (GCT) represent 2-5% of all OCs [53] arising from the granulosa cells of the ovary, which ... TAg immortalization of cultured human OSE cells eliminated the presence of CA-125 [69], one of the current diagnostic markers for EOC [70] To understand the complexity of OC, a mouse model representing...

Ngày tải lên: 20/06/2014, 07:20

... three isoforms of IL-1Ra protein, which possess inhibitory activity against IL-1, are synthesized by alternative splicing of a single gene, we produced mice deficient in all three isoforms of IL1Ra ... group of genes is involved in the pathogenesis of arthritis in both models, and we extracted commonly changed genes in both models To extract commonly changed genes, we applied the principle of ... indicating that the expression of many genes changed in both models (Figure 1) We thought that the same group of genes should be involved in the development of arthritis in both models at the effector...

Ngày tải lên: 09/08/2014, 08:22

... deviation of three experiments MFI, mean fluorescence index in the culture supernatants of CD11c+ DCs of tolerized mice than in the supernatants of CD11c+ DCs of CIA mice cultured in the presence of ... Ethics Committee at the Catholic University of Korea Preparation of type II collagen Bovine CII was kindly provided by Professor Andrew Kang of the University of Tennessee CII was extracted in its ... suppression by DCs of the expansion and differentiation of effector T cells and promotion of T-cell tolerance remain elusive One regulatory mechanism of DCs is the suppression of proliferation...

Ngày tải lên: 09/08/2014, 10:22

... development and evolution of ACPA responses in rodent models of RA and MS, with the objective of gaining further insights into the aetiology, evolution and potential pathogenic role of such responses ... on mouse serum collected from control and CIA mice The protocol was adapted from the manufacturer's suggested procedure, with substitution of 1:150 dilutions of mouse serum and a goat-anti -mouse ... development and/or clinical progression of RA The purpose of these studies was to characterise the development and evolution of ACPA responses in rodent models of RA and MS, to gain insights into...

Ngày tải lên: 09/08/2014, 13:22

... 129B6/FVB mouse strains (Additional file 1) Hierarchical clustering of the expression of these miRNAs across all of the mouse mammary tumor models indicated that the expression levels of the 22 ... particular, models with loss of function of p53, Rb or BRCA1 share molecular features with the human basal-subtype of breast cancer [14] In this study, we have performed global miRNA expression profiling ... the inverse expression of mRNA targets predicted in silico These analyses have identified potential in vivo mRNA targets of specific miRNAs in the context of these models of mammary cancer To our...

Ngày tải lên: 09/08/2014, 23:20

... 129B6/FVB mouse strains (Additional file 1) Hierarchical clustering of the expression of these miRNAs across all of the mouse mammary tumor models indicated that the expression levels of the 22 ... particular, models with loss of function of p53, Rb or BRCA1 share molecular features with the human basal-subtype of breast cancer [14] In this study, we have performed global miRNA expression profiling ... the inverse expression of mRNA targets predicted in silico These analyses have identified potential in vivo mRNA targets of specific miRNAs in the context of these models of mammary cancer To our...

Ngày tải lên: 09/08/2014, 23:20

... mechanisms of action - a glucocorticoid (budesonide), a phosphodiesterase (PDE) inhibitor (roflumilast) and a statin (fluvastatin) As one of the primary functions of preclinical disease models is ... 3mg/kg Roflumilast Sham + Vehicle CS + Vehicle CS + 3mg/kg Roflumilast C * Sham + Vehicle Figure The effect of roflumilast on chronic CSE-induced inflammatory cell infiltrate After 14 weeks of CSE, ... is to assess the potential efficacy of candidate compounds, ideally one would examine the same endpoints in the models as in the clinic Typically, early proof -of- concept studies for COPD anti-inflammatory...

Ngày tải lên: 12/08/2014, 11:22

... or NOG mouse model Rag2-/- c-/- mouse model SCID-hu Thy/Liv mouse model hu-PBL SCID mouse model NOD/SCID 2-microglobulin-/- mouse model Humanized Mouse Model History Figure of humanized mouse ... development of a variety of immunocompromised strains of mouse [69] Most of these animals are the result of adjustments made to the 1983 CB17-scid mouse model It was in this model that engraftment of ... mutation All of these mouse models have developed over time to various degrees of accuracy and efficiency of xenotransplantation of human cells/tissues as well as the development of a functioning...

Ngày tải lên: 12/08/2014, 23:22

... of therapeutic anti-tumor immunity Induction of therapeutic anti-tumor immunity Mice (five per group in both the CT26 and 4T1 tumor models) were treated i.v once a week for weeks with 20 μg of ... administer to humans [8-10] A number of studies have reported the use of plasmid DNA-based vaccines for eliciting anti-tumor immunity in mice [11-14] The current generation of plasmid DNA-based cancer ... range of 300–325 nm (The preparation of liposome DNA complexes was supported by The Chemical Laboratories of the State Key Laboratory for Biotherapy) Therapeutic anti-tumor immunity and tumor models...

Ngày tải lên: 14/08/2014, 19:22

... Guan Shou Ping iii TABLE OF CONTENTS DECLARATION ii ACKNOWLEDGEMENTS iii TABLE OF CONTENTS iv SUMMARY viii LIST OF TABLES x LIST OF FIGURES xi LIST OF ABBREVIATIONS xiv LIST OF PUBLICATIONS AND CONFERENCE ... Burden of Asthma 1.2.2 Pathophysiology of Asthma 1.2.2.1 Inflammatory and Structural Cells 1.2.2.2 Mediators of Asthma 22 1.2.2.3 Airway Hyperresponsiveness (AHR) 32 1.2.3 The mouse model of asthma ... system 77 Figure 3.3 Type of cells found in BAL fluid of mice 80 Figure 3.4 Type of cells found in BAL fluid of mice 81 Figure 3.5 The FinePointe™ system 86 Figure 4.1 Effects of andrographolide and...

Ngày tải lên: 09/09/2015, 10:06

... Generation of Hepatocyte-like cells from Mouse Embryonic Stem (ES) and Bone Marrow (BM) Cells YIN YIJUN A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY OF MEDICINE DEPARTMENT OF ... 2004 Feb [Epub ahead of print] xiv ABSTRACT OF THESIS ENTITLED Generation of Hepatocyte-like cells from Mouse Embryonic Stem (ES) and Bone Marrow (BM) Cells The paucity of hepatocytes available ... derivation of mesodermal tissues from ES cells, much of the efforts were focused on the derivation of hematopoietic lineages A large number of studies has documented the generation of primitive...

Ngày tải lên: 16/09/2015, 12:10

... windows 7.5x (Windows) Microsoft Systems Management Server, Microsoft System Center Operations Manager, and Microsoft Operations Manager 2005 36 The New Generation of System x and BladeCenter ... performance gains of the Intel Xeon 5500 Series Processors enables unprecedented opportunities for business All of the new generation servers boast processor performance improvements of: – Up to 3.5x ... one part of their business they don’t need to worry about This is the investment protection that BladeCenter offers our customers *Available as 32GB and 50GB drives 16 The New Generation of System...

Ngày tải lên: 19/10/2013, 03:15

... Activity 4.3: Value of Information Models Exercise 1: Identifying the Value of Information Models ! Identify the value of information models Think of how you have used information models in previous ... that informational modeling provides to the design process and specifically to the analysis step of conceptual design The instructor will write your answers on a flip chart ...

Ngày tải lên: 10/12/2013, 16:16