generation of families of construct variants using golden gate shuffling

Báo cáo khoa học: "Automatic Generation of Information-seeking Questions Using Concept Clusters" ppt

Ngày tải lên : 23/03/2014, 17:20

... clusters Currently we use the reciprocal of the size of the cluster The second part is the average pairwise distance between answers N is the total number of classes of the answers Next we describe in ... subtopic of cluster Ci and m is the size of Ci We designed our system to take a question and its corresponding list of answers as input and then retrieve Google snippet documents for each of the ... ’MAP’ column is the mean of average precisions over the set of clusters The ’P@1’ column is the precision of the top one cluster while the ’P@3’ column is the precision of the top three clusters2...

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Báo cáo sinh học: " Generation of high-titer viral preparations by concentration using successive rounds of ultracentrifugation" doc

Ngày tải lên : 18/06/2014, 22:20

... μg of DNA (28.26 μg of the H1 shRNA hairpin vector; 18.3 μg of pMDLg/ pRRE; 9.86 μg of pMD2.G and 7.04 μg of pRSV Rev) and 229.4 μL of M Calcium solution in a total volume of 3.7 mL The transfection ... flow cytometry of transduction by retrovirus following concentration using different numbers of rounds of centrifugation μL of retrovirus was added for each transduction B Titration of concentrated ... one well of a 6-well plate in mL of medium containing μg/mL of polybrene (Sigma) After hours virus was washed off the NIH/3T3 cells and fresh medium was added After 48 hours the number of cells...

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báo cáo hóa học:" Generation of high-titer viral preparations by concentration using successive rounds of ultracentrifugation" pot

Ngày tải lên : 20/06/2014, 04:20

... μg of DNA (28.26 μg of the H1 shRNA hairpin vector; 18.3 μg of pMDLg/ pRRE; 9.86 μg of pMD2.G and 7.04 μg of pRSV Rev) and 229.4 μL of M Calcium solution in a total volume of 3.7 mL The transfection ... flow cytometry of transduction by retrovirus following concentration using different numbers of rounds of centrifugation μL of retrovirus was added for each transduction B Titration of concentrated ... one well of a 6-well plate in mL of medium containing μg/mL of polybrene (Sigma) After hours virus was washed off the NIH/3T3 cells and fresh medium was added After 48 hours the number of cells...

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Báo cáo tin học: "Using algebraic properties of minimal idempotents for exhaustive computer generation of association schemes" pps

Ngày tải lên : 07/08/2014, 15:23

... properties of association schemes are relevant to our generation algorithms For actual proofs of the properties listed in this section, and for further information on the subject of association ... With every relation Ri of Ω we may associate a 0–1-matrix Ai of size n × n as follows : rows and columns of Ai are indexed by the elements of V and the entry at position x, y of Ai is deﬁned to be ... check: lexical ordering of the rows of M and clique checking [9, 12] A more extensive discussion of the techniques we use for isomorph-free exhaustive generation algorithms of association schemes...

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Báo cáo khoa học: " Investigation of tumor hypoxia using a twoenzyme system for in vitro generation of oxygen deficiency" pot

Ngày tải lên : 09/08/2014, 09:20

... [10] Aim of the present work is to investigate the effects of rapidly induced hypoxia on cellular processes using the enzymatic GOX/CAT system in comparison to the established method of hypoxia ... investigation of the effects of the hypoxia-targeted prodrug KS119 [9,17] Furthermore, Zitta et al used GOX/CAT for rapidly induction of hypoxia and investigated the influence of mild hypothermia ... increase of gene expression over 24 h Our results are in concert with the results of previous studies using the GOX/CAT system [10] Millonig et al have shown that a fast onset of hypoxia using the...

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Báo cáo y học: "Targeted next-generation sequencing of a cancer transcriptome enhances detection of sequence variants and novel fusion transcript" ppt

Ngày tải lên : 09/08/2014, 20:20

... typically for the purpose of enriching exonic DNA of interest Although targeted sequencing of genomic DNA facilitates mutation-discovery/ profiling, it is unable to interrogate the myriad additional ... to of 887 target transcripts 220,151 6,364,131 On-target specificity 5% Figure Preservation of expression levels of target transcripts in hybrid selection Preservation of expression levels of ... also enabled the identification of a greater number of alternatively spliced isoforms of these genes Considering all possible logical intragenic combinations of exons annotated in RefSeq, 70,344...

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Báo cáo y học: "Effective detection of rare variants in pooled DNA samples using Cross-pool tailcurve analysis" ppsx

Ngày tải lên : 09/08/2014, 23:20

... identification of rare variants in large sample cohorts For validation of this strategy, we present data from sequencing 12 indexed libraries of 40 samples each (total of 480 samples) using a single lane of ... a trim value of 25, which is the approximate length of our primers Reliable detection of rare variants in pooled samples 10 Using SERVIC4E, we identified 68 unique variants (total of 333 among ... detection of rare variants in pooled samples We validated this strategy using Illumina sequencing data from an additional independent cohort of 480 samples Compared to publicly available software,...

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Báo cáo y học: "utomated generation of massive image knowledge collections using Microsoft Live Labs Pivot to" doc

Ngày tải lên : 10/08/2014, 09:22

... benefits of DZI generation, which are expansions and implementation of analytic and statistical functions that will extract meaningful information from Pivot collections Page of 11 A lot of effort ... massive image collections of mouse brain structure that consists of the most comprehensive sets of recombinant inbred strains, including BXD, LXS, AXB, BXH, and CXB for studies of the genetic control, ... extensible markup language (XML) consists of a set of rules to describe structured data to be displayed in Pivot collection Page of 11 The CXML file contains the set of categories and types associated...

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Báo cáo y học: "Rapid generation of an anthrax immunotherapeutic from goats using a novel non-toxic muramyl dipeptide adjuvant." docx

Ngày tải lên : 11/08/2014, 10:23

... capable of disrupting several cell signaling cascades; however, its specific mode of action is still unclear [17,18] Disruption of the binding of PA to ATR or LF would disrupt internalization of functional ... TCEC50 equivalent to a total of 50 ng LeTx per well Generation and evaluation of anti-PA83 caprine immunoglobulin One goal of this study was to produce large volumes of high titer, hyper-immune ... with goat IgG, we have explored the use of F(ab')2 antibodies lacking the Fc region of the IgG molecule The Fc region of the IgG is involved in the activation of complement, and patients with a pre-developed...

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Báo cáo y học: "A new approach for the large-scale generation of mature dendritic cells from adherent PBMC using roller bottle technology" ppt

Ngày tải lên : 11/08/2014, 10:23

... of phenotypically mature mDC from adherent monocytes using ITIP in roller bottle cultures in comparison to Generationcultures Generation of phenotypically mature mDC from adherent monocytes using ... comparison of phenotypically mature mDC from adherent monocytes using the Pittsburgh Protocol in roller bottle cultures in Generation Generation of phenotypically mature mDC from adherent monocytes using ... of roller bottles on a roller apparatus at low speed After removal of the non-adherent cells, DC cells are generated using culture medium containing GMCSF and IL-4 and matured using any one of...

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Báo cáo y học: " Asthma families show transmission disequilibrium of gene variants in the vitamin D metabolism and signalling pathway" pot

Ngày tải lên : 12/08/2014, 16:20

... from 224 families where 872 serum measurements of 25-OH-D3, 876 1,25- OH2-D3 and 934 total IgE measurements could be performed After exclusion of non-white families 903 individuals from 201 families ... relevant [11] Most SNPs were genotyped using MALDI-TOF mass spectrometry of allele-specific primer extension products generated from amplified DNA Page of 11 (page number not for citation purposes) ... quartile of outlier of 25-OH-D3 serum levels in 872 participants of the German Asthma Family Study with white skin colour Median,by monthandexamination Median, quartile and outlier of 25-OH-D3...

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Báo cáo y học: "Prevention of Pleural Adhesions Using a Membrane Containing Polyethylene Glycol "

Ngày tải lên : 25/10/2012, 11:00

... the Experimental Animals Laboratory of Dokuz Eylul University Faculty of Medicine The purpose of using rats is easy availability, safety, and the high ratio of repeating the experiment Design ... thoracotomy by using a hyaluronate-based absorbable membran in rats [6] Also, Getman et al achieved the same effect by using haemostatic membrane [7] The present study investigates the efficacy of Prevadh® ... Adhesions were scored as shown below: A) Length of adhesion: The length of adhesion before the first thoracotomy incision (mm) B) Scoring of the intensity of adhesion between the parietal pleura and...

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DETOXIFICATION OF TRICHLOROETHYLENE (TCE) USING SOLAR LIGHT/TiO2 IN A UV CONCENTRATING RADIATION SYSTEM

Ngày tải lên : 05/09/2013, 08:40

... effect of dark reaction (the effect of adsorption of TCE on the TiO2 powder), as well as the photolysis of TCE by solar radiation only in the absence of TiO2 Figure showed the degradation ratio of ... instead of simply transferring them to another medium (Ahmed et al., 1984; Glaze et al., 1993) The purpose of our study is to investigate the feasibility of the solar detoxification using TiO2 ... TiO2) Effect of the TiO2 regeneration We conducted an experiment that used TiO2 particle at the end of a single day runs, which was filtered out using an aspirator, washed with liter of deionized...

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$Polystyrene (PS) waste plastic conversion into aviation /kerosene category of fuel by using fractional column distillation process$

Polystyrene (PS) waste plastic conversion into aviation /kerosene category of fuel by using fractional column distillation process

Ngày tải lên : 05/09/2013, 15:28

... (broad) C-CH3 C-CH3 C-C=-C-C=-CH C-C=-C-C=-CH Non-Conjugated Non-Conjugated Non-Conjugated Non-Conjugated Non-Conjugated Non-Conjugated Non-Conjugated CH3 Acetates -CH=CH2 -CH=CH2 -CH=CH-(cis) Aviation ... Journal of Energy and Environment (IJEE), Volume 3, Issue 6, 2012, pp.871-880 polyethylene and most of them is in the form of containers and packaging materials The extent of conversion of plastics ... An interesting method of polymers utilization is cracking, similar to the visbreaking of vacuum residue [8] or catalytic cracking of vacuum gas oil (VGO) in the presence of various zeolites containing...

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Experimental study of passive cooling of building facade using phase change materials to increase thermal comfort in buildings in hot humid areas

Ngày tải lên : 05/09/2013, 17:03

... inclusion of PCMs depend on the melting temperature of the PCM, the type of PCM, the percentage of PCM used inside the conventional material, the climate, design and orientation of construction of the ... each of cubic feet using RCC slab, hollow bricks & cement mortar and tile roof, hollow bricks & cement mortar were constructed to test the performance of PCM integrated walls with different roofing ... energy storage (TES) of a building Using the granules of microencapsulated PCM as part of the aggregate for the concrete, the PCM is embedded simply and effectively within the construction material...

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Tài liệu tiếng anh Điện tử công suất mạch MERS Improved performance of induction motor using magnetic energy recovery switch

Ngày tải lên : 15/10/2013, 16:10

... Applications of voltage control of induction motor were investigated Stability problem because of self-excitation of series com- 924 Authorized licensed use limited to: TOKYO INSTITUTE OF TECHNOLOGY ... quarter of the resonance period of L and C of the MERS, vmers, becomes sinusoidal, consequently the terminal voltage,vterm, also becomes sinusoidal Fig shows simulated waveforms of 'dc-offset ... cycle of the ac power source and controlled synchronously The phase difference between the voltage of the power source and the switching can be controlled The principle of the operation of the...

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The New Generation of x86 Server Innovation

Ngày tải lên : 19/10/2013, 03:15

... windows 7.5x (Windows) Microsoft Systems Management Server, Microsoft System Center Operations Manager, and Microsoft Operations Manager 2005 36 The New Generation of System x and BladeCenter ... performance gains of the Intel Xeon 5500 Series Processors enables unprecedented opportunities for business All of the new generation servers boast processor performance improvements of: – Up to 3.5x ... one part of their business they don’t need to worry about This is the investment protection that BladeCenter offers our customers *Available as 32GB and 50GB drives 16 The New Generation of System...

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Analysis and design of control systems using MATLAB

Ngày tải lên : 01/01/2014, 18:43

... of the real part of G(jω) versus the imaginary part of G(jω) Pole of a Transfer Function: The root (solution) of the (characteristic) equation obtained by setting the denominator polynomial of ... system’s equations of motion are rewritten as a system of first-order differential equations, each of these differential equations consists of the time derivative of the one of the state variables ... logarithm of the magnitude of the transfer function is plotted versus the logarithm of ω, the frequency The phase, φ, of the transfer function is separately plotted versus the logarithm of the frequency...

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03 analysis of mechanical systems using interval computations applied to finite element methods o dessombz

Ngày tải lên : 12/01/2014, 21:45

... point of view, and the others often encounter convergence problems Moreover, only the mean value and the moments (often the variance only) are known, and since the density of probability of the ... convergence of the iterative scheme The smaller the spectral radius of |[G]|, the faster the convergence of the algorithm and the smaller the overestimation of the solution 4.2 TEST OF THE NEW ... Validated solutions of linear equations Technical Report 620, Institute of Computer Science, Academy of Sciences of the Czech Republic 22 J Rohn and G Rex, feb 1996 Enclosing solutions of linear equations...

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Tài liệu Fundamentals of Computer Science using Java doc

Ngày tải lên : 17/01/2014, 06:20

... mind ■ Software Engineering Development of large-scale software is a very complex task typically carried out by a team of software development professionals Although there are a number of different ... phase of software development in which the classes responsibility of testing (one of the most overlooked phases of defined in the design phase are development—just consider some of the software ... 256MB of RAM of RAM (or about 256 million bytes of memory2) *This section represents optional material 11 CHAPTER • COMPUTING FUNDAMENTALS TABLE 1.1 Powers of Number of bits Values Number of possibilities...

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