generation and hepatic differentiation of human ips cells

... progression and embryonic development [30] We found evidence of transdifferentiation by MET in the process of hepatic differentiation of AT-MSCs No previous report has demonstrated evidence of transdifferentiation ... mechanism of hepatic differentiation from AT-MSCs This report provides evidence that the transcriptome and signal pathways of AT-MSC-Hepa are similar to those of human primary hepatocytes and that hepatic ... the process of hepatic differentiation from AT-MSCs Although further investigations are required to elucidate the molecular mechanism of transdifferentiation of AT-MSCs into hepatic cells, the...

Ngày tải lên: 30/03/2014, 04:20

... tnecseroulf gniwollof ehT SBP htiw semit eerht desnir erew selpmas ehT Co73 ta h rof sllec eht htiw detabucni erew seidobitna yramirp ehT ylevitcepser ,PAFG rof 002 : dna ESN rof 002 : ta ares ... 001-X notirT %4.0 neht dna ,nim rof enoteca %001 ,nim 01 rof lonahtem %001 dloc eci htiw detaert neht erew yehT erutarepmet moor ta nim 01 rof SBP ni edyhedlamrof %7.3 htiw dexif neht dna SBP ... llec gnimrofrep dna esu lacinilc rof SBF gnisu tuohtiw oviv xe sCSM dnapxe ot sdohtem dnif ot deen ew dna ,hcraeser ruo rof SBF desu ew ,yduts tneserp ni ,revewoH noitarefilorp sCSM rof noitidnoc...

Ngày tải lên: 07/08/2014, 18:21

... osteogenic differentiation of human mesenchymal stem cells Exp Cell Res (1):22-37 101 Salasznyk RM, Klees RF, Boskey A, et al 2007;Activation of FAK is necessary for the osteogenic differentiation of human ... mammary gland involution J Cell Biol (4):693-703 110 Zizola CF, Julianelli V, Bertolesi G, et al 2007;Role of versican and hyaluronan in the differentiation of 3T3-L1 cells into preadipocytes and mature ... stem cells Exp Biol Med (Maywood) (6):507-520 115 Aratani Y, Kitagawa Y 1988;Enhanced synthesis and secretion of type IV collagen and entactin during adipose conversion of 3T3-L1 cells and production...

Ngày tải lên: 11/09/2015, 09:16

... progenitor cells (MAPCs), are the stem cells Page of 15 forming bone marrow [33] Their role is maintenance and turnover of blood cells and immune system The high rate of regeneration of the liver, ... markers typical of mesenchymal cells (proteins such as CD105, CD73, and CD90) and the absence of markers characteristic of hematopoietic cells, monocytes, macrophages, or B cells; and their capacity ... protuberance of hair follicle and which are capable of self-renewal for long time periods and differentiation into specialized cells, and transient amplifying cells, distributed throughout basal lamina and...

Ngày tải lên: 18/06/2014, 16:20

... (lane 11), and, when anti-Sp3 IgG was used, bands C2 and C3 disappeared and bands SC3a and SC3b appeared (lane 12) Coaddition of the two antibodies resulted in the loss of bands C1, C2, and C3 (lane ... Sp1 and Sp3, and protein levels and binding of Sp1 and Sp3 are increased in PMAtreated cells These findings indicate that Sp1 and Sp3 play a pivotal role in the transcriptional activation of DNase ... (Fig 4A, lanes and 9) These results suggest that both Sp1 and Sp3 are able to bind to the GC boxes and that binding of Sp1 forms complex C1 and binding of Sp3 forms complexes C2 and C3 PMA treatment...

Ngày tải lên: 23/03/2014, 17:21

... impairs the differentiation and function of dendritic cells J Immunol 2005, 174:7764-7772 Li L, Li SP, Min J: Hepatoma cells inhibit the differentiation and maturation of dendritic cells and increase ... HCC cells used for fusion expressed high levels of WT1 and HLA-ABC and low levels of CEA but not HLA-DR, CD80, CD86, and CD83 molecules (Figure 1B, 2A, and 5A) Fusions of DCs to the HCC cells ... of fusion cells and have a negative impact in the stimulation of T cells To determine the induction of WT1-specitic CD8+ T cells, a pentameric assay of soluble class I MHC-peptide com- Page of...

Ngày tải lên: 18/06/2014, 15:20

... either EB or adult cells (Figure 6, panel A) The difference in the expression of miR-200c, miR-302b, and miR-367 between hES cells and EB, and between hES cells and adult cells was significant ... in adult cells (Figure 6, panel C) The differences in expression of these miRNA expression between adult cells and hES cells and between adult cells and EB were significant (P < 0.05) Of note, ... expression of miR-520b was 26-fold greater in hES cells then in EB cells (P < 0.05) and it was detected only in two types of adult cells HMVEC and HUVEC Differences in the expression of EB signature...

Ngày tải lên: 18/06/2014, 15:20

... chondrogenic differentiation of mesenchymal progenitor cells and to up regulate the levels of type II collagen and aggrecan in chondrocytes and chondroprogenitor cells [1,7-11] During development of the ... proximity of the cells to the joint space and the bone Page 10 of 15 (page number not for citation purposes) respectively, alignment of the chondrocytes along the arcades of Benninghoff [29] and by ... of primary MSCs and to investigate whether levels and extent of hypertrophy in vitro is influenced by the choice of transgene Materials and methods Construction and preparation of recombinant...

Ngày tải lên: 09/08/2014, 14:22

... phosphorylation status of Akt and ERK for stage III cells There was a baseline of Akt and ERK phosphorylation without adding ECM FN and LAM increased phosphorylation of Akt and ERK, and LAM had greater ... expression and survival of adherent cells [17] FN and LAM constitute the major ECM of pancreas islet cells Laminin-1 has been reported to promote the differentiation of fetal mouse pancreatic b -cells ... mechanism of pancreatic islets FN and LAM enhances activation of Akt and ERK The ECM bind to cells by activating signaling molecules such as Akt and ERK Therefore, we analyzed the effect of FN and...

Ngày tải lên: 10/08/2014, 05:21

... smooth muscle and endothelial cells To study the effects of HIV-1 on the differentiation of these cells, the interaction of HIV-1 and recombinant gp120 on MSC differentiation to adipogenic and endothelial ... activate C/EBP a and PPARg inducing the complete differentiation to mature adipocyte with the expression of late differentiation markers such as adipsin and UCP-1 The analysis of C/EBP b and δ mRNA ... USA) and employed between passages and To induce adipogenic differentiation, confluent cells were cultured as follows: three cycles of days induction medium and days maintenance medium of hMSC...

Ngày tải lên: 13/08/2014, 01:20

... detailing the derivation and maintenance of induced pluripotent stem (iPS) cells (Takahashi et al 2007; Takahashi and Yamanaka 2006) iPS cells are generated from mouse and human adult fibroblasts ... Sumitha, Aji and Tamanna, and Susan, thank you for the joy you bring to my life and my world Pappa and Mamma, thank you for your love and unconditional support My grandparents and the rest of my extended ... Pancreas and diabetes The pancreas consists of Acinar cells which perform its exocrine functions and clusters of cells called Islets of Langerhans which perform its endocrine functions Acinar cells...

Ngày tải lên: 04/10/2015, 16:03

... properties of HCII Here, we report on the structure of posttranslational modifications of plasma-derived and recombinant human HCII from CHO cells and their effects on heparin binding MATERIALS AND METHODS ... Amino-acid sequence and tryptic peptides of human HCII The peptides indicated below were detected by MALDI-TOF- and/ or ESI-MS (see Figs and 2, respectively) in tryptic digests of human HCII isolated ... were isolated at random, exposed to 0.05 lM MTX, expanded, and checked for the presence of human HCII mRNA by RT-PCR Positive cell lines were picked and after several rounds of selection with...

Ngày tải lên: 08/03/2014, 22:20

... CP-870,893 on T cell stimulatory capacity of B cells Effect of3 Effect of CP-870,893 on T cell stimulatory capacity of B cells Purified CD19+ B cells from each of donors were stimulated as described ... CP-870,893 on B cell activation and B cell stimulation of T cells, and we analyzed the effects of co-stimulating B cells with the TLR9 agonist CpG ODN 2006 Materials and methods In mice, agonist ... CD19+ B cells and cultured in the presence of varying concentrations of either CP-870,893 or negative control hIgG2 Cells were analyzed by flow cytometry for viability and expression of cell...

Ngày tải lên: 18/06/2014, 15:20

... (50%) are females and 50% are males C5a promotes the expression of IL-22 and IL-17 from human T cells in vitro To study the role of C5a on human CD4 + T cells, we used ELISA and intracellular ... Expression of IL-22 and IL-17 from Human T cells and its Implication in Age-related Macular Degeneration Journal of Translational Medicine 2011 9:111 Submit your next manuscript to BioMed Central and ... observation of elevated C5a expression in the serum of AMD patients [15,16], we found significantly increased levels of IL-22 and IL-17 in the sera of AMD patients, suggesting possible roles of IL-22 and...

Ngày tải lên: 18/06/2014, 22:20

... Jak-Stat and MAPK pathways in Huh7 cells, HCV replicon cells lines, and primary human hepatocytes Materials and methods Cells and chemicals Human hepatoma Huh7 cells were grown in DMEM containing ... regulation of differentiation, cell growth, and responses to cytokines, chemokines and stress The core element in MAPK signaling consists of a module of kinases, named MKKK, MKK, and MAPK, which ... Effect of1 Effect of acute ethanol on the Jak-Stat pathway A, high physiological doses of ethanol activate the ISRE Huh7 cells were transfected with 0.7 µg of pISRE-luc, and 24 hours later, cells...

Ngày tải lên: 19/06/2014, 08:20

... Jak-Stat and MAPK pathways in Huh7 cells, HCV replicon cells lines, and primary human hepatocytes Materials and methods Cells and chemicals Human hepatoma Huh7 cells were grown in DMEM containing ... regulation of differentiation, cell growth, and responses to cytokines, chemokines and stress The core element in MAPK signaling consists of a module of kinases, named MKKK, MKK, and MAPK, which ... Effect of1 Effect of acute ethanol on the Jak-Stat pathway A, high physiological doses of ethanol activate the ISRE Huh7 cells were transfected with 0.7 µg of pISRE-luc, and 24 hours later, cells...

Ngày tải lên: 20/06/2014, 04:20

... overexpression of Bcl-xL is associated with radioresistance of human osteosarcoma cells In other human cancers, the association of Bcl-xL expression and radiosensitivity of tumor cells is also ... **P

Ngày tải lên: 09/08/2014, 02:21

... transcripts Expression of BMP-2 and chordinstem cells during osteogenic differentiation of human mesenchymal stem cells (a) Temporal progression of bone morphogenetic protein (BMP)-2 and chordin expression ... Lecanda F, Avioli LV, Cheng SL: Regulation of bone matrix protein expression and induction of differentiation of human osteoblasts and human bone marrow stromal cells by bone morphogenetic protein-2 ... performed using WST-1 assay at 3, and days of culture After and days, cells treated with chordin siRNA had decreased proliferation, as compared with untreated cells, and cells treated with transfection...

Ngày tải lên: 09/08/2014, 10:23

... expression and to induce differentiation of monocytes into dendritic cells (DCs) [19-26], antigen-presenting cells that induce and regulate immune responses An increased number of circulating plasma cells ... CD4+ T cells/ well, at ratios of DCLCs to T cells of 1:10, 1:20 and 1:40 Three days later, [3H]thymidine was added (0.5 μCi/well) and the cells were incubated for another 18 hours The cells were ... Riedel D, Herrmann F: Induction of monocytic differentiation and modulation of the expression of c-fos, c-fms and c-myc protooncogenes in human monoblasts by cytokines and phorbolester Virchows Arch...

Ngày tải lên: 09/08/2014, 10:23