generation and detection of dual tone multifrequency tones using goertzel algorithm
Báo cáo khoa học: Identification of a preferred substrate peptide for transglutaminase 3 and detection of in situ activity in skin and hair follicles pdf
Ngày tải lên :
29/03/2014, 21:20
... localization of activated TGase 3, which was found to display a pattern distinct from that of TGase 1, was observed by the detection of in situ activities using this peptide Results Screening of candidate ... activity in both human and mouse skin [16,19] These studies have provided new insight into the substrate specificity of Tgases and have expanded the range of application of the enzyme reaction ... the precise role of this isozyme in a variety of tissues and cells Recently, detection of altered enzymatic activities in patients with TGase mutation was successfully achieved using FITC–pepK5...
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Báo cáo toán học: "On the generation and enumeration of some classes of convex polyominoes" pps
Ngày tải lên :
07/08/2014, 08:20
... technique of choice is the ECO method, for a survey of which we refer to [4] The crux of this method is the recursive generation of classes of combinatorial objects through local expansions of the ... using MAPLE to give g(x) as in (4) In latter part of the paper we examine the exhaustive generation of the classes of convex and column-convex polyominoes The aim in studying the exhaustive generation ... occurrence of (1)a with (1)b Proof Our first step is to compute the equations for A and B Figure 17 shows a rough decomposition of the generating trees of Ω and Ω The equation for A Let w be a word of...
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Báo cáo khoa học: "Isolation and identification of Escherichia coli O157:H7 using different detection methods and molecular determination by multiplex PCR and RAPD" docx
Ngày tải lên :
07/08/2014, 18:21
... strains), C1-6 (strains of Cornell Univ.) and P1-7 (strains from E coli reference center of Pennsylvania State Univ.) b The presence of Stx1 and Stx2 “-” and “+” indicate negative and positive, respectively ... Switzerland) Once suspicious colonies are identified, confirmation of the isolates as E coli O157:H7 is dependent upon biochemical identification and demonstration of the presence of somatic and ... was collected and used as a template Multiplex PCR for stx1, stx2, eaeA, and hlyA, and the flagellar H7 gene amplification Multiplex PCR for the detection of stx1, stx2, eaeA, and EHEC hlyA gene...
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Báo cáo y học: " Generation and characterization of high affinity human monoclonal antibodies that neutralize staphylococcal enterotoxin B" ppsx
Ngày tải lên :
11/08/2014, 08:21
... regulations and guidelines of the National Institutes of Health (NIH) and with the approval of the TPPS Animal Care and Use Committee Mice were challenged with various concentrations of SEB (Toxin ... concentration of 10 μg/ml and coupled to the surface of a research-grade CM5 chip (Biacore, Inc., Piscataway, NJ) by standard amine chemistry (NHS-EDC, Biacore, Inc.), to a level of 50-250 RU of SEB ... quenched using M ethanolamine A reference flow cell consisting of an activated and quenched surface in the absence of antigen was created and used to normalize readings from injections of anti-SEB-containing...
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báo cáo khoa học: " Characterization of PR-10 genes from eight Betula species and detection of Bet v 1 isoforms in birch pollen" pot
Ngày tải lên :
12/08/2014, 03:20
... presence and relative abundances of individual isoforms The pollen of four birch species contained a mixture of Bet v isoforms, with abundant levels of isoforms that were similar to isoforms with ... for isoform 02C01 and comprises 17% of all fragment IIIvariants, while peptides IIIb and Xb are unique for 01B01 and comprise 1819% of all fragment III and X-variants The isoforms 02A01 and 02B01 ... mixture of multiple isoforms and their posttranslational modifications, which could not be separate completely by 2-Dimension gel electrophoresis A disadvantage of using Q-TOF LC-MSE instead of Q-TOF...
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Báo cáo y học: " CD39+ Regulatory T cells suppress generation and differentiation of Th17 cells in human malignant pleural effusion via a LAP-dependent mechanism" pps
Ngày tải lên :
12/08/2014, 13:22
... cytokines that reported be involved in generation and differentiation of Tregs or Th17 cells, and observed that high levels of IL-1b, IL-6, and TGF-b1, but not of IL-23, in MPE when compared with ... cytokines might affect the generation and differentiation of Tregs or /and Th17 cells in MPE To evaluate the contribution of cytokines to the numbers of pleural CD39+Tregs and Th17 cells, we purified ... combinations of IL-1b, IL-6, and IL-23 did not affect the increase in CD39+Treg numbers induced by TGF-b (Figure 4) Inhibition of Generation and Differentiation of Th17 Cells by CD39+Tregs On the basis of...
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Báo cáo y học: "Activation and detection of HTLV-I Tax-specific CTLs by Epitope expressing Single-Chain Trimers of MHC Class I in a rat model" pptx
Ngày tải lên :
13/08/2014, 05:21
... CTLs and HTLV-I infected T cells We have previously established a rat model of ATL-like disease, which allows examination of the growth and spread of HTLV-I infected cells, as well assessment of ... Establishment of MOLT-4 cells stably expressing SCTs of RT1.Al To examine the effects of rat SCTs expressed on human cells and the influence of linker length on the activation of CTLs in more ... suggested the importance of Tax-specific CTLs for prevention and therapy of ATL and should be further verified using suitable animal models Rats have been used for a number of studies on HTLV-I infection,...
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Báo cáo sinh học: "Analysis of a simulated microarray dataset: Comparison of methods for data normalisation and detection of differential expression (Open Access publication)" pps
Ngày tải lên :
14/08/2014, 13:22
... (gene), mean and standard deviation of the unnormalised log ratio (M and SD), mean and standard deviation of the LOESS normalised log ratio (M LOESS and SD LOESS) and the number of methods in ... id (gene), mean and standard deviation of the unnormalised log ratio (M and SD), mean and standard deviation of the LOESS normalised log ratio (M LOESS and SD LOESS), the number of methods in which ... simulation software Array number of grid rows Array number of grid columns Number of spots in a grid row Number of spots in a grid column Number of spot pins Number of technical replicates Number of genes...
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Báo cáo y học: "Segmentation-based detection of allelic imbalance and loss-of-heterozygosity in cancer cells using whole genome SNP arrays" ppsx
Ngày tải lên :
14/08/2014, 20:22
... on the basis of their segmented mBAF values Evaluation and comparison of sensitivity and specificity using simulated Illumina data To investigate the sensitivity and specificity of the segmentation ... mBAF threshold of 0.97 and a triplet sum threshold of 0.8 for Illumina data For Affymetrix data, we used an mBAF threshold of 0.9 and a triplet sum threshold of 0.6 Segmentation of allelic proportions ... gain, below -0.14 as loss, and in between as copy neutral Analysis of and Generation experimental data Results using Click here data analysis of the the approach fileof 3filtering simulated the...
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GENERATION AND CHARACTERIZATION OF HUMAN MONOCLONAL ANTIBODIES WITH NEUTRALIZING ACTIVITY FOR DENGUE VIRUS
Ngày tải lên :
09/09/2015, 08:16
... biology and Conrad and Dominik for the initial generation of 10.15 To Dr Lok Shee-Mei, Petra and Jiaqi, thank you for solving the cryo-EM structure of 14C10 and 10.15 To our collaborators at NUH and ... Figure 23 Binding activity of 10.15 to various strains of DENV2 and DENV1, and 101 Figure 24 Binding activity of 12.17 to various strains of DENV2 and DENV1, and 102 ... Philippines and recent isolates from South Pacific islands Thailand, Vietnam and Bangladesh Sri Lanka, India, Africa, Samoa and 1962 strain from Thailand Puerto Rico, Latin and central America and 1965...
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Entangled photon pairs efficient generation and detection, and bit commitment
Ngày tải lên :
09/09/2015, 11:19
... temperature T of the crystal The wavevectors kp , ks and ki are functions of ωp , ωs and ωi and the refractive indices (np , ns and ni ) of the medium, which in turn are a function of the optical ... certain coincidence time window (τc ) of each other, then these photons are considered part of a pair Given the rate of pairs (p) and the rate of individual detection events from each detector ... system efficiency of 75.2 % The measurement was taken for 30 s and we used a coincidence time window (τc ) of 800 ns We observe a pair rate of 13366.3 /s and singles rates of 19477.2 /s and 16646.0...
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Generation and analysis of KRAS v12 in driving liver tumorigenesis using transgenic zebrafish models
Ngày tải lên :
10/09/2015, 15:48
... mimics human diseases and offers the ability to quickly and inexpensively test the efficacy and safety of compound libraries (Amatruda and Patton, 2008; Liu and Leach, 2011; Zon and Peterson, 2005) ... transgenesis and microinjection, further strengthen the use of zebrafish Recently, there is increased generation and analysis of zebrafish models of human diseases (Amatruda and Patton, 2008; Liu and ... mifepristone-inducible LexPR system (Emelyanov and Parinov, 2008) Emelyanov and Parinov demonstrated that one of the utilities of the mifepristone-inducible system is the generation of two independent...
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Generation and control of quantum entanglement in physical systems
Ngày tải lên :
10/09/2015, 15:48
... roots of the moduli of the eigenvalues of ρ.˜ ρ y y y y with ρ = (σ1 ⊗ σ2 ).ρ∗ (σ1 ⊗ σ2 ) and ρ∗ is the complex conjugate of ρ As EF ˜ is a monotonically increasing function of C, and the ranges of ... four topics in the field of generation and control of quantum entanglement in physical systems The first topic concerns the maximal entanglement generation in a spin chain of XX model with specially ... [5] Li Dai and Leong Chuan Kwek Generation of boundary entanglement in a chain of coupled double quantum dots, accepted to be published in Laser Physics ix List of Figures 1.1 The set of zero-discord...
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Isolation, purification and detection of soyasaponins and their associated bioactivities in cultured hepatocarcinoma cells
Ngày tải lên :
11/09/2015, 10:06
... Structures and molecular weights of soyasapogenol B, C and B1 98 Fig 28 LC-MS Chromatogram of standard and purified sample of soyasapogenol B Panels (A and C) are authentic standard and samples of ... 79 6.2.1.5 HPLC Analysis of Soyasaponins and Soy isoflavones 79 6.2.1.6 LC/MS Analysis of Total soyasaponins and Soy isoflavones 79 6.2.2 Comparison of Soyasaponin Concentration Methods ... recovery of soyasapogenols A and B without producing artifacts (Ireland and Dziedzic 1986; Rupasinghe, Jackson et al 2003) Moreover, Ireland and Dziedzic (Ireland and Dziedzic 1986) showed that...
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Analysis and detection of human motion in time frequency domain
Ngày tải lên :
14/09/2015, 18:27
... decomposition and reconstruction process, we still need to understand the nature of the frequency bandwidth of decomposed and reconstructed signals Table 3.1 shows the frequency bandwidths of decomposed and ... coefficients and 93 separated segments of human motion patterns in 12 the acceleration signals 4.20 44 pairs of Pa and Pv for ascending and descending stairs 96 and 66 pairs of Pa and Pv for level ... mean and one side standard deviation of positive peak 122-124 and negative peak angular rates of gyroscope sensors at position FW, S and RU Both positive and negative peak angular rates of fall...
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Linearization and control of dual axis micromirror
Ngày tải lên :
15/09/2015, 17:10
... LINEARIZATION AND CONTROL OF DUAL- AXIS MICROMIRROR ZHAO YI (M Eng.) A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY DEPARTMENT OF MECHANICAL ENGINEERING NATIONAL UNIVERSITY OF SINGAPORE ... [14]-[16] and endoscopic optical coherence tomography [17] This device is generally a double-gimballed structure and has 3-DOF (degrees of freedom), two rotation motions (around x -axis and y-axis) and ... details of all the aforementioned methods 1.5 Purpose and Scope of Thesis As mentioned in Section 1.4, the performance of the dual- axis micromirror is greatly limited due to operation nonlinearity and...
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Analysis and detection of human emotion and stress from speech signals
Ngày tải lên :
15/09/2015, 22:03
... neighbours of winning neuron fi center frequency of i subband bi bandwidth of i subband α logarithmic growth factor C bandwidth of first filter lm lower edge of m th filter bank hm upper edge of m ... Kyi Tar Nwe and other family members for their support, understanding and encouragement i Table of Contents Acknowledgements i Table of Contents ii Summary vi List of Symbols viii List of Figures ... Motivation of This Research 1.5 System Overview 1.6 Purpose and Contribution of This Thesis 10 1.7 Organization of Thesis 11 Chapter 2: Review of Acoustic Characteristics and Classification Systems of...
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Electrochemical generation and processing of porous silicon
Ngày tải lên :
05/10/2015, 19:03
... room temperature of the red band are of the order of several microseconds, and the red band is therefore also termed the slow-band (Sband) photolumincesence The green-blue band peak intensity ... non-parabolicity of the CB, the detailed shape of the VB, nor the influence of neighbouring bands A review of the using more sophisticated models that have been used to calculate of Econfined by ... introduction and literature survey of work done in both theoretical and experimental aspects of porous silicon and its micromaching technology followed by a description of the experimental methods and...
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Isolation and detection of bacteria in soil, ash and sediment with the contamination of paraquat and glyphosate
Ngày tải lên :
28/04/2016, 13:40
... one of the most powerful method based on using the ability of DNA polymerase to synthesize new strand of DNA complementary to the offered template strand and then amplify thousands to millions of ... DNA Ladder was added and mixed 20 µl of products of PCR and µl of 6x Loading Dye were put into gel which contained 0.5 x TPE buffer to prepare 1.0% agarose gel and µL of EtBr And the electrophoresis ... fingerprints; and the detection and diagnosis of hazardous diseases etc (Bartlett and Stirling, 2003) Particularly, PCR is commonly used in selective DNA isolation and detection of bacteria or viruses...
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Modelling and verification of single slope solar still using ANSYS-CFX
Ngày tải lên :
05/09/2013, 15:28
... cover made of toughed glass of mm is fixed to the top of vertical walls of the stills using a rubber gasket on both sides of glass and clamp fixed iron frames made of angles A plastic pipe is ... vertical side of still was kept as 0.30 m, whereas the height of higher vertical side was kept 0.85 m The effective basin area of meter x meter made of GRP of m thickness Condensing cover made of toughed ... Simulation result and experimental data In solar still, after evaporation, condensation process starts, and condensation produced by inner glass cover, and difference of temperature of water and glass...
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