general protocol for agrobacterium mediated transformation

... the control (e) 126 Developing an Agrobacterium- mediated transformation system It is obvious that transformation for lilies is still difficult with very low transformation efficiency Efficient ... expression, implying a successful transformation of Lilium x formolongo by Agrobacterium using thin cell layers of bulb scales Fig Results of transformation via Agrobacterium using thin cell layers ... transgenic plants via Agrobacterium- mediated transformation in the Liliaceous ornamentals was affected by several factors such as, target material for Agrobacterium inoculation, kind of Agrobacterium...

Ngày tải lên: 06/08/2014, 19:20

... Matsuoka, M A protocol for Agrobacteriummediated transformation in rice Nat Protoc 1, 2796–2802 (2006) 18 Zhang, X.R., Henriques, R., Lin, S.S., Niu, Q.W & Chua, N.H Agrobacterium- mediated transformation ... shaker (~50 r.p.m.) for 45 at room temperature 1850 | VOL.4 NO.12 | 2009 | nature protocols protocol Agrobacterium- mediated transformation of FEC ● TIMING d 16| Pipette the Agrobacterium suspension ... provides an indication of their transformation viability and ultimately can be regenerated for use as nontransgenic control plantlets Phase III: Agrobacterium- mediated transformation After ~14 weeks,...

Ngày tải lên: 31/05/2014, 09:26

... in Agrobacterium- mediated plant transformation Molecular and General Genetics, 220: 245–250 Wenck A.R., Quinn M., Whetten R.W., Pullman G., Sederoff R (1999): High-efficiency Agrobacteriummediated ... Stable transformation of Picea-glauca by particle-acceleration Bio-Technology, 11: 84–89 Fillatti J.J., Sellmer J., McCown B., Haissing B., Comai L (1987): Agrobacterium mediated transformation ... radiata after Agrobacterium tumefaciens -mediated transformation using nurse cultures Plant Cell Reports, 23: 606–616 Charest P.J., Devantier Y., Lachance D (1996): Stable genetic transformation of...

Ngày tải lên: 07/08/2014, 10:21

... Transformation 46 2.3.1 Transformation of E coli by heat shock 46 2.3.2 Transformation of S cerevisiae by lithium acetate transformation 46 2.3.3 Agrobacterium- mediated transformation ... regulate the Agrobacterium- mediated transformation (AMT) process partly through this chromatin remodeling complex since knockout of the other subunits of the complex also increases transformation ... effector in regulating Agrobacterium- mediated transformation (In preparation) Chen Z and Pan S.Q (2012) T-DNA detection and trafficking in the yeast cell during Agrobacterium- mediated gene transfer...

Ngày tải lên: 09/09/2015, 10:08

... BioMax XAR films with an X-ray developer 2.5 Agrobacterium- mediated transformation of yeast Agrobacterium- mediated transformation of yeast was performed similarly as described with a few modifications ... Agrobacterium- mediated transformation 81 4.2.2 Yeast Pmp3p is a membrane protein related to cellular ion homeostasis 82 4.2.3 Resistance of pmp3∆ to Agrobacterium- mediated transformation ... Study of host Pmp3p in Agrobacterium- mediated transformation of yeast 81 4.1 Introduction 81 4.2 A host Pmp3p affected Agrobacterium- mediated transformation in yeast...

Ngày tải lên: 09/09/2015, 11:19

... 63 4.1 Introduction 63 4.2 Agrobacterium- mediated transformation in different K+ levels 63 4.3 Agrobacterium- mediated transformation under NaCl stress 67 4.4 Conclusions ... plant mutant is still a hard work for scientists Therefore, it is important for us to look for other methods and model organisms Using the Agrobacterium- mediated transformation, scientists have built ... factors to transformation process Many studies have identified host genes that affect Agrobacterium- mediated transformation (Gelvin et al., 2000) Therefore, by evaluating the outcome (transformation...

Ngày tải lên: 16/10/2015, 11:57

... events in Agrobacterium- mediated transformation of Aspergillus awamori Curr Genet 45:399-403 Michielse CB, Arentshorst M, Ran AFJ, Hondel CAMJJ van den (2005a) Agrobacterium- mediated transformation ... Agrobacteirum -mediated transformation as a tool for functional genomics in fungi Curr Genet 48:1-17 Michielse CB, Hooykaas PJJ, Hondel CAMJJ van den, Ran AFJ (2008) Agrobacterium- mediated 22 transformation ... the formation of the albino mutant, complementation tests were performed (Fig 3A) Transformation of the alb1 mutant with pAUR-PksP yielded transformants with black conidia, whereas all transformants...

Ngày tải lên: 20/06/2014, 21:20

... function investigation Figure 10 corniculatus for A rhizogenes -mediated transformation of L A flowchartcv Superroot A flowchart for A rhizogenes -mediated transformation of L corniculatus cv Superroot ... rhizogenes -mediated transformation was developed, combining the simplicity and efficiency of the Superroot regeneration system and the highly efficient A rhizogenes mediated transformation The transformation ... rhizogenesmediated transformation [10,11,38] and also A tumefaciens -mediated transformation [11,15,39], the standard procedure, which was used to optimize parameters influencing the transformation...

Ngày tải lên: 12/08/2014, 03:20

... Healthcare use Medication use in last month Consultation in last 12 months for foot pain For foot pain, for other pain General practitioner, physiotherapist, podiatrist, chiropodist (NHS and private) ... Right/left; forefoot, mid-foot, heel, ankle < year, 1- < years, 5- < 10 years, 10+ years ago Planned treatment Are you waiting for any appointments or treatments for Yes/no (free text comments for yes) ... Pre-defined protocols for all components of the interview and assessment will be used for standardisation between Research Assessors Assessment findings will be recorded on a standard form that...

Ngày tải lên: 10/08/2014, 21:24

... to as information security 2.2 Information Security Security is a property of a well-designed system according FISMA [10] The term information security means protecting information and information ... which they are running The platform is typically an application framework Generally speaking, PaaS service providers are responsible for the security of the platform software stack, and the recommendations ... considered The identity provided for that employee must be used for every purpose that warrants the use of ID Therefore, most of the existing IDM cannot be used for all situations The federated...

Ngày tải lên: 31/07/2013, 09:43

... limitation, selection and transformation Let me ®rst explain limitation and transformation Figure 10.3 illustrates the general abstract relation between limitation and transformation: structures ... ``weak'' class formations; unitary or fragmented class formations; revolutionary, counterrevolutionary or reformist class formations Typically, class formations involve creating formal organizations ... generally class struggles involve collectivities of various sorts Class formations, not atomized individuals, are the characteristic vehicles for class struggles Class struggles, therefore, generally...

Ngày tải lên: 01/11/2013, 07:20

... the basics to the art, from information to truth by following Paul’s teachings to speak from the heart Enjoy your public speaking transformation and, thank you for putting your faith into action ... speaking before your audience has finished listening." It is always a good idea to leave them wanting more So, finish early and leave extra time for Q&A LWR: Tips, Tools, and General Guidance for Public ... the content and/or format to best fit your public speaking goals and activities LWR: Tips, Tools, and General Guidance for Public Speaking, Page 34 Public Speaking Journal — Before Speaking Your...

Ngày tải lên: 20/12/2013, 23:15

... appropriate Index for it to be in Here are some general guidelines Standard & Poor’s uses for each of its Indices: For the S&P SmallCap 600, Standard & Poor’s generally looks for companies with ... First and foremost, S&P Indices are not rules-based; all changes are fully discretionary and are determined by the Index Committee based upon public information Companies may not apply for inclusion ... GENERAL CRITERIA FOR S&P U.S INDEX MEMBERSHIP INTRODUCTION One of the most frequent questions we get at Standard & Poor’s is, “What are the criteria for being added to an...

Ngày tải lên: 17/01/2014, 03:20

... 637–664 Weak mixing for interval exchange transformations and translation flows By Artur Avila and Giovanni Forni* Abstract We prove that a typical interval exchange transformation is either weakly ... definitions that every mixing transformation is weakly mixing and every weakly mixing transformation is ergodic A classical theorem states that any invertible measure-preserving transformation f is weakly ... holonomy For a general flat surface the cone angles at the singularities are 2π(κ1 + 1) ≤ · · · ≤ 2π(κr + 1), where κ1 , , κr > −1 are real numbers WEAK MIXING FOR INTERVAL EXCHANGE TRANSFORMATIONS...

Ngày tải lên: 16/02/2014, 06:20

... Word features: wi for i in {−2, −1, 0, +1, +2}, wi ∧ wi+1 for i in {−1, 0} • Tag features: wi for i in {−2, −1, 0, +1, +2}, ti ∧ ti+1 for i in {−2, −1, 0, +1} ti ∧ ti+1 ∧ ti+2 for i in {−2, −1, ... anonymous reviewers for useful discussion Thank you to Andriy Mnih for inducing his embeddings on RCV1 for us Joseph Turian and Yoshua Bengio acknowledge the following agencies for research funding ... This technique for turning a supervised approach into a semi-supervised one is general and task-agnostic However, we wish to find out if certain word representations are preferable for certain tasks...

Ngày tải lên: 20/02/2014, 04:20

... ASR transcripts for lectures Section describes our experimental setup, and Section analyses its results Transformation- Based Learning Brill’s tagger introduced the concept of Transformation- Based ... aligned with the same sentences tagged by the system Typically, rules take the form of context-dependent transformations, for example “change the tag from verb to noun if one of the two preceding ... adapted from the general- purpose algorithm introduced by Brill (1992) The transformation rules are contextual wordreplacement rules to be applied to ASR transcripts, and are learned by performing a...

Ngày tải lên: 20/02/2014, 07:20

... qualitative aspects, for instance wishes for more information The questionnaire contains the following scales: (a) Information about the disease; (b) Information about medical tests; (c) Information about ... (d) Information on other services, and single items: (a) Written information; (b) Information on CDs or tape/ video; (c) Satisfaction with the amount of information; (d) Desire for more information; ... formal document that also includes recommendations for subsequent cancer surveillance, management of late effects, and strategies for health promotion Essential in such an SCP is detailed information...

Ngày tải lên: 05/03/2014, 15:20

... 2004; and • the GHG Protocol for Project Accounting (this document) PA R T I CHAPTER 1: 1.1 The GHG Protocol for Project Accounting The GHG Protocol for Project Accounting (Project Protocol) provides ... establishment phase can also be large for some land-use projects For example, reforestation and afforestation projects often require the clearing of vegetation to prepare a site for planting This results ... should strive for maximum transparency when justifying the basis of such decisions and fulfilling the Project Protocol s requirements 1.3 Overview of the Project Protocol The Project Protocol has...

Ngày tải lên: 06/03/2014, 21:20

... regarding the format, style and general approach used for the audit protocols The last appendix in each protocol document contains a user satisfaction survcy and comment form This form is to be ... or before December 22 1988 or one for which installation commenced on or before December 22, 1998.) Verify that existing USTs meet one of the following standards: -the performance standards for' new ... any statement(s) contained herein - II Protocol for Conducting Environmental Compliance Audits o f Storage Tanks under RCRA The Audit Protocols are designed for use by individuals who are alreadv...

Ngày tải lên: 06/03/2014, 23:20