general chemical composition of crude oil

... relationship of all of these genes remains to be clarified Expression and purification of Sno1p and Snz1p In light of the similarity in the amino acid sequence of Sno1p to that of the glutaminase subunit of ... capable of growing in –PN medium Site of mutation in K64 In order to identify the site of mutation, the two genes were amplified by PCR with the chromosomal DNA of mutant K64 as template Sequencing of ... absorbance of the supernatant was read at 363 nm The absorbance of the reduced form of APAD (APADH) was linear over the 2–100 lM range of glutamate with a molar extinction coefficient of 8900 M)1Æcm)1...

Ngày tải lên: 19/02/2014, 12:20

... impression of confidentiality of web surfing An encrypted connection is not sufficient to remove traffic patterns that often reveal the website a user visits For example, size of the base HTML file of a webpage ... aggressiveness of applying the countermeasures and the willingness to trade off the overheads impact the effectiveness of the anonymity protection List of Tables 3.1 Components of Source Identification ... component lengths False positives of AMS and RPM (noiseless) False positives of AMS and RPM (noisy) False positives of AMS and RPM by distance False positives of RPM 58 60...

Ngày tải lên: 11/09/2015, 09:58

... accomplished by varying the size of the solenoid coils, thickness of iron jacket, and amp-turns (NI) The optimum values of NI for the side and middle solenoid coils were obtained to be 12950 and ... fabrication, and characterization of a solenoid system 463 Table The optimized design parameters for solenoid coil with iron jacket Description Solenoid coil Coil type Coil size φ300 mm × 80 mm Bore ... middle and side solenoid coils respectively Three solenoid coils were used to get; (i) mirror field, (ii) flat field, and (iii) off-resonance field configuration Two side solenoid coils, which were identical,...

Ngày tải lên: 22/12/2013, 08:58

... model is based on a corpus representative of the web In this implementation of the model we approximated one of the possible compositions of a random slice of the web, statistically supported by ... mixture of Biberian features and additional syntactic traits The total number of features used in this implementation of the model is 100 These features are available online at: text types> of http://www.nltg.brighton.ac.uk/home/Marina.Santini/ ... implement For this reason, the focus of the method proposed below is not to detect emerging genres, but to show a flexible approach capable of giving account of genre hybridism and individualization...

Ngày tải lên: 23/03/2014, 18:20

... provided The use of the CODEHOP methodology for the analysis of larger regions of viral genomes is presented along with the general application of this technology for the identification of viral species ... genomes Often the linear order of genes within the genomes of related viruses is maintained Thus, the spacing and orientation of specific genes can be predicted in the genomes of Page 15 of 24 ... Figure KSHV of a region12 the divergent locus B of a complete sequence CODEHOP strategy to determine themacaque homolog ofof CODEHOP strategy to determine the complete sequence of a region of the...

Ngày tải lên: 18/06/2014, 22:20

... data represent the average of three independent sets of experiments with each set of experiments performed twice D Structural classification of compounds A series of compounds that independently ... Argonne National Laboratory is operated by the University of Chicago Argonne, LLC, for the United States Department of Energy Office of Biological and Environmental Research under Contract DE–AC02–06CH11357 ... chemoresistance Thomas D Hurley Ph.D., Chair vii Table of Contents List of tables xi List of figures xii List of abbreviations xiv I Introduction ...

Ngày tải lên: 24/08/2014, 09:51

Ngày tải lên: 14/11/2014, 06:20

... position of the footprint is read of the gel in relation to the labeled end of the probe The advantage of this method is that single base pair resolution of binding sites can be obtained One of the ... controlling the specificity of gene expression Promoters generally direct basal level of gene transcription, but both the amount of transcript and subtle changes in expression domains of a gene is controlled ... that of protein coding genes They generally consists of a single or a group of transcription factor binding sites arranged within them and there is very limited knowledge on the structure of the...

Ngày tải lên: 14/09/2015, 08:40

... THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY DEPARTMENT OF MEDICINE YONG LOO LIN SCHOOL OF MEDICINE NATIONAL UNIVERSITY OF SINGAPORE 2007 ACKNOWLEDGEMENT First of all, I would like to express ... all staff of Deparment of Rheumatology and Immunology, Singapore General Hospital for their help and friendship ii Thanks to all staff of Department of Clinical Research, Singapore General Hospital, ... appreciation to my supervisor Associate Professor Fong Kok Yong, Chairman of Division of Medicine, senior consultant, Department of Rheumatology and Immunology in Singapore General Hospital, for his invaluable...

Ngày tải lên: 14/09/2015, 12:42

... advantages of baculovirus include the ease of construction of recombinant viral vector, simple procedure for purification of large volumes of high titre viruses and the virtual absence of cytotoxicity ... promoter is that of the survivin gene Survivin is a member of the Inhibitor of Apoptosis (IAP) protein family and plays a vital role in the survival of cancer cells and the progression of malignancy ... al., 1998) Another advantage of restricted expression of the suicide gene is the use of general delivery systems 7    Retroviruses are used for tumour-specific delivery of therapeutic genes as they...

Ngày tải lên: 22/10/2015, 21:20

... total amount of pravastatin entrapped in 0.1 ml of the final packed erythrocytes Efficiency of entrapment The percentage of the loaded amount of pravastatin to the total amount of that added ... ml eppendorff tubes, 400 µl of suspension are added to 400 µl of PBS containing the known concentration of the drug and 2.5 mmol of ATP, 2.5 mmol MgCl2 and 2.5 mmol of CaCl2, gently mixing to ... against lysis as a result of the osmotic pressure changes of their surrounding media was evaluated Twenty five l of erythrocyte sample was added to each of a series of 2.5 ml saline solutions...

Ngày tải lên: 25/10/2012, 11:10

... onset of the first major weight loss at about 520 K corresponds to the decomposition of the PS polymer component The second major weight loss commences at about 710 K, corresponding to the decomposition ... International Journal of Energy and Environment (IJEE), Volume 1, Issue 4, 2010, pp.643-656 647 3.3 XPS analysis of the PVDF-SPS composite membrane The surface compositions of the PVDF-SPS composite ... hydrophobic property of PS polymer promoted the formation of these polymer aggregates As can be seen, the thermally induced polymerization of styrene, as well as the blending of PS and SPS polymers,...

Ngày tải lên: 05/09/2013, 16:11

... of organic acid and reduced levels of ammonia after compost processing [41, 42] Table Characterization of yard waste compost samples Specifications YWCa-I YWC-II Location Bottom of heap Top of ... Hackensack, NJ) During extrusion, a screw speed of the extruder of 100 rpm and a barrel temperature of 100°C was maintained [28] 2.3 Sample collection Samples of yard waste compost (YWC) and finished ... unit (U) of enzyme activity was expressed as the amount of enzyme liberating µM of glucose (cellulase) or xylose (xylanase) equivalents per under the assay conditions 2.7 Morphology of microbial...

Ngày tải lên: 05/09/2013, 16:11

... repertoire of applications can only be expected to grow in the future through the development of specific chemical or enzymatic sorting reactions that alter the chemical nature of a predetermined set of ... the actual site of proteolytic cleavage, data interpretation and the design of followup analyses are straightforward COFRADIC-based sorting of N-glycosylated peptides Glycosylation of asparagines ... proteomics techniques [72], COFRADIC has a number of unique properties As COFRADIC is essentially a peptide-sorting technology by which only a set of peptides representative of the proteomic problem...

Ngày tải lên: 18/02/2014, 16:20

... unknown but in view of the extensive differences between the TP isoforms in terms of their mechanisms of signaling [18,19], modes of regulation ⁄ desensitization [20–23] and patterns of expression ... that disruption of both sites obliterates basal Prm3 activity Investigation of the role of the AP-1 site at -27 in phorbol myristic acid (PMA) induction of promoter Fig Demonstration of nuclear factor ... It is anticipated that the functional characterization of Prm3 reported herein should provide critical knowledge of the modes of regulation of TPb expression and hence may shed further insights...

Ngày tải lên: 19/02/2014, 16:20

... CCAAT box at 324 bp upstream of the major transcription start site The presence of a CCAAT box and the absence of a TATA box seem to be one of the characteristics of the mouse integrin a3 gene ... approximately 4.0 kb of the 5¢-flanking region of exon of the mouse integrin a3 subunit gene We prepared a chimeric construct (pGL-ES), in which the 4.0 kb EcoRI/SacI fragment upstream of exon was inserted ... 5¢-flanking region and determination of transcription start sites of mouse integrin a3 subunit gene The nucleotide sequence of the 0.5 kb SalI/SacI fragment and a part of exon is shown in Fig A TRANSFACTM...

Ngày tải lên: 21/02/2014, 03:20

... degree -of- fold, Q, is responsible for high yield stress of biological protein materials through breakage of hydrogen bond of β-sheet structural motif For deeper understanding the role of native ... toughness better than that of Kevlar.6 This highlights that understanding of mechanical behavior of protein materials such as spider silk may provide the key concept for design of biomimetic materials, ... a given position vector r of a protein structure is in the form of u ( r ) = Δε ⋅ r (1) Accordingly, the position vector r* of a protein structure after application of discrete, constant strain...

Ngày tải lên: 22/02/2014, 08:20

... low amount of the C14 dialdehyde is probably a result of instability The formation of b-ionone from b-apo-8¢-carotenal (C30) and b-apo10¢-carotenal (C27) suggested that the cleavage of the C9–C10 ... cleavage of apo-10¢-lycopenal (C27; substrate II) led to a more complex mixture of products, including three compounds (products 1, and 5) identified as dialdehydes on the basis of the fine structure of ... detection of geranial (C10) produced from 3-OH-c-carotene in vitro A B Fig Determination of the relative amounts of dialdehyde products (A) Incubations with apo-8¢-lycopenal (C30) The peak areas of...

Ngày tải lên: 07/03/2014, 03:20