... relationship of all of these genes remains to be clarified Expression and purification of Sno1p and Snz1p In light of the similarity in the amino acid sequence of Sno1p to that of the glutaminase subunit of ... chloroform-t-amyl alcohol-glacial acetic acid (70 : 30 : 3, v/v/v) The dansylated products were visualized under ultraviolet light Inhibition of Sno1p with DON Inhibition of the complex of Sno1p ... capable of growing in –PN medium Site of mutation in K64 In order to identify the site of mutation, the two genes were amplified by PCR with the chromosomal DNA of mutant K64 as template Sequencing of...
... packet features and possibly featuresof web objects Flow logs preserve features and statistics of flows and possibly object features, too Identifying subnet structures and key servers of an organization ... traffic analysis are generally size, timing and structure related These features can be properties of packets, file objects, or protocols Packet features are less stable than featuresof file objects, ... reveal featuresof the source data For example, to trace the attack paths of a flooding DDoS attack, controlled flooding exploits the fact that the packet rate of one flow affects the packet rates of...
... by the ECR community for the extraction of singly charged ion beams The ‘2D Poisson’ software package has been used in the present work for simulation of the magnetic field pattern using solenoid ... consumption The use of jacket is to provide a return path for the magnetic field lines and thereby shield the adjacent components The use of electromagnet has wide flexibility of tuning the plasma ... conditions The use of electromagnet (instead of permanent magnets) helps one to investigate the plasma parameters to optimize the beam current A conventional ECR ion source uses the principle of adiabatic...
... model is based on a corpus representative of the web In this implementation of the model we approximated one of the possible compositions of a random slice of the web, statistically supported by ... additional syntactic traits The total number offeatures used in this implementation of the model is 100 These features are available online at: text types> of http://www.nltg.brighton.ac.uk/home/Marina.Santini/ ... in terms of genre evolution in future investigations Attributes of the Tuple The attributes
... latest refined assays and their utility are provided The use of the CODEHOP methodology for the analysis of larger regions of viral genomes is presented along with the general application of this ... genomes Often the linear order of genes within the genomes of related viruses is maintained Thus, the spacing and orientation of specific genes can be predicted in the genomes of Page 15 of 24 ... Figure KSHV of a region12 the divergent locus B of a complete sequence CODEHOP strategy to determine themacaque homolog ofof CODEHOP strategy to determine the complete sequence of a region of the...
... generation of lipid peroxidation products ALDH3A1 can detoxify the productsof lipid peroxidation and hence facilitate drug resistance In fact, a recent study has shown that ALDH3A1 is one of the ... data represent the average of three independent sets of experiments with each set of experiments performed twice D Structural classification of compounds A series of compounds that independently ... chemoresistance Thomas D Hurley Ph.D., Chair vii Table of Contents List of tables xi List of figures xii List of abbreviations xiv I Introduction ...
... position of the footprint is read of the gel in relation to the labeled end of the probe The advantage of this method is that single base pair resolution of binding sites can be obtained One of the ... controlling the specificity of gene expression Promoters generally direct basal level of gene transcription, but both the amount of transcript and subtle changes in expression domains of a gene is controlled ... that of protein coding genes They generally consists of a single or a group of transcription factor binding sites arranged within them and there is very limited knowledge on the structure of the...
... THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY DEPARTMENT OF MEDICINE YONG LOO LIN SCHOOL OF MEDICINE NATIONAL UNIVERSITY OF SINGAPORE 2007 ACKNOWLEDGEMENT First of all, I would like to express ... all staff of Deparment of Rheumatology and Immunology, Singapore General Hospital for their help and friendship ii Thanks to all staff of Department of Clinical Research, Singapore General Hospital, ... appreciation to my supervisor Associate Professor Fong Kok Yong, Chairman of Division of Medicine, senior consultant, Department of Rheumatology and Immunology in Singapore General Hospital, for his invaluable...
... advantages of baculovirus include the ease of construction of recombinant viral vector, simple procedure for purification of large volumes of high titre viruses and the virtual absence of cytotoxicity ... promoter is that of the survivin gene Survivin is a member of the Inhibitor of Apoptosis (IAP) protein family and plays a vital role in the survival of cancer cells and the progression of malignancy ... al., 1998) Another advantage of restricted expression of the suicide gene is the use ofgeneral delivery systems 7 Retroviruses are used for tumour-specific delivery of therapeutic genes as they...
... total amount of pravastatin entrapped in 0.1 ml of the final packed erythrocytes Efficiency of entrapment The percentage of the loaded amount of pravastatin to the total amount of that added ... ml eppendorff tubes, 400 µl of suspension are added to 400 µl of PBS containing the known concentration of the drug and 2.5 mmol of ATP, 2.5 mmol MgCl2 and 2.5 mmol of CaCl2, gently mixing to ... against lysis as a result of the osmotic pressure changes of their surrounding media was evaluated Twenty five l of erythrocyte sample was added to each of a series of 2.5 ml saline solutions...
... onset of the first major weight loss at about 520 K corresponds to the decomposition of the PS polymer component The second major weight loss commences at about 710 K, corresponding to the decomposition ... vibration modes of PVDF [28] The peak at 1200 cm-1 is found to be weak in the PVDF-based composite membranes Blending of styrene is confirmed by the presence of aromatic ring features at 1493 ... International Journal of Energy and Environment (IJEE), Volume 1, Issue 4, 2010, pp.643-656 647 3.3 XPS analysis of the PVDF-SPS composite membrane The surface compositions of the PVDF-SPS composite...
... of organic acid and reduced levels of ammonia after compost processing [41, 42] Table Characterization of yard waste compost samples Specifications YWCa-I YWC-II Location Bottom of heap Top of ... Engineering from University of Wisconsin, Madison, USA He has served as vice chair of the Biomass Energy and Industrial Products committee, and associate editor of Transactions of the ASABE Kasi’s research ... unit (U) of enzyme activity was expressed as the amount of enzyme liberating µM of glucose (cellulase) or xylose (xylanase) equivalents per under the assay conditions 2.7 Morphology of microbial...
... repertoire of applications can only be expected to grow in the future through the development of specific chemical or enzymatic sorting reactions that alter the chemical nature of a predetermined set of ... the actual site of proteolytic cleavage, data interpretation and the design of followup analyses are straightforward COFRADIC-based sorting of N-glycosylated peptides Glycosylation of asparagines ... aid in interpreting structural featuresof ATP-binding proteins As expected, the majority of FSBA-labeled Jurkat proteins were known binders of small nucleotides, cofactors, or DNA and RNA molecules...
... unknown but in view of the extensive differences between the TP isoforms in terms of their mechanisms of signaling [18,19], modes of regulation ⁄ desensitization [20–23] and patterns of expression ... that disruption of both sites obliterates basal Prm3 activity Investigation of the role of the AP-1 site at -27 in phorbol myristic acid (PMA) induction of promoter Fig Demonstration of nuclear factor ... It is anticipated that the functional characterization of Prm3 reported herein should provide critical knowledge of the modes of regulation of TPb expression and hence may shed further insights...
... the sets of primers listed in Table After the PCR products were digested with KpnI and SacI, the fragments were inserted into the KpnI/SacI site of Fig Structures of the 5¢-flanking region of mouse ... CCAAT box at 324 bp upstream of the major transcription start site The presence of a CCAAT box and the absence of a TATA box seem to be one of the characteristics of the mouse integrin a3 gene ... 5¢-flanking region and determination of transcription start sites of mouse integrin a3 subunit gene The nucleotide sequence of the 0.5 kb SalI/SacI fragment and a part of exon is shown in Fig A TRANSFACTM...
... degree -of- fold, Q, is responsible for high yield stress of biological protein materials through breakage of hydrogen bond of β-sheet structural motif For deeper understanding the role of native ... toughness better than that of Kevlar.6 This highlights that understanding of mechanical behavior of protein materials such as spider silk may provide the key concept for design of biomimetic materials, ... a given position vector r of a protein structure is in the form of u ( r ) = Δε ⋅ r (1) Accordingly, the position vector r* of a protein structure after application of discrete, constant strain...
... of the C9–C10 double bond (a) results in products and from I, products and from II, and products and from III The cleavage of (b) leads to product from II, and product from III The cleavage of ... cleavage of apo-10¢-lycopenal (C27; substrate II) led to a more complex mixture of products, including three compounds (products 1, and 5) identified as dialdehydes on the basis of the fine structure of ... detection of geranial (C10) produced from 3-OH-c-carotene in vitro A B Fig Determination of the relative amounts of dialdehyde products (A) Incubations with apo-8¢-lycopenal (C30) The peak areas of...
... specificity and enzymatic properties of USP7 As part of the characterization of USP7 biochemical properties, we have measured its kinetic parameters for the hydrolysis of ubiquitin C-terminal 7-amido-4-methylcoumarin ... at a molar ratio of 100 : in a final volume of 150 lL Reactions were stopped by addition of one volume of · Laemmli sample buffer, followed by boiling at 95 °C for The effect of ubiquitin on the ... length), mg (1–560) and mg (208–1102) of purified recombinant protein per litre of insect cell culture In addition, an average of mg USP7 208-560 per litre of Escherichia coli fermentation broth...