gene therapy to enhance contractility

Báo cáo y học: "Gene Therapy: The Potential Applicability of Gene Transfer Technology to the Human Germline"

... issues related to human germline gene therapy Criteria for Assessing Applicability to Human Germline Gene Therapy An ideal gene transfer system in the context of human germline gene therapy would ... germline gene therapy, one would have to question whether our basic scientific understanding of retroviruses is sufficiently advanced to empower rational vector design Somatic gene therapy provides ... into hidden factors has been the notion of the existence of ‘inhibitory’ factors (IFs) associated with sperm cells These IFs are envisaged to prevent exogenous DNA uptake so as to protect the genetic...

Ngày tải lên: 03/11/2012, 10:01

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adenoviruses. basic biology to gene therapy

... Biology to Gene Therapy, edited by Prem Seth ©1999 R.G Landes Company 2 Adenoviruses: Basic Biology to Gene Therapy not have to wait until then,” and he telephoned Dr Rowe and told him to send ... ISBN 1-57059-584-4(alk paper) Adenoviruses Genetic vectors Gene therapy I Seth, Prem, II Series [DNLM: Adenoviridae Gene Therapy methods Genetic Vectors QW 165.5.A3 A2323 1999] QR396.A343 1999 ... vectors for gene therapy This book was written precisely to fulfill such a need The book is divided into six sections to review all the essential topics The first section describes the historic...

Ngày tải lên: 10/04/2014, 10:58

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Báo cáo y học: "Gene therapy of the rheumatic diseases: 1998 to 2008" ppt

... the risk -to- benefit ratio is more favorable toward gene therapy Lupus is thought to involve excess production of type cytokines, so a number of investigators have introduced type cytokines, such ... investigators have used adenovirus to deliver the immunoinhibitory receptor PD-L1, TACI (transmembrane activator and CAML [calcium modulator and cyclophilin ligand] interactor, an inhibitor of ... Thus, there is interest in a more general approach to therapy in which a transgene is introduced into a site where a secreted gene product will have access to the systemic circulation (Figure...

Ngày tải lên: 09/08/2014, 01:22

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An Introduction to Molecular Medicine and Gene Therapy - part 3 ppt

... prior to use Use of AAV Vectors for Gene Therapy A major advantageous characteristic of AAV vectors is their ability to transduce nondividing cells AAV vectors have been used to transfer genes into ... prior to use in humans Use of Adenoviral Vectors for Gene Therapy Adenoviral vectors have been used to transfer genes in vivo into the lung, liver, muscle, blood vessel, synovium, eye, peritoneum, ... the genetic material to be transferred 77 78 VECTORS OF GENE THERAPY VIRAL VECTORS USED FOR GENE THERAPY Based on the virus life cycle, infectious virions are very efﬁcient at transferring genetic...

Ngày tải lên: 10/08/2014, 14:22

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An Introduction to Molecular Medicine and Gene Therapy - part 4 ppsx

... HEMATOPOIETIC STEM AND PROGENITOR CELLS AS TARGETS FOR GENE THERAPY 143 144 GENE THERAPY FOR HEMATOLOGICAL DISORDERS restoration of cellular and humoral immunity were documented after gene therapy ... be used in gene transfer With regard to gene targeting, liposomes represent an important option To transfer foreign genes into a cell using a viral vector, the gene must be inserted into the viral ... common toxicities of chemotherapy regimens One approach to increase the tolerated dose of chemotherapy is to introduce the human multidrug resistance (MDR1) gene into bone marrow stem and progenitor...

Ngày tải lên: 10/08/2014, 14:22

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An Introduction to Molecular Medicine and Gene Therapy - part 6 ppsx

... neuroblastoma cells all modiﬁed to produce NGF In addition to gene therapy with neurotrophic factors, strategies that use regulatory proteins of cell death have been examined Antiapoptotic factors ... degeneration disorders remains to be elucidated Gene Therapy in Animal Models of Neural Degeneration At the present time CNS gene therapy initiatives follow in vivo and ex vivo approaches Gene ... of enhancing plasmid gene delivery Gene therapy approaches using either cytostatic, in which cells are prevented from progressing through the cell cycle to mitosis, or cytotoxic, in which cell...

Ngày tải lên: 10/08/2014, 14:22

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An Introduction to Molecular Medicine and Gene Therapy - part 7 pdf

... research on cytokines and cancer to use gene therapy to deliver cytokine gene( s) to tumor cells, thus creating an environment around the cell that would help to facilitate its destruction To date, ... sensitivity to antineoplatic drugs The protocols include both ex vivo and in vivo gene therapy techniques for cytokine or accessory molecule gene transfer, the gene transfer of prodrug-induced cytotoxicity, ... amenable to treatment by gene therapy approaches to inhibit the replication of HIV-1 The ultimate goal of gene therapy is to inhibit HIV-1 viral replication and the resulting AIDS pathogenesis For gene...

Ngày tải lên: 10/08/2014, 14:22

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An Introduction to Molecular Medicine and Gene Therapy - part 10 pot

... protocols prior to initiation of study, 309 Gene therapy vectors, gene transfer, 16 Genetic counseling, 348 Genetic diseases, 2–4 Genetic disorders: monogenetic, 4–5 multifactorial, 5–7 379 Genetic ... 147–149 Hematopoietic cells as gene therapy target, 133 Hematopoietic lineages, long-term genetic modiﬁcation, 140 Hematopoietic stem cells: as gene therapy targets, 137–145 HIV gene therapy, 280–281 ... International oversight, gene therapy research, 315 Intrabodies, 272 In vivo gene therapy, 12 I-SceI, gene targeting, 124–125 Ischemia, gene therapy, 196–198 jun D, gene transfer, rheumatoid arthritis,...

Ngày tải lên: 10/08/2014, 14:22

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Báo cáo khoa học: "Stable replication of the EBNA1/OriP-mediated baculovirus vector and its application to anti-HCV gene therapy" pot

... candidate vectors for in vitro gene therapy studies [14,15] These recombinant vectors contain compatible promoters and are highly effective in infecting primary hepatocyte and hepatoma cell lines, ... transgene expression must be substantially increased to be applicable for human gene therapy [19] The Epstein Barr virus (EBV) plasmid is a replicating episomal vector that has been developed to ... replication Baculoviruses appear to be useful viral vectors, not only for the abundant expression of foreign genes in insect cells, but also for efficient gene delivery to the hepatoma lines HepG2 and Huh7...

Ngày tải lên: 12/08/2014, 04:20

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Báo cáo y học: " In silico modeling indicates the development of HIV-1 resistance to multiple shRNA gene therapy differs to standard antiretroviral therapy" docx

... description CD34+ HSC were assumed to have been transduced with the gene therapy ex vivo and returned to the patient to engraft and to continuously give rise to a supply of gene- containing CD4+ progeny ... C46) [49-51] Conclusions In summary, resistance to gene therapy appears to differ from that for antiretroviral therapy Although HSC gene therapy aims to establish a large protected population of ... multiple shRNA gene therapies may need to be combined with a Class I inhibitor to allow Tx cells to survive and mitigate the effects of HIV Methods This stochastic model of gene therapy for HIV...

Ngày tải lên: 13/08/2014, 01:20

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Báo cáo y học: " Human T lymphotropic virus type-1 p30II alters cellular gene expression to selectively enhance signaling pathways that activate T lymphocytes" doc

... changes in gene expression, associated with apoptosis, cell cycle, DNA repair, signaling factors, immune modulators, cytokines, growth factors, and adhesion molecules Recently, Affymetrix, GeneChip ... Similar to the effect of HTLV-1 Tax on apoptosis related genes [24,27], we found that p30II also deregulates multiple genes resulting in possible pro-apoptotic and anti-apoptotic effects Since apoptosis ... Sherlock G: Gene ontology: tool for the unification of biology The Gene Ontology Consortium Nat Genet 2000, 25:25-29 Konikova E, Kusenda J: Altered expression of p53 and MDM2 proteins in hematological...

Ngày tải lên: 13/08/2014, 13:20

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Báo cáo sinh học: "The use of retroviral vectors for gene therapy-what are the risks? A review of retroviral pathogenesis and its relevance to retroviral vector-mediated gene delivery" pptx

... (86) to transduce PHSC with shorter exposure to less cytokines needs to be fully explored 6) In general the limitations of vectors should be taken into account when designing gene therapy protocols ... oncogenic retroviral vectors and the protocol used was designed to enhance transduction by using multiple cytokines to stimulate division of PHSC This is likely to induce many genes involved in regulating ... oncogene activation Given the historical difficulty of obtaining good transgene expression from MoMLV derived vectors in haematopoietic lineages, and the lack of evidence to suggesting that oncogene...

Ngày tải lên: 14/08/2014, 19:22

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Báo cáo y học: "Comparison of osteogenic potentials of human rat BMP4 and BMP6 gene therapy using [E1-] and [E1-,E2b-] adenoviral vectors"

... appear to tolerate firstgeneration adenoviral vectors, and exposure to adenoviral antigens still elicits the generation of a robust immune response [7] The overexpression of self-transgenes may, ... and E2b genes and the use of homologous (rat) BMP genes not improve the osteogenic potential of direct BMP adenovirus vector gene therapy, the results of this study indicate that BMP gene therapy ... self-transgenes may, therefore, lead to an autoimmune response and potentially significant side-effects Thus, human gene therapy trials in which an adenoviral vector containing human genes is utilized should...

Ngày tải lên: 31/10/2012, 17:08

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Thu thập nguồn gene và tổ chức dữ liệu gene 1

... THO PhD TRI BUI MINH PhD VU NGUYEN CONG The purpose of this research is to collect, orgarnize gene data to surve research in genetic modified plant The research was carried out at the Chemical and ... đƣợc chép DNA (gene) 12 Hình 1.11: Cắt DNA Plasmid sử dụng enzyme cắt giới hạn 13 Hình 1.12: Gắn gene chuyển vào vector (Plasmid) 13 Hình 1.13: Plasmid dùng chuyển gene đậu nành ... nành 15 Hình 1.14: Chuyển gene thông qua môi trƣờng Agrobacterium tumefaciens 16 Hình 1.15: Súng bắn gene đƣợc dùng chuyển gene 16 Hình 1.16: Chuyển gene thông qua vi 17...