gene therapy in the heart

... bypass intracellular processing to provide gene delivery to the nucleus In the context of gene therapy, the delivery of therapeutic genes by liposomes can also result in the inhibition of angiogenesis ... extensively investigated is the Echerichia coli cytosine deaminase (CD) gene plus 5-fluorocytosine (5-FC) The CD gene converts 5- 252 GENE THERAPY IN THE TREATMENT OF CANCER FC to the chemotherapeutic ... molecules The first is the establishment of the relevance of the genetic basis of carcinogenesis Second is the determination of the specific oncogene to target Third is the determination of the specific...

Ngày tải lên: 10/04/2014, 22:11

... Results and Discussion The main finding of this study is the identification of a metabolic gene switch from fatty acids to glucose in the murine female heart at baseline linked to enhanced mitochondrial ... additional insights into this interesting question Conclusions In summary, we have identified a novel metabolic gene switch (decrease in fatty acid utilization, increase in glucose utilization) in the ... McClain DA, Litwin SE et al (2002) Insulin signaling coordinately FEBS Journal 274 (2007) 5278–5284 ª 2007 The Authors Journal compilation ª 2007 FEBS 5283 Metabolic gene switching in murine female...

Ngày tải lên: 18/02/2014, 16:20

... thus the former is the vector of choice for future gene therapy trials The number of gene therapy trials in RA is in sharp contrast to the more than 600 clinical trials of gene therapy in patients ... local gene therapy is the reduction in local production of proinflammatory cytokines, thereby reducing their circulating levels and abolishing a sensitizing effect on naïve joints A conflicting finding ... complexes with the immunoglobulin domain of the tgAAC94 fusion protein, thereby inactivating the therapeutic protein in the arthritic joint Moreover, epidemiological studies showed that 80% of the human...

Ngày tải lên: 09/08/2014, 01:24

... Genetics; and TissueGene References Evans CH, Rediske JJ, Abramson SB, Robbins PD: Joint efforts: tackling arthritis using gene therapy First International Meeting on the Gene Therapy of Arthritis ... accomplishment because there was a concern that the high synthesis of the α2-chain would disrupt the 2:1 ratio of α1:α2 chains in type I collagen Instead, the inherent editing functions of the cell ensured ... for gene therapy Indeed, these disciplines may find themselves Available online http://arthritis-research.com/content/7/6/273 in the forefront when it comes to clinical applications Many of these...

Ngày tải lên: 09/08/2014, 07:20

... Purified recombinant GST-RELA peptides (amino terminus, DNA binding domain; carboxyl terminus, transactivation domain; in the left panel, the arrow indicates the GST-RELA 428-551 amino acid peptide) ... the cooperation between three transcription factors in a multi-protein complex may control gene expression program in heart failure Understanding the way that different DNA binding proteins interact ... p53RELA interaction was direct and dependent on the carboxy-terminal transactivation domain of RELA because a purified recombinant GST fusion protein of the RELA carboxy-terminal domain, but not the...

Ngày tải lên: 11/08/2014, 12:20

... desert, farther and farther from the lost track where the pilot lay in his blood; and still the mocking spirits of the desert, the afreets of the mirage, led them on, and the hike glistening in the ... and there, in the distinct forms in which the camels had gasped their last The dry desert air had converted the hide into a coffin There were no flies here, thus there were no worms to devour the ... ago The conversation of the Arabs is in the exact style of the Old Testament The name of God is coupled with every trifling incident in life, and they believe in the continual action of divine...

Ngày tải lên: 15/02/2014, 09:20

... 6) for the remaining introns Finally, the 3¢ UTRs of the guinea pig CYP11B genes show only 42% similarity whereas the 3¢UTR of the guinea pig CYP11B2 gene shares up to 72% identity with the corresponding ... position 366 while all other proteins of the CYP11B family including CYP11B2 of the guinea pig have a basic residue (arginine or lysine, respectively) at the corresponding position These two basic residues ... showed the guinea pig together Table Pair-wise sequence similarities of CYP11B proteins Similarities for the CYP11B proteins were determined pair-wise using the PALIGN program (PcGENE, Intelligenetics)...

Ngày tải lên: 22/02/2014, 07:20

... lipid-binding protein, intestinal 15 kDa protein, ileal bile acid-binding protein and gastrotropin, reflecting the speculations of authors on its intracellular function(s) In vitro binding assays ... from the AB strain of zebrafish (see http://zfin.org for strain details) The zebrafish fabp6 gene The coding sequence of the fabp6 gene contained an open reading frame of 393 bp (not including the ... detected in the duodenum, jejunum or 12 other extraintestinal tissues (the latter tissues were not specified) The transcriptional initiation of the zebrafish fabp6 gene in the distal region of the intestine...

Ngày tải lên: 07/03/2014, 06:20

... that of a room at the Sorbonne." There was the incident of the wandering of Nicolas Aubry, "more skilled in the devious windings of the [Latin Quarter] than in the intricacies of the Acadian Forest," ... home, gathered the tribes of the west into a confederacy against the Iroquois, as Champlain had done in the east, gave up for the present the building of the vessel, and in 1681, the river being ... wool of the buffalo then roaming the woods and prairies and plains from one side of the valley to the other; the Indians would gather about these centres for gain and protection; and in the midst...

Ngày tải lên: 31/03/2014, 13:20

... in the use of gene transfer of the b-adrenergic recepter gene into the ailing myocardium as a means of therapeutic intervention For myocardial infarction, gene therapy offers the ability to genetically ... reduction in neointima formation Bioengineering and Gene Therapy The use of gene transfer in vein grafts may go beyond the treatment of the graft itself The thrombogenicity of prosthetic materials, ... adenosine monophosphate that then leads to increased levels of hypoxanthine within and around cells GENE THERAPY FOR THE HEART 197 in the affected area Additionally, increased conversion of xanthine...

Ngày tải lên: 10/04/2014, 22:11

... with Survivin levels indicates the increase in risk of death for 100 fold increase in Survivin expression (Table 3) Since only these four independent variables were retained by the Cox model, this ... Our findings are of particular relevance also from the tumor biology viewpoint because the Survivin gene encodes a key anti-apoptotic protein belonging to the inhibitor of apoptosis protein (IAP) ... Competing interests Gene expression levels of Survivin add significant prognostic value to the current TNM staging system of patients with gastric carcinoma The validation of these findings in larger...

Ngày tải lên: 18/06/2014, 15:20

... analyzed the potential influences of the CTLA-4 genotype upon the outcome of IFN adjuvant therapy, on the basis of prior suggestions of the role of certain polymorphisms of the CTLA-4 gene and other ... further explored in other trials of IFN-a2b of the US and European cooperative groups Our investigation into the association of CLTA-4 polymorphisms and the results of interferon therapy in a ... than a year from the start of IFNa-2b therapy, the development of autoimmunity per se could not serve as a criterion for selecting patients to initiate therapy The human CTLA-4 gene is located...

Ngày tải lên: 18/06/2014, 16:20

... (in bold type) Genesto black mice involved in cholesterol biosynthesis exhibiting elevated expression (p < 0.05) in 180-day LY mice as comGenes (in bold type) involved in cholesterol biosynthesis ... of particular interest due to the opposing action of their protein products and divergence in their expression in obese LY mice These enzymes interconvert corticosterone to its inactive metabolite ... This is the first report of differences in the levels of ovarian gene expression in an obese mouse model The most important finding of this study is that modified gene expression in the ovaries...

Ngày tải lên: 20/06/2014, 07:20

... CA) The repression activity of the Mad-SID is mediated through interactions of the Mad N-terminus with the mammalian Sin3 co-repressor protein [10,11] The effectiveness of the Sin3 binding domain ... is involved in modulating the signaling cascade activated by PRL in granulosa cells [48] The Ctla4, Eda, and Mrgpre genes are in the signaling category but are not found in the OKDB However, they ... by binding to AP-1 binding site elements [12] Another group of investigators fused Mad SID to the tetracycline receptor and demonstrated tetracycline mediated gene repression via binding to the...

Ngày tải lên: 20/06/2014, 07:20

... Main Viral Vectors Features Retroviral Lentiviral Adenoviral AAV Hum Foamy Vir advantages gene transfer in gene transfer in effective dividing cells in few gene expre transduced transducing inflammatory ... in vivo, in which the vector is directly injected into the patient or, in the case of hematopoietic and some other target cells, ex vivo, with removal of the target cells from the patient, followed ... which the DNA or RNA is delivered The series of steps in which the donated DNA enters the target cell and begins expression is referred to as transduction Gene delivery can take place in vivo, in...

Ngày tải lên: 07/07/2014, 01:20

... efficiency The first convincing therapeutic effect from gene transfer occurred with Xlinked severe combined immunodeficiency disease (SCID), which results from mutations in the gene (IL2RG) encoding the ... the evolution of gene therapy They demonstrated conclusively that gene therapy could cure disease; of the 16 infants eventually treated in these trials, 15 achieved correction of the immunodeficiency ... complex involved in hematopoietic development Insertion of the retroviral long terminal repeat is thought to increase the expression of LMO-2 The X-linked SCID studies were a watershed event in the...

Ngày tải lên: 07/07/2014, 01:20

... likely be tested in upcoming trials Administration of an AAV vector expressing factor IX to the liver in humans with hemophilia resulted in therapeutic circulating levels at the highest dose ... but these require repeated intravitreal injection of the siRNAs; an AAV vector–mediated approach might allow long-term knockdown of VEGF Gene Therapy for Cancer The majority of clinical gene ... the genetic disease, although the exact limitations imposed by age await clinical studies AAV-RPE65 trials have now been approved in both the United States and Great Britain Other inherited retinal...

Ngày tải lên: 07/07/2014, 01:20

... domain fused to an intracellular signaling domain to allow T cell activation, thereby converting normal lymphocytes into cells capable of recognizing and destroying tumor cells A third immunotherapy ... transplantation, is being investigated as a strategy for allowing administration of higher doses of chemotherapy than would otherwise be tolerated Gene Therapy for Vascular Disease The third major ... hematopoietic cells with genes encoding resistance to chemotherapeutic agents, including the multidrug resistance gene MDRI or the gene encoding O6-methylguanine DNA methyltransferase (MGMT) Ex...

Ngày tải lên: 07/07/2014, 01:20

... recombinant proteins are recent examples Gene therapeutics, which entered clinical testing in the early 1990s, are well along in the course of development, and are likely to become increasingly ... enrolled in gene therapy trials (Table 65-3) Realization of the therapeutic benefits of the Human Genome Project, and of new discoveries such as RNAi, will depend on continued progress in gene transfer ... non-integrating (plasmid, adenoviral, AAV)? What was the route of administration of the vector? What was the target tissue? What gene was transferred in? A disease-related gene? A marker? Were there...

Ngày tải lên: 07/07/2014, 01:20

... 2-methoxyestradiol, decreasing 3, 4-estradiol quinone or indirectly via the inactivation of E2 The importance of the CYP1A1/CYP1B1 ratio in- vivo is evident in the increased presence of 4-OH-E2 in breast cancer ... which iodine/iodide could enhance the efficacy of Tamoxifen therapy: 1) having an additive effect on estrogen inhibition and 2) inhibiting the expression of CCND1 thus preventing or slowing the development ... current therapies In this study we provide the first gene array profiling of an estrogen responsive breast cancer cell line demonstrating that the combination of iodine and iodide alters gene expression...

Ngày tải lên: 08/08/2014, 17:20