... (green) and nuclear DAPI (blue) fluorescence The images were recorded with an exposure time of 50 ms for DAPI and 500 ms for EGFP (1 s for negative expression) Page of (page number not for citation ... number not for citation purposes) Genetic Vaccines and Therapy 2009, 7:9 Acknowledgements http://www.gvt-journal.com/content/7/1/9 18 We thank Günter Schwarz (Köln) for providing cPMP and Sebastian ... AAV-MOCS1 for systemic delivery, as compared to × 1010 tu for the intrahepatic injections described previously [5] and above, the results described here indicate a similar efficacy for both application...
Ngày tải lên: 14/08/2014, 19:22
... E1 and E2b genes and the use of homologous (rat) BMP genes not improve the osteogenic potential of direct BMP adenovirus vector gene therapy, the results of this study indicate that BMP gene therapy ... patterns of bone formation for [E1-,E2b-]ADhBMP6 and [E1-,E2b]ADGFP&hBMP6 were observed in both AN and SD rats Int J Med Sci 2006, 103 Figure BMP4- and BMP6-induced bone formation in AN and SD rats ... (Lanes 1, and 3), BstXI (Lanes 4, 5, and 6), and BglII plus EcoRV (Lanes 7, 8, and 9) M, 1-kb DNA ladder Lanes 1, 4, and 7, 293A cells; Lanes 2, 5, and 8, ADrBMP6 DNA; Lanes 3, 6, and 9, ADhBMP6...
Ngày tải lên: 31/10/2012, 17:08
Air Pollution Mix and Emergency Room Visits for Respiratory and Cardiac Diseases in Taipei ppt
... 10-18 for cardiac diseases, and 6-11 for gastrointestinal diseases during Air Pollution on Emergency Room Visits 315 the study period The young had the most ER visits for respiratory diseases and ... 0.38 0.61 0.72 We performed 72 single-pollutant models for respiratory diseases in three age strata, 48 models for cardiac diseases, and another 48 models for gastrointestinal diseases in two age ... to daily ER visits for both respiratory and cardiac diseases PM10 showed weaker effects on daily ER visits for respiratory and cardiac diseases in the single-pollutant model and was not as consistent...
Ngày tải lên: 29/03/2014, 18:20
gene therapy for hematological disorders
... HEMATOPOIETIC STEM AND PROGENITOR CELLS AS TARGETS FOR GENE THERAPY 143 144 GENE THERAPY FOR HEMATOLOGICAL DISORDERS restoration of cellular and humoral immunity were documented after gene therapy Data ... In vivo gene transfer is most appropriate for target cells that cannot REQUIREMENTS FOR GENE TRANSFER INTO HEMATOPOIETIC CELLS 135 TABLE 6.1 Relevant Targets and Applications for Gene Therapy ... carrying the FAC or FAA gene A possible in vivo survival advantage for gene- corrected primitive cells and their progeny has made FA an attractive candidate disease for stem cell gene therapy A clinical...
Ngày tải lên: 10/04/2014, 22:10
gene therapy for liver disease
... attempted so far GENERAL PRINCIPLES FOR HEPATIC GENE THERAPY There are two basic approaches for gene transfer into hepatocytes: ex vivo and in vivo strategies (Fig 7.1) Ex vivo therapy requires ... therapeutic gene. Alternatively, different viral vectors as well as liposomes can be used for ex vivo gene transfer GENERAL PRINCIPLES FOR HEPATIC GENE THERAPY 155 Collagenase treatment and hepatocyte ... preferrable for vectors without liver targeting abilities For in vivo gene therapy, the therapeutic or normal gene is introduced directly into the host On one hand, in vivo gene therapy circumvents...
Ngày tải lên: 10/04/2014, 22:11
gene therapy for hiv infection
... gene expression Thus, the antisense gene expression must be quantatively higher than the levels of HIV-1 gene expression for an antisense gene therapy strategy to be effective Standard gene therapy ... study for use in HIV gene therapy Gene Therapy 6:913–921, 1999 Smith C, Sullenger BA AIDS and HIV infection In Dickson G (Ed.), Molecular and Cell Biology of Human Gene Therapeutics Chapman and ... PRACTICAL ASPECTS OF GENE THERAPY FOR HIV Cellular Targets for Gene Therapy The dominant reservoir of HIV-1 infection and replication are the cells of lymphoid (lymphopcytes) and myeloid (macrophages,...
Ngày tải lên: 10/04/2014, 22:11
cognitive therapy for chronic and persistent depression - r. moore, a. garland (wiley, 2003)
... COGNITIVE THERAPY FOR CHRONIC AND PERSISTENT DEPRESSION Richard G Moore Addenbrooke’s Hospital, Cambridge and Anne Garland Nottingham Psychotherapy Unit COGNITIVE THERAPY FOR CHRONIC AND PERSISTENT ... Fraser Watts and Mark Williams, whose helpful influence pervades every page r My family and friends for their unwavering support, and especially Stella and the boys for their love and forbearance ... units and specialist psychotherapy services Anne is also recognised nationally as a cognitive therapy trainer and clinical supervisor and has presented cognitive therapy skills based workshops and...
Ngày tải lên: 12/05/2014, 17:19
Fungicides for Plant and Animal Diseases Part 2 doc
... favan-3-ol and are found in apples, grapes, strawberries, plums, sorghum and barley (Davidson, 1997) 14 Fungicides for Plant and Animal Diseases Benzoic acid, proanthocyanidins and flavonols account for ... antifungal agents 30 Fungicides for Plant and Animal Diseases that degrade cell walls and inhibit the synthesis of mannan and β-glucan enzymes, antiparasitic agents and insecticidal agents Actinobacteria ... be toxic to pathogens and which are formed by peroxidase activity during the deposition of cell wall compounds (Goodman & Novacky, 1994) 22 Fungicides for Plant and Animal Diseases Several glucanohydrolases...
Ngày tải lên: 22/06/2014, 03:20
Fungicides for Plant and Animal Diseases Part 4 ppt
... 52 Fungicides for Plant and Animal Diseases Oki, T., Tenmyo, O., Hirano, M., Tomatsu, K and Kamei, Hl, 1990b Pradimicins A, B and C: New antifungal antibiotics II In vitro and in vivo biological ... al., 1995b and 2003a) The hydrophobicity of 56 Fungicides for Plant and Animal Diseases molecules is often associated with biological action (Hansch and Dunn, 1972) However, the rationale for this ... 1995; Kubo and Kubo, 1995; Kubo et al., 1996 and 1999; Kubo and Fujita, 2001; Trombetta et al., 2002) and readily available Therefore, a homologous series of aliphatic 2E-alkenals and the corresponding...
Ngày tải lên: 22/06/2014, 03:20
Fungicides for Plant and Animal Diseases Part 6 doc
... Aspergillus, Cladosporium and Fusarium Aspergillus strains Rhizoctonia solani, Collectotrichum gloeosporioides, Fusarium solani and Ganoderma australe Candida albicans and Candida sp Trichophyton ... Fungicides for Plant and Animal Diseases Plant Specie Plant pathogen Fungicidal activity concentrations References Microsporum canis, Allescheria boydii and Pleurotus ostreatus, Drechslera rostrata Candida ... medicinal plants and herbal products for fungal infection related diseases control program because plant derived drugs are considered safe and free from adverse side effects Plants generally produce...
Ngày tải lên: 22/06/2014, 03:20
Fungicides for Plant and Animal Diseases Part 7 pot
... Genomic and Biotechnology, University of Trás-os-Montes and Alto-Douro, Department of Genetics and Biotechnology 2CECAV- Center for Animal Science and Veterinary, University of Trás-os-Montes and ... P>0.05) Cunha and Fernandes-Ferreira (1999) and Harada e Murai (1996) obtain similar results with Linum usitatissium L and Prunus mume, respectively 122 Fungicides for Plant and Animal Diseases A ... Multiplication of Aromatic and Medicinal Plants and Fungicide Activity Fernanda Leal1, Manuela Matos1, Ana Cláudia Coelho2 and Olinda Pinto-Carnide1 1IBB-Institute for Biotechnology and Bioengineering,...
Ngày tải lên: 22/06/2014, 03:20
Fungicides for Plant and Animal Diseases Part 8 doc
... Institute for Molecular and Cell Biology for providing seeds for in vitro establishment of Catharanthus roseus The authors also like to thank to Carina Alves, Luís Silva, Sandra Cabo and Tatiana ... Production and Essential Oil Quality 138 Fungicides for Plant and Animal Diseases Journal of Agricultural and Food Chemistry, Vol 52, Nº.17, pp 5418-5424, ISSN 00218561 Souza, E.; Stamford, T.; ... single value observed per bacterium, and Xavg and s are the average and standard deviation, respectively, of all values observed for the isolate group Using the formula RPI = (F + 2) x 100/4, data...
Ngày tải lên: 22/06/2014, 03:20
Fungicides for Plant and Animal Diseases Part 10 doc
... Conference-Pest and Diseases, 2, 375-382 180 Fungicides for Plant and Animal Diseases Reddy, S., Spencer, J.A & Newman S.E (1992) Leaflet surfaces of blackspot-resistant and susceptible roses and their ... including many genes that are induced during infection 186 Fungicides for Plant and Animal Diseases stage These fast-evolving effector genes are localized to highly dynamic and expanded regions ... any such gene could became a target for disease control The types of genes essential for pathogenesis depend on the infection process of a particular fungus Some fungi degrade the cuticle and cell...
Ngày tải lên: 22/06/2014, 03:20
Fungicides for Plant and Animal Diseases Part 12 potx
... Fungicides for Plant and Animal Diseases Chemical control of BLSD would not appear sustainable in the long term In several countries, fungicide resistance to systemic fungicides is increasing, and chemical ... widespread in the Pacific Islands and also mention that they are virulent on Yangambi km which is also considered as highly resistant to BLSD 220 Fungicides for Plant and Animal Diseases strategy must ... implementation and research teams able to provide support for anticipation and adaptation Acronyms AA: diploid Acuminata AAA: triploid Acuminata BGA: Banana Growers Association 222 Fungicides for Plant and...
Ngày tải lên: 22/06/2014, 03:20
Fungicides for Plant and Animal Diseases Part 15 ppt
... Fungicides for Plant and Animal Diseases was formerly used for analysis of carbon monoxide content in gases, for example in Hempel's gas apparatus The moist powder’s exposure to air and sunlight, ... the formula CuCl2 x 3Cu(OH)2, a green powder used as a blue colour agent and as a fungicide (form as powder, wettable powders and pastes) that controls a wide range of fungal and bacterial diseases ... copper nitrate, its chemical formula is Cu(NO3)2 (Mr = 232.60 g mol-1) In anhydrous form it is blue coloured, in crystalline and it is used for formulation of fungicides and herbicides The production...
Ngày tải lên: 22/06/2014, 03:20
Báo cáo y học: "The 3rd International Meeting on Gene Therapy in Rheumatology and Orthopaedics" pot
... Targeted Genetics; and TissueGene References Evans CH, Rediske JJ, Abramson SB, Robbins PD: Joint efforts: tackling arthritis using gene therapy First International Meeting on the Gene Therapy ... differentiation, and application in cell and gene therapy J Cell Mol Med 2004, 8:301-316 23 Gafni Y, Turgeman G, Liebergal M, Pelled G, Gazit Z, Gazit D: Stem cells as vehicles for orthopedic gene therapy Gene ... been generated in various animal models of arthritis, tissue repair, disc degeneration, and OI It is encouraging that several clinical trials have been initiated for the gene therapy of RA, and...
Ngày tải lên: 09/08/2014, 07:20
Báo cáo khoa học: " Stereotactic body radiation therapy for melanoma and renal cell carcinoma: impact of single fraction equivalent dose on local control" ppt
... term survivors and chance for cure with a year OS rate of 39% [30] The argument for using ablative local therapy for isolated metastases is strengthened if effective systemic therapy is available ... single high dose SRS to brain and spinal metastases for both melanoma and RCC [8-10,26] In these studies the LC for melanoma is typically lower than for RCC [10,26], for which brain SRS can achieve ... regimen for Figure Change in SUV for controlled lesions The Standardized uptake values were plotted with pre-treatment PET used for planning as time Follow-up PET/CT’s were fused and SUV was generated...
Ngày tải lên: 09/08/2014, 09:20
Báo cáo y học: " Taking stock of gene therapy for cystic fibrosis" pdf
... and containing numerous submucosal glands, and the small bronchiolar regions lined by a simple columnar epithelium devoid of glands A central question for CF gene therapy is which cell type and ... answered for successful CF gene therapy have now been clearly defined, and gene therapy for CF remains the most promising possibility for curative rather than symptomatic therapy 12 13 14 15 16 References ... has dogged the recent media view of gene therapy Gene therapy is clearly at the typical stage of new drug development where considerable and unglamorous effort needs to be expended to move from...
Ngày tải lên: 12/08/2014, 18:20
Báo cáo sinh học: "Careful adjustment of Epo non-viral gene therapy for β-thalassemic anaemia treatment" pot
... Therefore, in the particular case of Epo, an accurate level of transgene expression is required for safety reasons Temporal control systems of transgene expression have already been used in gene therapy ... duration and delivered at a frequency of Hz, using plate electrodes and generator BTX ECM 830 (Genetronics™), as previously described [20] Page of (page number not for citation purposes) Genetic ... 91 and then from day 189 to day 271 for the 1.5 μg group (p values of 0.002 on day 91, 0.005 on day 189, 0.002 on day 210, 0.01 on day 241 and 0.002 on day 271) and at day 91, 189 and 241 for...
Ngày tải lên: 14/08/2014, 19:22
nonviral vectors for gene therapy, methods and protocols
... Nazarova, O., and Seymour, L W (1996) Characterization of vectors for gene therapy formed by self-assembly of DNA with synthetic block co-polymers Hum Gene Ther 7, 2123–2133 11 Kabanov, A V and Kabanov, ... 3.3.), and by NMR For 1H-NMR assignments, see Subheading 3.1.8 and Notes and 3.3 GPC of pDMAEMA Homo- and Copolymers This instruction is intended for the molecular weight determination of pDMAEMA and ... of vectors for gene therapy formed by self-assembly of DNA with synthetic block co-polymers Hum Gene Ther 7, 2123–2133 Kataoka, K., Togawa, H., Harada, A., Yasugi, K., Matsumoto, T., and Katayose,...
Ngày tải lên: 11/04/2014, 09:59