gene therapy for hematologic disorders human immunodeficiency virus infection and cancer

... in patients with HIV infection [2-8] Three had co-infections with hepatitis C virus and were receiving interferon therapy [6-8] and six more had AIH before starting HAART and one pediatric patient’s ... anti-cardiolipin and anti-ribosomal antibodies; anti-double-stranded DNA (anti-dsDNA) titer, 1:160; and immunoglobulin G level, 4600 mg/dL Her antibodies to hepatitis viruses A, B and C and hepatitis ... for the patient and edited the manuscript HN reviewed and provided legends for the pathological images MH reviewed the pathological specimens and made the initial diagnosis All authors read and...

Ngày tải lên: 10/08/2014, 23:21

... HEMATOPOIETIC STEM AND PROGENITOR CELLS AS TARGETS FOR GENE THERAPY 143 144 GENE THERAPY FOR HEMATOLOGICAL DISORDERS restoration of cellular and humoral immunity were documented after gene therapy Data ... the endogenous gene product 150 GENE THERAPY FOR HEMATOLOGICAL DISORDERS In a murine allogeneic skin transplantation model, foreign genes expressed by hematopoietic stem cells and their progeny ... ability Blood 92:1131–1141, 1998 152 GENE THERAPY FOR HEMATOLOGICAL DISORDERS Gene Therapy Blaese RM, Culver KM, Miller AD, et al T lymphocyte-directed gene therapy for ADASCID: Initial trial results...

Ngày tải lên: 10/04/2014, 22:10

... development, expanding HIV testing and treatment, discovering and developing new ARV drugs, optimizing the ART delivery system, and improving therapy for non-AIDS related conditions and coinfections ... tolerance by expanding methadone maintenance treatment (MMT) and needle and syringe programs (NSPs), removing the requirement for viral hepatitis testing before school and job entry, and lifting ... AIDS Research and Therapy 2010, 7:12 http://www.aidsrestherapy.com/content/7/1/12 cations and cancer HPTN052 is a multinational trial of 1750 subjects with HIV infection who have been randomized...

Ngày tải lên: 10/08/2014, 05:21

... cell types and viruses, once a candidate gene is identified, as we reported for HIV-1 infection [1] In this study, we identified ADAM10 by gene trap insertional mutagenesis as a disrupted gene in ... required for replication of human immunodeficiency virus type 1, filoviruses, and measles virus J Virol 2005, 79:11742-11751 Organ EL, Sheng J, Ruley HE, Rubin DH: Discovery of mammalian genes that ... Brackmann K, Grandgenett D: Concerted integration of retrovirus-like DNA by human immunodeficiency virus type integrase J Virol 1995, 69:6090-6097 10 Greene WC: The molecular biology of human immunodeficiency...

Ngày tải lên: 13/08/2014, 01:20

... with HIV between 1988 and 1992 through the use of blood and blood products unscreened for HIV antibodies, and the use and reuse of disposable needles and syringes in hospitals and institutions.[1] ... transmission, Romanian children and adolescents with horizontally acquired HIV infection also may be at substantial risk for infection with hepatitis B virus (HBV) and hepatitis C virus (HCV) Although nosocomial ... Risk factors for HIV infection among abandoned Romanian children AIDS 1993, 7:1617-1624 Abstract Bodsworth NJ, Cooper DA, Donovan B: The influence of human immunodeficiency virus type infection...

Ngày tải lên: 20/06/2014, 08:20

... MDDC and analyzed for HIV DNA copies by real time PCR Bars represent mean + standard error Page of (page number not for citation purposes) AIDS Research and Therapy 2007, 4:25 http://www.aidsrestherapy.com/content/4/1/25 ... bacterial vaginosis and HIV genital tract virus load AIDS Res Hum Retroviruses 2005, 21:945-948 Al-Harthi L, Spear GT, Hashemi FB, Landay A, Sha BE, Roebuck KA: A human immunodeficiency virus (HIV)-inducing ... predictor of Neisseria gonorrhoeae and Chlamydia trachomatis infection Clin Infect Dis 2003, 36:663-668 Sobel JD: Gynecologic infections in human immunodeficiency virus- infected women Clin Infect...

Ngày tải lên: 10/08/2014, 05:20

... immunoglobulin-producing cells and in the process of B-cell memory, and they are not permissive for HIV infection [13] Germinal centers, however, continue to function even when infiltrated by the virus and appear ... HIV-positive for 13 years No other information about our patient was available at that time, because he was in a life-threatening condition and no time was available to ask for information from ... opportunistic infection and sepsis, and its role should be useful in patients with HIV Other studies are needed to validate this hypothesis Consent Written informed consent was obtained from the patient for...

Ngày tải lên: 10/08/2014, 22:24

... of Turku, Turku, Finland Authors’ contributions TM and SMT performed experiments RV collected and characterized the human sera samples SS and NK designed the HIV antigen and generated the monoclonal ... assay for detecting HIV and HBV infections in human serum samples First, we used an in-house panel of 100 ‘normal’ serum samples that were confirmed to be negative for both HIV and HBV infections ... seropositive for both HIV-1 and HBV (Table 1) Out of these 13 HIV + /HBV + serum samples, were positive for HCV, and two for HTLV as well The remaining three HIV + serum samples were negative for HBV...

Ngày tải lên: 11/08/2014, 00:22

... HIV infection and TB and opportunistic infection and Sub-Saharan Africa (5 articles, review) • Tuberculosis and HAART and HIV infection and Africa (3 articles, review) • Tuberculosis and HIV infection ... infection and mortality and Sub-Saharan Africa (4 articles) • Antiretroviral adherence and East Africa (No articles) • Antiretroviral adherence and Tanzania (2 articles) • HIV infection and TB and ... Antiretroviral Therapy HIV Human Immunodeficiency Virus MDH Muhas Dar es Salaam City Harvard Collaborative Project MoHSW Ministry of Health and Social Welfare MUHAS Muhimbili University of Health and Allied...

Ngày tải lên: 22/03/2014, 18:20

... Reference gene selection for quantitative real-time PCR analysis in virus infected cells: SARS corona virus, Yellow fever virus, Human Herpesvirus-6, Camelpox virus and Cytomegalovirus infections ... SARS corona virus, yellow fever virus, human herpes virus6 , camelpox virus, CMV [7] and Epstein-Barr virus (EBV) [12] However such a study has never been carried out using the key human pathogens ... seconds 55°C for 30 seconds and 72°C for 30 seconds Data analysis The ΔΔCT [23] method was used for initial data analysis Four biological replicates were used for each infection and a two tailed...

Ngày tải lên: 18/06/2014, 18:20

... Reference gene selection for quantitative real-time PCR analysis in virus infected cells: SARS corona virus, Yellow fever virus, Human Herpesvirus-6, Camelpox virus and Cytomegalovirus infections ... SARS corona virus, yellow fever virus, human herpes virus6 , camelpox virus, CMV [7] and Epstein-Barr virus (EBV) [12] However such a study has never been carried out using the key human pathogens ... seconds 55°C for 30 seconds and 72°C for 30 seconds Data analysis The ΔΔCT [23] method was used for initial data analysis Four biological replicates were used for each infection and a two tailed...

Ngày tải lên: 20/06/2014, 01:20

... factors and its role in early events associated with virus infection Therefore, understanding the role of oligomerization in Vpr functions and disease progression may provide useful information for ... domains I, II and III dysregulate Vpr oligomerization and alter the ability of Vpr to incorporate into virus particles Page of 11 Materials and Methods Cells and plasmid HeLa, and 293T cells ... transfection, cells were washed with PBS and fixed in 3.7% formaldehyde at room temperature for 10 minutes, and washed and permeabilized with 0.5% Triton X-100 for an additional 10 minutes After washing...

Ngày tải lên: 12/08/2014, 04:20

... RANTES for two hours at 37°C (C), and then inoculated with two HIV-1 strains, GUN-7WT (m and l) and HCM342 (h and n), HIV-2 CBL23 strain (n and s), and SIV mndGB-1 strain ( and r) Six days after infection, ... Specific cell surface requirements for the infection of CD4-positive cells by human immunodeficiency virus types and and by simian immunodeficiency virus Virology 1991, 181:703-715 Dalgleish ... JP: Use of inhibitors to evaluate coreceptor usage by simian and simian/ human immunodeficiency viruses and human immunodeficiency virus type in primary cells J Virol 2000, 74:6893-6910 Farzan...

Ngày tải lên: 13/08/2014, 05:21

... proto-oncogene may induce transformation of the cell Gene therapy approaches suffer most from these effects and recently it has been demonstrated that the activation of an oncogene caused transformation ... the provirus within one of the alleles These results are discussed in light both of HIV-1 pathogenesis and of the potential use of lentiviral vectors for gene therapy applications Results Generation ... of human immunodeficiency virus type Tat protein and Sp1 transcription factor J Virol 1993, 67:6224-6233 Macian F, Rao A: Reciprocal modulatory interaction between human immunodeficiency virus...

Ngày tải lên: 13/08/2014, 05:21

... K: Augmentation of virus secretion by the human immunodeficiency virus type Vpu protein is cell type independent and occurs in cultured human Page 14 of 15 (page number not for citation purposes) ... J, Kirchhoff F: Contribution of Vpu, Env, and Nef to CD4 down-modulation and resistance of human immunodeficiency virus type 1-infected T cells to superinfection J Virol 2006, 80:8047-8059 Schwartz ... endoplasmic reticulum by the human immunodeficiency virus type glycoprotein precursor J Virol 1990, 64:5585-5593 Willey RL, Maldarelli F, Martin MA, Strebel K: Human immunodeficiency virus type Vpu protein...

Ngày tải lên: 13/08/2014, 05:22

... Olson AJ, Elder JH: Structural basis for distinctions between substrate and inhibitor specificities for feline immunodeficiency virus and human immunodeficiency virus proteases J Virol 2003, 77:6589-600 ... Italy, for mathematical advice; and George E Parris (Ph.D.), Gaithersburg, MD, for the linguistic revision and for critically reading the manuscript Special thanks are given to Merck and Co for ... [REFSEQ: NC_001654.1]; human immunodeficiency virus type-1 (HIV1) [PDB: 1BL3C]; simian immunodeficiency virus, host: macaque (SIV-mac) [PDB: 1C6VC]; feline immunodeficiency virus, host: domestic...

Ngày tải lên: 13/08/2014, 05:22

... providing NLlucBglD64E provirus and Dr Kevin Coombs for critical reading of the manuscript We are also grateful to Drs M Emerman and D Grandgenett for the HeLa-CD4-CCR5-β-Gal cells and anti-IN antiserum ... domain of human immunodeficiency virus type integrase: critical residues for protein oligomerization and DNA binding J Virol 1998, 72:4841-4848 Wiskerchen M, Muesing MA: Human immunodeficiency virus ... conserved region of human Alu sequence and in HIV-1 LTR The primer used for both of the second nested PCR and for generating a probe are 5'-NI: 5'-CACACACAAGGCTACTTCCCT-3' and 3'-NI: 5'-GCCACTCCCCAGTCCCGCCC3'...

Ngày tải lên: 13/08/2014, 09:21

Ngày tải lên: 15/02/2014, 13:20

... treatment Gene therapy appears to be a good candidate for future HIV treatment options Vaccine and Treatment    It is difficult to make a vaccine for HIV due to several characteristics of the virus ... World Pandemic     HIV has enormous social, economic and humanitarian implications HIV and AIDS is decimating populations, taxing health infrastructures, and crippling economies, and creating ... mother) and after birth (for the baby)   Does not spread through mucous membranes, only blood contact  HIV is a fragile virus Tip of the Day: Use condoms and clean needles! HIV-1 and HIV-2...

Ngày tải lên: 15/03/2014, 12:57

... Assembly Naked virus: capsid + viral genome → nucleocapsid (virion) Enveloped virus: nucleocapsid + envelope → virion Site: a DNA viruses (except poxvirus): cell nucleus; b RNA viruses and poxvirus: ... Defective viruses – Abortive infection II Replication Defective viruses: are genetically deficient and incapable of producing infectious progeny virions Helper virus: can supplement the genetic ... deficiency and make defective viruses replicate progeny virions when they simultaneously infect host cell with defective e.g., AAV & adenovirus viruses Defective Viruses • Defective Viruses lack gene( s)...

Ngày tải lên: 15/03/2014, 13:00