gene silencing using rna interference in embryonic stem cells pdf
Regulation of gene expression by esrrb in embryonic stem cells
Ngày tải lên :
12/09/2015, 08:19
... small interfering RNAs (siRNAs) or plasmids expressing short hairpin RNAs (shRNAs) into cells The siRNAs introduced or produced from shRNAs by Dicer are incorporated into the RNA- induced silencing ... extra-uterine sites resulted in teratocarcinoma formation (Solter et al., 1970; Stevens, 1970) This finding indicates the embryonic origin of teratocarcinoma stem cells In 1974, the stem cells in teratocarcinomas ... factors in ES cell maintenance In ES cells, signaling pathways play important roles in maintaining self-renewal and pluripotency In most cases, these signaling pathways function through regulating gene...
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The role of kruppel like factors in embryonic stem cells
Ngày tải lên :
14/09/2015, 14:07
... protein-protein 38 Introduction interactions in protein complexes For instance, a protein interaction network in ES cells is mapped by using combination tagging and proteomics Target proteins ... trophoblast stem cells trithorax-group inactive X specific transcripts zinc finger protein 42 zinc finger protein 143 zinc finger protein of the cerebellum XI Introduction CHAPTER I INTRODUCTION Introduction ... expression RNAi-mediated pathways are induced by transfection of synthesized small interfering RNAs (siRNAs) or introduction of vectors expressing short hairpin RNAs (shRNAs) into cells shRNA will...
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Báo cáo khoa học: In vivo RNA interference in oyster – vasa silencing inhibits germ cell development pptx
Ngày tải lên :
07/03/2014, 00:20
... were injected in the gonad with 100 lL of saline solution containing dsRNA, or saline solution for the control After dsRNA injection, oysters were maintained in raceways in conditions allowing ... DIG-labelled using a T3 or T7 RNA polymerase (20 UÆlg)1 plasmid) and DIG RNA- labelling FEBS Journal 276 (2009) 2566–2573 ª 2009 The Authors Journal compilation ª 2009 FEBS 2571 In vivo RNA interference in ... induce RNAi silencing Nat Cell Biol 8, 793–802 23 Jiang Y, Loker ES & Zhang S-M (2006) In vivo and in vitro knockdown of FREP2 gene expression in the snail Biomphalaria glabrata using RNA interference...
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báo cáo hóa học:" MicroRNA and gene expression patterns in the differentiation of human embryonic stem cells" doc
Ngày tải lên :
18/06/2014, 15:20
... [16,17] and mouse embryonic stem cells [18-20] The unique patterns of miRNA expression in embryonic stem cells suggest they are involved in maintaining "stemness" Identifying mRNAs that are directly ... groups: hES cells, EB, and adult cells Node A contained the genes that were upregulated in both hES cells and EB, node B contained the genes upregulated in hES cells only, node C contained the genes ... MicroRNA expression profiling of single whole embryonic stem cells Nucleic Acids Res 2006, 34(2):e9 Chen C, Ridzon D, Lee CT, Blake J, Sun Y, Strauss WM: Defining embryonic stem cell identity using...
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Báo cáo y học: "Combinatorial RNA interference in Caenorhabditis elegans reveals that redundancy between gene duplicates can be maintained for more than 80 million years of evolution" pdf
Ngày tải lên :
14/08/2014, 17:22
... targeting multiple genes in C elegans using RNAi by bacterial feeding ('combinatorial RNAi') on a large scale We recently developed HTP methods for using RNAi by feeding to target single genes ... (c) Figure animal Combinatorial RNA interference (RNAi) can target two genes in the same Combinatorial RNA interference (RNAi) can target two genes in the same animal Exposing worms to a mixture ... 7:R69 information C elegans duplicate gene pairs (Interaction Gene1 + Gene2 ) displaying synthetic phenotypic effects upon combinatorial RNA interference (RNAi) in the RNAi-hypersensitive strain...
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báo cáo hóa học:" Transplantation of vascular cells derived from human embryonic stem cells contributes to vascular regeneration after stroke in mice" docx
Ngày tải lên :
18/06/2014, 15:20
... purposes) Journal of Translational Medicine 2008, 6:54 cells, the number (cells/ mm2/mouse) of single stranded DNA (ss-DNA)+ cells in one field in the ischemic core from each mouse in the saline- or ... neuroprotection in the ischemic brain human embryonic stem cells diferentiationon OP9 feeder Day 10 Day VEGF-R2(+) / VE-cadherin(+) / TRA-1(-) cells VEGF-R2(+) / VE-cadherin (-) / TRA-1 (-) cells expansion ... VEGF-R2-positive cells induced from undifferentiated mouse embryonic stem (ES) cells can differentiate into both VE-cadherin-positive endothelial cells (ECs) and αSMA-positive mural cells (MCs),...
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Báo cáo hóa học: "Inhibiting adenoid cystic carcinoma cells growth and metastasis by blocking the expression of ADAM 10 using RNA interference" pdf
Ngày tải lên :
18/06/2014, 16:20
... cystic carcinoma RNA interference (RNAi) was applied to inhibit the expression of ADAM 10 in an adenoid cystic carcinoma cell line with high metastatic potential, and the changes in biological ... biotinylated antibody for 30 at room temperature and visualized using diaminobenzidine as a chromogenic substrate The sections were then counterstained with hematoxylin Immunostaining was defined ... supporting the role of ADAM 10 in cell growth in SACC-LM cells (Figure 5C) In addition, the affect of gene silencing of ADAM 10 on the cell migration ability of SACC-LM cells was also investigated...
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Báo cáo sinh học: "Functional genomics of cell morphology using RNA interference: pick your style, broad or deep" doc
Ngày tải lên :
06/08/2014, 18:20
... Cofilin (twinstar) + + + + + Actin interacting protein 1; Aip1 NT + NT + NT Cell type: Study: Cytokinesis Protein (gene) * Formin (diaphanous) NT + + - + Anillin (scraps) + + + - + Kinesin (pavarotti) ... the following 15 RNAs: Aip1, formin, anillin, Rho1 GTPase, Aurora kinase, cortactin, Nck, Rac2, Abelsoninteracting protein, Enabled kinase, Rho kinase, PAK, Rho GEF (vav), phosphatidylinositol ... overlap in the genes studied, these studies generally agree on which depletions produce defects in cytokinesis or in the actin cytoskeleton in the lamellae of spread cells (Table 1) When initiating...
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Báo cáo sinh học: "A functional genomic analysis of cell morphology using RNA interference" pptx
Ngày tải lên :
06/08/2014, 18:20
... Cyclin-dependent kinase Protein serine/threonine kinase, cyclin-dependent protein kinase Kinase FBgn0015618 Cyclin-dependent kinase Protein serine/threonine kinase, cyclin-dependent protein kinase ... cyclin-dependent protein kinase Kc, S2R Kinase FBgn0019949 Cyclin-dependent kinase Protein serine/threonine kinase, cyclin-dependent protein kinase Kinase FBgn0016696 Pitslre Protein serine/threonine ... I: Dp and string RNAi resulting in big cells (e,f) Profile II: fizzy and polo RNAi resulting in increased frequency of cells with mitotic spindles (g) Cdk5 RNAi resulting in smaller cells and disorganized...
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Báo cáo sinh học: "RNA interference in nematodes and the chance that favored Sydney Brenner" pot
Ngày tải lên :
06/08/2014, 18:21
... dsRNAs Soaking in dsRNAs Figure Different modes of administration of dsRNAs for RNA interference External application of RNAs by soaking or feeding bacteria requires the dsRNAs to cross the intestinal ... systemic spreading of the signal Internal application by injection into the syncytial female gonad ensures transmission to the next generation, regardless of systemic spreading Intestine is in ... of dsRNAs Interestingly, C briggsae seems to be deficient in the uptake of dsRNAs in the intestine, a deficiency that can be complemented by expression of C elegans sid-2 (systemic RNA interference...
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Báo cáo y học: "Towards in vivo application of RNA interference – new toys, old problems" pdf
Ngày tải lên :
09/08/2014, 01:23
... RNA interference pathway Long double-stranded RNA (dsRNA) or small hairpin RNA (shRNA) is processed by Dicer to form a small interfering RNA (siRNA), which associates with RNA- induced silencing ... RNA interference and in synthesis of small RNA involved in developmental timing in C elegans Genes Dev 2001, 15:2654-2659 Knight SW, Bass BL: A role for the RNase III enzyme DCR-1 in RNA interference ... small interfering RNA in human cells Nat Biotechnol 2002, 20: 505-508 33 Paddison PJ, Caudy AA, Bernstein E, Hannon GJ, Conklin DS: Short hairpin RNAs (shRNAs) induce sequence-specific silencing in...
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Báo cáo y học: "Identification of a truncated form of methionine sulfoxide reductase a expressed in mouse embryonic stem cells" pps
Ngày tải lên :
10/08/2014, 05:21
... conditions in mouse embryonic stem cells Methods Mouse embryonic stem cell culture The mouse embryonic stem (MES) cells (CCE-24) were routinely grown on 0.1% gelatin-coated dishes in Dubecco’s ... hindering the access for the long form protein, thus having an advantage to be evolutionarily selected and maintained Since the truncated protein itself does not contain the c-terminal cysteines, ... the mRNA level which is considered as a general initial response of stem cells to oxygen deprivation as it is observed in all Msr genes as well as in other genes such as matrix metalloproteinases...
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báo cáo khoa học: " Effects of DNMT1 silencing on malignant phenotype and methylated gene expression in cervical cancer cells" pdf
Ngày tải lên :
10/08/2014, 10:21
... required to maintain CpG methylation and aberrant gene silencing in human cancer cells Nature genetics 2002, 33(9):61-65 Suzuki M, Sunaga N, Shames DS, Toyooka S, Gazdar AF, Minna JD: RNA interference- mediated ... synergistically in inhibiting CpG island hypermethylation of tumor suppressor genes Rhee et al Figure Effects of DNMT1 silencing on gene methylation and mRNA expression of seven tumor suppressor genes in ... tumor-related genes in association with overexpression of DNA methyltransferase (DNMT1) during multistage carcinogenesis of the pancreas Carcinogenesis 2006, 27(6):1160-1168 Sowinska A, Jagodzinski PP: RNA...
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Báo cáo khoa hoc:" Shortcomings of short hairpin RNA-based transgenic RNA interference in mouse oocytes" ppsx
Ngày tải lên :
11/08/2014, 07:21
... would allow for using the same RNAi system for blocking genes in cultured cells or in tissues Second, cloning shRNA-producing vector is easier when compared to cloning large inverted repeats ... Lemming oocytes induced an efficient RNAi [32], suggesting that miRNA-like shRNA biogenesis is intact in rodent oocytes Here, we show that experiments with pol II-driven miRNA-like shRNA system ... silenced transgenes in all tissues in the F0 generation already In contrast, long hairpin RNAi transgenes produced by pronuclear microinjection in the same transgenic facility, in the same genetic...
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báo cáo khoa học: " Embryonic stem cells in scaffold-free threedimensional cell culture: osteogenic differentiation and bone generation" ppt
Ngày tải lên :
11/08/2014, 20:21
... cultivation in the presence of osteoinductive stimuli Numerous living cells were detected in the mineralized centre of the spheres by means of toluidine blue staining (Figure 1) Generally, the mineralization ... units up to 0.4 mm in diameter was established Feeder-independent murine embryonic stem cells (ESCs) were kindly provided by K Pfeffer (Institute for Microbiology, Heinrich Heine University of ... of stem cells into the osteogenic lineage in vitro J Bone Miner Res 2004, 19:1379-1394 Page of 38 Trounson A: Human embryonic stem cells: mother of all cell and tissue types Reprod Biomed Online...
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báo cáo khoa học:" Induction of osteogenic markers in differentially treated cultures of embryonic stem cells" ppt
Ngày tải lên :
12/08/2014, 01:22
... sources including autologous cells as well as allogenic and xenogenic cells [13-16] There are some reports that use totipotential embryonic stem cells in tissue engineering of bone [17,18] Embryonic ... BMP-2 Shown are von Kossa stainings with arrows pointing to the deposition of calcium phosphate-containing minerals that stained in black Figure LIF, stem cell PCR showing amplification of the without ... The increase in reaction products during PCR was monitored by measuring the increase in fluorescence intensity caused by the binding of SYBR green to doublestranded DNA that accumulated during...
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Báo cáo y học: "Identification and isolation of embryonic stem cells in reproductive endocrinology: theoretical protocols for conservation of human embryos derived from in vitro fertilization" ppsx
Ngày tải lên :
13/08/2014, 23:20
... Foundation, and Parkinson's Action Network, as well as the Cancer Research and Prevention Foundation This type of federal research funding strategy involving existing embryonic stem cell lines is consistent ... funding of research involving the more than 60 existing stem cell lines, but will not sanction or encourage destruction of additional human embryos Indeed, the embryos from which the existing ... Fischbach GD, Fischbach RL: Stem cells: science, policy, and ethics J Clin Invest 2004, 114:1364-1370 Donovan PJ, Gearhart J: The end of the beginning for pluripotent stem cells Nature 2001, 414:92-97...
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Báo cáo y học: "The impact of chromatin modifiers on the timing of locus replication in mouse embryonic stem cells" pot
Ngày tải lên :
14/08/2014, 08:20
... fibroblasts remained after 20-25 minutes plating in non-gelatinized plates Pre-plating of feeder-dependent ES cells in this way may result in a slight delay in the apparent time of replication for genes ... chromatin components In this regard, compensatory chromatin modifications have been previously described, including an increase in H3K27me3 levels in Suv39h1/h2 deficient ES cells [25] Minor and ... was seen in Dicer KO cells (17 compared with in matched wild-type cells) Increased minor and major satellite transcripts were also seen in Eed deficient ES cells but not in other mutant lines that...
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Báo cáo y học: "Exploring systemic RNA interference in insects: a genome-wide survey for RNAi genes in Tribolium" potx
Ngày tải lên :
14/08/2014, 08:20
... small RNAs are called short interfering RNAs (siRNAs) and are loaded into RNAinduced silencing complexes (RISC) for post-transcriptional silencing, or RNA- induced initiation of transcriptional gene ... amino-terminal DExH-Box helicase domains, a PAZ (Piwi/Argonaute/Zwille) domain, tandem RNase III domains and a carboxy-terminal dsRNA binding domain A single Dicer protein is involved in both the siRNA ... additional genes involved in the systemic RNAi response, including rsd-2, rsd-3, and rsd-6 [60] Mutants for these genes still retain the systemic RNAi response in somatic cells, but germ-line cells...
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