gap and gap associated proteins

Sterol rich membrane domains and membrane associated proteins in the fission yeast schizosaccharomyces pombe

Ngày tải lên : 15/09/2015, 17:09

... his lab and for his interest and support; my thesis committee members Suresh Jesuthasan, Naweed Naqvi and Thirumaran Thanabalu for their comments and suggestions; all lab members past and present ... membrane proteins; IMA/TLL facilities and staff for general support; National Science and Technology Board; Agency for Science, Technology and Research; Temasek Life Sciences Laboratory; Singapore ... shown to be membrane associated, to actomyosin ring assembly and maintenance at different stages of mitosis, we provide new insight into the roles of lipids and proteins and their relationship...

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Computational study of therapeutic targets and ADME associated proteins and application in drug design

Ngày tải lên : 15/09/2015, 22:19

... husband and my parents for their endless love, support, and encouragement I dedicate this thesis to them with all my love -I- Computational study of therapeutic targets and ADME -associated proteins ... druggable proteins 126 5.2.2 Prediction of druggable proteins by a statistical learning method.132 Computational analysis of drug ADME- associated proteins .137 6.1 ADME -associated proteins ... targets and ADME -associated proteins and application in drug design Acknowledgements ACKNOWLEDGEMENTS This thesis would not have been possible to be completed without the kind support, help, and...

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Báo cáo khoa học: cGMP and glutathione-conjugate transport in human erythrocytes The roles of the multidrug resistance-associated proteins, MRP1, MRP4 and MRP5 pot

Ngày tải lên : 23/03/2014, 21:20

... membrane vesicles shown in Fig The maximum uptake rates, Umax1 and Umax2, the dissociation constants, Kd1 and Kd2, and the Hill coefficients, n1 and n2 are defined as indicated in Eqn (1) The data were ... erythrocyte membrane vesicles The maximum uptake rates, Umax1 and Umax2, the dissociation constants, Kd1 and Kd2, and the Hill coefficients, n1 and n2 are as defined in Eqn (1) The data were collected ... MRP family Immunodetection of MRP4 and MRP5 proteins in human erythrocytes and COR-L23/R cells Fig Effect of cGMP on the DNP-SG transport in human erythrocytes and vice versa (Top) ATP-dependent...

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Báo cáo khoa học: Mechanical regulation of the Cyr61/CCN1 and CTGF/CCN2 proteins Implications in mechanical stress-associated pathologies pptx

Ngày tải lên : 30/03/2014, 10:20

... chondrocyte proliferation and extracellular matrix production and turnover [18,19] In this review we describe in vivo and in vitro evidence relating CTGF and Cyr61 to mechanical stress and discuss the ... expression in vitro and in vivo Mechanical regulation in bone and cartilage Cartilage and bone provide ideal tissues for the study of the mechanical regulation and function of Cyr61 and CTGF, because ... pressure and was associated with the induction of program cell death of mesangial cells [23] Thus, both in vivo and in vitro studies stress an important role of mechanical strain in the kidney and associated...

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Báo cáo y học: "erum levels of soluble receptor for advanced glycation end products and of S100 proteins are associated with inflammatory, autoantibody, and classical risk markers of joint and vascular damage in rheumatoid arthritis" doc

Ngày tải lên : 09/08/2014, 13:22

... (CV) events [19] Most S100 proteins have a mass between and 14 kDa, and are characterized by two calcium binding sites of the EF-hand type (helix-loop-helix) [20] S100A8 and S100A9, generally functioning ... relationship to joint and vascular damage and risk factors in RA patients [19] We report associations of each protein with both joint and vascular disease and their risk factors Materials and methods Subjects ... generally associated with a more favorable vascular risk profile in our RA cohort, and potentially associated with concomitant reduction in proinflammatory and/ or pro-atherogenic RAGE ligand binding...

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Study of the associated proteins of STAT3 and characterization of their functions roles of GRIM 19 and PIN1 in the regulation of STAT3 activity

Ngày tải lên : 15/09/2015, 17:10

... STUDY OF THE ASSOCIATED PROTEINS OF STAT3 AND CHARACTERIZATION OF THEIR FUNCTIONS: ROLES OF GRIM-19 AND PIN1 IN THE REGULATION OF STAT3 ACTIVITY LUFEI CHENGCHEN ... and patience throughout my whole candidature I am also grateful to the past and present members in my supervisory committee, Prof Alan Porter, Assoc Prof Uttam Surana, Prof Catherine Pallen and ... Pin1 and cdc25 in cell cycle progression 38 1.10.1.2 Pin1 and p53, p73 tumor suppressor 39 iii 1.10.2 Pin1 in Alzheimer’s disease 40 1.10.3 Pin1, cyclin D1 and breast cancer 41 1.10.3.1 Pin1 and...

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Báo cáo y học: "Weight loss, leukopenia and thrombocytopenia associated with sustained virologic response to Hepatitis C treatmen"

Ngày tải lên : 26/10/2012, 09:39

... schizophrenia (6%) and bipolar disorder (3%) Underlying medical problems included hypertension (15%) and diabetes mellitus (DM) (14%) Demographics, clinical and laboratory data of patients (percent and means) ... which has a limit of detection of 28 IU/mL Body weight measurement and laboratory data were collected and compared at the beginning and at the end of therapy The Knodell scoring system for liver ... laboratory data and liver biopsy results of all patients are shown in Table The major risk factors for acquiring HCV infection were related to injection drug use and tattooing Mild and moderate...

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Risks and Benefits Associated with Genetically Modified (GM) Plants

Ngày tải lên : 25/10/2013, 05:20

... Kaufman et al 13.2 Benefits and Risks of Genetically Modified Plants A number of positive and negative issues are associated with plant biotechnology and its applications (Ellstrand, 2000; Gadgil, 2000) ... Plants and Foods Derived from GM Plants: Questions and Answers The questions and answers cited below have been prepared by the World Health Organization (WHO) in response to questions and concerns ... biodiversity and wildlife; a decreased use of the important practice of crop rotation in certain local situations; and the movement of herbicide resistance genes to other plants 13 Risks and Benefits Associated...

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Control of Cell Proliferation and Growth byMyc Proteins

Ngày tải lên : 25/10/2013, 21:20

... adhesion to the basal lamina and Myc disrupts this adhesion; therefore, keratinocytes respond to Myc with premature differentiation and arrest of proliferation (Gandarillas and Watt 1997) The frequent ... in cell proliferation and their proposed functions Control of Cell Proliferation and Growth by Myc Proteins 333 2003) There is clear evidence that this effect is direct and not mediated by activation ... and Myc may repress a common set of target genes (Phan et al 2005) Mechanistically, many questions remain open Myc proteins interact with a number of potential co-activator and co-repressor proteins...

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Tài liệu Báo cáo khoa học: Interaction between very-KIND Ras guanine exchange factor and microtubule-associated protein 2, and its role in dendrite growth – structure and function of the second kinase noncatalytic C-lobe domain docx

Ngày tải lên : 14/02/2014, 19:20

... known to modulate polymerization, stability and rearrangement of microtubules in neuronal dendrites [13–15] and is associated with some neurological and psychiatric disorders [16,17] However, ... dendrites and soma, although not to axons The expression of three other KIND2-containing constructs (DKIND1, DRasN and DGEF) was restricted to dendrites and soma (Fig 1B) On the other hand, two ... CD2-1 region (Fig 3E) and examined the interactions between bacterially expressed proteins of these subregions (Fig S1C) and 1654 Fig Of all members of the KIND family of proteins, only KIND2...

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Báo cáo khoa học: Nuclear actin and actin-binding proteins in the regulation of transcription and gene expression docx

Ngày tải lên : 07/03/2014, 01:20

... family, ABLIM-2 and -3, as STARS-interacting proteins These novel proteins contain four LIM domains and a C-terminal villin headpiece domain, which mediates actin-binding in several proteins, such ... a-actinin-2 ()) Bundling proteins Coactivator Supervillin NLS F-actin- and membraneassociated scaffolding protein Filamin NLS? Cross-linking proteins Filamin A NLS? Cross-linking proteins Transgelin ... (e.g from CBP and p300) and methylating (e.g from CARM1 and protein arginine methyltransferase 1) activities of the p160 coactivator complex [109] In addition, Fli-I and Fli-I LRR -associated protein...

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Báo cáo khoa học: Two overlapping antiparallel genes encoding the iron regulator DmdR1 and the Adm proteins control sidephore and antibiotic biosynthesis in Streptomyces coelicolor A3(2) pdf

Ngày tải lên : 07/03/2014, 02:20

... behaviour of TAadm and TAdmdR1 strains regarding production of antibiotic suggests interaction between the actions of the Adm and DmdR1 proteins (see below) Lack of both DmdR1 and Adm proteins prevents ... formation of desferrioxamines B and E (labelled as DesB and DesE) and complementation of desferrioxamine production (A) Cultures of S coelicolor A3(2) and the TAdmdR1, TAadm and DdmdR1 ⁄ adm mutants ... genes and iron regulation A B C Fig Immunodetection of DmdR1 and Adm proteins (A,B) Western blot reactions of wild-type S coelicolor A3(2) (lane 2) and the TAadm (lane 3), TAdmdR1 (lane 4) and...

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Báo cáo khoa học: Thermodynamic stability and folding of proteins from hyperthermophilic organisms doc

Ngày tải lên : 07/03/2014, 05:20

... (a) co-chaperonin proteins, (b) nonheme iron proteins, (c) DNA-binding proteins, as well as (d) a few other proteins Although the number of characterized hyperthermostable proteins is rather ... kinetic folding ⁄ unfolding parameters (kf and ku) for hyperthermostable proteins If not otherwise stated, TM and DGU refer to pH 7, and kf ⁄ ku to pH and 20–25 °C, conditions In the last column, ... hyperthermostable proteins were included Perhaps proteins from hyperthermophilic organisms most often use Models and 2, whereas thermostable proteins are more likely to use Models and It was recently...

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SPECIAL STUDY: INJURIES AND DEATHS ASSOCIATED WITH CHILDREN’S PLAYGROUND EQUIPMENT doc

Ngày tải lên : 08/03/2014, 13:20

... 1981 2-volume handbook set for public playground safety with a single Handbook for Public Playground Safety.(5,6) The 1991 handbook was then revised and republished in 1997 The new Handbook is intended ... years, and 0.6 per 10,000 population 15 years and older The design and use of playground equipment may affect associated patterns of injury Based on this study, 22.8 percent of the equipment associated ... Diagnosis, and Disposition Injuries to the arm and hand (primarily the lower arm, wrist, and elbow) were most common, with 43 percent of the injuries (Table 3) Injuries to the head and face followed,...

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Báo cáo khóa học: C-, 15N- and 31P-NMR studies of oxidized and reduced low molecular mass thioredoxin reductase and some mutant proteins docx

Ngày tải lên : 16/03/2014, 16:20

... template and the oligonucleotides FAD(CA)-1 and FAD(CA)-2 as primers (Table 1) The 1006-bp amplication product was digested with EcoRI and BamHI and ligated into the vector pMal-c2 (New England Biolabs) ... TARFH2 and FMNH2, are C(5a), C(7), C(9a) and C(10a), and as already mentioned above, C(2) (Fig 7B) With respect to the 13C chemical shifts of FMNH2, C(2), C(5a), C(9a) and C(10a) of wild-type and ... site disulde is close to and can oxidize the avin (FO), and the form in which the nascent dithiol is exposed to solvent and the pyridine nucleotide binding site is close to and can reduce the avin...

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Báo cáo khoa học: Functional interplay between viral and cellular SR proteins in control of post-transcriptional gene regulation pptx

Ngày tải lên : 23/03/2014, 06:20

... host and virus via their RS domain-containing proteins Virus-encoded SR proteins cutaneous and mucosal lesions, ranging from warts to neoplasia and even cancer [14] A subset of HPV types are associated ... cycle, including positive-strand and minus-strand DNA synthesis, pre-genomic RNA packaging, and virion formation and release [17,18] This core protein is a phosphoprotein, and its function may be ... the interplay between viral proteins and cellular SR proteins (A) Viral SR proteins (rectangle) recruit cellular SR proteins (oval) to promote splicing efﬁciency and ⁄ or modulate alternative...

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Báo cáo Y học: Supermolecular organization of photosystem II and its associated light-harvesting antenna in Arabidopsis thaliana docx

Ngày tải lên : 24/03/2014, 04:21

... (A) and (B), arrows point to the site of strongest difference (D) Image form (A) with contours; positions of S-type and M-type LHCII trimers indicated in yellow and positions of CP29, CP26 and ... position, with the dimeric part (‘c’), the S-type and M-type LHCII trimers (‘s and ‘m’) indicated in yellow, the CP29, CP26 and CP24 areas in green and the strongest difference area from image (C) ... to E J B and J P D and from the Danish National Research Foundation to P E J and H V S is gratefully acknowledged REFERENCES Hankamer, B., Barber, J & Boekema, E.J (1997) Structure and membrane...

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Báo cáo khoa học: Role of phosphorylation in p53 acetylation and PAb421 epitope recognition in baculoviral and mammalian expressed proteins pdf

Ngày tải lên : 30/03/2014, 16:20

... in which proteins are expressed and post-translationally modified [8] Experimental procedures Baculoviral expression and purification of p53 proteins S37D S376A S376D S378A S378D Fig p53 proteins ... human and murine, and also on a dimeric mutant L J Warnock et al PAb240 Mouse WT S15P Dimer Fig Recombinant human and murine wild-type p53 proteins are phosphorylated at S15 and acetylated at ... K373Ac K373/K382Ac 10 11 12 13 Fig Phosphorylation status at S15 and acetylation status at K373 and K382 of murine wild-type p53 and p53 proteins mutated at specific phosphorylation sites expressed...

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Báo cáo khoa học: A dimer of the FeS cluster biosynthesis protein IscA from cyanobacteria binds a [2Fe2S] cluster between two protomers and transfers it to [2Fe2S] and [4Fe4S] apo proteins ppt

Ngày tải lên : 31/03/2014, 01:20

... acid and 25% acetonitrile was used as matrix Determination of sulfide and iron Determination of sulfide and iron were carried out as described by Siegel [30] and Fish [31] Gel electrophoresis and ... recombinant IscA1 and soluble protein extract from Synechocystis Lanes 1, and 3: 7.5, 15 and 30 ng purified recombinant IscA1, respectively; lane 4: 60 lg soluble Synechocystis proteins DNA sequence ... Ollagnierde-Choudens et al [34] and Krebs et al [35] who also observed an assembly of an FeS cluster at IscA from E coli and A vinelandii, respectively In contrast with A vinelandii Nif IscA we could...

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Báo cáo hóa học: " MMP-1 is a (pre-)invasive factor in Barrettassociated esophageal adenocarcinomas and is associated with positive lymph node status" doc

Ngày tải lên : 18/06/2014, 16:20

... into collagenases (MMP-1, -8, and -13), gelatinases (MMP-2 and 9), stromelysins (MMP-3 and 10), matrilysins (MMP-7 and 26), and the membranetype MMPs (MMP-14 to 17 and 24) Furthermore MMPs are ... expression is associated with BE and associated EAC MMP-1, MMP-13 and Cdx-2 were not expressed in normal esophageal squamous epithelium MMP-1 expression in stromal cells was considerably weak and strongly ... Glyceraldehyde-3phosphate dehydrogenase (GAPDH) was used for relative quantification and cDNA quality control The GAPDH forward primer sequence was: 5′-ATCCCATCACCATCTTCCAGG-3′; the GAPDH reverse primer sequence...

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