fundamentals of producing lung cells siti norhayati ismail julia m polak and anastasios mantalaris
Báo cáo y học: "TNF inhibits production of stromal cell-derived factor 1 by bone stromal cells and increases osteoclast precursor mobilization from bone marrow to peripheral blood" ppt
Ngày tải lên :
09/08/2014, 10:23
... chamber of a transwell with or without 100 ng/ml SDF-1 in the lower chamber for hours Nonmigrated cells from the upper chamber and migrated cells from the lower chamber were cultured with M- CSF ... Lapidot T: Mutual, reciprocal SDF-1/CXCR4 interactions between hematopoietic and bone marrow stromal cells regulate human stem cell migration and development in NOD/SCID chimeric mice Exp Hematol ... expression of CD11b and Gr-1 proteins, cell surface markers for OCPs [20], with primary bone marrow mononuclear cells isolated from the same mice As we reported previously [20], more than 10% of primary...
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Báo cáo y học: "Abnormal networks of immune response-related molecules in bone marrow cells from patients with rheumatoid arthritis as revealed by DNA microarray analysis" ppsx
Ngày tải lên :
12/08/2014, 17:21
... Salazosulfapyridine 1000 mg/day, MTX mg/week MTX mg/week 17 40 2.9 MTX mg/week CRP, C-reactive protein; DMARDs, disease-modifying antirheumatic drugs; MTX, methotrexate; RA, rheumatoid arthritis; RF, rheumatoid ... produced from HSCs in the BM, the s abnormality in immune response and suppression of cell cycle in BM may contribute to the pathogenesis of RA Conclusions BM cells from RA patients had abnormal functional ... accumulating evidence suggesting that abnormalities in the bone marrow (BM) have a significant role in RA inflammation [2,8,9] The BM contains three types of stem cells: hematopoietic stem cells...
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Báo cáo khoa học: "Radioprotective effects of an acidic polysaccharide of Panax ginseng on bone marrow cells" pdf
Ngày tải lên :
07/08/2014, 20:23
... gnitsevrah yb denimreted saw sCD fo noitroporp ehT syad rof FSC-MG esuom tnanibmocer lm/gn 01 fo ecneserp eht ni derutluc erew sMB detsevrah ehT srumef morf DS ± naem eht dna ecim detaidarri ro ... dohtem eht yb deifirup saw GPA erac dna esu lamina yrotarobal rof )ASU( senilediug HIN eht htiw ecnadrocca ni demrofrep erew stnemirepxe lamina ehT stnemirepxe eht rof desu erew ecim elamef dlo-keew ... eht gnisu detareneg erew )sCDMB( sllec citirdned devired -worram enoB )ASU ,negortivnI( nicymotperts/nillicinep lm /U 001 ,enimatulg-L Mm ,SBF gniniatnoc muidem IMPR gnisu derutluc erew sllec...
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Báo cáo khoa học: "Radioprotective effects of fucoidan on bone marrow cells: improvement of the cell survival and immunoreactivity" doc
Ngày tải lên :
07/08/2014, 23:22
... measurement of cell viability BMCs were harvested from the femurs and tibias of mice of C57BL/6 mice as described in previous report [9] Any contaminated red blood cells were eliminated by ammonium ... proliferation of BMCs themselves in total value, all BMCs were irradiated immediately prior to co-culture The culture medium was RPMI 1640 medium containing 10% FBS, 0.1 mM non-essential amino acid, mM sodium ... precursors or matured cells Using MLR assays, BMCs of C57BL/6 mice were used as stimulators and splenocytes of Balb/c mice were used as allogeneic responder cells To measure the proliferation of allogeneic...
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Báo cáo y học: "Quantitative ultrasound can assess the regeneration process of tissue-engineered cartilage using a complex between adherent bone marrow cells and a three-dimensional scaffold" docx
Ngày tải lên :
09/08/2014, 06:22
... containing 15 ml of medium The medium used was Eagle's minimal essential medium (MEM) containing 10% fetal bovine serum and antibiotics (penicillin, 100 U/ml; streptomycin, 0.1 mg/ml; and amphotericin ... center and four points at mm above, below, left, and right of the center The percentage maximum magnitude (the maximum magnitude of the measurement area of the operated knee divided by that of the ... right The maximum magnitude is indicated by the gray scale and the percentage maximum magnitude (the maximum magnitude of the measurement area divided by that of the intact cartilage of the nonoperated...
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Báo cáo y học: " Reconstitution of the myeloid and lymphoid compartments after the transplantation of autologous and genetically modified CD34+ bone marrow cells, following gamma irradiation in cynomolgus macaques" potx
Ngày tải lên :
13/08/2014, 05:21
... on a layer of stromal cells of the murine fibroblastic cell line M2 -10B4, in a medium composed of αMEM supplemented with 12.5% horse serum (HS), 12.5% FBS, mM L-glutamine, 10-4 M 2-mercaptoethanol, ... animals of groups and in terms of the minimum number of cells (821 ± 226 cells/ μl for group and 658 ± 107 cells/ μl for group 2, P = 0.3768 (Mann & Whitney test)) or the time at which that minimum ... fetal bovine serum, 10-4 M 2mercaptoethanol, mM L-glutamine, 50 ng/ml rhSCF, 10 ng/ml rhGM-CSF, 10 ng/ml rhIL-3 and IU/ml rhEPO Cells were cultured at a density of 104 cells/ ml (in triplicate)...
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báo cáo hóa học: " Comparison of immature and mature bone marrow-derived dendritic cells by atomic force microscopy" potx
Ngày tải lên :
21/06/2014, 02:20
... size of × m and the mature BMDCs (L) in the scanning size of × m; F and M, Enlarged view of the center of the immature BMDCs (F) and the mature BMDCs (M) in the same scanning area of × m; G and ... Therefore, a comparative study of immature and immature BMDCs was carried out by AFM to Figure MHC-II expression and IL-12 production of immature and mature BMDCs (A-D) Flow cytometry was used ... induction of rmGM-CSF plus rmIL-4, the BMDCs appeared predominately round in loosely adhesive Figure Morphologic changes of immature and mature BMDCs (A, B) The morphology of the BMDCs treated with GM-CSF...
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Augmentation of neovascularization in murine hindlimb ischemia by combined therapy with simvastatin and bone marrow-derived mesenchymal stem cells transplantation pptx
Ngày tải lên :
10/08/2014, 05:21
... R, Mosca JD, Moorman MA, Simonetti DW, Craig S, Marshak DR: Multilineage potential of adult human mesenchymal stem cells Science 1999, 284:143-147 Dimmeler S, Leri A: Aging and disease as modifiers ... nonischemic limb Simvastatin administration and MSCs transplantation Simvastatin administration and MSCs transplantation were performed immediately after hindlimb ischemia was created Simvastatin ... simvastatin + Figure Effect of simvastatin and bone marrow-derived MSCs administration on angiogenesis in ischemic limb A Representative microphotographs of the section of ischemic hindlimb muscles...
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báo cáo khoa học: "Optimized labeling of bone marrow mesenchymal cells with superparamagnetic iron oxide nanoparticles and in vivo visualization by magnetic resonance imaging" ppsx
Ngày tải lên :
11/08/2014, 00:22
... to accommodate rats All other experiments were performed on rats 2.1 Isolation and Cultivation of Rat/Mouse Mesenchymal Cells from Bone Marrow To obtain bone marrow cells, tibias and femurs were ... alcohols and mounted with Entellan (Merck KGaA, Darmstadt, Germany, http://www.merck.de) Samples were observed by light microscopy 2.5 Immunocytochemistry For immunofluorescence, MSCs were grown and ... biological properties of human mesenchymal stem cells NMR Biomed 2010, 23:514-522 59 Modo M, Mellodew K, Cash D, Fraser SE, Meade TJ, Price J, Williams SC: Mapping transplanted stem cell migration after...
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Báo cáo y học: "ntravenous transplantation of allogeneic bone marrow mesenchymal stem cells and its directional migration to the necrotic femoral head"
Ngày tải lên :
25/10/2012, 11:18
... al Mesenchymal stem cells: mechanisms of immunomodulation and homing Cell Transplant 2010;19(6):667-79 Mouiseddine M, François S, Semont A, et al Human mesenchymal stem cells home specifically ... and in vivo infusion of bone marrow-derived Flk-1+CD31-CD34- mesenchymal stem cells: feasibility and safety from monkey to human Stem Cells Dev,2006,15(3):349-357 Devine SM, Bartholomew AM, Mahmud ... nude mice and the distribution and migration of MSCs were dynamically monitored to evaluate the feasibility and safety of intravenous implantation of allogeneic MSCs in the treatment of femoral...
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báo cáo hóa học:" High correlation of the proteome patterns in bone marrow and peripheral blood blast cells in patients with acute myeloid leukemia" pot
Ngày tải lên :
18/06/2014, 15:20
... #02-36 #02-39 #02-38 60 Female M2 t(8;21) 22 Female M2 normal 63 Female M5 b normal 46 Male M1 complex 27 Female M0 t(9;11) 58 Male M4 normal PB BM PB BM PB BM PB BM PB BM PB BM 4.8 (80%) * 379.0 (93%) ... thesize most components of the plasminogen activation system Among the numerous features shared by normal monocytes and M4 cells were the capability to migrate to areas of inflammation and to ... (RCN) RCN is a member of the EF-hand Ca(2+)-binding protein family and may regulate calciumdependent activities in the endoplasmatic reticulum lumen or post-ER compartment TCHTP Cytoplasmatic Ca2(+)-binding...
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báo cáo hóa học:" A practical approach for the validation of sterility, endotoxin and potency testing of bone marrow mononucleated cells used in cardiac regeneration in compliance with good manufacturing practice" docx
Ngày tải lên :
18/06/2014, 15:20
... 996:235-244 Pittenger MF, Mackay AM, Beck SC, Jaiswal RK, Douglas R, Mosca JD, Moorman MA, Simonetti DW, Craig S, Marshak DR: Multilineage potential of adult human mesenchymal stem cells Science 1999, ... transplantation of bonemarrow cells: a pilot study and a randomised controlled trial Lancet 2002, 360(9331):427-435 Miyamoto K, Nishigami K, Nagaya N, Akutsu K, Chiku M, Kamei M, Soma T, Miyata S, Higashi M, ... efficiency of the intra-arterial administration of BMC into an ischemic limb [13,14] During the production of the BM-MNC as medicinal products, variable amounts of impurities product and process-related,...
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Báo cáo hóa học: "Hybrid approach of ventricular assist device and autologous bone marrow stem cells implantation in end-stage ischemic heart failure enhances myocardial reperfusion" doc
Ngày tải lên :
18/06/2014, 16:20
... Kukucka M, Dandel M, Knosalla C, Potapov E, Lehmkuhl HB, Meyer R, Ebell W, Stamm C, Hetzer R: Intramyocardial delivery of bone marrow mononuclear cells and mechanical assist device implantation ... N, Matsuura N, Myoui A, Matsuyama A, Ezoe S, Iida H, Matsuda H, Sawa Y: Combined autologous cellular cardiomyoplasty using skeletal myoblasts and bone marrow cells for human ischemic cardiomyopathy ... nonischemic cardiomyopathy, the degree of cardiac recovery is limited in patients with ICM Implantation of stem cells to promote myocardial perfusion during mechanical support in end-stage ICM may eventually...
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báo cáo hóa học:" Biocompatibility of Poly-ε-caprolactone-hydroxyapatite composite on mouse bone marrow-derived osteoblasts and endothelial cells" doc
Ngày tải lên :
20/06/2014, 01:20
... by dexamethasone Endocrinology 1994, 134:277-286 Mikos AG, Sarakinos G, Vacanti JP, Langer RS, Cima LG: Biocompatible polymer membranes and methods of preparation of three dimensional membrane ... (Invitrogen, US), 10 mM β-glycerol phosphate (Sigma-Aldrich, US), 10-4 M L-ascorbic acid (Sigma-Aldrich, US), and 10 nM dexamethasone (Sigma-Aldrich, US), mM glutamine (Invitrogen, US), 100 U/ml penicillin ... bone marrow-derived osteogenic and endothelial cells Methods Cell Culture and Induction Bone marrow cells were obtained from male BALB/c mice (6–8 weeks of age) Low-density bone marrow mononuclear...
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Báo cáo y học: "Osteoclast-independent bone resorption by fibroblast-like cells" doc
Ngày tải lên :
09/08/2014, 01:21
... stain human cells of mesenchymal origin but specifically not detect vimentin in mouse cells [22] Immunohistochemistry showed the presence of vimentin-positive human PLFs in all samples, with most ... concentration between and 300 ng/ml; Roche Biochemicals, Basel, Switzerland), ionomycin (10 µg/ml; Biomol, Hamburg, Germany) or control medium For the ionomycin measurements, dimethylsulfoxide was ... release of H+, cells were treated by cyclic influx of medium containing amiloride (Calbiochem, Bad Soden, Germany) at concentrations between 100 µmol/l and mmol/l, bafilomycin A1 (Calbiochem) at...
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Báo cáo y học: "Bone marrow lesions from osteoarthritis knees are characterized by sclerotic bone that is less well mineralized" ppsx
Ngày tải lên :
09/08/2014, 01:22
... taken from the BML area, from another area within the medial tibiofemoral compartment not affected by BML, and from the lateral tibiofemoral compartment as well as from matched locations from the ... tibiofemoral compartment not affected by BMLs, and from the lateral tibiofemoral compartment as well as from matched locations from the lateral compartment were defined (c) Multiple cores were machined ... milliseconds/15 milliseconds, 60 milliseconds, 2.5 to mm slices, no skip/gap, 256 × 256 matrix, 12 cm field of view (for distal femur and proximal tibia), and acquisition time 4.50 minutes Images from the MRI...
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Báo cáo y học: "Transcriptional profiles discriminate bone marrow-derived and synovium-derived mesenchymal stem cells" ppsx
Ngày tải lên :
09/08/2014, 07:20
... dexamethasone (Sigma, l'Isle d'Abeau, France), 0.17 mM ascorbic acid and 1% insulin-transferrin-sodium selenite media supplement (Sigma) The osteogenic medium consisted of DMEM medium supplemented ... and immunologic properties of differentiated and undifferentiated mesenchymal stem cells Exp Hematol 2003, 31:890-896 Mourad W, Mehindate K, Schall TJ, McColl SR: Engagement of major histocompatibility ... obtained with cells from BM (estimated to be in 104 or 105 mononuclear cells) [29] Alternatively, they may reflect a commitment of stem cells under the influence of the environmental parameters The...
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Báo cáo y học: "Enhanced expression of mRNA for nuclear factor κB1 (p50) in CD34+ cells of the bone marrow in rheumatoid arthritis" potx
Ngày tải lên :
09/08/2014, 07:20
... SCF, GM-CSF and TNF-α Of note, silencing of NFκB1 also significantly decreased the ratios of MMP-1 and VEGF to β2MG (MMP-1/β2MG and VEGF/β2MG) in culture supernatants of RA bone marrow CD34+ cells ... various components of NFκB in bone marrow CD34+ cells in RA Materials and methods Patients and samples Bone marrow samples were obtained from 49 patients with RA (8 males and 41 females: mean age, ... human MMP-1 ELISA system (Amarsham Pharmacia Biotech, Buckinghamshire, UK) and human VEGF immunoassay kit (BioSource International, Camarillo, CA, USA), respectively The concentrations of β2-microglobulin...
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