functionalized nanoparticles for cell tracking

... attached cells per mm2 yielded 147 cells/ mm2 for the negative control, 1681 cells/mm for the positive control, and 1253 cells/mm for the Sundew adhesive coated surface Due to the non-uniformity ... the Sundew network was capable of supporting cell growth To test this ability, PC12 cells were chosen as a model system for nerve cell growth PC12 cells were derived from a pheochromocytoma of ... 2.5% fetal bovine serum at a density of × 10 cells/cm The cells were then incubated on the samples for 24 hours in a 37°C incubator with 5% CO2 to allow for attachment After 24 hours, the samples...

Ngày tải lên: 11/08/2014, 00:22

... Fe-PNs, and PtFe-PNs against UMUC3 bladder cancer cells Cells were exposed to the drug or nanoparticles for h and further cultured with fresh medium for 72 h                                       ... potential for various biomedical applications, including MRI for cell targeting and monitoring, drug/gene delivery, hyperthermia for cancer treatment, etc In addition, in contrast to other inorganic nanoparticles, ... resulting core-shell nanoparticles were further functionalized with an antibody for ultra-sensitive targeted detection by MRI and these nanoparticles showed synergistic effect for the inhibition...

Ngày tải lên: 09/09/2015, 10:14

... Superparamagnetic nanoparticles 19 2.3.1 Properties of superparamagnetic nanoparticles 21 2.3.2 Synthesis of superparamagnetic nanoparticles 22 2.3.3 Surface modification of magnetic nanoparticles ... (en:-NHCH2CH2NH2) functionalized magnetic nanoparticles are fabricated by grafting mono-6-ethylenediamino-6-deoxy-β-CD (β-CDen) onto thiodiglycolic acid (TDGA) modified magnetic nanoparticles For confirmation ... of silica and CMCD coating on bare magnetic nanoparticles 80 Figure 3-2 Preparation steps for fabricating β-CDen functionalized magnetic nanoparticles 83 Figure 4-1...

Ngày tải lên: 09/09/2015, 17:57

... carrying the foreign MHC The CD4+ cells help to stimulate the response of B cells and CD8+ cells which on identifying the foreign cells, reorganise their cytoskeleton at the T cell/ target cell interface ... stem cells and multipotent adult stem cells The pluripotent embryonic stem cells from the inner cell mass can differentiate into any cell in the body In comparison, the multipotent stem cells ... “colony forming unit – fibroblast” (CFU-F), for their ability form colonies of fibroblast-like cells The nomenclature developed from CFU-F to multipotent stromal cells or mesenchymal stem cells,...

Ngày tải lên: 14/09/2015, 08:49

... a MF for and after incubation in PBS for 24 h, (c) and (f) with addition of CMCS-MNPs (2.0 mg/mL) with MF for and after incubation in PBS for 24 h Figure 4-10 Viability of 3T3 fibroblast cells ... to rapid vortex mixing for 20 s Serial ten-fold dilutions were performed and the number of bacteria cells was counted using the spread plate method For the planktonic cells experiments, 0.1 mL ... the cells in biofilms grow through a combination of cell division and recruitment The formation of a biofilm ended with the last step known as development, which may result in an aggregate cell...

Ngày tải lên: 02/10/2015, 17:14

... Dynamic Separation of Duty Relations Conformance • Standard provides for conformance by vendor self-declaration • Standard provides foundation for thirdparty conformance testing sought by vendors ... Need for consistent, universally understood semantics for RBAC • Vendors value “establishing a taxonomy and a shared vocabulary for us, our customers, and the industry as a whole” Is RBAC ready for ... can be combined into relevant packages giving: – IT consumers a basis for uniform acquisition specification and a basis for making purchasing decisions – Vendors a set of benchmarks use in the...

Ngày tải lên: 17/02/2014, 15:20

... (f) staining cells Scale bars = 200 lm (F) Quantification of binucleated cells in control and Grx3-KD cells Total 600 cells counted for control cells and 360 cells counted for Grx3-KD cells The bars ... to finish cell cycle back to G1 phase Cells were harvested at 2-h intervals Half of cells were stained with propidium iodide, and analyzed by FACS Another half of cells were prepared for cell lysate ... °C for The cells were washed three times with NaCl ⁄ Pi for each, and then blocked in 10% normal goat serum for h at room temperature The cells were incubated in mAb against b-actin (1 : 100) for...

Ngày tải lên: 14/03/2014, 23:20

... chosen for hCypA labelling were 50 lM for [15N]Trp and [15N]Tyr, 150 lM for [15N]Ile, [15N]Thr and [15N]His, 0.35 mM for remaining Group II [15N]amino acids, and mM for those in Group III The S30 cell ... Value for Saccharomyces carlsbergensis, no data available for E coli; b value for Lupinus luteus and bovine, no data available for E coli; c value for Paracoccus denitrificans, no data available for ... evidence for more than a single conformation of His70, neither at pH 7.5 nor at pH 6.5 Discussion In this study, several aspects of a cell- free protein synthesis system based on an E coli cell extract...

Ngày tải lên: 16/03/2014, 18:20

... number of clusters k for the k-means algorithm is not given beforehand We thus use the Bayesian Information Criterion (BIC) as the splitting criterion, and select the proper number for k Related Work ... previously may be extracted This is a rich space for further exploration The effect of story pairs for the tracking task also depends on the performance of bilingual term correspondences We obtained ... are not included in the EDR bilingual dictionary For example, ’ エンデバー (Endeavour)’ which is a key term for the topic ‘Shuttle Endeavour mission for space station’ from the TDT3 corpus is not included...

Ngày tải lên: 17/03/2014, 04:20

... smoothly, with a speed profile that is sensitive to the user’s performance Also the assistive force is modulated by the tracking performance Due to the fact that the task is intrinsically continuous ... constant level of assistive force throughout sessions would progressively depress voluntary control instead of promoting it An approach for accounting systematically for these problems may be called ... vertical LCD screen for displaying target and hand position, while the arm motion occurs in the horizontal plane, might be a problem for the patients We could rule out this possibility, for the studied...

Ngày tải lên: 19/06/2014, 08:20

... imaging system Cell viability assays A 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was performed to determine cell viability HeLa cells were chosen for the cell culture ... superparamagnetic nanoparticles for targeted cellular uptake and detection by MRI J Biomed Mater Res 2006, 78:550 Kim SH, Jeong JH, Chun KW, Park TG: Target-specific cellular uptake of PLGA nanoparticles ... 360 nm In vitro cellular uptake of different kinds of NPs Human cervical carcinoma (HeLa) cell lines were provided by the Shanghai Institutes for Biological Sciences, and the cells were grown...

Ngày tải lên: 20/06/2014, 22:20

... of the nanoparticles It appears that the capacity of electrodes containing villiform-type Co3O4 nanoparticles is superior to that of electrodes containing cube- and flower-type Co3O4 nanoparticles ... flower-type Co3O4 nanoparticles were just about 1,800 mA h·g−1 although they have a shape very similar to the villiform-type Co3O4 nanoparticles As shown in Figure 3b, the cyclic performance of the ... the air electrodes before testing, the Co3O4 nanoparticles and carbon (Ketjen black) could be clearly identified (Figure 4) It was noticeable that the villiform-type Co3O4 nanoparticles maintained...

Ngày tải lên: 20/06/2014, 23:20

... internalization for the two forms of Cu Free copper would be taken up by the cell via normal metal trafficking pathways that utilize metal-binding proteins located on the cell surface As the cell begins ... in vitro toxicity in cancer cells Page of 10 Results Synthesis and characterization of Cu-loaded polymeric nanoparticles Carboxylate -functionalized, acrylate-based nanoparticles were synthesized ... have therefore designed a carboxylate -functionalized, polymer-based nanoparticle capable of sequestering a prototypical metal, Cu2+, for the ultimate goal of delivering Cu2+ to cancer cells to...

Ngày tải lên: 21/06/2014, 02:20

... adenocarcinoma cell line MCF-7 cells (passage 30–35, American Type Culture Collection) and their uptake of the coumarin-6-loaded nanoparticles were performed in the same way Caco-2 cells were re-seeded ... Fluoview FV500 In Vitro Cell Viability MCF-7 cells were seeded in 96-well plates at the density of 5,000 viable cells per well and incubated 24 h to allow cell attachment The cells were incubated ... same amount of nanoparticles for 24, 48 and 72 h, respectively At determined time, the formulations were replaced with DMEM containing MTT (5 mg/ml) and cells were then incubated for additional...

Ngày tải lên: 21/06/2014, 08:20

... the former is a qualitative measure of tracking performance while the latter is a quantitative measure, based on an error measure as in Section 4.2, for instance The drift measure for vehicle tracking ... of tracking tasks: head tracking via active contours [22, 23], edge and color histogram tracking [24, 25], sonar [26], and phase [27] tracking, to name few Particle filters have also been used for ... algorithms For this reason, particle filtering techniques for visual tracking have been developed only recently with the widespread of powerful computers Particle filters for visual object tracking...

Ngày tải lên: 21/06/2014, 08:20

... 2D tracking of each view to obtain real-time 3D tracking Hu et al [15] proposed a framework for tracking multiple people about uncalibrated occlusion reasoning Khan and Shah [16] presented a tracking ... computed using (22) For evaluation, three sequences, for which sample images are shown in the three rows of Table 1, were used for the test The 3D tracking results obtained for the person wearing ... pair Every two views form a camera pair and are applied to a local estimator for observations The direct linear transform (DLT) [27] is adopted as the reconstruction method for each camera pair...

Ngày tải lên: 21/06/2014, 16:20

... flower-type Co3O4 nanoparticles were just about 1, 800 mA h·g -1 although they have a shape very similar to the villiform-type Co3O4 nanoparticles As shown in Figure 3b, the cyclic performance of the ... the air electrodes before testing, the Co3O4 nanoparticles and carbon (Ketjen black) could be clearly identified (Figure 4) It was noticeable that the villiform-type Co3O4 nanoparticles maintained ... explanation for the higher discharge capacity of the air electrode containing villiform-type Co3O4 nanoparticles in comparison with the air electrode containing other types Co3O4 nanoparticles...

Ngày tải lên: 21/06/2014, 17:20

... because of the need to account for the kinds of requirements described in this paper, which imply that foresight and planning are crucial for good long-term performance In the context of UAV ... transition law takes the following form: xk+1 ∼ pk (· | xk ) (1) for some time-varying distribution pk However, the model for the UAV guidance problem constrains the form of the state transition law ... should perform well in our tracking application as long as the target motion is reasonably predictable with the tracking model within the chosen planning horizon The general procedure for using...

Ngày tải lên: 21/06/2014, 22:20

... to be done specifically for each one, and of course for the uplink and the downlink Therefore the formulation explained previously, and the value R p , has to be applied for each service i and ... has to be done also for the uplink direction (UL) Therefore, at the end we have obtained another set of two vectors (one for the uplink and one for the downlink), with the cell radius by capacity ... LTotal i are LTotal = 0.105 for the service S1 , voice, LTotal i = 0.271 for S2 , data 64 Kbps and LTotal i = 0.373 for S3 , data 144 Kbps The results for the downlink cell radius per traffic are...

Ngày tải lên: 21/06/2014, 23:20

... serum for endothelial cell lines (Sigma) supplemented with 10% FBS (Invitrogen) and endothelial cell attachment factor (E 9765) (Sigma) at 37 °C, 5% CO2 For the investigation of cytotoxicity of nanoparticles, ... is a marker of cell viability, and the other is a marker of cytotoxicity The live -cell protease activity is restricted to intact viable cells and is measured using a fluorogenic, cell- permeant, ... 19], which make MSNs an ideal nonviral carrier for gene, and/or drug delivery For instance, Lin and Wang utilized the MSNs with honeycomb structure for delivering DNA and chemicals into plants...

Ngày tải lên: 22/06/2014, 00:20