functionalization of surfaces with synthetic oligonucleotides

... were washed twice with buffer C and twice with buffer C without NP40 The elution was made with three volumes of buffer C without NP40 adjusted to 1.5 m NaCl, incubating for 10 with gentle stirring ... nuclear-enriched fraction of proteins The efficiency of labelling of the single oligomers being not homogenous, the ratios of the different binding signals within each lane instead of their absolute values ... the amount of recovered protein owing to the presence of a high molar excess of BSA remaining in the recovery buffer Therefore, a fixed aliquot of the protein was incubated with ng of the indicated...

Ngày tải lên: 16/03/2014, 13:20

... islands with the STM and examined the quality of STM images of the islands Note that after the creation of islands with the STM, the accumulation of silicon atoms on the tip apex was not observed with ... The scattering of the island height in one experimental run increased with increasing tunneling current The increase of the scattering coincided with the appearance of fluctuations of the tunneling ... islands, grown with the STM, have a high aspect ratio (height divided by base length) of about 0.3 [7] We shall assume that all islands are cone-shaped with an aspect ratio of 0.3 The amount of silicon...

Ngày tải lên: 16/03/2014, 15:32

... turn to the proof of Theorem 1.4 Let Ᏼk be the space of surface spherical harmonics of degree k on Sn−1 with dimension Dk By the same argument as in [3], one can reduce the proof of Theorem 1.4 ... denotes the area of S Dk ∞ j =1 Nt (ξ) Yk, j (ξ ) dt t ≤ Ck −2 Thus, inequality (2.15) holds and the proof of Theorem 1.4 is finished Proof of Theorem 1.7 We will give the proof of Theorem 1.7 ... independent of f On the other hand, as a related vector-valued singular integral with variable kernel, the Marcinkiewicz integral with rough variable kernel associated with surfaces of the form...

Ngày tải lên: 22/06/2014, 18:20

... Page of 11 In spite of these benefits, the use of laser-ablationbased in situ conjugation for the generation of aptamerconjugated AuNPs has not yet been reported We show the functionalization of ... http://www.jnanobiotechnology.com/content/8/1/21 Page of 11 Figure Verification of the activity of aptamer-conjugated AuNPs (A) The shift of the SPR maximum clearly indicates the formation of agglomerates in the presence of streptavidin ... conjugated with aptamers against streptavidin TEM micrographs of AuNPs without streptavidin (i), and after incubation with streptavidin (ii) The insert displays a scheme of the proposed composition of...

Ngày tải lên: 11/08/2014, 00:22

... set of all orbifold morphisms f : D → (X, ∆) is relatively compact in Hol(D, X), the set of all holomorphic of D into X The proof of Theorems 3.1 The proof of Theorem 10 PHAM DUC THOAN Proof ... on the set of hyperplanes {Hj }qj=1 , but not the surfaces, such that inequality of type (1.2) holds A natural question is that how the type of the above theorem is in the case of set of hyperplanes ... Weierstrass data We know that the zeros of φdz of order k coincide exactly with the poles of g1 or g2 of 12 PHAM DUC THOAN order k Now the Gauss curvature K of M is given by K=− 2 |φ| (1 + |g1 |2...

Ngày tải lên: 12/10/2015, 16:28

... result of Yau in [21] to see ew Ωds2 = +∞, M because of the minimality of M with respect to the metric ds2 This contradicts the assertion (4.2) The proof of Theorem is completed The proof of Theorem ... distribution of the Gauss maps of complete minimal surfaces in Rm , J Math Soc Japan, 35 (1983),no 4, 663-681 [7] H Fujimoto, On the number of exceptional values of the Gauss maps of minimal surfaces, ... Gauss maps of complete minimal surfaces with finite total curvature These are the strict improvements of all previous results of Fujimoto on the modified defect relations for the Gauss maps of complete...

Ngày tải lên: 14/10/2015, 08:16

... internal organ involvement with onset of symptoms within to weeks of exposure The frequency of the syndrome is estimated at 10–100 per 100,000 persons exposed Risk of serious hypersensitivity ... will perform a selective review of the psychotropic medications often prescribed to patients with bipolar disorder, with an emphasis on their use in patients with medical comorbidity Currently, ... review of effective pharmacotherapies is beyond the scope of this paper which will perform a selective review the psychotropic medications often prescribed to patients with bipolar disorder, with...

Ngày tải lên: 25/10/2012, 10:51

... Ligation of the Pentamer II with the tetrazine-dicarboxilate 6: The DARinv of the pentenoyl-pentamer II with tetrazine-3,6-dimethylcarboxilate µmol (1.86 mg) of the pentamer and 10 µmol (2mg ) of the ... were spatially separated with an amide-based monomer functionalized with a cyclopentane group Further examples of functionalizations Ligation of the pentenoic acid pentamer with the dimethyl-1,2,4,5-tetrazine-3,6-dicarboxylate ... 12 Ligation of Pentamer II with the dansyl-tetrazine Two µmoles (1.86 mg) of the pentamer II (Figure 4) and 10 mmol (8.8 mg) of the dansyl-tetrazine were dissolved in 0.5 ml chloroform/DMSO and...

Ngày tải lên: 25/10/2012, 11:00

... association of disease stage with the rates of CR and severe acute toxicities were analyzed with Fisher’s exact test Circadian variations of plasma concentrations of 5-FU were analyzed with the ... [11-13] A total of measurements of the plasma concentration of 5-FU were made per patient, and it was concluded that the average value was predictive of clinical response, but not of severe acute ... replacement of CDDP with NDP might be of value for a certain subpopulation of patients Although little information is yet available, it was recently reported that NDP was comparable to CDDP with regards...

Ngày tải lên: 26/10/2012, 09:53

... a profound effect on the strategies learners choose, with negative attitudes and beliefs often causing poor strategy use or lack of orchestration of strategies  Type of task: The nature of the ... new beginners to those with the highest levels of proficiency Such a study would help us to get a full idea of the impact of CS in learning vocabulary - 43 - The results of the questionnaire used ... the meanings of the parts to understand the meaning of the whole expression • Analyzing contrastively: Students compare elements (sounds, vocabulary, etc) of English words with those of Vietnamese...

Ngày tải lên: 07/11/2012, 14:54

... acceptance of students with special needs Since one of the principal goals of inclusion for children with special needs is to develop social acceptance and increase positive social interactions with ... inclusion of students with special needs is to ensure that the classroom possesses essential elements of an effective inclusive program with a culture of acceptance Educators have agreed that some of ... to implement Through a combination of teacher interviews, interviews with students with special needs, and classroom observations, the findings of this study offers insight into the strategies...

Ngày tải lên: 06/04/2013, 11:26

Ngày tải lên: 07/06/2013, 01:26

... reaction rate of H2O2 with hydroxyl radical is relatively slow (k= 2.7x107 M-1s-1, Buxton et al (1988)) The relative difference of the respective rate constant of reaction make a decision of OH radical ... different effect of tert-butanol on hydrogen peroxide decomposition and dye removal The presence of tert-butanol caused only slight change of hydrogen peroxide decomposition, whereas 10 mM of tert-butanol ... represented with the expressions of ln ([H2O2]/[H2O2]0) as a function of the reaction time Good linear plot in Figure indicates that the H2O2 decomposition is the first order reaction with H2O2...

Ngày tải lên: 05/09/2013, 08:40

... water with a total volume of 1000mL This stock solution has an arsenic concentration of 1000mg/L - 44 - Journal of Water and Environment Technology, Vol 6, No.1, 2008 Table Composition of synthetic ... series of 1000mL flasks Each of them contained 5g of adsorbents (adsorbent dose) and - 45 - Journal of Water and Environment Technology, Vol 6, No.1, 2008 1000mL synthetic groundwater with various ... velocity of 0.98-1.47m3/m3h), pH of 7-8, and initial arsenic concentration of 0.1-0.5mg/L, to obtain a breakthrough time of more than 100 hours The operation of the column is very sensitive with...

Ngày tải lên: 05/09/2013, 09:38

... assigned with a relatively equal proportion of good, average and poor English proficiency students and these classes are at different levels of English From observation and interviews with teachers of ... student can be well catered for An area of research which has received a lot of attention in many areas of education is the issue of learning style The idea of learning style comes from general ... congress of the Communist Party of Viet Nam, the policy of DOI MOI (Renovation) was adopted New approaches to planning in all fields emerged, affecting all sectors of society Education is just one of...

Ngày tải lên: 07/09/2013, 13:48

... Risk With more than 30 new compliance regulations worldwide, the amount of data that is subject to regulation is growing at 64% per year More than 60% of IT executives now rate compliance with ... levels of efficiency for storing, archiving and managing information with IBM’s expanded suite of ProtecTIER data de-duplication appliances, XIV Storage Systems and Tivoli Storage Management software ... Capacity Rollout Avalanche of data? Need on-demand capacity? Time consuming backups? Store up to 25 TB of data on 1TB of disk storage…FAST! Deploy storage that scales performance with capacity Now there’s...

Ngày tải lên: 16/10/2013, 21:15

... publication is the result of many hours of work by members of the Steel Deck Institute It also greatly benefited from the generous contributions of time, skill and knowledge of many other organizations ... sheet steel into a repeating pattern of parallel ribs The strength and stiffness of the panels are a result of the shape of the ribs and the material properties of the steel Deck lengths can be varied ... stress of 20 ksi with loading of concrete + deck + 20 psf, or concrete + deck + 150 lb concentrated load, whichever is worse 2) Allowable deflection of free edge (based on fixed end cantilever) of...

Ngày tải lên: 12/12/2013, 20:15

... cellular calls without a lot of reconfiguration of the network,” says Adams “It’s just a matter of adding new electronics to the existing Unison system to support those networks.” Case Study With Unison ... coverage with Unison systems are the Bryan Center (an expanded student union housing theaters, restaurants, a coffeehouse, stores, and a post office), and a new extension (opened in 2005) of the ... manager of the finance and administration group at Duke Through his own research as well as consultations with major cellular service providers in the area, Risley came up with a short list of requirements:...

Ngày tải lên: 21/12/2013, 07:17

... Status of CSHCN The population of CSHCN includes children with a wide range of conditions with varying levels of impact and requiring a variety of services This section describes the types of special ... families of only 6.2 percent of children in poverty paid more than $1,000 out of pocket for their children’s care, compared to the families of 29 percent of CSHCN with incomes of 400 percent of poverty ... percentage of CSHCN who currently need or use prescription drugs ranges from 72 percent of CSHCN with family incomes below the poverty level to 83 percent of CSHCN with family incomes of 400 percent of...

Ngày tải lên: 12/02/2014, 11:20

... under-nutrition4 with a prevalence of wasting of 11%.5 Stunting was reported in 44% of Ghanaian children and adolescents and almost all those with SS were underweight, irrespective of height.6 Although ... girls with delay in the age at thelarche and menarche Menarche had not occurred by 14–18 years in 71% of these cases compared with 10% of controls.51 In a study from Gabon, 27% of 131 children with ... 44% of SS cases and, compared with SS cases with normal plasma zinc, was associated with impairment of height, weight, FFM, skeletal growth and sexual and skeletal maturation.125 Supplements of...

Ngày tải lên: 12/02/2014, 19:20