functionalities of tea phenolics in cell cultures

... 0.5 μg/mL epinephrine, 6.5 ng/mL triiodothyronine, μg/mL insulin, ng/mL retinoic acid, 10 μg/mL transferrin, and 0.001% gentamycin (v/v) 16HBE14o- cells were maintained in Dubelco's Minimum Essential ... of small airway epithelial cells Lineage Lineage verification of small airway epithelial cells A & B; Gene expression of CCSP (CC-10) in small airway (SAEC) cell cultures, large airway cell cultures ... Figure Morphology of epithelial cells is maintained over passage Morphology of epithelial cells is maintained over passage Phase contrast micrographs showing no morphological variation in bronchial...

Ngày tải lên: 12/08/2014, 14:20

... grouped into trafficking related clusters such as integrins, Mmps, chemokine and chemokine receptors, and interleukins and interleukin receptors Integrins are key mediators of cell- cell interactions, ... Il18r1 interleukin alpha [NM_010554] interleukin [NM_031168] interleukin 18 [NM_008360] interleukin 17D [NM_145837] interleukin beta [NM_008361] interleukin 11 receptor, alpha chain [NM_010549] interleukin ... paid to genes involved in cell trafficking, namely integrins, metallopeptidases, chemokines and chemokine receptors, as well as interleukins and interleukin receptors (Table 1) In order to visualize...

Ngày tải lên: 12/08/2014, 14:20

... assumptions behind our model of oligodendrocyte development in cell culture In what follows, we describe our experimental findings in the context of the model presented in Section Each element of the ... mammalian cells [28] In that case, the incomplete separation seems to be a consequence of failure of chromatin segregation As our cells are cultured in serum-free defined medium in the absence of any ... O-2A/OPCs increases in the presence of thyroid hormone The probability of differentiation increases from 0.07 in Generation to 0.21 in Generation in the absence of thyroid hormone and from 0.18 in Generation...

Ngày tải lên: 13/08/2014, 23:20

... Gesturing to end the conversation is considered a rude insult 2.2.12 Pointing finger Pointing fingers normally mean indicating some person or some object Extending the index finger to point something ... in most American and European cultures meaning things are going according to your plans or something you approve of However, the going good sign translates into a rude and offensive gesture in ... way to indicate failure 25 2.2.4 Crossing finger Crossing of fingers is considered as a sign of wishing for good luck or fortune It also may interpret that someone is hoping for something good...

Ngày tải lên: 08/11/2014, 23:26

... optimal size remains at Ω≈103 μm3 for a wide range of signal amplitudes and control parameters, which is of the same order of real living cells in vivo Since the internal noise in living cell systems ... that, near the Hopf bifurcation point, instead of trying to resist the internal molecular noise, living cell systems may have learned to exploit the internal noise to intensively enhance the ability ... may often receive weak signals, our findings may have quite significant implications for living cell systems and may imply the ubiquitous importance of internal noise in functioning processes in...

Ngày tải lên: 02/09/2015, 13:20

... significantly modified the quantity of identified proteins involved in protein folding and metabolism out of a total of 26 identified proteins (Baik et al 2006) Six proteins including VIM, GAPDH, LGALS1, ... 2.2.4.4 Interaction of IF4E with 4E binding protein Another widely accepted translational control mechanism involves inhibition of the binding between IF4E and IF4G during the formation of the ... activity thereby extending cellular growth xi by days and increasing final recombinant protein titer by 4-fold The mTOR pathway appeared thus to influence the fate of CHO cell cultures via translational...

Ngày tải lên: 08/09/2015, 19:49

... area of great interest, as loss of genomic integrity is often implicated in tumorigenic and oncogeneic events Critical to the ability of healthy cells in maintaining genomic integrity are the cell ... Induction in Hela cells in response to Adriamycin and varying doses of p38 inhibitor Figure 5.7: Inhibition of p38 induces apoptosis in A549 cells Figure 5.8: Apoptosis induction by MMS and LY479754 in ... associated proteins including Nlp (ninein-like protein), Katanin and the microtubule stabilizing protein TCTP (47,296) Besides being involved in the active recruitment of key components of the microtubule...

Ngày tải lên: 11/09/2015, 09:02

... chromatin resulting in either gene activation or repression For instance, the appearance of histone acetyltransferases, including cAMP-responsive element binding (CREB) protein-binding protein (CBP), ... 2000) CDK protein levels remain stable during the cell cycle, in sharp contrast to their activating proteins, the cyclins The oscillatory expression of most cyclin proteins during cell cycle progression ... the cyclin-dependent kinases (CDKs), a family of serine/threonine protein kinases that are activated at specific points of the cell cycle To date, nine CDKs have been identified and, of these,...

Ngày tải lên: 13/09/2015, 20:18

... assays following the method of Bradford (1976) using bovine serum albumin as a standard The final protein content of the intracellular fraction in 100 ml of liquid medium was mg Preparation of mycelial ... spruce contains more isorhapontin than astringin (Fig 3) The rapid decline of the levels of glycosides was described in in vitro maintained excised bark discs of Sitka spruce following the fungal ... decrease in concentrations of stilbene glycosides The decrease in isorhapontin (occurring in the highest concentrations in embryogenic cell cultures) , astringin and piceid levels was observed in WF-elicited...

Ngày tải lên: 07/08/2014, 10:21

... precursor cells [34] but may also appear in cultured epithelial cells [35] To distinguish between different populations of cells, double labeling of cellular cytokeratin and vimentin was performed Cells ... number of cells only in P1-F344-cells, but not in P2-F344- and P2-Lewis-cells Proportion of SA-b-gal-positive cells did not correlate with cell number, as this amount of cells was not altered in ... showing distinct sensitivity Thus, the impact of a-amylase on cell growth in vitro depends on cellular conditions, origin, e.g rat strain, and distinct cellular characteristics The rat primary cells...

Ngày tải lên: 10/08/2014, 10:21

... Live/Dead staining, a method involving rinsing of cell cultures, mainly measured firmly attached cell attachment and viability These results emphasize the importance of consideration of the type of assay ... effect of Cd2+ ion on cell detachment is more prominent than that on cell viability Determination of effect of different media and acid treatment on size and integrity of in- house synthesized QDs In ... toxicity of QDs in Caco-2 cell cultures, treated QDs were diluted to working concentrations in cell culture medium (pH 7.4) and incubated with Caco-2 cells cultured in a 96-well plate for 24 hours in...

Ngày tải lên: 11/08/2014, 00:22

... and maintaining all transduced cell lines AK and RD were instrumental in providing support to the research in the form of both instruction in and preparation of all lentivirus vectors used in this ... transduced cells were maintained in complete L-15 with 35 μg/mL hygromycin (Invitrogen) For infection of cells, complete L-15 without any antibiotics was used Cells were incubated at 28°C without CO2 Infection ... hRz expressing C6/36 cells using qRT-PCR to detect virus genomes in cell lysates Total cellular RNA was extracted dpi from infected hRz-transduced cells following the protocol described in Materials...

Ngày tải lên: 12/08/2014, 04:21

... gradient in Arabidopsis pistils was implicated in the misguidance of pollen tubes suggesting a role for GABA in intercellular signaling [43] β-Alanine is a non-protein amino acid synthesized mainly ... increased in abundance later than most amino acids The concentration of asparagine also increased in Pseudomonas syringae-infected tomato leaves, suggesting that asparagine, and not glutamine is ... tobacco cell cultures, PLA2 was also induced in response to elicitor treatment [29] In opium poppy cells, only Ophosphocholine increased in response to elicitor treatment The first step in choline...

Ngày tải lên: 12/08/2014, 05:20

... proteins indeed changes the expression of apoptosis profile of host cells, which probably results in the carcinogenesis of liver cells Materials and methods Cell lines and isolation of RNA HepG2 cell ... important regulators of cell apoptosis, it is possible that HBV infection might alter the cIAPs gene expressing of liver cell, finally instigating the carcinogenesis of the cells it infected This is ... of cIAP2 was obviously higher in the HepG2.215, a cell line expressing HBV, than in the HepG2, a cell line absence of HBV (Figure 1A, 1&2) The up-regulation of cIAP2 expression in HepG2.215 cells...

Ngày tải lên: 02/11/2012, 11:17

... reforming or partialoxidation reforming In the INER SOFC system, the reforming process is performed using a combination of these two methods by feeding both steam and an oxidant (air) into a ... efficiency of more than 40% In the beginning, according to the single cell P-I-V curve to find the long term operation of voltage, current, active area and cell number Figure Flow chart showing GCTool ... as the input fuel Porous media afterburners provide an efficient means of ensuring the complete conversion of the SOFC off-gases during nominal operation and of pre-heating the cathode intake...

Ngày tải lên: 05/09/2013, 16:10

... expression of an E2F variant containing the DNA binding motif but not the pRb-binding or transactivation domain or introduction of a competitor plasmid containing multiple E2F binding sites, preventing ... for binding to proteins containing LXCXE motifs while the pocket-domain and the C-domain are involved in binding E2F proteins Mdm2 (as well as c-Abl) binds to the C-domain All pocket proteins ... encoding a pRBbinding protein with properties of the transcription factor E2F Cell 70:337–350 Helin K, Harlow E, Fattaey A (1993) Inhibition of E2F-1 transactivation by direct binding of the retinoblastoma...

Ngày tải lên: 25/10/2013, 21:20

... may be ‘trapped’ in chronicity by an incapacitating sick role The lingering effects of ‘institutionalism’ among discharged patients of that era offer indirect corroboration (Wing and Brown, 1970) ... of evidence inconveniently lying elsewhere The rumour of recovery from schizophrenia in other cultures made innocent rounds in early ethnographic circles only to be roundly discredited in clinical ... odds of recovery ratio of roughly 1.5 favouring the non-industrial group During this period, too, independent reports from other ‘developing’ regions continued to appear, employing a host of methods...

Ngày tải lên: 01/11/2013, 09:20

... of ginseng cell and some attempts have been made to increase biomass and ginsenoside yield of Ngoc Linh ginseng cell culture Materials and methods Induction of callus Fresh mountain ginseng roots ... Suspended cells of P vietnamensis were initiated through callus induction from the cultivated plant root [5] The cell line was maintained in MS liquid medium supplemented with mg/L indole-3-butyric ... suspension cultures of P vietnamensis for biomass and production of ginseng saponin (secondary metabolite) The final dry cell weight was increased from 5.4 to 10.3 g/L with an increase of initial sucrose...

Ngày tải lên: 12/02/2014, 10:20

... intracellular calcium level increased in OccWT-overexpressing cells, and that 2-APB inhibited the effects of OccWT Therefore, the increase in intracellular calcium may explain the induction of ... function of occludin, we reduced occludin expression in Huh7 cells by using occludin short hairpin RNA (shRNA) After determining the reduced expression level of occludin with immunoblotting (Fig ... facilitates the initiation of apoptosis [22] In addition, elevated calcium levels contribute to cell invasion [23,24] In contrast, in the case of occludin, an ER-mediated increase in intracellular calcium...

Ngày tải lên: 18/02/2014, 18:20