function of myc for generation of induced pluripotent stem cells

... SCREEN OF NUCLEAR RECEPTORS FOR THE ENHANCED AND ALTERNATIVE GENERATION OF INDUCED PLURIPOTENT STEM CELLS DOMINIC HENG JIAN CHIEN (B.Sc (Hons), NTU) A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF ... the process of pluripotential reprogramming in which the lineage restriction of differentiated cells is undone The resultant cells, known colloquially as induced pluripotent stem cells (iPSCs), ... akin to pluripotent embryonic stem cells (ESCs) and pluripotent cells of the early embryo, thus gaining their characteristic developmental potential and other characteristics diagnostic of pluripotent...

Ngày tải lên: 09/09/2015, 18:56

... Department of Chemical and Systems Biology, Stanford University School of Medicine, Stanford, CA 94305, USA 2Department of Developmental Biology, Stanford University School of Medicine, Stanford, ... embryonic stem cell; hESC, human embryonic stem cell; H3K4me3, trimethylation of lysine of histone H3; H3K27ac, acetylation of lysine 27 of histone H3; H3K27me3, trimethylation of lysine 27 of histone ... embryonic stem cell; H3K4me1, methylation of lysine of histone H3; H3K4me3, trimethylation of lysine of histone H3; H3K27ac, acetylation of lysine 27 of histone H3; H3K27me3, trimethylation of lysine...

Ngày tải lên: 11/08/2014, 12:21

... fibroblasts to Induced Pluripotent Stem Cells 45 iv 4.10 Culture of Porcine Induced Pluripotent Stem Cells 45 4.10 Characterization of Porcine Induced Pluripotent Stem Cells 46 4.10.1 Immunocytochemical ... Pluripotent Stem Cells ……………… 44 4.8 Infection of fibroblast cells to form Induced Pluripotent Stem Cells …………………………………………………………………… 45 4.9 Culture of cells transitioning from fibroblasts to Induced ... differentiation of Human IPS cell lines 27 3.5 Generation of Porcine Induced Pluripotent Stem Cells 29 3.5.1 Factors used for reprogramming of porcine cells 29 3.5.2 Reprogramming techniques of porcine...

Ngày tải lên: 30/09/2015, 10:13

... genetic approach for the generation of induced pluripotent stem cells Cell Stem Cell 2008, 2:525-528 Shi Y, Desponts C, Do JT, Hahm HS, Schöler HR, Ding S: Induction of pluripotent stem cells from ... JA: Induced pluripotent stem cell lines derived from human somatic cells Science 2007, 318:1917-1920 Okita K, Nakagawa M, Hyenjong H, Ichisaka T, Yamanaka S: Generation of mouse induced pluripotent ... Takahashi K, Ichisaka T, Aoi T, Okita K, Mochiduki Y, Takizawa N, Yamanaka S: Generation of induced pluripotent stem cells without Myc from mouse and human fibroblasts Nat Biotechnol 2008, 26:101-106...

Ngày tải lên: 14/08/2014, 21:20

... expansion of human embryonic stem cells, and more recently, of human induced pluripotent stem cells The exploration and realization of the incredible potential of these pluripotent stem cells for studying ... reprogramming of adult skin fibroblasts into induced pluripotent stem cells (Takahashi and Yamanaka, 2006) To realize the full potential of embryonic and induced pluripotent stem cells, technologies, ... principles of karyotyping and fluorescent in situ hybridization (FISH) methods as they apply to the field of pluripotent stem cells Induced pluripotent stem cells attract great interest for their...

Ngày tải lên: 22/03/2014, 09:20

... capable of generating spontaneous synaptic activity [45] These two studies exemplify how pluripotent stem cells can differentiate into functional neurons of subtypes relevant for the study of AD ... monogenic and complex diseases with pluripotent stem cells Nat Rev Genet 2011, 12:266-275 33 Gonzalez F, Boue S, Belmonte JCI: Methods for making induced pluripotent stem cells: reprogramming a la carte ... signatures for the isolation of neural stem cells, glia and neurons derived from human pluripotent stem cells PLoS One 2011, 6:e17540 48 Funato H, Yoshimura M, Yamazaki T, Saido TC, Ito Y, Yokofujita...

Ngày tải lên: 11/08/2014, 12:21

... differentiation of cultured human mesenchymal stem cells from marrow Tissue Eng 1998, 4:415-428 35 Mueller MB, Tuan RS: Functional characterization of hypertrophy in chondrogenesis of human mesenchymal stem ... the differentiated function of osteoblasts, which have led to the clinical application of these proteins for bone regeneration [1,7-10] We and others have tested several BMPs for their potential ... for apoptosis detection through binding to phosphatidylserine epitopes on the plasma membrane of early Page of 15 (page number not for citation purposes) apoptotic cells, and the hydrolysis of...

Ngày tải lên: 09/08/2014, 14:22

... application of induced pluripotent stem cell technology for therapeutic purposes Patient-derived somatic cells can be isolated through tissue biopsies and converted into induced pluripotent stem cells ... programs of human embryonic and induced pluripotent stem cells Cell Stem Cell 2010, 7:249-257 Wilson KD, Venkatasubrahmanyam S, Jia F, Sun N, Butte AJ, Wu JC: MicroRNA profiling of human -induced pluripotent ... dysregulation of gene expression [29] ESC, embryonic stem cell; hiPSC, human induced pluripotent stem cell; iPSC, induced pluripotent stem cell; miRNA, microRNA complex to control neural crest formation...

Ngày tải lên: 11/08/2014, 12:21

... of 11 Mesenchymal stem cell treatment has no effect on the development of collagen -induced arthritis Figure Mesenchymal stem cells (MSCs) inhibit the anti-CD 3induced proliferation of CD4+ T cells ... Page of 11 Figure Mesenchymal stem cells (MSCs) inhibit the proliferation of CD4 + T cells in vitro by induction of nitric oxide and prostaglandin E2 (PGE2) (a) CD4+ T cells, in the presence of ... CD4+ T cells (5 × 104 cells) and accessory cells (5 × 104 cells) were incubated with μg/ml anti-CD3 antibody and the indicated numbers of mitomycin c-treated wild-type or IFN-gR KO MSCs for 72...

Ngày tải lên: 12/08/2014, 11:23

... NANOFIBERS FOR OSTEOGENIC DIFFERENTIATION OF HUMAN MESENCHYMAL STEM CELLS NGUYEN THI HIEN LUONG (B.Eng., Ho Chi Minh city University of Technology, Vietnam) A THESIS SUBMITTED FOR THE DEGREE OF ... Casey K Chan Recovery of Osteogenic Ability of Human Mesenchymal Stem Cells during Serial Passage Using Nanofibrous Scaffolds Proceedings of International Conference on Materials for Advanced Technologies ... 43 2.3 Stem cells 51 2.3.1 Definition and classification 51 2.3.2 Stem cells in tissue engineering 53 2.3.3 Mesenchymal stem cells 54 v 2.4 Stem cell...

Ngày tải lên: 09/09/2015, 10:07

... Institute (BTI) and the Agency for Science Technology and Research (A*STAR) Thanks to Prof Wang Nai-Dy, Prof Miranda Yap, Prof Tan Bor Leung, Prof James Chen, and Dr Paul Robson for their guidance, in ... ECOPak cells Figure 15 Antibiotic selection of mESC – kill curve analysis Figure 16 Generation of Tet-R mESC lines, establishment of transient shRNA vector knockdown Figure 17 Morphology of stably ... (solid line) (B) Immunofluorescence of AP marker for pluripotent mESC showed the predicted expression in the cultures, as well as (C) SSEA-1, a surface antigen specific for pluripotent mESC Both...

Ngày tải lên: 27/11/2015, 11:25

... mitotic spindle is unable to form Hence, for reference to the initial number of cells before exposure to low temperature, a 6-well culture dish with the same 125 density of hESC colonies was subjected ... 4oC for 24h 1.51 ± 0.10 64.4 ± 4.4 % 25oC for 24h 1.81 ± 0.11 77.3 ± 4.8 % 4oC for 48h 1.62 ± 0.06 69.0 ± 2.3 % 25oC for 48h 1.66 ± 0.01 71.0 ± 0.5 % Assessment of spontaneous differentiation of ... with weak expression of SSEA3 and TRA-1-81 A total of 200 colonies were examined for each experimental group, as well as for the control Statistical comparison of data was performed by the Chi-squared...

Ngày tải lên: 31/10/2012, 16:57

... cases except that of the Rex-1 gene, primer sets span introns Day cells are composed of ES cells and MEF cells grown in ES media containing leukemia inhibitory factor (LIF) These cells express the ... primer that hybridizes just downstream of The commitment of pluripotent stem cells to successively less plastic progenitors and, finally, differentiated cells exhibiting stable expression patterns ... the differentiation of hematopoietic stem cells to erythroid cells Transcription of HS3 could be important in maintaining this accessible structure, particularly in light of the fact that RNA...

Ngày tải lên: 07/03/2014, 12:20

... 13 The expression of miR-92 has been reported in human embryonic stem (ES) cells [16,26], mouse ES cells[ 20] or Page 12 of 17 (page number not for citation purposes) Journal of Translational Medicine ... for comparison It should not be forgotten that hES cells contain spontaneously differentiated cells, so it is difficult to precisely determine which type of cells express miR-92 The members of ... undifferentiated and differentiated human embryonic stem cells Stem Cells Dev 2007, 16(6):1003-1016 Page 15 of 17 (page number not for citation purposes) Journal of Translational Medicine 2009, 7:20 18 19...

Ngày tải lên: 18/06/2014, 15:20

... such as CD34 and CD133 allowing for the isolation of relatively pure populations of these cells for in vitro study of the cellular processes in thalassemic stem cells While previous attempts have ... CD 34+ cells were then flushed from the column The isolated HSCs/CD34+ cells were harvested for phosphoproteomic analysis and cultivation of erythroid cells The cultivation of erythroid cells ... method of isolation was performed as described in the manufacturer’s protocol 1-2 × 108 cells of MNCs were reacted with 100 μl of anti-CD 34 antibody coated with micro beads and 100 μl of anti-FcR...

Ngày tải lên: 18/06/2014, 19:20

... trabecular bone area); fat cells diameter = the total diameter of fat cells in selected field/the number of fat cells in selected field; fat cells area = the area of all fat cells in selected field/(the ... marrow lipid was a consequence of the adipogenesis of marrow mesenchymal stem cells (MSCs) [7] Results of a previous study showed increased number of small size fat cells in the early steroid-associated ... the density of Oil Red Page of (page number not for citation purposes) Journal of Orthopaedic Surgery and Research 2007, 2:15 O positive cells were calculated using Image Pro Plus software (Media...

Ngày tải lên: 20/06/2014, 00:20

... such as CD34 and CD133 allowing for the isolation of relatively pure populations of these cells for in vitro study of the cellular processes in thalassemic stem cells While previous attempts have ... CD 34+ cells were then flushed from the column The isolated HSCs/CD34+ cells were harvested for phosphoproteomic analysis and cultivation of erythroid cells The cultivation of erythroid cells ... method of isolation was performed as described in the manufacturer’s protocol 1-2 × 108 cells of MNCs were reacted with 100 μl of anti-CD 34 antibody coated with micro beads and 100 μl of anti-FcR...

Ngày tải lên: 20/06/2014, 04:20

... from residual stem cells The existence of stem cells in the adult brain suggests that it has the potential for self-repair and that newborn neuronal cells may contribute to the functioning, and ... stimulation of endogenous neural progenitor or stem cells and the transplantation of adult-derived neural progenitor and stem cells [39] Self-renewing multipotent neural progenitor and stem cells have ... progenitor or stem cells locally would represent a strategy to promote regeneration of the diseased and injured nervous system Alternatively, new neuronal cells are generated at sites of degeneration...

Ngày tải lên: 08/08/2014, 17:20

... Examples of canonical Wnt functions include the promotion of long-term culture expansion of stem cells, increased in vivo reconstitution of hematopoietic lineages, and Wnt3a-specific maintenance of ... the induction of MSC adipogenesis Myogenesis Most investigations of myogenesis in adult stem cells are based on a small population of skeletal muscle-derived stem cells, or satellite cells A recent ... is the homing of stem cells to sites of injury and subsequent wound healing Although some tissue repair may be accomplished by the division of indigenous differentiated cells, such cells are most...

Ngày tải lên: 09/08/2014, 10:20

... 10% (vol/vol) formaldehyde (Sigma-Aldrich) at room temperature for 15 minutes The monolayers were then washed twice with excess distilled water before addition of 250 μl of 40 mmol/l of Alizarin ... mesenchymal transcripts Expression of BMP-2 and chordinstem cells during osteogenic differentiation of human mesenchymal stem cells (a) Temporal progression of bone morphogenetic protein (BMP)-2 and chordin ... suppression of chordin led to an increase in the bioavailability of endogenously produced BMP-2 to drive the differentiation of Page of (page number not for citation purposes) Effect of chordin...

Ngày tải lên: 09/08/2014, 10:23