francisco h peter plant proteome analysis proteomics 4 2004 285 298

... Expression proteomics relies on a mix of, on the one hand, high-tech approaches and on the other, a harvest of know-how in protein chemistry and biochemistry gained over the past half-century Although ... 60 high-throughput 72, 97, 223 HMW proteins 186, 187 homo-arginine 1 34 homogeneous 2 04 gel 60 polyacrylamide gels 69 horizontal streaking 40 hot Coomassie Blue solution 228, 2 34 HPLC 118 hybrid ... manufacturing practice Hour a-cyano -4- hydroxycinnamic acid Helium neon N-2-hydroxyethylpiperazine-N¢-2-ethananesulfonic acid High Molecular Weight High Performance Liquid Chromatography Current in A,...

Ngày tải lên: 08/04/2014, 13:08

... exchanger Mixed mode Aminopropyl (NH2) group Cyanopropyl (CN) group Diol (OH) group Characteristic Reversed phase: highly hydrophobic Reversed phase: highly hydrophobic Reversed phase: hydrophobic ... inserted through the septum to draw the headspace gas containing a target compound This method is very suitable for gas chromatographic analysis The headspace method is widely used for analysis ... Analysis in Hearth Science Kanehara-shuppan, Tokyo, pp 372–382 7) Tsuchihashi H, Nakajima K, Nishikawa M et al (1991) Determination of methamphetamine and amphetamine in urine by headspace gas chromatography/mass...

Ngày tải lên: 22/01/2014, 00:20

... discoidum [ 34, 35] A common theme of these studies is the requirement of a comprehensive proteome analysis in order to acquire an image of the organelle investigated This highlights the unique potential ... Biochem 199, 223–231 Werhahn, W & Braun, H. P (2002) Biochemical dissection of the mitochondrial proteome from Arabidopsis thaliana by three-dimensional gel electrophoresis Electrophoresis 23, 640 – 646 ... reinhardtii Plant J 28, 595–606 42 Gerner, C., Frohwein, U., Gotzmann, J., Bayer, E., Gelbmann, D., Bursch, W & Schulte-Hermann, R (2000) The Fas-induced apoptosis analyzed by high throughput proteome analysis...

Ngày tải lên: 23/03/2014, 20:22

... Q9JIL3 Q5BJY9 P 845 88 P63018 P057 84 7513586a P681 34, P60710 P63259 Q5PQX1 P 343 05 Q9Z0W3 P70168 42 36 34 75 23 36, 48 74 65 74, 65 47 , 36 65 175 66 98 35 77 54 54 54 98 47 S M M S M M S S S M M M S ... A: 10 mm Tris ⁄ HCl, pH 7 .4, 0.2 mm MgSO4; m urea buffer: 10 mm Tris, m urea, 0.1 m NaH2PO4, pH 8.0; NaCl ⁄ Pi: 1.5 mm NaH2PO4, 8.1 mm Na2HPO4, 145 mm NaCl Animals Animal care, housing and killing ... After incubation with 0.1 m monoethanolamine, pH 8.0, m urea at room temperature for h, the affinity resin bearing His6ISP36 was thoroughly washed with 20 mm Tris ⁄ HCl, pH 8.0, 0.1% (w ⁄ v) SDS,...

Ngày tải lên: 30/03/2014, 20:20

... crucial steps in proteome analysis DATABASES AND I NFORMATICS The high-throughput methodologies that have been developed for both DNA and proteomics have highlighted the need for sophisticated informatics ... in the chromosome sizes Within the legumes this has been extensively characterized For example, both Vicia faba and Lotus tenuis have a chromosome number of 6, whereas the lengths of these C H ... such as the Alu elements in humans and perhaps the expansion of the 5S ribosomal RNA genes in flax, are demonstrations of this behavior In all the plants in which they have been investigated the...

Ngày tải lên: 08/04/2014, 13:07

... 08 04 04 04 0.8 04 05 04 05 05 40 0.5 -0 05 2.5 05 0.5 1.0 80 05 0.5 40 0.5 12 0.5 1.0 1006 10 01 0.01 0.6 06 0.8 02 04 08 04 0.2 04 0 .4 10.0 04 0.6 0.12 08 02 02 0.05 04 0.2 05 0 .4 04 04 05 ... Nuclear [NEW 32P-orthophosphate (NEN) Phosphate-buffered saline (PBS): Mix g NaCl, 0.2 g KCl, 1 .44 g of Na2HP 04, and 0. 24 g KH,PO, m 800 mL of dH,O, and adjust pH to with HCl Add dH20 to a final volume ... generally much higher than the one of the sample This process is, however, rather difficult for tube gels m carrier ampholytebased IEF The mam difficulty artses from the fact that the thtol compounds...

Ngày tải lên: 10/04/2014, 10:57

... thống kê thanh, bảng ồm thống kê thép (chưa có kết quả) Có thể tiến h nh tính toán cách chọn tham biến h p thoại tính toán h nh Nếu muốn thay đổi thông số tính toán, nghiên cứu thông số tính ... diên tích cốt thép tính cho phần tử h u h n 0, cố thép tối thiểu không khởi tạo (h nh bên trái)  For the whole panel: cốt thép tốt thiểu khởi tạo cho toàn (h nh bên phải) Nguyễn Văn Thiệp Autodesk ... Structural Analysis – Tính toán cốt thép cho cấu kiện H p thoại Tại có hai qui phạm mặc định cho cột dầm Nhấn đúp vào qui phạm, h p thoại Trong h p thoại chứa nội dung qui phạm chọn IV.2.1.1.1 Định nghĩa...

Ngày tải lên: 08/06/2014, 07:46

... Spatial Analysis   103 Data Mapped with Four Different Classification Methods (6 Classes) 64 736 1 041 40 43 >40 43 43 68 >43 68 48 14 >48 14 6168 6950 64 1212 >1212–2359 >2359–3507 >3507 46 55 >46 55–5802 ... a hypothesis that had to be further studied or tested He thus limited the use of the information from his analysis and had a sound basis for choosing his data sources and how he would use this ... wealthy areas? With the flood depth grid shown in Figure 4. 4 for the City of New Orleans during Hurricane Katrina, one could use spatial analysis to determine if a higher percentage of households...

Ngày tải lên: 18/06/2014, 22:20

... and the references there in) Other forms of shear deformation theory, such as the third order-shear deformation theory (TSDT) that accounts for the transverse effects, have been considered Cheng ... different thickness to length ratios It can be seen that nonlocal theories predict smaller values of natural frequencies than local theories especially for higher thickness to length ratios Thus the ... ceramic plate, respectively The thickness coordinate variable is presented by x3 while  h h  x3  , where h is the total thickness of the plate as shown in Figure p  is 2 the volume fraction exponent...

Ngày tải lên: 19/06/2014, 19:20

... general, the physical and chemical properties and the performance of the ethyl esters are comparable to those of the methyl esters The methyl and ethyl esters have almost the same heat content The viscosities ... temperature The viscosities of the methyl esters from the vegetable oils were slightly higher than that of No diesel fuel Figure 4. 5 shows a typical example of the relationship between the reaction ... make methyl esters of the straight chain fatty acid Alcohols are primary or secondary monohydric aliphatic alcohols having one to eight carbon atoms Among the alcohols that can be used in the transesterification...

Ngày tải lên: 20/06/2014, 00:20

... event allows the opening of the valve when the channel gets equal (ch=1); then the transition “[ch]/v1=1” is run; and after the sensor detects the total advance of the cylinderarm, the valve must ... trigger other transitions automatically in the model This characteristic decreases the number of states produced in the reachability tree • The part of the algorithm that describes the history ... an inherent characteristic of original Statecharts, is accomplished through ANDdecomposition However, the component synchronism demands additional mechanisms Consider a plant composed of three...

Ngày tải lên: 21/06/2014, 14:20

... The further out the time horizon, the less reliable the forecast That’s why I have limited VantagePoint’s fore- Trend Forecasting With Technical Analysis 67 casts to four trading days, which ... accounts of the various means that technicians have devised to reduce the lag effect While each type of moving average has its own strengths and weaknesses at smoothing the data and reducing the lag, ... with changes in the market’s price A simple moving average lags behind the market because it gives equal weight to each period’s price This limitation is what has prompted the use of weighted...

Ngày tải lên: 04/07/2014, 13:20

... which the author intended to have subordinated I am not sure that he was always right in his emphasis, especially with reference to the biographical sketches, which appeal to most people who have ... December 1 948 — ‘Science and Ideology’—was concerned with some of these problems of methodology which he takes up in Part I of the History The course which he gave at Harvard on the History of ... of the History written in the early years which were not later rewritten are the chapter The ‘Mercantilist’ Literature, which was typed in June 1 943 , the chapter on Sozialpolitik and the Historical...

Ngày tải lên: 04/07/2014, 18:20

... rubbery, is where chains gain enough mobility to slide by each other Below the Tg, the behavior of the polymer is dominated by the free volume, vf, which limits the ability of the chains to move ... the complex viscosity usually agrees fairly well with the steady shear viscosity One applies the Gleissele’s mirror relationship, which is h( dg dt ) = h+ (t ) t =1 ( dg / dt ) (4. 18) where h+ (t)| ... the phase angle, b is the sample geometry term, fo is the force applied at the peak of the sine wave, and k is the sample displacement at the peak The tangent of the phase angle is one of the...

Ngày tải lên: 05/08/2014, 09:19

... triglycerides were hydrolyzed using hot methanol and KOH, followed by derivatization Chromatographic conditions The HPLC method presented here was used in the analysis of the fatty acid pattern ... hydrolyzed with 500 µl MEOH/ KOH at 80 ºC for 40 in a thermomixer After cooling 1.5 ml ACN/THF (1:1) was added, and the mixture was shaken for The mixture was then filtered through a 0 .45 -µm Minisart ... Adulteration with foreign fats and the use of triglycerides that have been modified by a hardening process also can be detected through triglyceride analysis The HPLC method presented here was used...

Ngày tải lên: 06/08/2014, 13:22

... 45 .69 45 .67 45 .60 45 . 64 45.66 45 .63 45 .71 45 .63 45 . 64 45.71 45 .67 45 .65 45 .69 0.10 –0.06 –0.03 0.11 0.03 –0.01 0.06 0 .42 14. 31 14. 20 14. 30 14. 31 14. 25 14. 31 14. 23 14. 20 14. 32 14. 21 14. 20 14. 26 14. 20 ... 4. 38 4. 42 4. 30 4. 40 4. 34 4.38 4. 39 4. 35 4. 31 4. 43 0.06 –0.06 0.02 0.05 –0.02 –0.12 0.12 0.32 3.96 3. 94 4.00 3. 94 3.96 3.96 3.92 4. 00 4. 02 3.96 4. 02 4. 00 4. 00 4. 04 0.03 0.08 –0. 04 0.07 0.05 0. 04 ... 24. 4 SD 0.06 avg 24. 3 SD 0.03 24. 2 0.15 24. 4 0.08 24. 5 0.02 24. 4 0.07 24. 2 0.13 24. 2 0.13 24. 3 0.08 24. 2 0.03 24. 0 0.10 24. 3 0.02 24. 1 0.06 24. 1 0. 04 24. 2 0.12 24. 3 0.09 24. 3 0.06 = Copyright...

Ngày tải lên: 06/08/2014, 13:22

... in Nasdaq sample 1 741 1 742 The Journal of Finance (c) (d) Figure Continued Foundations of Technical Analysis (e) (f) Figure Continued 1 743 1 744 The Journal of Finance (g) (h) Figure Continued ... Figure Continued Foundations of Technical Analysis (g) (h) Figure Continued 1739 1 740 The Journal of Finance (i) (j) Figure Continued Foundations of Technical Analysis (a) (b) Figure Distribution ... 1736 The Journal of Finance (a) (b) Figure Distribution of patterns in NYSE/AMEX sample Foundations of Technical Analysis (c) (d) Figure Continued 1737 1738 The Journal of Finance...

Ngày tải lên: 07/08/2014, 01:22

Ngày tải lên: 08/08/2014, 03:20