fractionation nuclear protein extraction and western blot analysis for inos i amp kappa b amp alpha nf amp kappa b p65 bax and bcl 2

dien di protein va lai western blot potx

Ngày tải lên : 20/06/2014, 21:20

... ph bin nht Thit b v vt liu in di Mỏy in di B ngun in Dung dch m Mt bn gel Cỏc cht nhum CU TRC V C TNH PROTEIN Cu to t khong 20 loi axit amin Cu trỳc bc I, II, III, IV Chui polypeptid ... Mậu ,20 00) Hình ảnh i n di protein lạc Hình ảnh i n di protein lúa in di da vo im ng in im ng in ca mt protein (pI) l im pH m ú in tớch thc ca nú bng Ti im pH ny, tớnh di ng ca protein in ... tớch in (+) hoc (-) Mi loi protein thi cú kớch thc v s cun xon khỏc Quy trỡnh chung in di protein CHIT PROTEIN BIN TNH PROTEIN IN DI CHụP ảNH Và PHÂN TíCH KếT QUả 1.in di mt chiu SDS-PAGE in...

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$Báo cáo khoa học: Proteome analysis of a rat liver nuclear insoluble protein fraction and localization of a novel protein, ISP36, to compartments in the interchromatin space pptx$

Báo cáo khoa học: Proteome analysis of a rat liver nuclear insoluble protein fraction and localization of a novel protein, ISP36, to compartments in the interchromatin space pptx

Ngày tải lên : 30/03/2014, 20:20

... to ribosome FEBS Journal 27 2 (20 05) 4 327 –4338 ª 20 05 FEBS Interchromatin space protein ISP36 18 19 20 21 22 23 24 25 26 27 28 29 30 binding I Identiﬁcation of ribophorins I and II, membrane proteins ... heterogeneous nuclear ribonucleoprotein G, functions in alternative splicing [26 ] Interleukin enhancer-binding factor binds protein- arginine methyltransferase and contains double-stranded RNA-binding motifs ... Coomassie Brilliant Blue (B1 and B4 ) or transferred to a nitrocellulose sheet (B2 , B3 , B5 and B6 ) followed by immunostaining For the immunostaining, nitrocellulose strips were incubated with 20 0-fold...

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Báo cáo khoa học: Functional analysis of a murine monoclonal antibody against the repetitive region of the fibronectin-binding adhesins fibronectin-binding protein A and fibronectin-binding protein B from Staphylococcus aureus pot

Ngày tải lên : 06/03/2014, 22:21

... We identified six potential highaffinity Fn-binding motifs (FnBPB-1, FnBPB-4, FnBPB-5, FnBPB-9, FnBPB-10, and FnBPB-11) in both proteins, and low-affinity binding sites in the remaining motifs This ... mediate activation of human platelets via fibrinogen and fibronectin bridges to integrin GPIIb ⁄ IIIa and IgG binding to the Fc gammaRIIa receptor Mol Microbiol 59, 21 2 23 0 Casolini F, Visai L, ... possesses fibrinogen-binding [22 ] and tropoelastin-binding abilities, mediated by the N-terminal A-domain region [23 ] The Fn-binding moiety is organized into 11 tandem repeats, each interacting with...

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Báo cáo khoa học: Adenine nucleotides inhibit proliferation of the human lung adenocarcinoma cell line LXF-289 by activation of nuclear factor jB1 and mitogen-activated protein kinase pathways doc

Ngày tải lên : 07/03/2014, 12:20

... progression and proliferation Inhibition of CaMKII activity by KN- 62 (25 lm) completely reduced the inhibition by ATP The inhibition of LXF -28 9 cell proliferation by 100 lm ATP was totally attenuated by ... be involved in ATP-mediated inhibition of LXF -28 9 cell proliferation (Table 2) Inhibition of the MAPK cascade enzymes MEK1 ⁄ and p38 MAPK by their selective inhibitors PD98059 ⁄ U0 126 and SB203580, ... the involvement of NF- jB in nucleotide-mediated inhibition of LXF -28 9 cell proliferation Preincubation of LXF -28 9 cells with the NF- jB1 inhibitory peptide NF- jB SN50 (20 lm) completely abrogated...

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Báo cáo khoa học: Spermosin, a trypsin-like protease from ascidian sperm cDNA cloning, protein structures and functional analysis doc

Ngày tải lên : 17/03/2014, 11:20

... termini and the proline-rich regions in the C termini of mammalian proacrosins In addition, we found that the 28 -kDa vitelline coat protein is capable of binding to the above proline-rich region ... only with the GST-L1 or GST-L1(DL2) fusion proteins, but not with the GST-L2 fusion protein (Fig 4B) , indicating that the 28 -kDa protein of the vitelline coat has an ability to bind to the proline-rich ... trypsin-like protease, distinct from acrosin, a well-known sperm trypsin-like protease that is widely distributed in mammalian sperm, in terms of substrate speci®city and inhibitor susceptibility...

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Determinants of General Health Status and Specific Diseases of Elderly Women and Men: A Longitudinal Analysis for Western and Eastern Germany doc

Ngày tải lên : 22/03/2014, 13:20

... provide reliable information about a person’s experience with illness, disabilities and health behaviour (Manderbacka 1998; Simon et al 20 05) On the other hand, the correctness of the information ... of information from both waves allowed to minimise the number of missing cases in the control variables since missing information in the first survey could be substituted when the corresponding ... fertility history and migration background The reconstruction of the corresponding life-course variables was based either on information about their timing and duration or on information about the...

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Báo cáo khoa học: Advanced glycation end products and lipopolysaccharide synergistically stimulate proinﬂammatory cytokine⁄chemokine production in endothelial cells via activation of both mitogen-activated protein kinases and nuclear factor-jB pdf

Ngày tải lên : 30/03/2014, 01:20

... to block each individual signaling pathway Blockage of each individual pathway [i. e p38 with SB203580, ERK1 ⁄ with PD98059, JNK with JNK inhibitor II, and NF- jB with pyrrolidine dithiocarbamate ... Ludwig S & Wirth T (20 01) Activation of NF -kappa B via the Ikappa B kinase complex is both essential and sufﬁcient for proinﬂammatory gene expression in primary endothelial cells J Biol Chem 27 6, ... effect of MAP kinase inhibitor or NF- jB inhibitor on AGE-HSA and LPS-stimulated cell activation, HUVECs were pretreated with PD98059 (20 lm), JNK inhibitor II (50 nm), SB203580 (20 lm) (all from...

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báo cáo hóa học: " HIV-1 and IL-1b regulate astrocytic CD38 through mitogen-activated protein kinases and nuclear factor-B signaling mechanisms" ppt

Ngày tải lên : 19/06/2014, 22:20

... Tat protein is associated with an increase in both NF -kappa B binding and protein kinase C activity in primary human astrocytes J Virol 1996, 70:1384-1389 Nomura Y: NF- kappaB activation and IkappaB ... IL- 1b- activation significantly increased HIV1p24 expression in HIV-1YU -2- tranfected cells at both and days (Figure 4D, p < 0.001) Since IL- 1b is upregulated in brain macrophages and microglia and ... various binding sites for transcription factors such as activator protein- 1 and nuclear factor (NF) - B [22 ] The principal components in the signaling cascades resulting in activation of NF- B upon...

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Báo cáo hóa học: " Research Article Extraction of Protein Interaction Data: A Comparative Analysis of Methods in Use" potx

Ngày tải lên : 22/06/2014, 19:20

... Association for Computational Linguistics, pp 54–61, 20 05 J Eom and B Zhang, “PubMiner: machine learning-based text mining for biomedical information analysis, ” Genomics & Informatics, vol 2, no 2, ... 99–106, 20 04 I Donaldson, J Martin, B de Bruijn, et al., “PreBIND and Textomy—mining the biomedical literature for proteinprotein interactions using a support vector machine,” BMC Bioinformatics, ... PMID PreBIND MYC—PS2 Absent Nil 1899037 PreBIND PI (serine (or cysteine) proteinase inhibitor, clade A)—PIP (Prolactininduced protein) Absent Nil 796 821 6 4.4 Irrelevant data capture Building interaction...

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Báo cáo hóa học: "Interaction between High-Level and Low-Level Image Analysis for Semantic Video Object Extraction" docx

Ngày tải lên : 23/06/2014, 01:20

... their reliability According to [22 ], the best performance for optical flow computation in terms of reliability can be obtained by the differential technique proposed in [23 ], and by the phase-based ... frame before coding: the background information is blurred thus requiring less bandwidth while still retaining essential contextual information segmentation Each kind of segmentation exploits the ... Semantic and region partition interaction The region and the semantic partitions can be improved through interaction with one another The interaction is realized by allowing information to flow both...

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