fountain san diego california usa
Tài liệu Carnivore Ecology and Conservation A Handbook of Techniques pptx
Ngày tải lên :
17/02/2014, 17:20
... Investigations Lab, 1701 Nimbus Road, Rancho Cordova, CA 95670, USA and Wildlife Health Center, University of California, Davis, CA 95616, USA E-mail: dlclifford@ucdavis.edu Hilary S Cooley US Fish ... Center, 102 Larson Heights Road, McKinleyville, CA 95519 USA and Veterinary Genetics Laboratory, University of California, Davis, 95616 USA E-mail: mwgabriel@ucdavis.edu Jean-Michel Gaillard UMR ... Center, 102 Larson Heights Road, McKinleyville, CA 95519 USA and Veterinary Genetics Laboratory, University of California, Davis, CA 95616 USA E-mail: gmwengert@ucdavis.edu Claudia Wultsch Department...
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Carnivore Ecology and Conservation: A Handbook of Techniques pptx
Ngày tải lên :
06/03/2014, 18:20
... Investigations Lab, 1701 Nimbus Road, Rancho Cordova, CA 95670, USA and Wildlife Health Center, University of California, Davis, CA 95616, USA E-mail: dlclifford@ucdavis.edu Hilary S Cooley US Fish ... Center, 102 Larson Heights Road, McKinleyville, CA 95519 USA and Veterinary Genetics Laboratory, University of California, Davis, 95616 USA E-mail: mwgabriel@ucdavis.edu Jean-Michel Gaillard UMR ... Center, 102 Larson Heights Road, McKinleyville, CA 95519 USA and Veterinary Genetics Laboratory, University of California, Davis, CA 95616 USA E-mail: gmwengert@ucdavis.edu Claudia Wultsch Department...
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Báo cáo y học: "Pravastatin Provides Antioxidant Activity and Protection of Erythrocytes Loaded Primaqe"
Ngày tải lên :
25/10/2012, 11:40
... and thiobarbituric acid, tetraethoxypropane were provided by Sigma Chemical Co., St Louis, MO, USA Acetonitrile, methanol, isopropanol, trichloroacetic acid and sodium hydroxide were supplied ... formation in the presence of primaquine These observations are supported by the work done by Prasanthi et al., in 2005 which reported that the shape changes of erythrocytes are induced by addition ... oxidative protein modifications in the erythrocytes membrane Mech Ageing Dev 2008; 129: 572–529 Prasanthi K, Muralidhara, Rajini PS Morphological and biochemical perturbations in rat erythrocytes...
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Windows Home Server Protect and Simplify Your Digital Life
Ngày tải lên :
01/01/2014, 16:48
... Chapter 1: Introducing Windows Home Server car engines redline between five thousand and seven thousand RPM Ten thousand RPM is almost certain death for a car engine, but for many modern hard drives, ... of your hard drive is one or more thin platters, spinning at somewhere between four thousand and ten thousand revolutions per minute (RPM) For comparison, most 86251c01.qxd:ModifiedPCMagazine ... including 20 years of teaching computer classes for the University of New Mexico Mark has helped thousands of students and clients get more out of their computers in areas from DOS to Windows Vista,...
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Tài liệu Physical Activity and Women’s Health pptx
Ngày tải lên :
13/02/2014, 08:20
... most highly fit women Preand postmenopausal athletes have less atherogenic lipid profiles than sedentary or less active women matched for age and menopausal status (Rainville & Vaccaro, 1984; ... After 10 years of follow-up, premenopausal women with high levels of activity were associated with slightly increased risk of breast cancer Among postmenopausal women, however, high physical activity ... 1989) A strong inverse relationship between LTPA and death from stroke was observed in postmenopausal women (Paganini-Hill et al., 1988) Those who were physically active less than 30 minutes per...
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Tài liệu Time trends in leisure time physical activity and physical fitness in elderly people: 20 year followup of the Spanish population national health survey (1987-2006) docx
Ngày tải lên :
14/02/2014, 06:20
... estadisticas/encuestaNacional/encuesta2006.htm] 39 Ministerio de Sanidad y Consumo: Spanish National Health Survey 19872003 Madrid, Spain: Ministerio de Sanidad y Consumo; 2004 [http://www msps.es/estadEstudios/estadisticas/estadisticas/microdatos/ ... LTPA in both genders, which is in agreement with previous studies conducted in Australia [16] and USA [29] Gallant and Dorn [42] reported that marital status showed an influential element in men’s ... ahead Lancet 2009, 374:1196-1208 Prohaska T, Belansky E, Belza B, Buchner D, Marshall V, McTigue K, Santariano W, Wilcox S: Physical activity, public health, and aging: Critical 24 25 issues and research...
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Tài liệu Báo cáo khoa học: Secondary substrate binding strongly affects activity and binding affinity of Bacillus subtilis and Aspergillus niger GH11 xylanases docx
Ngày tải lên :
14/02/2014, 19:20
... The obtained graphs were fitted in graphpad prism, version 3.03 (GraphPad Software Inc., San Diego, CA, USA) , with a ‘one site binding model’ that results in an overall dissociation constant (Kd): ... mutants were constructed using a QuikChange site-directed mutagenesis kit (Stratagene, La Jolla, CA, USA) The template DNA and oligonucleotide primers used are shown in Table S1 XBS XBS and its mutant ... measurement of E280 with a Nanodrop-1000 spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA) using molar extinction coefficients calculated with protparam software (http://expasy.org/tools/protparam.html)...
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Tài liệu Báo cáo khoa học: Processing, catalytic activity and crystal structures of kumamolisin-As with an engineered active site pptx
Ngày tải lên :
19/02/2014, 07:20
... Toyobo, Osaka, Japan The plasmid pScpA, which is a derivative of pET15b (Novagen, Madison, WI, USA) , was constructed as described previously [15] and was used for the expression of the wild-type ... D164N) and pScpA, respectively, using a QuickChange Mutagenesis Kit (Stratagene, La Jolla, CA, USA) according to the manufacturer’s guidelines Additional plasmids, pScpA ⁄ E78A ⁄ D164N and pScpA ... sequencing on both strands using a Dye-terminator Cycle Sequencing Kit (Beckman Coulter, Fullerton, CA, USA) with a CEQ 2000 DNA analysis system (Beckman Coulter) Expression of wild-type and mutated kumamolisin-As...
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Tài liệu Báo cáo khoa học:Symmetric fluoro-substituted diol-based HIV protease inhibitors Ortho-fluorinated and meta-fluorinated P1/P1¢-benzyloxy side groups significantly improve the antiviral activity and preserve binding efficacyy docx
Ngày tải lên :
19/02/2014, 16:20
... at °C The supernatant was applied to a POROS QTM column (Perspective Biosystems, Cambridge, CA, USA) The flowthrough fraction was collected and diluted to a final protein concentration of 0.3 mgÆmL)1 ... MES, pH 6.5, mM dithiothreitol, mM EDTA), applied to a POROS HS column (Centricon, Billerica, CA, USA) , and eluted with a linear gradient of 0–0.6 M NaCl in MES buffer The pooled fractions were ... Active human immunodeficiency virus protease is required for viral infectivity Proc Natl Acad Sci USA 85, 4686–4690 Roberts, N.A., Martin, J.A., Kinchington, D., Broadhurst, A.V., Craig, J.C., Duncan,...
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Tài liệu Báo cáo khoa học: Changes in rat liver mitochondria with aging Lon protease-like activity and N e-carboxymethyllysine accumulation in the matrix doc
Ngày tải lên :
20/02/2014, 11:20
... Proc Natl Acad Sci USA 94, 11168–11172 18 Yan, L.J & Sohal, R.S (1998) Mitochondrial adenine nucleotide translocase is modified oxidatively during aging Proc Natl Acad Sci USA 95, 12896–12901 ... Hagen, T.M & Ames, B.N (1994) Oxidative damage and mitochondrial decay in aging Proc Natl Acad Sci USA 91, 10771–10778 16 Sastre, J., Pallardo, F.V., Garcia de la Ascunsion, J & Vina, J (1999) Mitochondria, ... associated with aging is reduced by dietary restriction of protein or calories Proc Natl Acad Sci USA 89, 9112–9116 Giardino, I., Edelstein, D & Brownlee, M (1994) Nonenzymatic glycosylation in...
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Tài liệu Báo cáo khoa học: Endotoxic activity and chemical structure of lipopolysaccharides from Chlamydia trachomatis serotypes E and L2 and Chlamydophila psittaci 6BC pdf
Ngày tải lên :
20/02/2014, 23:20
... Ion Cyclotron Resonance (ESI-FTICR) mass spectrometer (APEX II, Bruker Daltonics, Billerica, MA, USA) equipped with a Tesla actively shielded magnet and an Apollo ion source Capillary skimmer dissociation ... and human TLR4 were a kind gift from P Nelson, Fred Hutchinson Cancer Research Center, Seattle, USA and were subcloned into pREP9 (Invitrogen) The human MD-2 expression plasmid was a kind gift ... with an expression vector for human CD14 (pCDNA3huCD14, a kind gift of D Golenbock, Worcester, MA, USA) or with the vector without insert as control Stable transfected cell lines (HEKvec and HEK-CD14)...
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Báo cáo khoa học: Relationship between functional activity and protein stability in the presence of all classes of stabilizing osmolytes ppt
Ngày tải lên :
07/03/2014, 00:20
... preparations of RNase-A (type III-A) were purchased from Sigma Chemical Company (St Louis, MO, USA) d-glucose, d-fructose, d-galactose, d-sucrose, d-raffinose, d-stachyose, glycine, l-alanine, ... hydration and the modulation of biochemical reactions by solvent components Proc Natl Acad Sci USA 99, 9721–9726 FEBS Journal 276 (2009) 6024–6032 ª 2009 The Authors Journal compilation ª 2009 ... affecting counteraction by methylamines of urea effects on aldose reductase Proc Natl Acad Sci USA 96, 6517–6522 21 Poddar NK, Ansari ZA, Singh RK, Moosavi-Movahedi AA & Ahmad F (2008) Effect...
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Báo cáo khoa học: Haptoglobin binds the antiatherogenic protein apolipoprotein E – impairment of apolipoprotein E stimulation of both lecithin:cholesterol acyltransferase activity and cholesterol uptake by hepatocytes pdf
Ngày tải lên :
07/03/2014, 00:20
... obtained from Chemicon (Millipore, Billerica, MA, USA) Monoclonal mouse anti-human ApoE IgG was purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA) The ApoA-I mimetic peptide P2a was synthesized ... (Billerica, MA, USA) were used The dye reagent of Bio-Rad (Bio-Rad, Hercules, CA, USA) was used for protein titration StartingBlock blocking buffer was from Thermo Fisher Scientific (Rockford, IL, USA) Polystyrene ... value ± standard error of the mean (SEM) The program graph pad prism (Graph Pad Software, San Diego, CA, USA) was used to obtain trend curves and to perform regression analysis or t-tests Acknowledgements...
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Báo cáo khoa học: Simultaneous improvement of catalytic activity and thermal stability of tyrosine phenol-lyase by directed evolution ppt
Ngày tải lên :
07/03/2014, 00:20
... toebii TPL were constructed using the Build Model module from Discovery Studio (Accelrys, San Diego, CA, USA) The model with the best loop conformations was then selected using the Profiles-3D verification ... were obtained from Musashino Shoji (Tokyo, Japan) and Fluka (Seelze, Germany), respectively, and yeast extract and bacto-casitone were purchased from BD (Franklin Lakes, NJ, USA) The other chemicals, ... from Sigma-Aldrich (St Louis, MO, USA) The restriction endonucleases, T4 DNA ligase and Vent DNA polymerase were purchased from New England Biolabs (Beverly, MA, USA) , and the Taq DNA polymerase...
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Báo cáo khoa học: Hyperthermophilic enzymes ) stability, activity and implementation strategies for high temperature applications pot
Ngày tải lên :
07/03/2014, 05:20
... domains archaea, bacteria, and eucarya Proc Natl Acad Sci USA 87, 4576–4579 Woese CR (1998) The universal ancestor Proc Natl Acad Sci USA 95, 6854–6859 Miller SL & Lazcano A (1995) The origin ... Biol 336, 203–212 Patil AJ, Muthusamy E & Mann S (2004) Synthesis and self-assembly of organoclay-wrapped biomolecules Angew Che Int Ed 43, 4928–4933 Patil AJ, Muthusamy E & Mann S (2005) Fabrication ... A, Raven NDH, Sharp RJ, Bartolucci S, Rossi M, Cannio R, Lebbink J, van der Oost J, deVos WM & Santos H (1997) Stabilization of enzymes against thermal stress and freeze-drying by mannosylglycerate...
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Báo cáo khoa học: The Vps4 C-terminal helix is a critical determinant for assembly and ATPase activity and has elements conserved in other members of the meiotic clade of AAA ATPases pdf
Ngày tải lên :
07/03/2014, 05:20
... (Hanover, MD, USA) Poly(vinylidene difluoride) membrane was from Millipore (Bedford, MA, USA) Goat polyclonal anti-(yeast Vps4p) IgG was from Santa Cruz Biotechnology (Santa Cruz, CA, USA) and rabbit ... anti-(rabbit IgG) were from Sigma-Aldrich (St Louis, MO, USA) Horseradish peroxidase-conjugated rabbit anti-(goat IgG) was from Zymed (San Francisco, CA, USA) Horseradish peroxidaseconjugated goat anti-(mouse ... 658 nm), which provides scattering measurements at up to 16 angles (Wyatt Technology, Santa Barbara, CA, USA) , combined with an Optilab rEX refractive index detector (Wyatt Technology) MALLS...
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Báo cáo khoa học: Engineering and characterization of human manganese superoxide dismutase mutants with high activity and low product inhibition potx
Ngày tải lên :
07/03/2014, 11:20
... randomized library of protein variants based on the Q143A hMnSOD template One hundred and fifty thousand QC774 transformants were screened on 40 M63 minimal media agar plates containing lm paraquat ... injury, and perinatal death in mitochondrial superoxide dismutase-deficient mice Proc Natl Acad Sci USA 93, 9782–9787 Li YB, Huang TT, Carlson EJ, Melov S, Ursell PC, Olson TL, Noble LJ, Yoshimura ... dismutase expression suppresses the malignant phenotype of human melanoma cells Proc Natl Acad Sci USA 90, 3113–3117 12 Li JJ, Oberley LW, St Clair DK, Ridnour LA & Oberley TD (1995) Phenotypic changes...
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Báo cáo khoa học: Alpha-fetoprotein antagonizes X-linked inhibitor of apoptosis protein anticaspase activity and disrupts XIAP–caspase interaction ppt
Ngày tải lên :
07/03/2014, 12:20
... caspase activity Caspase assays were performed with active recombinant caspase-3 (Alexis Corp., San Diego, CA), recombinant fulllength rhXIAP (R&D Systems, Minneapolis, MN), purified AFP, and HSA ... polyclonal rabbit anti-AFP [28], normal rabbit IgG (Sigma), or with rabbit anti-(caspase 3) (Santa Cruz, Santa Cruz, CA) for h with a gentle mixing at °C Thereafter, 40 lL of protein A–Sepharose ... anti-AFP IgG [28] or goat anti-XIAP IgG (R&D Systems, Minneapolis, MN), or anti-(caspase 3) IgG (Santa Cruz) and then incubated with donkey anti-(rabbit IgG) (Amersham Pharmacia Biotech) or anti-(goat...
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Báo cáo khoa học: Amino acids at the N- and C-termini of human glutamate carboxypeptidase II are required for enzymatic activity and proper folding pptx
Ngày tải lên :
07/03/2014, 15:20
... C-terminally tagged construct was generated similarly to the 44/750 variant The only exception was usage of the reverse primer (complementary to the C-terminal part of GCPII) that was devoid Ó FEBS ... 40 · 106 cells per mL, sonicated three times (20 s each, 10 lm amplitude) on ice (Soniprep 150; Sanyo), and subjected to centrifugation at 15 000 g for 10 The supernatant fraction is referred ... 4-hydroxy5-iodo-3-nitrophenylacetylLeu-Leu-leucinal-vinyl sulfone require enzymatically active proteasomes for continued survival Proc Natl Acad Sci USA 98, 513–518 28 Geier, E., Pfeifer, G., Wilm, M., Lucchiari-Hartz, M., Baumeister, W., Eichmann,...
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