formation and fate of nephrotoxic and cytotoxic glutathione s conjugates cysteine conjugate amp 946 lyase pathway
Tài liệu Báo cáo Y học: Characterization of an omega-class glutathione S-transferase from Schistosoma mansoni with glutaredoxin-like dehydroascorbate reductase and thiol transferase activities pptx
Ngày tải lên :
21/02/2014, 01:21
... blot analysis was performed using S mansoni cercariae purified DNA as described [26] Alignments and phylogenetic analysis of S mansoni GSTs Sm26GST, Sm28GST and SmGSTO amino acid sequences were aligned ... regions of SmGSTO presented significant divergences from the known GSTO, at the primary structure level Indeed, the GSTO class represents a particular class of the GST superfamily which possesses specific ... Construction of a homology model of SmGSTO SmGSTO structure was built using the human GSTO 1–1 structure as template in the SWISS-MODEL modeling Ó FEBS 2002 Omega-class GST from Schistosoma mansoni...
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Báo cáo khoa học: Purification and cloning of a Delta class glutathione S-transferase displaying high peroxidase activity isolated from the German cockroach Blattella germanica pptx
Ngày tải lên :
23/03/2014, 09:21
... at least six classes of cytosolic GSTs in insects [2] The majority of GSTs are in the Delta and Epsilon classes, and the remaining enzymes are in the Omega, Sigma, Theta and Zeta classes The ... of GSTs are of particular importance to insects, because they not possess selenium-dependent glutathione peroxidase A survey of reported GST-mediated peroxidase activity across insect species ... specificities of purified cockroach GST compared with those of other insect GSTs Values are the means ± SE from three separate experiments Substrate specificities of Delta class GSTs from D melanogaster...
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Tài liệu Environmental impacts of petroleum production: Fate of inorganic and organic chemicals in produced water from the Osage-Skiatook Petroleum Environmental Research sites, Osage County, Oklahoma doc
Ngày tải lên :
14/02/2014, 03:20
... shale, siltstone, and sandstone Thicker resistant sandstone units typically form the hill crests Hill slopes are underlain by shale, siltstone, and thin sandstone beds The geologic and climatic settings ... releases of produced water and petroleum and the improper disposal of produced water are national issues that concern managers of Federal, and State lands, as well as oil and gas producers and lessees, ... American Society for Testing and Materials, ASTM standards on assessment and remediation of petroleum release sites, 126 p., ASTM, Committee E-50 on Environmental Assessment (1999) Wilson, M.J and...
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Báo cáo khoa học: Amyloid oligomers: formation and toxicity of Ab oligomers ppt
Ngày tải lên :
06/03/2014, 09:22
... our findings also suggest the possibility that the suppression of protein aggregation may cause the formation of toxic oligomeric species, which is consistent with previous results showing that ... 2010 FEBS 1349 Formation of toxic Ab oligomers M Sakono and T Zako least possess the structural characteristics of fibrils Thus, it is plausible that the fibrillar oligomer might represent fibril ... mechanisms of formation may also differ for extracellular and intracellular oligomers In this section, we discuss possible formation mechanisms of extracellular and intracellular Ab oligomers, and also...
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Báo cáo khoa học: Replacement of two invariant serine residues in chorismate synthase provides evidence that a proton relay system is essential for intermediate formation and catalytic activity docx
Ngày tải lên :
07/03/2014, 05:20
... acid residues, the active site of chorismate synthase features two strictly conserved (99.5% of 400 sequences aligned) serine residues located near the substrate on the opposite site of the isoalloxazine ... structure-based mutagenesis study to test mechanistic proposals In our first study we demonstrated that the invariant histidine residues (His17 and His106) function as general acids in the active site, ... molecule (wat 1) is held by both serine side chains (in the reported structure of S pneumoniae chorismate synthase, these positions are designated as Ser9 and Ser132, [10]) close to the C(3)–oxygen,...
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The fate of stormwater-associated bacteria in constructed wetland and water pollution control pond systems potx
Ngày tải lên :
15/03/2014, 23:20
... expressed as cfu 100 g wet sedimentÀ1 Sediment microcosms Sediment samples from the inlet and outlet ends of each system (PP1, PP3, WC1 and WC3) were used for sediment microcosms For each sample, ... Stormwater enters the system via two inlets (PI1 and PI2) and there is a single outlet (PO) Sampling locations for in¯ow and out¯ow samples, and for sediment and water column samples are indicated ... rates were signi®cantly greater in the absence DISCUSSION In natural aquatic systems the adsorption of allochthonous micro-organisms to sand, silt and clay particles which then undergo physical sedimentation...
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Influences of the si(1 1 3) anisotropy on ge nanowire formation and related island shape transition
Ngày tải lên :
16/03/2014, 15:34
... the stress of the island, but also on the substrate stiffness and the island shape Unfortunately, Tersoff and Tromp did not pay attention to the substrate anisotropy in stiffness but assumed the same ... islands or a combination of dot-like and dislocated islands Volume measurements of each type of island demonstrate that the shape transition of the islands proceeds via mass transport Interestingly, ... the islands and the substrate surface tends to drive atoms to diffuse onto the islands, forming a more energetically stable island shape with less elongation Island shape formation of Ge on Si(1...
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Báo cáo khoa học: Mercury(II) binding to metallothioneins Variables governing the formation and structural features of the mammalian Hg-MT species pptx
Ngày tải lên :
16/03/2014, 18:20
... presence of different chromophores in the same species including Zn and/ or Hg as metal ions and SCys and/ or Cl– as ligands; (b) the absence of well-established relationships between most of the ... (Figures S1 5 S1 7) are consistent with the reaction pathways shown in Scheme Comparison of the three sets of data recorded at pH (Scheme 3A) reveals that the Hg:bMT stoichiometry of the species does ... to SCys at this pH Metal solutions Glassware and solutions used in metal ion-binding studies were prepared as described [20] A Riedel-de Haen atomic ¨ absorption spectrometry Hg2+ standard of...
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Báo cáo khoa học: The crystal structure of annexin Gh1 from Gossypium hirsutum reveals an unusual S3 cluster Implications for cellulose synthase complex formation and oxidative stress response potx
Ngày tải lên :
17/03/2014, 03:20
... molecular basis of oxidative stress response by these proteins Similarly, an annexin from M sativa was reported to act as stress-response protein [7] and several mammalian annexins are also known ... proteins are components of plasma membrane-bound CesA complexes with sixfold symmetry and usually referred to as rosettes Current models assume that the active site of plant CesA proteins is formed ... immediately shows that binding sites in domains II, III and IV are either distorted or the access of a cation to the site is blocked by the presence of a side chain of a basic residue In case of the...
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Transport and fate of persistent toxic organic chemicals in aquatic ecosystems: the Niagara River to St Lawrence River Estuary example pptx
Ngày tải lên :
22/03/2014, 14:20
... sediments Bottom sediments of lakes and impoundments are often major sinks and sites of eventual burial of PTOCs Distribution patterns of PTOCs in lake bottom sediments provide clues to sites of maximum ... ecosystems The best way to resolve such pathways and to have confidence in future levels of exposure or predictions of rates of ecosystem recovery is by collection and analysis of multi-media samples ... released Clearly, most of these factors which affect the partitioning of PTOCs onto particulates (suspended solids/sediments) and thus their transport and fate in aquatic ecosystems are site...
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Báo cáo khoa học: Metabolism of N-methyl-amide by cytochrome P450s Formation and characterization of highly stable carbinol-amide intermediate pdf
Ngày tải lên :
22/03/2014, 16:20
... distinguished 14 C-specic radioactive isotopomers have been fruitfully used for this purpose This set of experiments shows that metabolism occurs specically on the N-Me groups of TTX residue and ... assistance of a water molecule In a nonaqueous media, deformylation should slowly occur via this transition state Discussion In vitro incubations of TTX and some of its analogs, using microsomal liver ... metabolites starting with both natural and 14C-radiolabeled substrates using HPLC; (b) structural identication of metabolites by HPLC MS and MS-MS, and NMR spectroscopy; (c) demonstration of the...
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Báo cáo khoa học: Purification and properties of the glutathione S-transferases from the anoxia-tolerant turtle, Trachemys scripta elegans pdf
Ngày tải lên :
23/03/2014, 15:20
... mass of some yeast (Y lipolytica) GSTs, however, is 110 kDa [32] Thus GSTs can vary widely in their subunit size The larger molecular mass of turtle GSTs may arise as a result of post-translational ... directed against thiol groups, is a nonspecific inhibitor of all classes S- hexylglutathione shows highest to lowest inhibition of a, l and p GST isozymes, respectively [38] Both Peak and GSTs showed ... class which typically shows only minor activity towards cumene hydroperoxide The classification of GSTs also depends on their responses to substrates and inhibitors Like all GSTs, turtle liver GSTs...
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Báo cáo khoa học: Limited proteolysis of Escherichia coli cytidine 5¢-triphosphate synthase. Identification of residues required for CTP formation and GTP-dependent activation of glutamine hydrolysis potx
Ngày tải lên :
23/03/2014, 17:22
... synthases Fig SDS/PAGE analysis of trypsin-catalysed cleavage of recombinant wild-type CTPS in the absence and presence of ligands For each gel: lane contains molecular mass standards and lanes 26 ... trypsin-catalysed cleavage Interestingly, in the Fig SDS/PAGE analysis of trypsin-catalysed cleavage of mutant CTP synthases in the absence and presence of ligands (A) Lane 1, molecular mass standards; ... C-terminus (GAT domain) of CTP synthases For the protein sequences shown, invariant residues (*), conservative substitutions (:), and semiconservative substitutions (.) are indicated The two residues...
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Báo cáo khoa học: Transfection with 4-hydroxynonenal-metabolizing glutathione S-transferase isozymes leads to phenotypic transformation and immortalization of adherent cells pdf
Ngày tải lên :
30/03/2014, 13:20
... concentration of 4-HNE but also changes in the homeostasis of 4-HNE and its metabolites that provides the mechanistic basis for the transformation This possibility needs to be explored in future studies ... aggregates All steps of the sample preparation were performed at °C, and the samples kept on ice until injected into cells Samples of recombinant mGSTA4-4, DmGSTD1-1, hGSTA1-1 and hGSTP1-1 used for ... conjugation of 4-HNE to GSH results in the formation of GS-HNE An increase in the level of GS-HNE may also be a trigger of transformation Previous studies have shown that overexpression of 4-HNEmetabolizing...
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Báo cáo khoa học: Purification and partial characterization of seven glutathione S -transferase isoforms from the clam Ruditapes decussatus pptx
Ngày tải lên :
31/03/2014, 09:20
... conditions as given in Fig Ó FEBS 2002 Purification of R decussatus GST isoforms (Eur J Biochem 269) 4363 Table Biochemical characteristics of R decussatus GST isoforms Results are expressed as means ... alpha and pi class consensus sequences from mammals, respectively The N-terminal sequence of subunit of R decussatus exhibits a high similarity with the corresponding consensus mu GST class from ... antisera specific of mammalian pi-class GST Studies of aquatic species such as salmonids [26], Bufo bufo [27] and M edulis [28], showed that their GSTs belong to the pi class The GST isoforms 1-1...
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Báo cáo Y học: Selection of effective antisense oligodeoxynucleotides with a green fluorescent protein-based assay Discovery of selective and potent inhibitors of glutathione S-transferase Mu expression doc
Ngày tải lên :
31/03/2014, 15:20
... tested AS-ODNs) DISCUSSION Most of currently available screening assays for the selection of effective AS-ODNs are based on cell-free assays, e.g RNAse H digestion screens and oligonucleotide scanning ... isoform specificity of AS-5 and AS-10 in the EGFP assay, compared to the GST assay In summary, we selected several effective antisense ODNs against rat GSTM1 and GSTM2 from a set of 15 PS-ODNs ... C-terminal fusion constructs The newly developed screening assay was used for the selection of effective AS-ODNs against rat GSTM1 and GSTM2 out of a set of 15 PS-ODNs Some ODNs were designed to specifically...
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báo cáo hóa học:" Generation in vivo of peptide-specific cytotoxic T cells and presence of regulatory T cells during vaccination with hTERT (class I and II) peptide-pulsed DCs" pot
Ngày tải lên :
18/06/2014, 15:20
... thrombosis, sepsis) related to apheresis line insertion, which required hospitalization One of these patients died from septic complications, as a result of the of apheresis line insertion Discussion ... as illustrated in Figure 9B This was a surrogate measure of in vitro cytotoxicity against naturally processed peptides of hTERT, as SCC-4 is known to inherently express hTERT peptides on its surface ... CD8+T cell responses (mean +/- SD) to class I ± II hTERT pulsed DCs Tetramer+ CD8+T cell responses (mean +/- SD) to class I ± II hTERT pulsed DCs Vaccinations with DCTs pulsed with class I hTERT...
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Fate of Pharmaceuticals in the Environment and in Water Treatment Systems - Chapter 1 pot
Ngày tải lên :
18/06/2014, 16:20
... communities to fund studies or request proposals that address the presence, fate, and effects of PPCPs in aquatic systems The release of the first comprehensive reconnaissance of pharmaceuticals and ... presence and concentrations of these compounds These studies of the presence and concentrations of PPCPs have led to multiple focused studies that address the need to understand the transformation ... human and aquatic life As the results of these diverse studies converge, and a more detailed understanding of the processes affecting the concentrations of PPCPs and their degradates develops, it...
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Fate of Pharmaceuticals in the Environment and in Water Treatment Systems - Chapter 2 docx
Ngày tải lên :
18/06/2014, 16:20
... GC-MS and LC-(+)ESI-MS/MS >70 estrogens SW, WW SPE: acetone GC-IT-MS/MS estrogens, conjugated estrogens, and progestrogens Water — ICM11 WW SPE: ENV+, pH 2.8; MeOH GC-MS, LC-MS and LC-MS/MS [Optimization ... components for access to the droplet surface to the gas-phase emission A feasible solution to address this issue is to use standard addition, but this is time consuming and cost intensive Another ... surface and wastewaters using an SPE (Oasis HLB) method and LC-(+)ESI-MS/MS and LC-ESI-IT-MS This was presented as a “nonintrusive” © 2008 by Taylor & Francis Group, LLC Advances in the Analysis of...
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Fate of Pharmaceuticals in the Environment and in Water Treatment Systems - Chapter 3 pdf
Ngày tải lên :
18/06/2014, 16:20
... Chemistry, in press 14 Lee, L .S. , Strock, T.J., Sarmah, A.K., and Rao, P .S. C., 2003 Sorption and dissipation of testosterone, estrogens, and their primary transformation products in soils and sediment ... each type of SPE setup was performed on a soil extract, and the corresponding SPE setup is shown in Figure 3.2 This indicates that the use of tandem SPE for soil samples removes most of the coextracted ... instrumentation 3.4.1 SOLID PHASE EXTRACTION SPE is used to separate compounds in a sample based on their polarities and solubilities in specific solvents and is based on principles similar to those of liquid...
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