foodborne illnesses and allergens

... airways and even reach the lower respiratory tract [31,32] In vitro studies have shown that Lol p and Bet v 1, which are the prevalent or majority allergens of Lolium (a gramineous species) and birch, ... exposed individuals, and also upon allergens - directly increasing exposure to the latter, their concentration and/ or biological allergenic activity However, at present and at the general population ... health threat In the late XIX and early XX Century, many norms came into effect to control smog (air pollution resulting from the burning of coal) in both England and the United States Such legislation...

Ngày tải lên: 23/03/2014, 02:20

... scallops, and mussels) concentrate the toxin associated with "red tide." Naturally poisonous plants and animals include certain mushroom species and certain tropical fish (puffer type fish and ocean ... (vegetables and fruits) which have been improperly processed Ingestion of inadequately cooked toxin-containing food leads to nerve toxicity manifested by symptoms of weakness, headache, and dizziness, and ... botulism have also resulted from home-canned meats and fish, smoked fish, and improperly prepared commercial products, such as vichyssoise soup and potpies (4) Toxins produced in food contaminated...

Ngày tải lên: 25/01/2016, 18:52

Ngày tải lên: 28/08/2016, 10:59

Ngày tải lên: 28/08/2016, 17:43

... 39KD, 83KD and the 93KD bands are the species-specific ones, but appear later or may not appear at all You should see at least the 41KD and one of the specific bands 55KD, 60KD, 66KD, and 73KD ... heat, ultrasound and simple range of motion exercises to relieve discomfort and promote better sleep and flexibility Ice (vasoconstriction) and electrical stimulation (muscle spasm and trauma) should ... include palpitations, lightheadedness and shakiness especially after exertion and prolonged standing, heat intolerance, dizziness, fainting (or near fainting), and an unavoidable need to sit or lie...

Ngày tải lên: 05/03/2014, 10:20

... Enterovirus is resistant to alcohol and phenolic disinfectants; Linen and Waste from patients suffering from HFMD should be handled with care, and wash hands after handling 7.5 Advice given to the ... Clinical features and risk factors of pulmonary oedema after enterovirus-71-related hand, foot, and mouth disease Lancet 1999;354:1682–86 Alexander Jp Jr, Baden L, Pallansch MA, Anderson LJ, Enterovirus ... B2, B5 and EV71 HFMD is sometimes confused with foot -and- mouth disease of cattle, sheep, and swine The latter is caused by a virus Aphthovirus which belong to the family of Picornaviridae .and will...

Ngày tải lên: 05/03/2014, 12:20

... a singly EF-hand-paired dimer may form, as shown here, via strands b1 and b1¢; possible alternative dimers would involve strands b2 and b2¢, b1 and b2¢, or b2 and b1¢ Singly EF-hand-paired dimers ... p [41], we propose that dimerization is mediated by intermolecular EF-hand pairing via strands b1 and b2¢ and strands b2 and b1¢ For this dimerization to occur, we propose the existence of an ... antiparallel b-strands, and thus crosslinks the first Ca2+-binding EF-hand (shown in red) with the second EF-hand (blue) We carried out experiments to test both the temperature and time dependence...

Ngày tải lên: 06/03/2014, 11:20

... identity between Hol l and CP2 was  22%, between Phl p and CP1 as well as CP3 19%, and between Phl p and CatB  15% Moreover, the putative active-site amino acid Cys72 of Phl p and Hol l is very ... cysteine residues and other essential amino acids are numbered Areas of high similarity exist around Cys69 and Cys72 and around Trp186, Trp193 and Trp197 The positions of Cys41, 57, 69 and 72 are especially ... Promega, Madison, WI, USA), and sequenced To construct the mutated form, fragments were released using the restriction enzymes SmaI and SnaBI or EcoRI and SnaBI, respectively, and both ligated in pBS,...

Ngày tải lên: 08/03/2014, 22:20

... touching cooked foods and surfaces used for cooking or eating It’s also important to wash raw foods in sanitary water and wash hands, equipment, and cooking and food-handling surfaces; and keep food ... things like fishcakes and 165°F for stuffed fish It’s important to wash raw foods in sanitary water and to wash hands, equipment, and cooking and food-handling surfaces, and to keep food refrigerated ... Washing your hands before and after handling food, and in between handling different foods, is one of the most important steps you can take Do the same with equipment, utensils, and countertops...

Ngày tải lên: 24/03/2014, 04:20

... Regulate Spleen and Stomach Participants Look Back to Treat Five Strains and Seven Impairments Sway Head and Buttocks to Expel Heart-Fire Pull Toes with Both Hands to Reinforce Kidney and Waist Clench ... Clench Fists and Look with Eyes Wide Open to Build up Strength and Stamina Rise and Fall on Tiptoes to Dispel All Diseases Qigong is a complete exercise for both the body and the mind Li and Sun (1997) ... motion and mobility, and improve affect and mood in a group of 70 elderly patients In a study using aerobic exercise as the treatment protocol among a group of 81 healthy elderly aged between 60 and...

Ngày tải lên: 28/03/2014, 19:21

... fluorescence was monitored with excitation and emission wavelengths at 275 and 310 nm, with and nm bandwidth, respectively Buffer contributions were corrected and inner filter effect was negligible, ... importantly, the ability of these allergens to bind and transfer lipids is demonstrated in the present study using both natural and fluorescently labeled ligands Results Molecular model comparison ... Tyr81 and Arg46 are shown as a ball and stick model Surface of the cavities from ns-LTPmaize–palmitate complex (1mzm.pdb) (D), Par j (E) and Par j (F) models, unligated ns-LTPrice (1rzl.pdb) (G) and...

Ngày tải lên: 30/03/2014, 02:20

... in AtOle e and AtOle e allergens Furthermore, inhibition analyses of the binding to IgG and IgE between both recombinant forms of each allergen (i.e AtOle e and rOle e 3, or AtOle e and rOle e ... digestion and MS analysis Bands of interest were manually excised from SDS ⁄ PAGE gels (see below), alkylated and digested with trypsin [37] Bands were shrunk with 100% acetonitrile and dried ... which contain two EF-hand motifs and are responsible 4426 for cross-reactivity among pollens [17] Ole e is a 19 kDa protein with four EF-hand motives [18] Putative homologous allergens to Ole e...

Ngày tải lên: 30/03/2014, 10:20

... 12, and next IL in tissues Also the type of stimulated TLR is important In the situation of allergic disease and Th2-sensitive response, TLR and are stimulated, and on DCs Delta and Jagged ligands ... Németh, ˛ and András Nagy Chapter Methods for Detection of Food Allergens 83 Lucjan Jedrychowski, André H Penninks, Maciej Kaczmarski, ˛ Beata Cudowska, Elzbieta Korotkiewicz-Kaczmarska, Andrea ... Fátima Ferreira, and Martin Himly Chapter Recombinant Food Allergens and Their Role in Immunoassay and Immunotherapy 169 Karin Hoffmann-Sommergruber, Christina Oberhuber, and Merima Bublin...

Ngày tải lên: 28/05/2014, 14:08

... symptoms and syndromes in the etiology of mental illnesses In Chapter 1, Andrew Soundy, Thomas Kingstone, and Pete Coffee indicate how physical activity can benefit individuals with severe mental illnesses ... Crone (2007), Fogarty and Happell (2005), Faulkner and Sparkes (1999), Carter-Morris and Faulkner (2003), Carless and Douglas (2004), and Crone and Guy (2008) Our exclusion criteria should not ... used tables and grids to help in this and to display themes and concepts (Atkins et al., 2008; Campbell et al., 2003) In other words, we summarised and wrote commentary on the results and discussion...

Ngày tải lên: 27/06/2014, 16:20

... Cytokine, IgE and tryptase ELISAs Lung homogenate and BAL fluid protein levels were assessed as previously described [14] CCL11, CCL2, IL-12 and IL-13 primary and secondary antibodies and standards ... IgE biotinylated antibody, and mouse IgE standard (Pharmingen, San Diego, CA) Standards and samples were diluted in dilution buffer containing 10% Casein, 0.05% Tween 20 and 0.0001% BSA in 1× PBS ... increased bronchoconstriction and mucin production [16,17] Both C5a and C3a have receptors on mast cells and basophils and can activate these cells to release histamine and leukotrienes [18-21] Therefore,...

Ngày tải lên: 12/08/2014, 16:20

... Figure and MADLI reflectron TOF of tryptic peptides derived from band (Lolium perenne) and band 12 (Cynodon dactylon) SDS/PAGE SDS/PAGE and MADLI reflectron TOF of tryptic peptides derived from band ... perenne) and band 12 (Cynodon dactylon) A Pollen diffusates (~ 20 µg: lane Kentucky blue grass, lane rye grass and lane Bermuda grass) were separated using SDS/PAGE, stained with CBB and labelled bands ... identification and differentiation of allergen isoforms and of post-translational modifications Our data strongly suggest that, analogous to house dust mite and other allergens, pollen allergens may...

Ngày tải lên: 13/08/2014, 13:20

... RRIM600 Latex allergens MNA Glove Glove Latex allergens Figure IgE reactivity of HCW and SB patients to latex and glove extract antigens IgE reactivity of HCW and SB patients to latex and glove extract ... 6, 7, 8, 9, and 10 were cloned from cDNA libraries and Hev b 1, 3, 5, and were expressed in the Medical College of Wisconsin laboratory (VPK), while Hev b 7, 8, 9, and 10 were cloned and expressed ... profile of latex allergens Allergens (10 µg) were subjected to electrophoresis in 12% SDS-Gel and stained with Coomassie brilliant blue – Molecular weight standards; 2–12 – Hev b to 10 and Hev b 13;...

Ngày tải lên: 13/08/2014, 13:22

... cytokines and facilitate transport of allergens across cultured epithelial cell layers [2,3,7,13] Der p and Der p lack a clear-cut protease activity, but are major IgE binding proteins [14] and of ... http://www.clinicalmolecularallergy.com/content/4/1/5 Benelux, The Netherlands) Affinity chromatography purified natural Der p1 and the recombinant allergens (Der p [16,17], Der p [18], and processed Der p [19]) were a generous ... (Groningen, The Netherlands) The retrograde analogues of PAR-1 and PAR-2 were used to show specificity of the PAR-1 and PAR-2 agonists Table 1: Effects on cell shrinking and desquamation of house...

Ngày tải lên: 13/08/2014, 13:22

... 1), 3), 5) and 7), and pellet fractions, lanes 2), 4), 6), and 8) Expression of Bb-Eno1, lanes 1) and 2), Bb-f2, lanes 3) and 4), Bb-Ald, lanes 5) and 6), and Bb-Hex, lanes 7) and 8) and K-T (Fig ... BL21 harboring pRARE and the indicated expression plasmid constructs; lanes 1) and 2); pET41a:Bb-Eno1, lanes 3) and 4) pET41a:Bb-f2, lanes 5) and 6) pET41a:Bb-Ald, lanes 7) and 8) pET41a:Bb-Hex ... mannan and 46-48 kD allergens of Saccharomyces cerevisiae and Candida albicans Clin Exp Allergy 1993, 23:179-184 Rhyner C, Weichel M, Fluckiger S, Hemmann S, Kleber-Janke T, Crameri R: Cloning allergens...

Ngày tải lên: 13/08/2014, 13:22

... Classification of dust mite allergens (adapted and modified from www.allergome.org and (Thomas, Smith et al 2002) The allergens are grouped based on their function and sequence similarity, and are numbered ... common allergens sources include animal products, drugs, foods, insect stings, fungal and pollens Animal products include fur, dander, wool and the dust mite (Dermatophagoides pteronyssinus and ... characterization and ligand binding studies of Der f will be presented as well These results may pave a way for understanding the allergenic properties of these proteins, and aid in the development of hypoallergens...

Ngày tải lên: 09/09/2015, 18:55