focus of the chapter1f
Synthesis of mannoside glycans of phosphatidylinositol mannosides (PIMs
Ngày tải lên :
10/09/2015, 15:50
... install the butenolide unit was not successful Chapter of the thesis focused on the synthesis of bielschowskysin based building blocks The target molecule was disconnected into two building blocks The ... making the pentamannoside 14 Part A: Synthesis of Mannoside Glycans of Phosphatidylinositol Mannosides (PIMs) 3.5 Synthesis of Mannoside Glycan Units of PIMs 3.5.1 Synthesis of Dimannoside of PIMs ... would facilitate future tagging of desired linkers for immunogenic studies of PIMs The major part of my research work focused on synthetic studies towards the synthesis of bielschowskysin macrocycles,...
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Báo cáo khoa học: New insights into the functions and N-glycan structures of factor X activator from Russell’s viper venom pot
Ngày tải lên :
07/03/2014, 06:20
... sequenced Of these, 10 clones were found to encode the LC2 and LC1 subunits Others were found to encode other variants of the C-lectin-like venom proteins The amino acid sequences of both subunits ... HC, the light chains appear to confer the substrate specificities of RVV-X [12] Collectively, the primary sequences of the three subunits of RVV-X (Figs and 5) suggest the possible presence of ... peptide of 23 residues, and the predicted sequence for its mature protein matches that published previously [8] The amino acid sequences of LC1 and LC2, together with those of other homologues of...
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Báo cáo khoa học: Total chemical synthesis and NMR characterization of the glycopeptide tx5a, a heavily post-translationally modified conotoxin, reveals that the glycan structure is a-D-Gal-(1fi3)-a-D-GalNAc pot
Ngày tải lên :
23/03/2014, 13:20
... with the calculated monoisotopic [M-2CO2-H]– mass of 1840.42 Da) The mass shift of Da (MR ) M) confirms the formation of the two disulfide bridges However, comparison of the retention times of the ... that even in the synthesis of nondisulfide bridge-containing glycopeptides the yield is dramatically affected by the scale of the reaction, the excess of amino acids used, and the level of purity ... did not further determine the configuration of this carbohydrate However, the different elution profiles of the native tx5a and the synthetic peptides constructed with the carbohydrate in the T-antigen...
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Báo cáo khoa học: N-Glycan structures of squid rhodopsin Existence of the a1–3 and a1–6 difucosylated innermost GlcNAc residue in a molluscan glycoprotein pot
Ngày tải lên :
23/03/2014, 17:22
... Table On the basis of the GU data of glycan A, i.e GU(ODS) of 8.8 and GU(amide) of 5.6, the reference compound 010.1F, which has been reported to exhibit GU(ODS) of 8.6 and GU(amide) of 5.5 [17], ... rhodopsins of other species Identification of the glycan B1 structure Fig Elution profile on the ODS column of the PA-oligosaccharide mixture obtained from squid rhodopsin The molecular mass of glycan ... as 4-linked Fuc on the basis of the EI-MS pattern (Fig 4B) Taken together, the results unambiguously establish that the extra b-Gal residue is 4-linked to the Fuc on the arm of a difucosylated...
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Báo cáo khoa học: Glycan profiling of urine, amniotic fluid and ascitic fluid from galactosialidosis patients reveals novel oligosaccharides with reducing end hexose and aldohexonic acid residues ppt
Ngày tải lên :
29/03/2014, 09:20
... moieties of glycosphingolipids On the basis of the presence of carbohydrate moieties of glycosphingolipids, we speculate about the potential involvement of a ceramide glycanase in the catabolism of ... below Two isomers of the glycan H2 were detected The retention time of the late-eluting H2 isomer was identical to that of maltose (Glc(a1–4)Glc; Table 2) The retention time of the early-eluting ... 3%; Table 3) The cause of C1 oxidation of the reducing end is unknown We can exclude the possibility that these species were observed due to oxidation of reducing sugars during the chromatographic...
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Báo cáo khoa học: Introduction of extended LEC14-type branching into core-fucosylated biantennary N-glycan Glycoengineering for enhanced cell binding and serum clearance of the neoglycoprotein pot
Ngày tải lên :
30/03/2014, 16:20
... presented on a cell surface by adsorbing the neoglycoprotein to the plastic surface of microtiter plate wells The homogeneity of the structure of the synthetic N-glycan, rigorously controlled by ... to the preparation of the other N-glycan-bearing probes tested previously [12–14] Solid-phase assay The matrix for the assay was established by adsorption of neoglycoprotein to the surface of ... and contributes to the complex changes in the glycoproteomic profile of the LEC14 mutant vs wild-type cells [18] The enzymatic introduction of the b1,2-linked GlcNAc moiety into the biantennary N-glycan...
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History Of Modern Biotechnology II
Ngày tải lên :
08/08/2012, 09:49
... element: the length of the main hypha (Le ), the total length of all the hyphae (L t ), the number of tips (n) and the length of a hyphal growth unit (Lhgu ) The Lhgu is defined as L t /n 3.2 The ... constant rate at the wall of the hyphae, transported to the tips and used for growth Under the influence of growth, the ratio of cytoplasmic volume and the number of nuclei at the tips in the apical ... on the field of genetic engineering The stabilization of the plasmid-carrying microorganisms had to be solved, as did the suppression of growth of the plasmid free host The natural folding of the...
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History of Modern Biotechnology I - Springer
Ngày tải lên :
08/08/2012, 09:52
... Production of the antibiotics is the primary factor in the competitive fitness of P aureofaciens in the rhizosphere and the relationships between it, the plant, and the fungal pathogens The antibiotic, ... University of Wisconsin isolated ultraviolet-induced mutants of Demerec’s strain One of these, Wis Q-176, which produced 550 mg/ml, is the parent of most of the strains used in industry today The further ... intermediates of the shikimic acid pathway, the tricarboxylic acid cycle, and from amino acids The regulation of the biosynthesis of secondary metabolites is similar to that of the primary processes,...
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09 Physical and Chemical Characteristics of DDGS revisions.
Ngày tải lên :
08/08/2012, 10:03
... 127 to 621º C The amount of time DDGS spends in the dryer also influences the color In general, the higher the dryer temperature and the longer DDGS remains in the dryer, the darker the resulting ... than 600 microns The weights of DDGS collected on each screen were then used to calculate the percentage of weight of each fraction of the total separated In addition to determining the average particle ... testing equipment is the primary equipment used to measure the strength and flow properties of bulk materials They also measure the amount of compaction as well as the bulk strength of materials (Rosentrater,...
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Use of DDGS in Swine Diets
Ngày tải lên :
08/08/2012, 10:03
... rates of 05 - Use of DDGS in Swine Diets 0, 15 and 30% without supplemental fat The second trial used the same dietary levels of DDGS as in the first trial, but also evaluated the effect of adding ... decrease the quality and acceptability of U.S pork in the Japanese export market 05 - Use of DDGS in Swine Diets Gralapp et al (2002) conducted two experiments to evaluate the impact of the level of ... supplemental fat was added, the iodine values of carcass fat from pigs fed high concentrations of DDGS would be lower and the negative effects of adding high levels of DDGS to the diets on pork fat...
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