... Madin–Darby canine kidney (MDCK) cells, a study of chimeric forms of DPP IV has shown that the luminal domain of DPP IV carries dominant apical sorting information while the short cytoplasmic tail ... representation of the three major domains (sinusoidal, lateral, bile canalicular) of the hepatocytes present in the PM fraction [50] To the best of our knowledge, tyrosine phosphorylation of DPP IV ... targeting is beginning to be perceived For instance, one striking example of c-Src location inside one organelle has been reported for the inner membrane of mitochondria of osteoclasts [57] Moreover,...
Ngày tải lên: 19/02/2014, 07:20
... of calcium is important for the optimal activity of BsArb43B Furthermore, to determine whether the role of the calcium is structural, thermal shift assays were performed to determine the Tm of ... different colours: blue for BsArb43B, magenta for BsArb43A, green for ABNTS, dark grey for AbnB, red for exo-Abn towards the solvent region, and presumably the other residues of the arabinohexose ... characteristic of this type of enzymes Superposition of the Ca trace of the BsArb43B FEBS Journal 277 (2010) 4562–4574 ª 2010 The Authors Journal compilation ª 2010 FEBS 4563 Role of calcium in...
Ngày tải lên: 15/03/2014, 23:20
Báo cáo khoa học: Evidence for the presence of ferritin in plant mitochondria pdf
... boiled at 95 °C for The sample for electrophoresis analysis was obtained by collecting the supernatant ( 30 lL) after centrifugation at 12 000 g for 20 s and addition of 10 lL of 75% (w/v) glycerol ... suggested, they represent ferritin and a product of degradation of the same protein [14] The same crossreactivity was also detected for a protein of twofold purified pea stem mitochondria (result not ... presequences of the chloroplastic protein transit peptide [28], similarly to what reported for pea ferritin [29,30] On the other hand, the analysis of the presequence of AtFer4 reveals a high score for...
Ngày tải lên: 16/03/2014, 18:20
Báo cáo khoa học: Re-evaluation of intramolecular long-range electron transfer between tyrosine and tryptophan in lysozymes Evidence for the participation of other residues doc
... yields of fragmentation (Table 2) vary for these proteins Discussion In this work, we performed a comparative study of the oneelectron oxidation of six proteins from the c-type family of lysozymes; ... hydrolysis of irradiated solution, for which the amino acid composition of peptides was determined, were screened for the fluorescence signal obtained when an excitation wavelength of 380 nm is ... achieved by calculation of the portion of the total area under the curve occupied by the dimers peak The obtained value was divided by two MALDI-TOF MS was performed at The Institute of Nuclear Physics,...
Ngày tải lên: 17/03/2014, 10:20
Báo cáo Y học: Evidence for general stabilization of mRNAs in response to UV light pdf
... staining of 28S rRNA is shown to allow comparison of RNA amounts loaded (C) Quantification of results for BBB-IL-8972)1310 mRNA from an experiment performed as in (B), but with different doses of UV ... before the assay of RNA stability Half-lives for BBB-IL-8972)1310 were quantified as described in Materials and Methods 5834 F Bollig et al (Eur J Biochem 269) Ó FEBS 2002 1) Formation of two of ... consisting of the ARE of GM-CSF or the AU-rich region of the IL-8 3¢-UTR (nucleotides 972–1310) Where indicated, antibodies specific for HuR (0.75 lg), AUF1 (1 lL of a : 10 dilution of serum) or...
Ngày tải lên: 31/03/2014, 08:20
martin - the organisation of conceptual knowledge in the brain (psychology press, 2003)
... T.W.James, K.M.Curby, and M.J.Tarr 352 Role of mental imagery in a property verification task: fMRI evidence for perceptual representations of conceptual knowledge I.P.Kan, L.W.Barsalou, K.O.Solomon, ... J K., & Thompson-Schill, S.L (2003) Role of mental imagery in a property verification task: fMRI evidence for perceptual representations of conceptual knowledge Cognitive Neuropsychology,20, 525–540 ... type of C-S deficit and type of conceptual knowledge deficit In fact, the authors show that knowledge of both sensory and functional information is equally impaired in the overwhelming majority of...
Ngày tải lên: 03/04/2014, 12:07
ancient israel in sinai the evidence for the authenticity of the wilderness tradition oct 2005
... results of wider study of the giving of law and covenant in Sinai, of the results of modern study of the ancient use of portable structures (‘‘tabernacles’’) for worship and cult, and of the occurrence ... prior permission of Oxford University Press Library of Congress Cataloging-in-Publication Data Hoffmeier, James Karl, 1951– Ancient Israel in Sinai : the evidence for the authenticity of the wilderness ... Journal of Near Eastern Studies Jewish Publication Society Jewish Quarterly Review Journal for the Study of the Old Testament Journal of Semitic Studies Journal of the Society for the Study of Egyptian...
Ngày tải lên: 11/06/2014, 01:36
Báo cáo sinh học: "Phylogenetic evidence for the distinction of Saaremaa and Dobrava hantaviruses" pot
... TreePuzzle for a smaller data set Phylogenetic tree created with TreePuzzle for a smaller data set The tree is based on the nt 37–1232 of the S segment sequences Page of (page number not for citation ... likelihood method for reconstructing tree topologies Mol Biol Evol 1996, 13:964-969 Posada D, Crandall KA: MODELTEST: testing the model of DNA substitution Bioninformatics 1998, 14:817-818 Swofford DL: ... outgroup In the case of maximum likelihood trees, the use of additional hantavirus sequences as outgroup resulted in a lower bootstrap support for SAAV In fact, a 100% support for SAAV monophyly...
Ngày tải lên: 19/06/2014, 08:20
báo cáo hóa học:"Phylogenetic evidence for the distinction of Saaremaa and Dobrava hantaviruses" pdf
... TreePuzzle for a smaller data set Phylogenetic tree created with TreePuzzle for a smaller data set The tree is based on the nt 37–1232 of the S segment sequences Page of (page number not for citation ... likelihood method for reconstructing tree topologies Mol Biol Evol 1996, 13:964-969 Posada D, Crandall KA: MODELTEST: testing the model of DNA substitution Bioninformatics 1998, 14:817-818 Swofford DL: ... outgroup In the case of maximum likelihood trees, the use of additional hantavirus sequences as outgroup resulted in a lower bootstrap support for SAAV In fact, a 100% support for SAAV monophyly...
Ngày tải lên: 20/06/2014, 04:20
báo cáo hóa học:" A decade of modelling research yields considerable evidence for the importance of concurrency: a response to Sawers and Stillwaggon" potx
... stage of infection These empirical estimates for coital frequency were then used in Goodreau et al [27] for the three of their four transmission models that were built off of published estimates for ... knowledge of acute infection strengthen the modelling evidence in favour of the concurrency hypothesis The early work on concurrency did not include different levels of infectivity by stage of ... biological data in all of the ways that Sawers and Stillwaggon outline as necessary for a realistic model, although all incorporated some forms of data Nevertheless, this line of work confirmed the...
Ngày tải lên: 20/06/2014, 08:20
Báo cáo sinh học: "Evidence for large domains of similarly expressed genes in the Drosophila genome" docx
... pattern of gene expression that mirrors this Journal of Biology 2002, 1:5 http://jbiol.com/content/1/1/5 Journal of Biology 2002, Table The number of groups of genes, and total numbers of genes ... so we used a window size of ten for the remainder of our analysis There are no qualitative differences in the nature of groups identified by larger window sizes Many of the ten-gene groups that ... expression was calculated for genes in a sliding window across the genome, and this process was repeated for windows of different sizes The net number of genes (that is, the number of genes in groups...
Ngày tải lên: 06/08/2014, 18:20
Báo cáo lâm nghiệp: "Whole tree hydraulic conductance and water loss regulation in Quercus during drought: evidence for stomatal control of embolism?" doc
... occurred in the leaf xylem of the drought trees in the absence of regulation We can thus predict that because of these tensions and because of runaway embolism, the degree of embolism would have ... revealed the role of specific hormones (ABA) in the control of g during drought Roots in s the dry zones of the soil are producing ABA that provoke stomatal closure An argument in favor of hormonal ... computed that for the driest trees, the dif- ference between dF and dF cavitation midday represented less that a few percent of the midday dF of the control trees It can be noted that for some droughted...
Ngày tải lên: 08/08/2014, 18:21
báo cáo khoa học: "Structure and expression of the maize (Zea mays L.) SUN-domain protein gene family: evidence for the existence of two divergent classes of SUN proteins in plants" pot
... the nucleoplasm and therefore allow forces produced within the cytoplasm to be transmitted to the nuclear periphery Evidence for the expression and NE localization of Page of 22 plant SUN-domain ... first description of relative mRNA expression levels of all members of a SUN gene family in any plant species and may therefore prove useful to investigators of the functions of plant SUN-domain ... typical of the entire class of PM3 proteins Localization of a Maize PM3-type Protein To test for the presence and localization of ZmSUN3/4 proteins in planta, we developed peptide antibodies for...
Ngày tải lên: 11/08/2014, 11:21
báo cáo khoa học: " Multiple evidence for the role of an Ovate-like gene in determining fruit shape in pepper" pptx
... 50°C for min, 95°C for min, followed by 35 cycles of incubation at 95°C for 20 s, 58°C for 20 s, 72°C for 20 s, and a final extension step of 10 at 72°C In order to identify possible effects of ... organization of the gene The cycling parameters were: 50°C for min, 95°C for min, followed by 35 cycles of incubation at 95°C for 20 s, 58°C for 20 s, 72°C for 25 s, and a final extension step of 10 ... parameters were: incubation at 50°C for min, 95°C for min, followed by 35 cycles of incubation at 95°C for 20 s, 58°C for 20 s, 72°C for 20 s, and a final extension step of 10 at 72°C To identify the...
Ngày tải lên: 11/08/2014, 11:21
báo cáo khoa học: " Molecular, genetic and transcriptional evidence for a role of VvAGL11 in stenospermocarpic seedlessness in grapevine" pptx
... current knowledge of the possible functions of the genes in the region confirmed the former hypothesis of Costantini et al [4] that VvAGL11 is the best positional candidate gene for the control of ... positional evidence for VvAGL11 as being the major gene responsible for seedlessness [4] Here, we provide genetic and transcriptional support for this hypothesis and discuss its potential for molecular-assisted ... gene for seedlessness, and we provide experimental evidence suggesting that the seedless phenotype might be caused by variations in its promoter region Current knowledge of the function of its...
Ngày tải lên: 11/08/2014, 11:21
Báo cáo y học: " Evidence for genetic association of RORB with bipolar disorder" pptx
... irritable only) of seven symptoms during the period of mood disturbance Also recorded was the onset of first episode, the number of episodes, offset of last episode, and total duration of illness ... subcommittee for human subjects of our institution All subjects' parents or guardians signed written informed consent forms and children older than years of age signed written assent forms Potential ... for multiple testing was implemented based on the number of SNPs analyzed per gene The critical P-value for a positive association was therefore 1.6 × 10-4 for SNPs from RORA and 1.2 × 10-3 for...
Ngày tải lên: 11/08/2014, 17:20
báo cáo khoa học: " A review of the evidence for the effectiveness of primary prevention interventions for Hepatitis C among injecting drug users" potx
... definition of anti-HCV positivity (type of biochemical test used); location (city, country, number and type of treatment settings); years of recruitment (and total duration of recruitment); number of ... [http://www.biomedcentral.com/content/supplementary/14777517-3-27-S1.doc] Acknowledgements This is an edited version of the World Health Organization Health Evidence Network synthesis of the evidence base pertaining to effectiveness of interventions ... reported in the form of a narrative systematic review Such a narrative format has been described as appropriate in reporting the results of observational studies identified through a process of systematic...
Ngày tải lên: 11/08/2014, 20:20
Báo cáo y học: " Angiotensin-converting enzyme 2 autoantibodies: further evidence for a role of the renin– angiotensin system in inflammation" potx
... findings are of potential importance in our understanding of the role of circulating and tissue sources of ACE2, particularly in various disease states Increased circulating levels of ACE2 may ... administration of recombinant soluble ACE2, as recently demonstrated in models of diabetic nephropathy [8] and liver fibrosis [9] or in the genetic expression of ACE2 in pulmonary hypertension [10] Page of ... study of the renin–angiotensin system has now surpassed the century mark, the characterization of this system and identification of the factors that regulate the expression or activity of its...
Ngày tải lên: 12/08/2014, 14:22
Báo cáo y học: "Genome-wide analysis of primary CD4+ and CD8+ T cell transcriptomes shows evidence for a network of enriched pathways associated with HIV disease" pot
... set enrichment analysis: A knowledge- based approach for interpreting genome-wide expression profiles Proceedings of the National Academy of Sciences of the United States of America 2005, 102:15545-15550 ... GOTree In GOTree, O stands for observed gene number in the category; E for expected gene number in the category; R for ratio of enrichment for the category; and P for p value calculated from ... number in the category; E for expected gene number in the category; R for ratio of enrichment for the category; and P for p value calculated from the statistical test given for the categories with...
Ngày tải lên: 13/08/2014, 01:20
Báo cáo y học: " Evidence for preferential copackaging of Moloney murine leukemia virus genomic RNAs transcribed in the same chromosomal site" ppt
... the main reason for the preferential formation of homodiploid genomes in Mo-MuLV In spite of substantial differences in the methods, the estimations of the efficiency of homodimer formation were ... populations of RNA molecules left in a monomeric form and/or unstable homodimers The diploidy of retroviral genome supposes that two molecules of RNA could be necessary for replication of virus ... RT-PCR4analysis of virion RNAs RT-PCR analysis of virion RNAs A Plasmid structures of retroviral leader regions L1 and L2, primers used for PCR amplification; sizes of DNA fragments and positions of EcoRI...
Ngày tải lên: 13/08/2014, 13:20