fluorescence excitation for one and two photon microscopy

Ngày tải lên: 05/08/2014, 15:20

... chemotherapy in group II, three patients (one patient after first cycle and two after two cycles) developed gastric perforation and two patients (after first cycle) gastrointestinal bleeding Both of ... incidence of severe haemorrhage or perforation, accounting for 2.1% and 1.7%, respectively, of those individuals treated with chemotherapy alone, and 2.2% and 0.9%, respectively, of surgically-treated ... the final manuscript YB and HK: conceived of the study, and participated in its design and coordination HE and MI: review of the final manuscript and revising it critically for important intellectual...

Ngày tải lên: 10/08/2014, 22:21

... Criterion validity of the Short Mood and Feelings Questionnaire and one- and two- item depression screens in young adolescents Child and Adolescent Psychiatry and Mental Health 2010 4:8 Submit your ... child and parent using the computerized version of the DISC, version four [29] The parent and child MFQ and the parent and child DISC were administered within a single two- to-three hour period For ... without for all reporter versions (p < 001 for all comparisons) The SMFQ showed high internal reliability for both the child and parent version (a = 84 for both) The correlation between the SMFQ-C and...

Ngày tải lên: 13/08/2014, 18:21

... injected and controlled in epitaxial graphene via quantum interference between photocurrents generated by one- photon and two- photon interband transitions In order to explore the two- photon absorption ... is up to about one millimeter The multi -photon excitation microscopy uses red-shifted excitation light, which can also excite fluorescent dyes However, for each excitation, two photons of the ... used in multi -photon excitation microscopy It allows the high photon density and flux required for multi -photon absorption and is tunable across a wide range of wavelengths Multi -photon absorption...

Ngày tải lên: 09/09/2015, 17:55

... A two dimensional PDE for the options is derived We also take into account of transaction costs and position limits and form complete models For numerical experiement, we use fully implicit and ... stock index futures and plotting their early exercise boundaries We consider both one dimensional and two dimensional problems, for each we sub-divide as ‘no position limits’ case and ‘with position ... commissions + one market-impact cost  C = one market-impact cost 1.2 Historical Work And Author’s Contribution 1.2 Historical Work And Author’s Contribution Numerous famous academicians and practitioners...

Ngày tải lên: 30/09/2015, 14:16

... categories, one of which uses fixed grid for both fluid and the solid solver (Euler-Euler), another one uses fixed grid for fluid and moving grid for the solid (Euler-Lagrangian) and the other one uses ... gas-solid and water-solid compressible flow and formulated the conditions for the possible shock wave refraction patterns He applied the MGFM and the simplified MGFM for the one dimensional flow and ... Cheong and Dr Liu Tiegang for introducing me to the fascinating and challenging field of Fluid Structure Interaction I would like to thank them for their invaluable guidance and support and encouragement...

Ngày tải lên: 27/11/2015, 11:12

... nonlinear microscopy of highly scattering biological tissue As a nonlinear measurement technique our method shares the well known advantages of two- photon fluorescence microscopy over linear microscopy: ... Tian and W S Warren, “Ultrafast measurement of two- photon absorption by loss modulation,” Opt Lett 27, 1634–1636 (2002) 22 W Liu, O Kosareva, I S Golubtsov, A Iwasaki, A Becker, V P Kandidov, and ... caused a TPA signature and vice versa To confirm this finding we performed numerical simulations for the case of pure SPM and pure TPA media The results are displayed in Fig 5(a) and are in qualitative...

Ngày tải lên: 21/12/2016, 10:57

... experiments and analysis of the data, PD, JPC and ES set up the platform for FCS and FLIM, CC produced eGFP for in vitro controls, DM gave plasmid and expertise for cellular studies, SB and NM performed ... the formation of Vpr oligomers and their intracellular localization, we used eGFP and mCherry Vpr fusion proteins and studied their interaction by two photon fluorescence lifetime imaging microscopy ... showing the two putative hydrophobic platforms for Vpr oligomerization The two platforms available for Vpr oligomerization, in the first and third helices, have been colored in red and hydrophobic...

Ngày tải lên: 13/08/2014, 05:21

... excitation fluorescence microscopy 13 II Biological applications of two- photon excitation fluorescence microscopy 15 E Principle of two- photon excitation fluorescence microscopy ... Figure 12 Two- photon laser scanning microscope 75 Figure 13 Heart perfusion chamber used for TPLSM imaging 76 Figure 14 Two- photon excitation- induced tissue autofluorescence and EGFP fluorescence ... excitation fluorescence microscopy Principle of two- photon excitation fluorescence microscopy Standard epifluorescent microscopy is excellent for obtaining fluorescence from thin sections of tissue...

Ngày tải lên: 24/08/2014, 12:57

... 97 5.4 Two- photon transition probability for rubidium 99 Experimental setup for rubidium two- photon spectroscopy and results 104 6.1 Two- photon laser ... The basics of the Two- photon transition 93 5.1 Doppler-free two- photon spectroscopy 95 5.2 Two- photon absorption lineshape 95 5.3 Two- photon transition ... parallel to the surface of the chip and bring it near to a microoptics for detection and manipulation using a two- photon transition A two- photon transition scheme for the Rubidium (Rb) 5S1/2 to 4D5/2...

Ngày tải lên: 08/09/2015, 15:07

... defects and performs favourably in two stage revisions of THR 29 However, unvascular- 249 ized bone grafts are at risk to become contaminated and need protection as well When loading bone grafts ... vancomycin-intermediate and vancomy- of large amounts of gentamicin sulphate into acrylic bone cement: effect on cin-resistant Chemother handling and mechanical properties, antibiotic release, and biofilm formation ... concentrations for elimination18 and such usually are inaccessible for systemic antibiotic therapy as well as for antibiotics released from PMMA 19,20 Debridement Radical debridement is prerequisite for...

Ngày tải lên: 26/10/2012, 09:53

... to provide two- dimensional (2-D) cursor control from EEG Wolpaw et al measured band power from 64 channels, from both hemispheres and two different bands simultaneously, with each band controlling ... becomes visible, and the cursor moves to the new position The entire process for one (two- bit) cursor move takes about 15 s When the game is played without hand movements (as in one of our supplementary ... only one direction, the control is two- dimensional rather than one- dimensional because the direction of each movement can be any one of four choices in two dimensions This is analogous to the two- dimensional...

Ngày tải lên: 19/06/2014, 08:20

... each block, where (a) is for direct transmission, (b) is for one- way relaying, and (c) is for two- way relaying Figure Energy efficiency comparison with zero circuit power and symmetric bidirectional ... such as DF and compress -and- forward (CF) that provide higher rate regions than AF However, AF is also widely considered in practice [6], and is superior to DF in outage performance for TWRT when ... form expressions for the optimal transmission time and the optimal SEs, there are also no closed form expressions for the optimal EEs We will use simulations to compare the EEs of DT, OWRT, and...

Ngày tải lên: 21/06/2014, 17:20

... the clot components in two Z-stacks, one for a typical wild-type injury and the other for a typical FVII deficient type injury (here, (+, +, −), e.g., means the red channel value and green channel ... changes in time (red for platelets, green for fibrinogen/fibrin, and black for other blood cells) pare biological experimental thrombi monitored by multiphoton microscopy for their development ... multiphoton microscopy in a single optical plane In addition to the confocal video microscopy in one plane, we can also generate a vertical stack of 2 -photon images that can be compiled to form...

Ngày tải lên: 21/06/2014, 19:20

... studied 1.5 and its variants in a Hilbert space H or in a Banach space X; see, for example, 13, 19–23 and the references therein Let X be a uniformly smooth and uniformly convex Banach space and let ... introduce and study two new iterative algorithms for finding a common element of the set EP of solutions for the generalized equilibrium problem 1.2 and the set T −1 ∩ T −1 for maximal monotone operators ... relatively nonexpansive if F S F S and φ p, Sx ≤ φ p, x , for all x ∈ C and p ∈ F S 15 Observe that, if X is a reflexive strictly convex and smooth Banach space, then for any x, y ∈ X, φ x, y if and...

Ngày tải lên: 22/06/2014, 02:20

... [16-18] Single-molecule forces Two principal methods are available for resolving the forces exerted by single molecules [19], optical traps (or tweezers) and atomic force microscopy (AFM), both ... and excitation using evanescent-wave illumination - that is, total internal reflection fluorescence (TIRF) microscopy - permit the simultaneous detection of single-molecule fluorescence and force ... polymer [27] Combining fluorescence and force measurement In contrast to the above examples, there have been relatively few experiments that measure single-molecule forces and fluorescence at the...

Ngày tải lên: 06/08/2014, 18:20

... in C assimilation alter 1) the import of maternal C and N, 2) N uptake and assimilation, 3) partitioning of C and N between shoot and roots and 4) the time lapse prior to a complete independence ... contained the canopy of the plants, and the lower one (250 L) the soil containers The two parts were separated by an opaque plastic cover with 20 holes (one for each container) Access to the inside ... weighed and ground to a fine , homogeneous powder with a laboratory mill Samples were stored at -20 °C before analysis of biochemical content and isotope composition Total C and N contents and isotope...

Ngày tải lên: 08/08/2014, 14:21

... polymerase for 10 at 95°C and 40 cycles of two PCR steps, 95°C for 15 sec and 60°C for 60 sec One- step qRT-PCR was used for analysis of env transcript levels in peripheral tissues of G45 and F9 chickens ... μl of the reaction mixture and then incubated for 10 at 50°C for cDNA synthesis, for at 95°C for RTase inactivation and 35 cycles of two steps with 10 sec at 95°C and 30 sec at 60°C to amplify ... 95°C and 40 cycles of two PCR steps, for 15 sec at 95°C and for 60 sec at 60°C Primers for chicken pro-opiomelanocortin (POMC, GeneBank accession NM_001031098) and pre-melanin-concentrating hormone...

Ngày tải lên: 12/08/2014, 23:21

... WA and Bitplane, Zurich, Switzerland), and it has been proposed as a standard recommendation for image data migration by the European Advanced Microscopy Network [20] Immediate applications for ... a relational database for information storage, and an XML-based file format used for transmission of this information and storage outside of databases The OME XML file format allows the exchange ... the OME XML file format is not raw performance, but interoperability above all else, using widely accepted standards and practices for information exchange As the OME XML file format has gained...

Ngày tải lên: 14/08/2014, 14:21

... collect subfemtoliter focal volumes [11] Because Figure Fluorescence excitation for one- and two- photon microscopy In one- photon fluorescence microscopy, a continuous wave ultraviolet or visible ... diagram Jablonski diagram demonstrating one- and two- photon fluorescence excitation Two- photon excitation results from the simultaneous absorption of two low-energy photons by a fluorophore state Because ... A Multiphoton fluorescence excitation microscopy in biomedical research .1 B Multiphoton fluorescence excitation microscopy Multiphoton fluorescence excitation Lasers...

Ngày tải lên: 24/08/2014, 13:39