flow online monitoring of cell responses

... ONLINE MONITORING OF NITROGEN GREENHOUSE GASES FROM WATER RECLAMATION PLANTS WANG MENG (B.Eng (Hons.), National University of Singapore) A THESIS SUBMITTEDFOR THE DEGREE OF MASTER OF ENGINEERING ... Table 3.1 Daily monitoring frequency of online monitoring system 36 Table4.1 Average exhaust gas emission rate into SEIFC and corresponding advective flux calculated of aerobic zones of each basin ... Sketch of BNR bioreactor (Train 2) in CWRP 27 Figure 3.3 Sketch of one basin of BNR reactor in SW of UPWRP 30 Figure 3.4 Relationship between real gas flow rate and calculated gas flow...

Ngày tải lên: 27/11/2015, 12:12

... the number of cells used in each sample does not affect the readout of the ICS Since the procedure of manual cell counting is a usual source of experimental error and the number of cells directly ... ,)1 0,3 Figure Number 4of detected positive responses Number of detected positive responses (A) The histogram plots show the number of positive CD8, CD4 or total T -cell responses detected with ... P, Byl B, De Groote D: Monitoring of cellular responses after vaccination against tetanus toxoid: comparison of the measurement of IFN-gamma production by ELISA, ELISPOT, flow cytometry and real-time...

Ngày tải lên: 10/08/2014, 05:21

... the cells is necessary for the maintenance of oscillations Removal of external Ca2+ leads to a cessation of oscillations in most cases in endodermal cells [62] and HeLa cells [63] In other cell ... General scheme of the main processes involved in intracellular calcium oscillations Meaning of the symbols for reaction rates: vb,j, net rate of binding of Ca2+ to the j-th class of Ca2+ buffer ... uptake into mitochondria; vmo, release of Ca2+ from mitochondria; vout, transport of Ca2+ out of the cell by plasma membrane Ca2+ ATPase; vplc, formation of IP3 and DAG catalyzed by phospholipase...

Ngày tải lên: 24/03/2014, 00:21

... and 31% of these cell internet users go online MOSTLY using their cell phone instead of some other device (Example of how to read this chart: 45% of cell internet users ages 18-29 go online mostly ... group % of cell internet users who… Go online mostly on cell phone Use internet on cell phone, but go online mostly using other device Use both equally / It depends Don’t go online using cell phone ... smartphone of some kind, and 90% of these smartphone owners say that they use their phone to go online. 2 More than half of adult cell owners go online using their phones % of adult cell owners...

Ngày tải lên: 29/03/2014, 20:20

... perturbing a variety of other cellular processes, including cell cycle regulation [46,47], apoptosis [48], and cell architecture The IE1 protein of HCMV is a major source of CD8 T -cell epitopes for ... HLA-class I (W6/32) of antibodies, at a concentration of 25 μg/mL, were pre-incubated with the target cells for 30 minutes before addition of the stimulated T-cells K562 cells were used as targets ... use of GM-CSF and IL-4 during adher- Figure Immunofluorescence on HEK293 cells Immunofluorescence on HEK293 cells Microphotographs show fluorescent labeling for AAV/IE1 (A, B) in HEK293 cells...

Ngày tải lên: 18/06/2014, 15:20

... of CD8 T -cell populations magnetic beads Flow and without depletion of CD8 cells byin PBMC samples Flow cytometry of CD8 T -cell populations in PBMC samples with and without depletion of CD8 cells ... allow identification of the type and specificity of the T -cell responses that hold particular importance for control of the disease A full understanding of the cellular immune responses in humans ... small numbers of antigens, whereas the CD4 responses recognize a broad range of antigens CD8 responses Definition of CD8 responses Eight of the 17 infected subjects had positive CD8 responses with...

Ngày tải lên: 20/06/2014, 01:20

... on the surface of activated T cells with a sensitivity of less than 200 molecules per cell, it can be shown that expression is independent of the proliferative history of the cell and is exclusively ... CTLA-4-expressing cells represent a distinct pool of memory T cells [11,15] Control of apoptosis by CD28 and CTLA-4 The decision between the survival and apoptosis of T cells is of particular importance ... CTLA-4–/– Treg cells are able to downregulate the activation of target cells; on the other, blockade of CTLA-4 abrogates the inhibitory function of Treg cells [72] Interestingly, naive T cells, converted...

Ngày tải lên: 09/08/2014, 01:23

... evidence of relapse Similarly, 10 of our patients had only once instance of elevated serum SCC-Ag, with normalization at subsequent follow-ups None of these 10 patients had any evidence of disease ... Goyang-si, Gyeonggi-do, 410-769, Republic of Korea Department of Radiation Oncology, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Republic of Korea Yoon et al Radiation Oncology ... antigen of human cervical squamous cell carcinoma Cancer 1977, 40:1621-1628 Maruo T, Shibata K, Kimura A, Hoshina M, Mochizuki M: Tumor-associated antigen, TA-4, in the monitoring of the effects of...

Ngày tải lên: 09/08/2014, 09:20

... number of daughter cells according to the ratio of the final cell volume to the initial cell volume Acknowledgements We thank Prof R Kamiya of Univ of Tokyo and Prof S Ishiwata of Waseda University ... in excess of that required for the maintenance of cell metabolism is needed for growth The volume of Chlamydomonas cells and the growth rate dependence of the cell volume changed in a nonlinear ... The interdivision time of cells illuminated continuously (LL) was dependent on the cell growth rate and on the reaching of a critical cell size The product of the rate of cell volume increase and...

Ngày tải lên: 11/08/2014, 00:22

... cell count before ART cells/µl CD4 T cell count at presentation of IRIS cells/µl Fold change in CD4 T cell counts from baseline to IRIS presentation CD4 T cell count after remission of IRIS cells/µl ... CD4 T -cell count of 22 cells/µl (interquartile range (IQR) 6.3–50.3) before initiation of ART, rising to 70 cells/µl (IQR 63–123) after http://www.jibtherapies.com/content/3/1/7 months of therapy ... initiation of immunotherapy there was a decrease in IL-4 production, which was paralleled by restoration of proliferative specific-anti-PPD T -cell responses Flow cytometry revealed higher levels of CD38...

Ngày tải lên: 11/08/2014, 10:23

... and CD8+ HIV-1specific T -cell responses was noted in 11 of 12 individuals To address the specificity of the responses generated by rhGH, we also assessed the CD4+ HTL responses to some other viral ... CD8+ T -cell responses were observed at baseline, in the absence of HIV-1-specific CD4+ T -cell responses, in one patient only Generally, daily administration of rhGH, in the presence of HAART, induced ... T -cell responses may be maintained for a limited period without CD4+ T -cell help [42], but may eventually decline following withdrawal of rhGH therapy The absence of virus-specific CD4+ T-cell...

Ngày tải lên: 11/08/2014, 10:23

... for a subset of HIV-specific CD8+ T cell responses escaped by the virus, the emergence of variant-specific T cell responses over time may allow for a degree of continued control of viral replication ... of virus-specific CD8+ T cells may also contribute to impairment of T cell control of HIV replication during early infection) The tremendous capacity of the virus to escape from CD8+ T cell responses ... 50 IFN-g spot-forming cells per million PBMC were considered positive Measurement of the functional avidity of CD8+ T cell responses The functional avidity of CD8+ T cell responses at sequential...

Ngày tải lên: 13/08/2014, 01:20

... activated CD4+ T cells Results are the average of triplicate samples of p24 supernatant of activated CD4+ T cells infected with each virus P-values shown above each time point compare the p24 of ES8-17 ... staining to elucidate the population of CD8 + T cells responding to peptide We found that the vast majority of IFN-g producing cells were also CFSElow, suggesting that cells producing cytokines in response ... recognized by CTL, IFN-g is not a good measure of an effective CTL response and does not correlate with control of viral replication [20-22] The capacity of CD8+T cells to proliferate in response to HIV-1...

Ngày tải lên: 13/08/2014, 01:21

... To examine the effect of in vivo IgE blockade on T cell responses, we first examined T cell proliferation using CFSE dye dilution by determining the percentage of CFSElow cells Minimal spontaneous ... frequency of Ag specific T cells is a major determinant of the magnitude of the proliferative response We thus hypothesized that blocking IgE in vivo would decrease the frequency of Ag specific T cells ... the identification of clonally expanded allergen specific T cells, the cytokine profile of which can be assessed by restimulation in vitro [15] Food allergen specific T cell responses in EG demonstrated...

Ngày tải lên: 13/08/2014, 13:22

... 16 Figure I-7: Flow chart of CHAPTER II 17 Figure I-8: Flow chart of CHAPTER III 18 Figure I-9: Flow chart of CHAPTER IV 19 Figure I-10: Flow chart of CHAPTER V ... Comparison of the current μc [cP] with a previous study at 21°C 83 x LIST OF FIGURES Figure I-1: Electron microscopic image of blood cell components Figure I-2: Typical example of arteriolar flow ... study on the suspension of blood, which includes blood rheology, cell mechanics and physiology Blood, a vital fluid of humans, is a concentrated suspension of cells and cell fragments in plasma...

Ngày tải lên: 09/09/2015, 10:14

... of cell surface markers for flow cytometry 54   2.5.2   Intracellular staining for flow cytometry 55   2.5.3   Sorting of naïve CD8+ T cells by flow cytometry 56   2.5.4   Sorting of ... Rates of proliferation of CD8+ T cells in the absence of IFN-γ signaling 140 5.4 Interferon gamma and cell death 146   viii 5.4.1   Rate of survival of CD8+ T cells in the absence of ... contraction of antigen-specific CD8+ T cell responses The increased accumulation of the effector CD8+ T cell population was independent of viral load and rates of proliferation of the cells Direct...

Ngày tải lên: 10/09/2015, 09:25

... basis of evidence he has In (27), there is a clash of non-observance of the maxim of Quantity and Quality A great number of similar cases are available in Vietnamese These patterns of responses ... patterns of responses to questions defined linguistic researchers in English and Vietnamese Lakoff (1973) mentions two kinds of responses to questions: answers and replies According to Lakoff, a ... types of question responses from the set of English Native Speakers in English Second, the Vietnamese translated version of the DCT questionnaire was used to collect some types of question responses...

Ngày tải lên: 07/11/2012, 14:54

... the balance of expression of members of the Myc/Max/Mad network of proteins (see Fig 1) may dictate the response to loss of Myc (Walker et al 2005) In mice, the effects of deletion of the c-myc ... excision of c-myc (de Alboran et al 2001) Importantly, deletion of the mnt gene, which encodes a member of the Mad family of antagonists of Myc, restores proliferation to c-myc deleted cells, suggesting ... neuronal precursor cells leads to a dramatic loss of proliferative capacity of such cells (Knoepfler et al 2002) As long as questions of redundancy between different members of the myc gene family...

Ngày tải lên: 25/10/2013, 21:20

... in Mouse Models of Cell Cycle Regulators One of the hallmarks of cancer cells is misregulation of cell proliferation Cancer cells are able to evade the normal signals that stop cell division and ... the mechanisms of regulation of normal cell proliferation sets the ground for understanding the deregulated proliferation characteristic of a cancer cell regardless of the type of cancer or where/how ... within certain cell types such as trophoblast giant cells of the placenta and megakaryocytes, but it can also be observed in cells due to a defect in the cell cycle regulation of these cells For example...

Ngày tải lên: 25/10/2013, 21:20