five decades of investigation of somitogenesis in xenopus laevis

... expression is obtained as a consequence of inserting the cDNAs between the 5¢- and 3¢-UTR of b-globin, a protein that is highly expressed in Xenopus laevis oocytes GST-fusion proteins of XPR130, XN73 ... phosphoimager-intensifying screen Results cDNA cloning of PR72/130/B¢¢ subunits from Xenopus laevis prophase oocytes To clone the PR72/130/B¢¢ subunits of PP2A from Xenopus laevis, a prophase Xenopus ... cell cycle was initially suggested through experiments using okadaic acid (OA), an inhibitor of PP1 and PP2A Induction of meiotic oocyte maturation occurred after injection of OA into Xenopus [16]...

Ngày tải lên: 08/03/2014, 08:20

... membrane voltage which, in turn, results in the opening of inward-rectifying K+ channels, allowing the in ux of K+ ions [4] For stomatal closure, increased levels of cytosolic Ca2+ inhibit plasma membrane ... In an in vitro phosphorylation assay, the Vicia AAPK ⁄ ABR kinase has been shown to phosphorylate the C-terminal region of KAT1 [16] One of the 10 members of the SNF1-related protein kinase in ... T308 in KAT1 Modification of T306 abolished KAT1 activity in oocyte recordings, whereas modification of T308 did not cause a loss of function [21] In animal cells, one type of Ser ⁄ Thr protein kinase,...

Ngày tải lên: 22/03/2014, 21:21

... family of RNA binding proteins [31–33] Together with the findings obtained in the present study, interaction of VICKZ and poly(A) binding proteins thus appears to define a conserved feature of mRNPs ... presence of 42Sp50 in nucleus and cytoplasm indicates that it might be a shuttling protein with a function in the nuclear export of localizing mRNAs To determine whether 42Sp50 is capable of shuttling, ... only (TAP) was included Proteins contained in these preparations were separated by SDS ⁄ PAGE and visualized by colloidal Coomassie staining (Fig 1A); the arrays of proteins interacting with either...

Ngày tải lên: 23/03/2014, 03:20

... Identification of a functional proximal promoter for X laevis Alignment of the 5¢-flanking region of exon IB from the XlMGP gene with the 5¢-flanking regions of ATGcontaining exons of mouse, rat ... the vertebrate homologue of the Drosophila protein zfh-1 [19], a factor containing both zinc finger and homeodomain motifs It is a 124 kDa DNA-binding protein which was initially characterized ... concentrations of available MGP protein Indeed, the presence of a maternally inherited MGP transcript (IB) in the first stages of Xenopus development may indicate that the MGP protein is required...

Ngày tải lên: 30/03/2014, 16:20

... mPER1 contains an additional NLS in its C-terminal domain (A) Mapping of NLS function in murine Per proteins Fragments corresponding to different portions of mPER1, mPER2 and mPER3 (as indicated) ... nuclear injection of mPER3, the protein is only slowly exported and there is no indication of protein degradation in the nucleus Thus, in summary, microinjection of individual isolated murine PER ... responsible for the masking of the NLS in mPER3 remains to be elucidated The NLS in mPER3 may be masked by intramolecular protein folding or by interaction with an unknown inhibitory factor With...

Ngày tải lên: 30/03/2014, 20:20

... microinjection 23! Culturing of Xenopus laevis embryos 23! Microinjection of synthetic mRNA into Xenopus laevis embryos 24! Harvesting RNA from Xenopus laevis embryos 27! ... certain types of analysis, such as promoter analysis, impossible in Xenopus laevis While the full genome sequence of Xenopus laevis is not available, the sequence of its close diploid cousin, Xenopus ... microinjection of synthetic mRNA Directly injecting mRNA into the developing embryo allows a researcher to perturb a developmental gene network by overexpressing a gene, introducing a dominant-negative,...

Ngày tải lên: 24/08/2014, 12:29

... subjects representing a community of Afghanistan that has experienced decades of conflicts with regard to a wide range of determinants of mental well-being The achieved response rate of 83.7% was ... sensitivity of women in Afghanistan who generally refrain from communicating with males who are not relatives, each study team consisted of one male and one female interviewer, both of whom were trained ... regarding means to restore the quality of life of people who were incapable of fleeing from long periods of conflict The objectives of this study were to examine the associations between exposure...

Ngày tải lên: 18/06/2014, 22:20

... wake at a.m., but nap for two hours or so in the early afternoon Thus the influence on one's sleep pattern is worthy of consideration when choosing an occupation ...

Ngày tải lên: 04/10/2012, 10:02

... expectation 1.What you think of the games your teachers used in your vocabulary lesson? a b c d Very interesting and useful Rather interesting and useful Interesting and useful Uninteresting and useless ... learners by bringing fun to classes Games are highly motivating because they are amusing and interesting (Ersoz, 2000)  By bringing fun to the language classrooms, games in vocabulary teaching will ... their meanings 1.1.4 Words use 1.1.5 Form of words 1.2 Vocabulary in language teaching and learning 1.2.1 A neglect aspect of foreign language methodology 1.2.2 Ineffective vocabulary learning 1.2.3...

Ngày tải lên: 29/01/2014, 14:36

... of occurrences in the test data for trigrams that appeared 10 times in training data Discounting by a single value is plausible in the case of similar train and test corpora, where the mean of ... this second point in constructing our language model, an advantage of our approach over subsampling is that we use our entire training corpus, and in so doing compromise between minimizing errors ... aggressive closing of the vocabulary Moore and Lewis (2010) achieve lower perplexities, but they use in- domain training data that we not include in our setting We briefly highlight some interesting features...

Ngày tải lên: 20/02/2014, 04:20

... the intervening dialogue renders the solution size determinate In out of the indeterminate antecedent AD+Os, our hypothesis is verified (see excerpt (1), where [35] is an AD+ with indeterminate ... exist to the set of constraint equations, each varl in the set of equations must have a solution For example, if instances of sofas are known for varsola, but every assignment of a value to varsoIa ... considers joint actions as decomposable into in- dependent Influence-on-Speaker / Hearer dimensions 4Space constraints prevent discussion of segments and it is 1) past; or 2) non past, and contains...

Ngày tải lên: 20/02/2014, 18:20

... S1) In addition, the ORF contains two arginine ⁄ glycine-rich (RGG) motifs that are characteristic of RNA-binding proteins, and an alanine-rich carboxy-terminal sequence that could be involved in ... that are important in recruiting XGld2 to CPE-containing mRNA, although other RNA-binding proteins may also be involved In vitro studies have shown that XGld2 is involved in cytoplasmic polyadenylation ... regulator of the transcriptional activity of the human oestrogen receptor [26] In this study, XRbm9 was identified as a new XGld2-interacting protein This RNA-binding protein is only detected in the...

Ngày tải lên: 07/03/2014, 05:20

... granules in skin melanophores resulting in darkening of the skin [23] POMC is the major cargo protein in this cell type and during adaptation to a black background the amount of POMC mRNA is induced ... are now in the position to obtain more understanding of the normal physiological role of PrPC, e.g by examining the effect of mutant PrPC proteins on melanotrope cell functioning, including copper ... (University of Leuven, Belgium [56]), and against the protein-folding chaperones calnexin and binding protein BiP by K Geering (University of Lausanne, Switzerland [57]) Monoclonal anti-tubulin IgG...

Ngày tải lên: 16/03/2014, 14:20

... exon encoding the KPI domain and the lack of a second heparin-binding domain [3,4,13,26] Comparative analysis of the Xenopus and mammalian APLP2 proteins Comparing the amino acid sequences of the ... (encoding a 12 amino acid domain involved in the attachment of a chondroitin sulfate glycosaminoglycan side chain) are alternatively spliced [29] While forms of APP mRNA lacking the KPI domain are ... residues in the aminoterminal part of APLP2 are present in X-APLP2 The zinc-binding domain consensus sequence (GxExVCCP [13]) in the N-terminal part of APLP2 is conserved between Xenopus and mammals...

Ngày tải lên: 16/03/2014, 16:20

... for inhibitor of apoptosis protein) domain is an evolutionarily conserved zinc-binding motif Many, but not all, proteins containing the BIR domain (BIR family proteins) play important roles in ... are distinct proteins rather than the nonallelic isoforms of a single protein in pseudotetraploid X laevis Another cDNA encodes a 401-residue protein containing two N-terminal BIR domains and ... for Xenopus survivin2, encodes a similar but distinct molecule of 157 aminoacid residues containing a single BIR domain As a very similar protein, named SIX, was recently identified as an RXRa-interacting...

Ngày tải lên: 30/03/2014, 16:20

... and Determination of the Insrinsic Lattice Constants of GaN Free from the strain Jpn J Appl Phys 1992, 31:L.1454 Tripathy S, Chua S, Chen P, Miao Z: Micro-Raman investigation of strain in GaN and ... existence of tensile stress and exceeding the critical thickness during the growth process We should explain the origin of the tensile stress Many authors observed intrinsic tensile stress using in ... in agreement with the variation of the linewidths The determined stress is the sum of the intrinsic and extrinsic at room temperature The stress is affected by lattice mismatch, coefficient of...

Ngày tải lên: 21/06/2014, 06:20

... to join in our investigation All of them possess a great deal of teaching experience, at least approximately five years of teaching Many of them have participated in workshops and training sessions ... benefits of having students work in pairs or groups? Shown in Table Q13 In your opinion, what are the obstacles in the implementation of pair work and group work in your speaking classes? Shown in ... this study is aimed at investigating the outcome of the implementation of pair work and group work in teaching speaking for the first year students of FFL- IUH and pointing out the merits and...

Ngày tải lên: 30/06/2014, 16:53

... stated, in his Marjolin-Prize winning contribution, that increased competition, ongoing consolidation, continuing pressure for the reduction of existing excess capacity and shrinking profitability in ... reducing, then eliminating inflation, – developing rules for non-monetary policies, then scope for coordinating them without undermining the rules, – developing a potential role in the international ... editing the final version and monitoring the printing process All the remaining errors are mine JPA An Overall Presentation 15 of the dominant themes and the title of a colloquium have been influenced...

Ngày tải lên: 23/07/2014, 09:52

... if there was evidence of excessive activity (including a premature eyeblink) during the baseline period They were also rejected if there was no evidence of an eyeblink having been evoked by the ... participated in the design of the study, helped to interpret statistical findings and to draft the manuscript All authors read and approved the final manuscript Page of Competing interests The ... modulation in a pictureviewing paradigm in control participants, but not in left or right temporal lobectomy patients Their data as well as ours indicate the involvement of both hemispheres in affective...

Ngày tải lên: 09/08/2014, 01:21