figure 6 5 group vi
Optimization of 293 HEK suspension cultures for adenovirus production
... Genes (Figure 6. 5, group II) 99 TCA Cycle and Electron Transport Chain Genes (Figure 6. 5, group III) 100 Glycolysis Genes (Figure 6. 5, group V) 102 Cell Cycle Genes (Figure ... 6. 2.3 6. 2.4 6. 2 .5 6. 2 .6 6.3 Ontological Distribution of Significantly Regulated Genes 86 Clustering of Significantly Regulated Genes 89 Amino Acid Metabolism Genes (Figure 6. 5, group ... PF-CDM in Shake Flask 75 5.4 Cell Growth and Metabolism in PF-CDM Batch and Fed-batch Cultures 75 iv 5. 5 5 .6 Summary of Cell Growth and Virus Productivity 81 5. 7 Virus Production in PF-CDM...
Ngày tải lên: 15/09/2015, 17:09
Báo cáo y học: "Relationship between Anti-CCP Antibodies and Oxidant and Anti-Oxidant Activity in Patients with Rheumatoid Arthrit"
... 24 25 24 25 24 25 24 25 24 25 24 25 24 25 24 25 Serum GSHpx (U/ml) Mean 0.47 0.48 14.10 15 .63 0.11 0.13 114.23 112.21 3.34 3 .52 50 .39 53 .63 0 .64 0 .62 67 .44 71.13 Std Deviation 0. 25 0.19 6. 67 4.39 ... Anti-CCP(+) (n= 25) (mean ±SD) 54 .4 ± 9 .6 18/7 24 .5 1.4 9 .6 ± 6. 3 34.7 ± 33 .6 5. 7 ± 5. 2 4.1± 1.8 37.9 ± 26. 8 34.3 ± 23.8 Anti-CCP(-) (n=24) (mean ±SD) 56 . 2 ± 11.2 19 /5 25. 3±1.9 8.1 ± 7 .5 17.8 ± 23 .5 2.8± ... Deviation p value 24 25 24 25 24 25 24 25 0.072 0.074 0.1 56 0.1 45 2. 852 4 .59 2 3.134 4.783 0.027 0.032 0.088 0.0 86 0 .50 9 0.780 2.1 25 2 . 65 4 0.432 0.324 0.021* 0.037* *p
Ngày tải lên: 25/10/2012, 11:15
Báo cáo y học: "Serum cholesterol concentration associated with aspirin esterase activity in older people: preliminary data"
... significant levels: 5. 7 ± 1.1 to 5. 8 ± 1.1 mmol/L in total cholesterol, 5 .6 ± 0.8 to 5. 5 ± 1.1 mmol/L in glucose, 41.4 [ 36. 0 -50 .0] to 39.8 [34 .6- 48.1] nmol/mL/min in aspirin esterase activity At baseline ... -0.2 76 (0. 268 ) Sex (men) 0. 352 (0. 152 ) Δ Body mass index (kg/m2) -0. 154 (0 .54 2) Δ Total cholesterol (mmol/L) 0 .54 2 (0.020)* Δ Glucose (mmol/L) 0.249 (0.320) β (P value) -0.389 (0.100) 0.3 06 (0.173) ... contained Tris-HCl (0 .6 mol/L) and CaCl2 (0.4 mol/L) in pH 7 .6- 7.7 Substrate was 55 5 mmol/L of acetylsalicylic acid (1g in 10 mL 100 % ethanol was used as the mother solution) Briefly, 15 µL of serum...
Ngày tải lên: 26/10/2012, 09:39
Báo cáo y học: "Matrix Metalloproteinase Activity in Pediatric Acute Lung Injur"
... non-viral ALI subjects (viral ALI MMP-8=49±22 ng/mg, non-viral ALI MMP-8=24 ±9 ng/mg; p≈ 0.3; viral ALI MMP-9 =58 2 ± 300 ng/mg, non-viral ALI MMP-9=402 57 ng/mg; p≈ 0.4) Figure 2: Expression of ... activities in virus-induced ALI compared with non-viral ALI We found insignificant elevations in total MMP-8 and activities in subjects with viral ALI (RSV=4, Parainfluenza=2) compared to non-viral ... Steroids Multi Organ Failure Death 25 17 159 ± 13 †Prolonged ALI 12 143 ± 17 24 ± 29 ± yr (1mo - 18yr) 15/ 10 4 .6 yr (1mo - 13yr) 7 /5 Pneumonia (8) Sepsis (6) Asthma (4) Bronchiolitis (2) Aspiration...
Ngày tải lên: 03/11/2012, 11:48
Tài liệu Sport and Physical Activity in Children with Congenital Heart Disease ppt
... findings) 1.3; 5. 1; 5. 2; 5. 3 No competitive sports D Severe (remaining) findings 1.4a; 1.4b; 3; 4.1; 5. 4; (6) Limited sports E Vitally threatening findings Dtsch Arztebl 2007; 104(9): A 56 3 –9 ⏐ www.aerzteblatt.de ... term/permanent threapy 5. 1 Anticoagulants 5. 3 Antiarrthythmics 5. 4 Group Pacemaker 5. 2 Anticongestives Patients after heart transplantation Dtsch Arztebl 2007; 104(9): A 56 3 –9 ⏐ www.aerzteblatt.de ... Coll Cardiol 19 85; 6: 11 86 232 Graham TP Jr, Driscoll DJ, Gersony WM, Newburger JW, Rocchini A, Towbin JA: Task Force 2: congenital heart disease J Am Coll Cardiol 20 05; 45: 13 26 33 10 Reybrouck...
Ngày tải lên: 12/02/2014, 19:20
Tài liệu Báo cáo khoa học: Exposure of IgG to an acidic environment results in molecular modifications and in enhanced protective activity in sepsis doc
... T (20 05) The autoreactivity of therapeutic intravenous immunoglobulin (IVIG) preparations depends on the fractionation methods used Scand J Immunol 61 , 357 – 363 Notkins A (2004) Polyreactivity ... Science 2 86, 21 56 2 159 11 Zhou Z, Zhang Y, Hu Y, Wahl L, Cisar J & Notkins A (2007) The broad antibacterial activity of the natural Molecular modifications in low pH-exposed IgG 12 13 14 15 16 17 18 ... later, groups of mice ( 15 per group) were injected intravenously with increasing doses of the native IVIg or of the low-pH buffer-exposed IVIg preparation, or with NaCl ⁄ Pi alone Survival was...
Ngày tải lên: 16/02/2014, 15:20
Tài liệu Báo cáo khoa học: Mouse recombinant protein C variants with enhanced membrane affinity and hyper-anticoagulant activity in mouse plasma pptx
... V Mutant VI Mutant VII 0-10-90 20-10-70 nM g – 7. 26 5. 55 0 .66 1.32 7.10 2. 05 7.47 2 .55 – NAd NA 2. 45 8.70 NA 8.40 NA 8.77 – 8.77 4. 96 0 .67 1.44 6. 79 2.28 5. 99 2 .67 2 76 241 1 05 1 65 15 247 24 ± ... ± 0 .50 c 0. 26 0.04 0. 16 0.71 0.20 0. 46 0. 25 ± 0.13 ± 0.98 ± 1. 15 ± 0. 75 ± ± ± ± ± ± ± ± 0. 75 0. 16 0. 05 0.18 0. 45 0.17 0. 45 0.23 ± ± ± ± ± ± ± ± ± 32 20 28 25 18 18 26 56 7 7 53 80 2813 737 65 8 4094 ... protein C for mouse (NP_032 960 .2), human (NP_000303.1), rat (NP_0 369 35. 1), bovine (XP _58 5990.3) and human prothrombin (NP_000497.1) 65 88 FEBS Journal 2 76 (2009) 65 86 66 02 ª 2009 The Authors Journal...
Ngày tải lên: 18/02/2014, 06:20
Tài liệu An Empirical Analysis of Political Activity in Hollywood pptx
... Kevin Bacon Total Contributions 3 96, 000 76, 450 73,000 64 ,50 0 61 ,000 60 ,000 57 ,50 0 57 ,50 0 53 ,000 35, 000 34 ,50 0 30 ,50 0 30,000 29,000 27,000 25, 400 25, 000 24,000 19 ,50 0 18 ,50 0 18,000 16, 50 0 15, 500 ... of box office returns, starring roles (mil $) 36. 85 22. 15 48. 56 35. 32 37 .52 24.83 168 .09 1 06. 27 166 .99 1 26. 80 1 36. 01 97. 45 49.49 28 .63 52 .01 36. 42 45. 24 29.87 23 Table 2: Largest Contributors in ... 0. 15 0.14 Age at first film 22.97 6. 12 Age at first starring role 25. 41 6. 73 Total # films 24.30 Top 75 box office films 12. 45 46. 95 11.18 27. 16 5. 92 15. 39 15. 46 10.87 7.19 5. 77 6. 31 5. 92 Top 25...
Ngày tải lên: 19/02/2014, 10:20
Tài liệu Visual Activity in Hollywood Film: 1935 to 2005 and Beyond docx
... escape 24.4 0 .6 10 .5 66 .7 90.8 57 .7 83.9 58 .2 75. 7 104.1 69 .6 101.7 23.3 91.9 70.9 100.7 42.7 94.2 97 .6 85. 9 111.9 74.0 4.3 2.9 4.4 4.3 4.0 6. 3 7.0 3.8 3.4 4 .6 32 .6 7.8 5. 9 3.0 45. 0 13.9 7 .6 4.8 11.8 ... 0 .5 76 0.290 0.3 56 0. 354 0.219 0.384 0.377 0.1 96 0.4 35 0.390 0.240 0. 257 0.249 0.297 0.199 0.173 0. 160 0.199 0.190 0.307 0. 150 0. 169 19.7 0.8 0.8 1.1 1.0 1 .6 1.3 2.1 3.0 1.9 2.7 1.4 4.1 3.3 3 .6 ... plot of whole-film visual activity indices (VAIs) by year for 1 45 films from 19 35 to 20 05 VISUAL ACTIVITY IN HOLLYWOOD FILM Figure Scatter plots of whole-film visual activity indices (VAIs)...
Ngày tải lên: 19/02/2014, 14:20
Tài liệu Báo cáo khoa học: Polarized distribution of inducible nitric oxide synthase regulates activity in intestinal epithelial cells pdf
... Immunol 172, 3 051 –3 058 36 Kuo PC & Abe KY (19 95) Cytokine-mediated production of nitric oxide in isolated rat hepatocytes is dependent on cytochrome P- 450 III activity FEBS Lett 360 , 10–14 37 Courjault-Gautier ... subcellular location of nitrogen oxide synthases in RAW 264 .7 macrophages Mol Pharmacol 41, 61 5 62 4 10 Vodovotz Y, Russell D, Xie Q, Bogdan C & Nathan C (19 95) Vesicle membrane association of nitric oxide ... Immunol 154 , 2914–29 25 11 Webb JL, Harvey MW, Holden DW & Evans TJ (2001) Macrophage nitric oxide synthase associates with cortical actin but is not recruited to phagosomes Infect Immun 69 , 63 91 64 00...
Ngày tải lên: 19/02/2014, 16:20
Tài liệu Báo cáo khoa học: Experimental proof for a signal peptidase I like activity in Mycoplasma pneumoniae, but absence of a gene encoding a conserved bacterial type I SPase pdf
... Remarks 850 .2 855 .7 859 .7 4 36. 3 65 3.9 443 .6 448.9 444.2 66 3.8 454 .7 462 .7 470.7 799 .5 831.1 62 3.3 33 96. 6 3418 .6 3434 .6 13 05. 9 13 05. 7 1327.7 1343 .6 1329.7 13 25 .6 907.4 923.4 939.4 23 95. 5 2490.2 ... N(+1 36) TYLLQDHNTLTPYTPFTTPXDGGXDVVR ( 26 54 ) N(+1 36) TYLLQDHNTLTPYTPFTTPXDGGXDVVR ( 26 54 ) N(+1 36) TYLLQDHNTLTPYTPFTTPXDGGXDVVR ( 26 54 ) VGDTK(+1 36) XVAXVR (70–80) VGDTK(+1 36) XVAXVR (70–80) VGDTK(+1 36) XVAXVR ... VGDTK(+1 36) XVAXVR (70–80) VGDTKXVAXVR (70–80) RVGDTKXVAXVR (69 –80) ATWVFER (1 46 152 ) ATW(ox)VFER (1 46 152 ) ATW(2 · ox)VFER (1 46 152 ) TLQDLXVEQPVTPYTPNAGLAR (182–203) VDGVAQDTVHFGSGQESSWNSQR (204–2 26) ...
Ngày tải lên: 19/02/2014, 18:20
Tài liệu Báo cáo khoa học: "Mining metalinguistic activity in corpora to create lexical resources using Information Extraction techniques: the MOP system" doc
... 0. 85 0.98 IISMax W 31 86 0. 86 0.87 0. 95 GISMax W 3188 0. 86 0.87 0. 95 NB T 1 36 0.88 0.97 0.84 NB T 794 0.87 0. 96 0.84 NB W 4290 0.73 0. 86 0.77 F NB (3/W) IIS (3/W) R GIS (1/W) P 0 .6 0 . 65 0.7 0. 75 ... Precision W 1 254 0.97 0. 96 0.98 IISMax T 1 36 0. 95 0. 96 0.94 IISMax W 1 252 0.92 0.97 0.9 GISMax T 138 0.91 0.9 0. 96 GISMax T 7 96 0.88 0.93 0.92 IISMax T 794 0. 86 0. 95 0.89 IISMax W 4290 0.87 0. 85 0.98 ... an overall F-measure of 0.97 .6 Of 55 81 sentences ( 96 of which were metalinguistic sentences signaled by our cluster of verbs), 83 were extracted, with 13 (or 15 .6% of candidates) filtered-out...
Ngày tải lên: 20/02/2014, 15:20
Tài liệu Báo cáo khoa học: The molecular surface of proteolytic enzymes has an important role in stability of the enzymatic activity in extraordinary environments pptx
... D137N A 150 T S170N V177T E192G M196V Y 259 Q D 266 N Y 269 A Q272R D 266 N/Y 269 A A271T/Q272R Y 269 A/A271T/ Q272R D 266 N/Y 269 A/ A271T/Q272R s-N 263 D s-N 263 D/A 266 Y 5 -TTAACCTTCAtatgTGAGGGTATTTTTTG-3¢ 5 -TTTGCTTCTCAtatgTTACCCTCTCC -6 ... Y 269 A/A271T/Q272R D 266 N/Y 269 A/A271T/Q272R Wild-type Sendai N 263 D N 263 D/A 266 Y 0.290 0.237 0.282 0.310 0. 262 0.277 0.300 0. 2 65 0. 2 65 0. 360 0. 2 65 0.300 0.270 0.330 0.327 0.319 26. 2 21.2 21.1 25. 0 19.9 23.3 ... N 266 D- and N 266 D/Y 269 A-Sendai were constructed by using primers s-N 263 D and s-N 263 D/A 266 Y (Table 1) Compared with the stability of wild-type Sendai, both mutant enzymes, N 266 D- and N 266 D/Y 269 A-Sendai...
Ngày tải lên: 21/02/2014, 03:20
Triennial Central Bank Survey: Foreign exchange and derivatives market activity in April 2010 pptx
... institutions with non-financial customers 155 87 56 11 58 56 36 331 219 98 14 68 66 30 62 1 3 25 241 55 61 52 39 1,210 55 2 57 4 85 72 46 47 1,2 75 537 58 5 154 61 42 46 12 Options and other products² with ... customers 2 65 150 89 27 100 56 34 10 Local 133 50 207 42 414 40 56 4 33 762 37 Cross-border 132 50 282 58 60 9 59 1,120 66 1,321 63 489 100 323 66 142 29 25 1,0 25 100 494 48 450 44 79 1 ,68 6 100 800 ... 13 743 1,1 85 1,274 2, 051 1,3 95 2 ,5 86 Local Cross-border 69 8 828 38 61 38 62 35 65 Outright forwards Up to days Over days and up to year Over year 128 100 65 51 57 45 130 100 51 39 76 58 209 100...
Ngày tải lên: 06/03/2014, 02:21
Báo cáo khoa học: Interference with the citrulline-based nitric oxide synthase assay by argininosuccinate lyase activity in Arabidopsis extracts docx
... Desalted extract Desalted extract + ADF Desalted extract + treated ADF Activity 16 354 ± 1 267 1 762 ± 119 16 0 85 ± 1440 258 3 ± 183 Fig Fumarate can replace ADF as a cosubstrate for the reaction ... Biochem J 357 , 59 3 61 5 Stuehr DJ, Santolini J, Wang ZQ, Wei CC & Adak S (2004) Update on mechanism and catalytic regulation in the NO synthases J Biol Chem 279, 361 67– 361 70 Li H & Poulos TL (20 05) Structure–function ... Trends Plant Sci 11, 52 4– 52 5 51 Kim SC & Raushel FM (19 86) Isotopic probes of the argininosuccinate lyase reaction Biochemistry 25, 4744– 4749 52 Rosenthal GA & Naylor AW (1 969 ) Purification and...
Ngày tải lên: 07/03/2014, 05:20
Báo cáo khoa học: A new approach for distinguishing cathepsin E and D activity in antigen-processing organelles pdf
... Substrate + EF + PepA 5 10 10 10 15 15 15 20 20 20 25 25 25 30 30 30 35 35 35 J Substrate + CF (IP) K Substrate + LF (IP) L Substrate + EF (IP) 5 10 10 10 15 15 15 20 20 25 25 30 30 35 35 Relative Fluorescence ... Substrate + CF 20 25. 54 E Substrate + LF 25 F Substrate + EF 5 10 Elution time (min) 25. 19 10 10 15 15 15 20 20 25 25 30 30 35 35 G Substrate + CF + PepA 20 22.87 25. 90 22.38 25. 44 25 30 35 H Substrate ... 0. 76 0. 76 ND 1 06. 3 25. 3 80.9 20.9 9 .5 11.3 210.7 23.2 187 .5 82.0 65 .4 16. 6 13.7 11.8 1.9 232.8 2 25. 7 7.1 WT100 DCs ± 2. 15 ± 2. 15 ± 0.19 ± 0.19 ± 0.24 ± 0.24 and the quencher group Figure 6B shows...
Ngày tải lên: 07/03/2014, 09:20
Can’t Shake that Feeling: Event-Related fMRI Assessment of Sustained Amygdala Activity in Response to Emotional Information in Depressed Individuals pptx
... Empirical Left 3 56 637b 421 58 1a 51 1a 62 4a 292 373 1 76 303 Ϫ.048 Ϫ .52 1a 52 0a 461 334 491 63 8b 60 2a 484 323 214 1 96 Ϫ.1 35 Ϫ .51 7a 53 5a 58 8a 57 2a 731b 67 8b 68 2b 742b 359 350 2 05 088 Ϫ. 469 fMRI, functional ... 8, 61 19, 5, Ϫ12 Ϫ 15, Ϫ4, 6 Ϫ21, Ϫ10, Ϫ8 54 , Ϫ23, 32 4, Ϫ31, 18 p1 p p p p p p p Ͻ Ͻ Ͻ Ͻ Ͻ Ͻ Ͻ 01 05 05 1 p2 p p p p p p p Ͻ Ͻ Ͻ Ͻ Ͻ Ͻ Ͻ 05 05 05 01 05 05 05 Location Middle frontal gyrus BA 46 ... neutral words (M(SD) ϭ 1312 (60 4) ms) than to positive words (M(SD) ϭ 1 061 [ 463 ] ms, F(1, 16) ϭ 17.3, p ϭ 001) or negative words (M(SD) ϭ 1 163 (50 4) ms, F(1, 16) ϭ 6. 09, p ϭ 0 25) With the possible exception...
Ngày tải lên: 07/03/2014, 17:20
Báo cáo khoa học: Critical role of the plasma membrane for expression of mammalian mitochondrial side chain cleavage activity in yeast pptx
... strains (CA10/pCD63 + pCD69) (Derg5, expressing CYP11A1 and Tgl1p) and (CA10/pCD63 + pYeDP60) (Derg5, expressing CYP11A1) and in Dare Dare strains (CA23/pCD63 + pCD69) (Derg5, expressing CYP11A1 ... Biophys Res Commun 2 76, 210–2 15 61 Wu, D & Cederbaum, A.I (1992) Presence of functionally active cytochrome P- 450 IIE1 in the plasma membrane of rat hepatocytes Hepatology 15, 51 5 52 4 62 Sakaki, T., ... 16, 1 86 189 Yang, H., Bard, M., Bruner, D.A., Gleeson, A., Deckelbaum, R.J., Aljinovic, G., Pohl, T.M., Rothstein, R & Sturley, S.L 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 (19 96) ...
Ngày tải lên: 08/03/2014, 08:20
Báo cáo Y học: Restoring enzyme activity in nonfunctional low erucic acid Brassica napus fatty acid elongase 1 by a single amino acid substitution pdf
... 40 .64 40 .67 3.97 3 .62 3. 96 4. 75 4 .69 5. 88 15. 15 13.90 14.97 29.03 18 .63 24 .69 1 .52 1. 56 1. 46 1.02 1 .68 1.01 0. 45 0.49 0. 45 0.07 1 .67 0.00 0.99 1.10 0.94 0.00 3.17 0.00 2.07 2.19 1.94 0.00 0. 85 ... 16: 0 16: 1 18:0 18:1 D9 18:1 D11 20:1 D11 20:1 D13 22:1 D13 22:1 D 15 24:1 VLCMFA B n Hero B o 103 B r R500 B n Westar A t Col pYES2.1 16. 34 15. 77 15. 22 14.91 16. 12 19.22 43.27 44.84 43. 76 45. 50 ... primers PCR incubations were run 30 sec at 95 °C (denaturation) followed by 16 cycles of 30 sec at 95 °C, at 55 °C, 15 at 68 °C and terminated by 15 at 68 °C Following temperature cycling, the product...
Ngày tải lên: 08/03/2014, 09:20
Báo cáo khoa học: Catalytic mechanism of the primary human prostaglandin F2asynthase, aldo-keto reductase 1B1 – prostaglandin D2 synthase activity in the absence of NADP(H) pptx
... be pH 5. 0 for PGFS activity and pH 8 .5 for PGDS activity of AKR1B3 The pKb value of PGFS activity and the pKa value of PGDS activity were calculated by Eqn (1) to be 5. 39 ± 0.09 and 7 .57 ± 0.04, ... Prostaglandin D2 synthase activity of AKR1B1 27 28 29 30 31 32 33 34 35 36 37 aldose reductase Site-directed mutagenesis of Asp-43, Tyr-48, Lys-77, and His-110 J Biol Chem 268 , 2 56 8 7– 2 56 9 3 Fukunishi Y, Mikami ... 0.31 88 110 22 0 .69 45 27 5. 2 ± ± ± ± ± ± ± ± 0. 06 39 49 0. 16 9 1.0 catalyzed by the triad composed of Tyr48, Lys77 and His110 and assisted by ionic interaction with Asp43 [ 25, 26] , and suggest...
Ngày tải lên: 14/03/2014, 23:20