... experimental groups Septic shock was induced in 16 18week-old animals by the intraperitoneal administration of 400 lg of bacterial LPS (from E coli B 055 :B5, SigmaAldrich, #L2880) Minutes later, groups ... Science 2 86, 21 56 2 159 11 Zhou Z, Zhang Y, Hu Y, Wahl L, Cisar J & Notkins A (2007) The broad antibacterial activity of the natural Molecular modifications in low pH-exposed IgG 12 13 14 15 16 17 18 ... 121, 253 –2 56 Matulis D, Baumann C, Bloomfield V & Lovrien R (1999) 1-Anilino-8-naphthalene sulfonate as a protein conformational tightening agent Biopolymers 49, 451 – 458 Sudhakar K & Fay P (19 96) ...
... Immunol 172, 3 051 –3 058 36 Kuo PC & Abe KY (19 95) Cytokine-mediated production of nitric oxide in isolated rat hepatocytes is dependent on cytochrome P- 450 III activity FEBS Lett 360 , 10–14 37 Courjault-Gautier ... 272 (20 05) 444– 453 ª 2004 FEBS Apical iNOS dimer in epithelial cells Miller MJS & Sandoval M (1999) Nitric Oxide III A molecular prelude to intestinal inflammation Am J Physiol 2 76, G7 95 G799 Andre ... Immunol 154 , 2914–29 25 11 Webb JL, Harvey MW, Holden DW & Evans TJ (2001) Macrophage nitric oxide synthase associates with cortical actin but is not recruited to phagosomes Infect Immun 69 , 63 91 64 00...
... M196V Y 259 Q D 266 N Y 269 A Q272R D 266 N/Y 269 A A271T/Q272R Y 269 A/A271T/Q272R D 266 N/Y 269 A/A271T/Q272R Wild-type Sendai N 263 D N 263 D/A 266 Y 0.290 0.237 0.282 0.310 0. 262 0.277 0.300 0. 2 65 0. 2 65 0. 360 ... D137N A 150 T S170N V177T E192G M196V Y 259 Q D 266 N Y 269 A Q272R D 266 N/Y 269 A A271T/Q272R Y 269 A/A271T/ Q272R D 266 N/Y 269 A/ A271T/Q272R s-N 263 D s-N 263 D/A 266 Y 5 -TTAACCTTCAtatgTGAGGGTATTTTTTG-3¢ 5 -TTTGCTTCTCAtatgTTACCCTCTCC -6 ... N 266 D- and N 266 D/Y 269 A-Sendai were constructed by using primers s-N 263 D and s-N 263 D/A 266 Y (Table 1) Compared with the stability of wild-type Sendai, both mutant enzymes, N 266 D- and N 266 D/Y 269 A-Sendai...
... 40 .64 40 .67 3.97 3 .62 3. 96 4. 75 4 .69 5. 88 15. 15 13.90 14.97 29.03 18 .63 24 .69 1 .52 1. 56 1. 46 1.02 1 .68 1.01 0. 45 0.49 0. 45 0.07 1 .67 0.00 0.99 1.10 0.94 0.00 3.17 0.00 2.07 2.19 1.94 0.00 0. 85 ... 16: 0 16: 1 18:0 18:1 D9 18:1 D11 20:1 D11 20:1 D13 22:1 D13 22:1 D 15 24:1 VLCMFA B n Hero B o 103 B r R500 B n Westar A t Col pYES2.1 16. 34 15. 77 15. 22 14.91 16. 12 19.22 43.27 44.84 43. 76 45. 50 ... primers PCR incubations were run 30 sec at 95 °C (denaturation) followed by 16 cycles of 30 sec at 95 °C, at 55 °C, 15 at 68 °C and terminated by 15 at 68 °C Following temperature cycling, the product...