fibroblast growth factor and other factors

... [18] and low oxygen tension (mild hypoxia) [19-21] Figure In the present study we have investigated the presence of chondrogenic growth factors and hypoxia with human meniscus cells expanded ... from fibroblast growth factor (FGF2)-expanded versus non-FGF2-expanded cells P2, passage 2; P3, passage AGCTTCTGTGGAACCATGGAA-3' (reverse); COL2A1, 5'CTGCAAAATAAAATCTCGGTGTTCT-3' (forward) and ... from fibroblast growth factor (FGF2)expanded versus non-FGF2-expanded cells Page of (page number not for citation purposes) Arthritis Research & Therapy Vol No Adesida et al Figure Weights and...

Ngày tải lên: 09/08/2014, 07:20

... collagen type I and II, aggrecan, and control IgG in cell aggregates following chondrogenic differentiation for 14 days in IPFP cells expanded with and without fibroblast growth factor- 2 (FGF-2)-supplemented ... present in the IPFP tissue of fibroblast growth factor- 2 (FGF-2) expansion Chondrogenic cultures of infrapatellar fat pad (IPFP) cells and effects of fibroblast growth factor- 2 (FGF-2) expansion ... and the IgG control The FGF-2-expanded cells are morphologically different from cells expanded in the absence of FGF-2 but show a similar cell surface expression Effect of fibroblast growth factor- 2...

Ngày tải lên: 09/08/2014, 10:23

... of growth factors (for example Angiopoietin-1, Platelet derived growth factor, Hypoxia inducing factor- 1a, EphrinB2, Nitric Oxygen Synthase) have been found to play significant roles; VEGF and ... of the growth factors bFGF and VEGF and, hence, to explain the mechanism of its therapeutic use Methods Between May and October 1999, specimens were obtained from 86 persons: 43 patients and 43 ... [Predictive value of serum levels of basic fibroblast growth factor, vascular endothelial growth factor and matrix metalloproteinase-2 in advanced carcinomas of the head and neck] HNO 1999, 47:695-701 21...

Ngày tải lên: 11/08/2014, 20:20

... and/ or growth factors (10 ng/ml), and supernatants analyzed by ELISA Mean & SEM, n = RA FLS lines Asterisk indicates P < 0.05 between the combination and the added values for TNF alone and growth ... Since the mRNA and protein results essentially mirrored each other (compare for example Figures and 4, and Figures 5a and 5b), the underlying reason for the synergy of the two growth factors along ... SN: Identification of the major fibroblast growth factors released spontaneously in inflammatory arthritis as platelet derived growth factor and tumour necrosis factor- alpha Clin Exp Immunol 1991,...

Ngày tải lên: 12/08/2014, 12:20

... I, Yagel S: Vascular endothelial growth factor, epidermal growth factor and fibroblast growth factor- 4 and -10 stimulate trophoblast plasminogen activator system and metalloproteinase-9 Mol Hum ... Medium (DMEM) and foetal bovine serum (FBS) were from PAA (Lodz, Poland) Recombinant human vascular endothelial growth factor (rhVEGF-A) and recombinant human fibroblast growth factor (rhFGF4) ... Fibroblast growth factors and their receptors Biochem Cell Biol 1997, 75(6):669-685 Zhang X, Ibrahimi OA, Olsen SK, Umemori H, Mohammadi M, Ornitz DM: Receptor specificity of the fibroblast growth...

Ngày tải lên: 14/08/2014, 19:22

... concentration after stimulation with EGF and PMA together on the one hand, and the sum of the ERK-PP concentrations after stimulation with EGF and PMA separately on the other hand, was not constant, but increased ... independent experiments, and as we wanted to compare them to each other and calculate the standard errors for each time point, the individual time curves had to be scaled to each other Scaling the curves ... phosphorylates MAPK/ERK kinase (MEK) and 2, which in turn phosphorylate ERK1 and ERK2 Phosphorylated ERK (ERK-PP) has several different cytoplasmic and nuclear targets Transcription factors activated by ERK-PP...

Ngày tải lên: 19/02/2014, 16:20

... hepatocyte growth factor by the plasminogen activators uPA and tPA Am J Pathol 143, 949958 Thewke, D.P & Seeds, N.W (1996) Expression of hepatocyte growth factor/ scatter factor, its receptor, c-met, and ... plasminogen-like growth factors HGF and MSP are very similar to Plg in their domain structure and each of them contains four kringle domains Prothrombin has two kringle domains and the structures ... to Plg, tPA, and HGF, whereas uPA, prothrombin, and MSP showed no binding properties towards TN Distinct bands of Plg and HGF were visible after a short time of exposure The tPA band was somewhat...

Ngày tải lên: 17/03/2014, 03:20

... different specificities for the ligands, EGF and NRG, respectively, but are very similar in their other properties Actually, both ErbB-1 and ErbB-4 have a ligand-promoted tyrosine kinase activity, ... even in the bivalent manner of EGF binding On the other hand, chimeras 4111-1 and 4411-1, generated by the replacement of domain I and of domains I and II, respectively, of ErbB-1 by the corresponding ... Driscoll, P.C & Campbell, I.D (1992) Human epidermal growth factor High resolution solution structure and comparison with human transforming growth factor a J Mol Biol 227, 271–282 Kohda, D & Inagaki,...

Ngày tải lên: 24/03/2014, 03:21

... Abbreviations: FGF, fibroblast growth factor; FGFR, fibroblast growth factor receptor; NR, not reached; PDGF, platelet-derived growth factor; VEGFR, vascular endothelial growth factor receptor; ... VEGFR-3 Abbreviations: FGF, fibroblast growth factor; FGFR, fibroblast growth factor receptor; PDGF, platelet-derived growth factor; VEGFR, vascular endothelial growth factor receptor (B) FGF2 uPa ... extracellular matrix; FGF, fibroblast growth factor; FGFR, fibroblast growth factor receptor; PDGF, platelet-derived growth factor; PDGFR, platelet-derived growth factor receptor; MMP, matrixmetallo...

Ngày tải lên: 18/06/2014, 19:20

... Abbreviations: FGF, fibroblast growth factor; FGFR, fibroblast growth factor receptor; NR, not reached; PDGF, platelet-derived growth factor; VEGFR, vascular endothelial growth factor receptor; ... VEGFR-3 Abbreviations: FGF, fibroblast growth factor; FGFR, fibroblast growth factor receptor; PDGF, platelet-derived growth factor; VEGFR, vascular endothelial growth factor receptor (B) FGF2 uPa ... extracellular matrix; FGF, fibroblast growth factor; FGFR, fibroblast growth factor receptor; PDGF, platelet-derived growth factor; PDGFR, platelet-derived growth factor receptor; MMP, matrixmetallo...

Ngày tải lên: 20/06/2014, 04:20

... regeneration and wound healing by releasing the growth factors in a controlled and temporal manner Base a longer release period, around weeks [13] However, it was difficult to control the release rate and ... nanoparticles offer prolonged releasing kinetics Other possible factors related to the releasing rate include the pore size and the properties of payload, and so on The result as shown in Fig indicates ... the living cell and dead cells were measured at an excitation of 400 nm and an emission of 505 nm, then at an excitation of 485 nm and an emission of 520 nm, respectively Results and Discussion...

Ngày tải lên: 22/06/2014, 00:20

... tumor necrosis factor- α, vascular endothelial growth factor1 65 and SU1498 on ADAM15 expression (a) Effect of tumor necrosis factor expression (TNF)-α and vascular endothelial growth factor (VEGF)165 ... major immunoreactive bands of 100, 76, 66, 47, 41, 38, 34 and 29 kDa were observed (Fig 6, lane 2) Because the molecular weight of the 100-kDa band Effect of cytokines and growth factors on ADAM15 ... VEGFR-2 and neuropilin-1 expression by cytokines and growth factors in RA SFs and HUVECs As previously reported [15,29], HUVECs expressed the three major VEGF receptors (VEGFR-1, VEGFR-2 and neuropilin1),...

Ngày tải lên: 09/08/2014, 07:20

... include proinflammatory cytokines and growth factors such as IL-1 [5,11,12] – and potentially fibroblast growth factor (FGF2) (otherwise known as basic fibroblast growth factor) [13] – both of which ... EO: Growth factor expression in degenerated intervertebral disc tissue An immunohistochemical analysis of transforming growth factor beta, fibroblast growth factor and platelet-derived growth factor ... regulators of IVD homeostasis include polypeptide growth factors, such as insulin-like growth factor 1, transforming growth factor beta (TGFβ) and the bone morphogenetic proteins (BMPs) [7] In...

Ngày tải lên: 09/08/2014, 10:23

... synoviocytes, fibroblasts, or other cells FGF8 can induce cartilage and bone degradation to cause the arthritis-like syndromes and possibly aggravate the pathology of OA Other factors such as ... signaling is transduced through the formation of a complex of a growth factor, a proteoglycan, and a high-affinity fibroblast growth factor receptor (FGFR), which is a transmembrane tyrosine kinase ... mesodermal and ectodermal origin [7,8] FGF8 was originally isolated from the conditioned medium of an androgen-dependent mouse mammary tumor cell line (SC3) as an androgen-induced growth factor, and...

Ngày tải lên: 09/08/2014, 10:23

... (nerve growth factor (NGF), brain-derived growth factor (BDNF), and neurotrophin (NT)-3, NT-4) and their corresponding receptors (high-affinity receptors tyrosine kinase (trk)A, trkB, trkC and the ... expression levels of the four neurotrophic ligof the neurotrophic ligands ands (a) nerve growth factor (NGF) (b) Brain-derived growth factor (BDNF) (c) Neurotrophin (NT)-3 (d) NT-4) Bold horizontal ... D'Elios MM, De Carli M, Aloe L: Human CD4+ T cell clones produce and release nerve growth factor and express high-affinity nerve growth factor receptors J Allergy Clin Immunol 1997, 100:408-414 Bonini...

Ngày tải lên: 09/08/2014, 14:21

... ECM rich in proteoglycans and collagens This matrix is continuously remodelled in a process controlled by the NP cells and closely regulated by anabolic growth factors and catabolic cytokines In ... expression and correlation of nerve growth factor and substance P in human nucleus pulposus NGF and substance P demonstrated similar levels of expression between PM normal and PM degenerate samples but ... degenerate and surgical degenerate NP samples Scatterplots were initially drawn to assess correlations between expression of MMP-10 and IL-1, TNF-α or NGF expression and between NGF and substance P, and...

Ngày tải lên: 09/08/2014, 14:22

... ERK and JNK-p38 MAP kinases on apoptosis Science 1995, 270:1326-1331 Wert MM, Palfrey HC: Divergence in the anti-apoptotic signalling pathways used by nerve growth factor and basic fibroblast growth ... PC12 cells Comparison of the effects of nerve growth factor and epidermal growth factor Biochem J 1992, 288 ( Pt 2):351-355 Publish with Bio Med Central and every scientist can read your work free ... was performed Our results on growth factor stimulated PC12 cells show that both the anti-apoptotic effect and the activation of ERK1/2 and Akt were transient and minor when compared to NGF It remains...

Ngày tải lên: 11/08/2014, 08:20

... Real-Time PCR probe and primers name, symbol and ID Gene Symbol Assay ID Vascular endothelial growth factor A Vascular endothelial growth factor B Vegfa Vegfb Placenta growth factor Pgf Rn00585926_m1 ... hypoxia-mediated growth factors in a novel bladder cancer animal model Anticancer Res 2007, 27(6B):4225-31 52 Itakura E, et al: Detection and characterization of vascular endothelial growth factors and their ... Purification and characterization of a naturally occurring vascular endothelial growth factor. placenta growth factor heterodimer J Biol Chem 1995, 270(13):7717-23 Eriksson A, et al: Placenta growth factor- 1...

Ngày tải lên: 12/08/2014, 13:22

... inflammatory and proliferative cascades of PsA and RA Abbreviations ELISA, enzyme-linked immunosorbent assay; FLS, fibroblast- like synoviocyte; NGF, nerve growth factor; NGF-R, nerve growth factor ... Nerve growth factor and receptor expression in rheumatoid arthritis and spondyloarthritis Arthritis Res Ther 2009, 11:R82 Raychaudhuri SP, Raychaudhuri SK: The regulatory role of nerve growth factor ... expressed as mean ± standard deviation Nerve growth factor (NGF), TrkA, and p75 data of unstimulated FLSs were compared with NGF, TrkA, and p75 data of FLSs of other groups mentioned in the table a/bSignificantly...

Ngày tải lên: 12/08/2014, 14:21

... Fibroblast growth factor receptor is principally responsible for fibroblast growth factor 2-induced catabolic activities in human articular ... multifactorial heterogeneous disease that is influenced by both genetic and environmental factors [1] A wide array of enzymes, such as matrix metalloproteinases (MMPs) and a disintegrin and metalloproteinase ... 44:585-594 Loeser RF, Chubinskaya S, Pacione C, Im HJ: Basic fibroblast growth factor inhibits the anabolic activity of insulin-like growth factor and osteogenic protein in adult human articular chondrocytes...

Ngày tải lên: 12/08/2014, 17:22