fibrobasts epithelial cells and endothelial cells

Báo cáo y học: " Influenza H5N1 virus infection of polarized human alveolar epithelial cells and lung microvascular endothelial cells" pdf

Ngày tải lên : 12/08/2014, 14:20

... two principal cell types: flattened type I alveolar epithelial cells and cubodial type II alveolar epithelial cells Type I alveolar epithelial cells cover over 80% of the alveolar surface in which ... alveolar epithelial cells and HLMVEs Lectin binding assay to determine the Sias distribution on polarized type I-like alveolar epithelial cells and HLMVEs (A, C, E) SNA binds to Siaa2-6Gal and (B, ... virus infection and replication in alveolar epithelial cells Both influenza A viruses, A/HK/54/98 (H1N1) and A/HK/ 483/97 (H5N1) were able to infect the type I-like alveolar epithelial cells from...

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Tài liệu Báo cáo khoa học: Role for nectin-1 in herpes simplex virus 1 entry and spread in human retinal pigment epithelial cells docx

Ngày tải lên : 18/02/2014, 14:20

... representing entry into RPE cells were very similar to those seen with HeLa and Vero cells Both HeLa and Vero cell are naturally susceptible and frequently used for examining entry and virus propagation ... experiments HVEM-expressing CHO-K1 cells (control) and RPE cells were positive for HVEM expression (Fig 4B) Similarly, nectin-1-expressing CHO-K1 cells and also RPE cells were positive for nectin-1 ... h window of infection We infected RPE cells with K26GFP [27], and stained cells for F-actin, using phalloidin at 30 and h postinfection, and examined the cells under a high-resolution confocal...

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Tài liệu Báo cáo khoa học: Caveolin-1 inﬂuences P2X7 receptor expression and localization in mouse lung alveolar epithelial cells docx

Ngày tải lên : 19/02/2014, 00:20

... Caveolin-1 and ﬂotillin-1 (Flo-1), and the nonraft marker T1a (an integral membrane protein expressed strongly within the apical plasma membrane of both type I alveolar epithelial cells and lymphatic endothelial ... immunoreactivity in both type I cells and pulmonary endothelial cells (Fig 6A) P2X7 expression in type I cells of Caveolin-1-deﬁcient mice was strongly decreased, and reduced immunoreactivity was ... E10 cells After transfection of E10 cells with two different shRNA constructs of Caveolin-1 (shRNA1 and shRNA2) and three different scrambled shRNAs (control 1–3), cells were harvested, and the...

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Báo cáo Y học: RING ﬁnger, B-box, and coiled-coil (RBCC) protein expression in branchial epithelial cells of Japanese eel, Anguilla japonica pot

Ngày tải lên : 17/03/2014, 10:20

... lamella including the chloride cells and pavement cells whose nuclei are labeled by arrows and double arrowheads, respectively, in Fig 9D The nuclei of the pillar cells were not stained with anti-eRBCC ... al [28] and Sasai et al [29] They demonstrated that the chloride cells can be classiﬁed into two types based on the locations in the gill: ﬁlament chloride cells and lamellar chloride cells The ... (C), and Hoechst 33342 (D) A merge of B, C and D is shown in A Arrows point to chloride cells; double arrowheads, pavement cells; and arrowheads, pillar cells Scale bar represents 50 lm Data represent...

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Báo cáo khoa học: Transport of the phosphonodipeptide alafosfalin by the H+/peptide cotransporters PEPT1 and PEPT2 in intestinal and renal epithelial cells doc

Ngày tải lên : 23/03/2014, 12:20

... transepithelial [14C]Gly-Sar flux PEPT1 and PEPT2 are expressed in the apical membranes of intestinal (PEPT1) and renal (PEPT1 or PEPT2) epithelial cells Absorption of intact di- and tri-peptides and ... transepithelial flux and the intracellular accumulation of [14C]Gly-Sar at Caco-2 and SKPT cell monolayers Transepithelial flux and uptake of 10 lM [14C]Gly-Sar from apical to basolateral (Ja-b) and ... incubating the cells for 10, 30, 60 and 120 with alafosfalin at a concentration of mM The recovery rates were 93 ± 0.7% (Caco-2) and 96 ± 0.3% (SKPT) after 10 and 81 ± 3% (Caco-2) and 90 ± 0.8%...

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Báo cáo khoa học: Expression of heme oxygenase-1 is repressed by interferon-c and induced by hypoxia in human retinal pigment epithelial cells pot

Ngày tải lên : 23/03/2014, 13:20

... RPE cells Northern blot analyses of HO-1 mRNA and Bach1 mRNA in human RPE cells treated with the following cytokines: interferon-c (IFNc), TNF-a and IL-1b D407 RPE cells (A) and ARPE-19 cells ... in human RPE cells [23], human renal epithelial cells [56], and A549 human lung cancer cells [57] Thus, TGF-b1 is a candidate that may mediate the hypoxic induction of HO-1 in RPE cells In fact, ... Acknowledgements We thank Dr R.C Hunt for D407 RPE cells and Dr L.M Hjelmeland for ARPE-19 RPE cells We also thank Y Fujii-Kuriyama and E Ito for the HRE constructs and human Bach1 cDNA, respectively This...

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Báo cáo khoa học: Human airway trypsin-like protease induces amphiregulin release through a mechanism involving protease-activated receptor-2-mediated ERK activation and TNF a-converting enzyme activity in airway epithelial cells doc

Ngày tải lên : 30/03/2014, 11:20

... HAT is speciﬁcally found in ciliated epithelial cells; however, it is not found in the basal and goblet cells in the epithelium or in the submucosal gland cells [4] Therefore, it is thought that ... tethered ligand that binds to the receptor and autoactivates it PAR-2 is activated by trypsin and mast cell tryptase and is known to mediate airway inﬂammation both in vitro [7,8] and in vivo ... (cont) and cultured for 72 h (A, C) Cells were then stimulated with HAT (200 nM) for h and ELISA was used to determine the AR concentration in culture supernatant (B) Cells were then collected and...

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báo cáo hóa học:" Retinal pigment epithelial cells secrete neurotrophic factors and synthesize dopamine: possible contribution to therapeutic effects of RPE cell transplantation in Parkinson''''s disease" doc

Ngày tải lên : 18/06/2014, 15:20

... whether RPE cells can produce and release DA Our results indicate that 1) RPE cells can express and secrete GDNF and BDNF and protect DAergic cells against neurotxoins-induced injury; 2) RPE cells ... The studies were designed by MM and WL and were performed by MM, XL, and XF Human RPE cells were separated and cultured by QG DY, LL and SC gave advises on the work and helped in the interpretation ... can express DDC and synthesize DA; 3) RPE cells attached to microcarriers can survive in the host striatum and produce high level GDNF and BDNF after transplantation; and 4) RPE cells transplantation...

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Báo cáo hóa học: " Role of protease-activated receptor-2 on cell death and DNA fragmentation in Helicobacter pylori-infected gastric epithelial cells" ppt

Ngày tải lên : 18/06/2014, 16:20

... death and DNA fragmentation in gastric epithelial cells To investigate the relations of PAR-2 expression, cell death, and DNA fragmentation, cells were transfected with AS ODN for PAR-2 and cultured ... gastric epithelial cells Previously we demonstrated that H pylori induced the expression and activation of PAR-2, which mediates the expression of COX-2 and integrins in gastric epithelial cells ... of various cells including pancreatic and gastric cancer cells, and smooth muscle cells [23-25] Additionally, activation of PAR-2 by agonist or activating peptide protects astrocytes and neutrophils...

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báo cáo hóa học: " Inhibition of gap junctional Intercellular communication in WB-F344 rat liver epithelial cells by triphenyltin chloride through MAPK and PI3-kinase pathways" pdf

Ngày tải lên : 20/06/2014, 00:20

... culture and treatment with chemicals WB-F344 rat liver epithelial cells [43] were cultured in D medium supplemented with 5% fetal bovine serum and 1% [v/v] penicillin/streptomycin antibiotic The cells ... Western blot analysis WB F344 liver cells were treated with TPTC of 1.5 ppm for 15 and 30 After treatment, the medium was removed and cells were washed twice with PBS and lysed with 0.5% SDS Lysates ... P0 (unphosphorylated form), P1 and P2 (phosphorylated forms), respectively [69] After 15-min and 30-min exposure to TPTC, the P0 band disappeared, and a shift to bands of higher molecular weight...

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báo cáo hóa học:" Biocompatibility of Poly-ε-caprolactone-hydroxyapatite composite on mouse bone marrow-derived osteoblasts and endothelial cells" doc

Ngày tải lên : 20/06/2014, 01:20

... these cells seeded on it Figure tion Effects of composite scaffolds on the cells viability and funcEffects of composite scaffolds on the cells viability and function The induced osteoblasts and endothelial ... for cell process and spreading, adhesion and orientation Therefore, osteoblasts and endothelial cells were inclined to attach to the rougher surface and appeared better viability and strong cellular ... (arrows), and cellular projection (1500×) Panels (C) and (D) revealed the growth of endothelial cells on a PCL-HA scaffold The Panel (C) pictured ECs proliferating and forming a cell sheet (600×), and...

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Báo cáo khoa học: "Comparative study of PM2.5 - and PM10 - induced oxidative stress in rat lung epithelial cells" pptx

Ngày tải lên : 07/08/2014, 17:22

... PMs, rat lung epithelial cells were treated with different concentrations of PMs for day, and Comparative study of PM2.5 - and PM10 - induced oxidative stress in rat lung epithelial cells 13 TTGTTTCTCGT-3', ... dishes with 50,000 cells per well They were cultured for 24 h The medium was then replaced After rat lung epithelial cells were treated with various concentrations of PM2.5 and PM10 stock solution, ... collected and removed the biological species such as pollen and endotoxin Collected PMs were weighted and resuspended in PBS solution Resuspension took place in an ultrasonic bath for 30 minutes And...

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báo cáo khoa học: " Salivary a-amylase exhibits antiproliferative effects in primary cell cultures of rat mammary epithelial cells and human breast cancer cells" docx

Ngày tải lên : 10/08/2014, 10:21

... culture than F344 cells Immunocytochemical discrimination between epithelial cells and fibroblasts As the tissue preparation and culture conditions were optimized for epithelial cells, the cell ... F344 cells (P0) Page of 12 b) Lewis cells (P0) 100 μm 100 μm d) Lewis cells (P1) c) F344 cells (P1) 10 μm 10 μm Figure Differences in cultures of primary mammary cells from F344 and Lewis rats and ... control cells treated with water for the total number of cells, exclusively viable, and for dead cells after and 50 U/ml for day, days, and days (n = 4-14 wells per group) For counting, cells were...

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Báo cáo y học: "Inflammatory responses in epithelia: endotoxininduced IL-6 secretion and iNOS/NO production are differentially regulated in mouse mammary epithelial cells" pot

Ngày tải lên : 11/08/2014, 03:20

... myoepithelial cells in maintaining epithelial cell polarity and the bilayered structure of epithelial cells and myoepithelial cells in the normal mammary tissue [33] The ratio of secretory epithelial ... to further understand inflammation and inflammation-associated cell transformation in epithelial cells Methods Cell lines and materials Mouse mammary epithelial cells SCp2 and SCg6 were kindly ... immune cells as well as non-immune cells such as endothelial, fibroblast and epithelial cells, and is often a marker of acute or chronic inflammation in clinical diagnostic assays [10] and found...

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Báo cáo y học: "Early Characterization of Toll-like receptors in primary lung epithelial cells: strong impact of the TLR3 ligand poly(I:C) on the regulation of Toll-like receptors, adaptor proteins and inflammatory response" ppt

Ngày tải lên : 11/08/2014, 08:21

... in pathogen recognition and induction and regulation of the innate and adaptive immune response Airway epithelial cells express TLRs and activation of TLRs on epithelial cells has been shown to ... Figure small airway epithelial cells (SAEC) Cytokine and chemokine secretion by stimulated primary Cytokine and chemokine secretion by stimulated primary small airway epithelial cells (SAEC) SAEC ... metalloproteinase (MMP) and cells secretion Matrix primary small airway epithelial TIMP (SAEC) by stimMatrix metalloproteinase (MMP) and TIMP secretion by stimulated primary small airway epithelial cells (SAEC)...

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Báo cáo y học: "Early Induction of apoptosis with tobacco smoke and related products in A549 lung epithelial cells in vitro" potx

Ngày tải lên : 11/08/2014, 08:21

... minutes at 4°C to recover detached cells Adherent cells were washed with PBS and detached using trypsin EDTA The detached cells were added to the adherent cells and centrifuged The resulting pellet ... nucleotide mix and rTdT enzyme for 60 minutes at 37°C Cells were washed in SCC buffer and stained with DAPI For each treatment 300 cells stained with DAPI (340–380 nm) were randomly counted and assessed ... nicotine (Nic; mM) and hydrogen peroxide (HP; 10 μM) (B) Cells were untreated (control) or treated with 100%, 50% and 25% CSE Closed bars show cells treated for 24 hours and open show cells treated...

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Báo cáo y học: "Early Effects of anti-inflammatory [1, 2, 4]triazolo[4, 3-a] [1, 8]naphthyridine derivatives on human stimulated PMN and endothelial cells: an in vitro study" doc

Ngày tải lên : 11/08/2014, 08:21

... Materials and Methods section (n = 3) Effects of NF161 and NF177 on PGE2 and PGI2 production by pre-induced COX-2 IL-1α (10 ng/ml) was added and incubated for 20 h The cells were washed and NF161 ... contributions CD and MC participated in the study design, carried out the experiments and wrote the manuscript MG performed the ELISA experiments GR and MD designed and synthesized NF161 and NF177 RF ... was 51 ± cells/ microscope fields (n = 30) The direct effect on HUVEC was assessed by pretreated the cells with the 1, 8-naphtyridines (10-4M) and 10-7M PAF for 20 min, washed three times and challenged...

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Báo cáo y học: "Differential cytopathogenesis of respiratory syncytial virus prototypic and clinical isolates in primary pediatric bronchial epithelial cells" pdf

Ngày tải lên : 11/08/2014, 21:22

... BT3a and BT4a PBECs were infected with RSV A2, BT2a, BT3a and BT4a (MOI of 0.1) during h and washed with PBS Seventy two hpi, cells were washed with PBS, fixed with 4% paraformaldehyde and stained ... ethics and institutional governance permission, designed and co-supervised the clinical aspects of the study, and helped with data analyses UFP codesigned, conceived and coordinated the study, and ... culture and growth curves RSV BT2a, BT3a, BT4a and RSV A2 were cultured in HEp-2 cells as previously described [11], except that DMEM was the medium of choice To minimize adaptation to HEp-2 cells, ...

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Báo cáo y học: " Profiles of cytokine and chemokine gene expression in human pulmonary epithelial cells induced by human and avian influenza viruses" doc

Ngày tải lên : 12/08/2014, 02:20

... monocytes, eosinophils, basophils, and CD4+ T cells [45] CCL-5/RANTES production from bronchial epithelial cells contributes to infiltration of inflammatory cells in the airway during viral infection ... proliferation and differentiation of B cells, and proliferation of T cells As a result, all these TNF-a activated mediators could contribute to the infiltration of inflammatory cells into the ... cytokine and chemokine gene expression in human pulmonary epithelial cells induced by human and avian influenza viruses Virology Journal 2010 7:344 Submit your next manuscript to BioMed Central and...

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