feasibility and safety in a porcine model with short time survival

... additional untreated healthy animals were sacrificed Premedication and anesthesia The animals were premedicated with ketamine and azaperon Pigs were anesthetized with analgosedation (Thiopental), ... -F: 5'-CAGCCCAACAGCATGCCCCAG-3', Genosys-Sigma, Munich, Germany) Primers for glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (hGAPDH-R: 5'-GAAGTCAGAGGAGACCACCA-3'; -F: 5'-CACCACCATGGAGAAGGCTG-3', ... T, Yamamoto H, Tabata T, Shimizu T, Endo Y, Hanasawa K, Fujimiya M, Tani T: A free radical scavenger, edaravone (MCI-186), diminishes intestinal neutrophil lipid peroxidation and bacterial translocation...

Ngày tải lên: 11/08/2014, 03:20

... vacuolated, and mitochondria swelled to a circular shape with a clear matrix and short or disappeared cristae, which were vacuolar appearances Smooth and rough endoplasmic reticulum expanded and ... ear veins and ketamine was infused (50 mg/h) for anesthesia Diazepam was administered as needed The HIFU ablation procedure complies with the guidance of the National Standard of China and was described ... Because HIFU is minimally invasive and accurate, and possesses real -time targeting, it provides patients with a new therapeutic option with less pain and damage to the splanchnic functions and...

Ngày tải lên: 25/10/2012, 11:18

... pronounced decrease in MAC-1 in animals immunized with oligo A or fAβ as compared to untreated or adjuvant treated transgenic mice (Figure 5A) Mean % area of MAC-1 staining in oligo A and fAβ immunized ... of animals at 12–16 months with MAPAβ1–33 decreases thioflavine and anti CD45 reactivity Immunization of animals at 12–16 months with MAPAβ1–33 decreases thioflavine and anti CD45 reactivity A ... In contrast, no significant decrease was seen in any pathological marker measured in mice vaccinated with any peptide form when vaccination was initiated at 16 months, a time at which there are...

Ngày tải lên: 19/06/2014, 22:20

... sretsmah ni egamad AND evitartin inirreviv sihcrohtsipO dna evitadixo detaidem-ON fo msinahceM S ihsinawaK ,P nrowahtihtiS ,B apirS ,M ataruM ,S awakiO ,R abmeS ,P tinavgnoY ,N aM ,Y ukariH ,S roalniP ... ,doirep latnemirepxe eritne eht tuohguorht mutibil da retaw pat gniknird dna )aeroK ,gnaymaS( teid lamron a nevig erew slaminA aeroK ni ytisrevinU lanoitaN nowgnaK ta ytilicaf lamina devorppa na ni ... yliäS ,R kiiW-ohaneetraaK ,U sagnaknäpäN ,K solhaK ,Y inioS 52 4276 -6176 ,46 ,4002 seR recnaC sserts evitadixo ecudni taht stnega recnacitna rof tegrat ralucelom laitnetop a sa esatcuder nixoderoihT...

Ngày tải lên: 07/08/2014, 18:21

... for hematoxylin and eosin staining and immunostaining Pieces of the left lung were fixed in 2% paraformaldehyde containing 0.1% glutaraldehyde for hours at 4°C and processed for embedding in LR ... investigate the role of PIMs in endotoxin-induced mortality and lung inflammation The data show that PIM depletion as well as inactivation prevents mortality and lung inflammation in LPS-treated ... LPS-induced lung inflammation and mortality in BDL rats and GCtreated BDL rats The dramatic finding was 100% and 80% survival in BDL rats that were treated with GC 48 hours and hours, respectively,...

Ngày tải lên: 12/08/2014, 14:20

... separate pens with 3–10 animals in each pen, using straw as beddings After weaning, the animals were given hay and grass silage ad libitum, and 0.5–2 kg grain per day increasing with age Breeding ... improve both health and weight gain [7,8] Separating calves and cows days after birth has been associated with health advantages for the calf, compared to separation at birth [9] Calves allowed to ... not for citation purposes) Acta Veterinaria Scandinavica 2007, 49:16 erinarians in Norway, thus health recordings are viewed as reliable The yearly health situation for all individuals in the present...

Ngày tải lên: 12/08/2014, 18:22

... killed as described above Statistical analysis Values are expressed as mean ± standard deviation The comparability of baseline data was verified using one-way analysis of variance Survival rates ... end-expiratory pressure, and tidal volume adjusted to maintain normocapnia Anaesthesia was maintained with propofol (6 to mg/kg per hour) and a single injection of piritramide (30 mg) [11] Muscle paralysis ... differences in study end-points Materials and methods Surgical preparations and measurements The project was approved by the Austrian Federal Animal Investigational Committee, and the animals were managed...

Ngày tải lên: 13/08/2014, 08:20

... ω  ≡ UlL ω2 ω (9) NEUTRINO MASSES AND MIXING IN THE STANDARD MODEL WITH 257 II.3 Neutrino sector To obtain arbitrary Majorana neutrino masses, four Higgs doublets are used: σ= + σ11 σ12 + ... THE STANDARD MODEL WITH V = µ0 φT σφ + µ1 φT s1 φ 259 (29) III CONCLUSION We have shown the neutrinos gain naturely small masses in agreement with experiment The neutrino mixing matrix in terms ... −1/√  (19) −1/ 1/ 1/ This is a main result of the paper 258 NGO THI THU DINH, PHUNG VAN DONG II.4 Scalar Potential We can separate the general scalar potential into V = V φ + V σs , (20) where...

Ngày tải lên: 30/10/2015, 19:44

... the treated animals to measure the biochemistry markers including alanine transaminase (ALT), aspartate aminotransferase (AST), blood urea nitrogen (BUN) and creatine (Cr) using commercial kits ... transferasemediated dUTP nick-end labeling; ALT: alanine transaminase; AST: aspartate aminotransferase; BUN: blood urea nitrogen; Cr: Creatinine Acknowledgements This work was supported by grants ... and in vivo, by determining the tumor size and animal survival, assessing both tumoral histology and infiltration of Page of 10 T-lymphocytes, and evaluating toxic studies The data showed that...

Ngày tải lên: 18/06/2014, 19:20

... the treated animals to measure the biochemistry markers including alanine transaminase (ALT), aspartate aminotransferase (AST), blood urea nitrogen (BUN) and creatine (Cr) using commercial kits ... transferasemediated dUTP nick-end labeling; ALT: alanine transaminase; AST: aspartate aminotransferase; BUN: blood urea nitrogen; Cr: Creatinine Acknowledgements This work was supported by grants ... and in vivo, by determining the tumor size and animal survival, assessing both tumoral histology and infiltration of Page of 10 T-lymphocytes, and evaluating toxic studies The data showed that...

Ngày tải lên: 20/06/2014, 03:20

... temperature and arterial and central venous pressure by femorally introduced catheters connected to a standard anaesthesia monitor (AS/3, Datex Ohmeda, Helsinki, Finland) Surgical preparation and ... were used at a concentration of 1:100 For staining, the ABC Vectastain universal kit (Vector Laboratories, Burlingame, CA, USA) and haematoxylin as counterstain was used A blinded pathologist ... measurements and coagulation parameters after haemodilution Baseline parameters were comparable between groups (Tables and 2) The dilution caused a significant coagulopathy and a drop in platelets PT increased...

Ngày tải lên: 13/08/2014, 20:21

... study and participated in its design, coordination and statistical analysis and helped to draft the manuscript All the authors have read and approved the final manuscript Competing interests The authors ... carcass composition and intramuscular fat content (IMF), the season/year of birth and the sex were included in the model as fixed effects (F) and carcass weight and age at slaughter as covariates ... Noguera JL, Perez-Enciso M, Coll A, Folch JM, Barragan C, Toro MA, Babot D, Sanchez A: QTL mapping for growth and carcass traits in an Iberian by Landrace pig intercross: additive, dominant and...

Ngày tải lên: 14/08/2014, 13:21

... attachment to surfaces, structural stability and spatial arrangement of biofilm structure and serve as a protective barrier against desiccation and retards access of harmful substances such as antibiotics ... serve as a protective barrier against dehydration and enhance resistance of the biofilm to harmful substances such as antimicrobial agents, and bacteriophage The mechanism works in such a way that ... et al., 1999) The biofilms in DWDS are always under starvation and achieve a stationary phase growth and more veteran survivals are available after the initial disinfection under the EPS protection...

Ngày tải lên: 11/09/2015, 09:17

... with reasonable accuracy In a recent study, Cho et al [12] investigated the effect of a gap in the inclined rib on heat transfer in a square duct and reported that a gap in the inclined rib accelerates ... heat transfer and flow behavior in a rectangular rib roughened passage” International Journal of Heat and mass transfer; 123: 675-681, 2001 [6] Lau S.C., McMillin R.D and Han J.C Turbulent Heat ... 2057-2073 [18] Momin A. M.E., Saini J.S and Solanki S.C Heat transfer and friction in solar air heater duct with V-shaped rib roughness on absorber plate Int Journal of Heat and Mass Transfer 2002;...

Ngày tải lên: 05/09/2013, 16:10

... mitochondrial Peroxiredoxin Annexin A5 Annexin A2 Aldolase A Fascin Pyruvate kinase VDAC-2 Stathmin Ran1BP GSTp C1qBP Profilin Enolase RuvB-like CRMP4 Lamin A ⁄ C Mitofilin, p32 GAPDH ATP synthase a P05387 ... acidic ribosomal protein P2 (RPLP2), eukaryotic initiation factor 5A (eIF 5A) , parathymosin, L7 ⁄ L12, annexin A2 , annexin A5 , aldolase A, fascin and peroxyredoxin 1] displayed quantitative differences, ... (Stratagene, Santa Clara, CA, USA) (150 ngÆwell)1) and phRL-CMV, containing Renilla luciferase cDNA (5 ngÆwell)1), using Lipofectamine and OptiMEM medium (Invitrogen, Carlsbad, CA, USA) In pNF-jB-Luc...

Ngày tải lên: 15/02/2014, 01:20

... Peroxiredoxin Apolipoprotein A- I Abhydrolase domain containing 14b D-Amino Protein name ApoA4 ActB Nsfl1C Acy1 Pgam1 Inmt Prdx6 ApoA1 Abhd14b DnaJA1 Pdzk1 Hao3 Erp29 Aco1 Me1 Eno1 Dao1 Hao3 Mpst Acads Short ... [8] indicate that its alanineglyoxylate aminotransferase activity is not favored over aminobutyrate-pyruvate, b-alanine-pyruvate and dimethylarginine-pyruvate aminotransferase activities In the ... Hernandez-Fernaud and E Salido Proteome changes in primary hyperoxaluria and males develop calcium oxalate crystalluria and calculi in the urine bladder, although no deposits in the renal parenchyma...

Ngày tải lên: 23/03/2014, 03:20

... hydroxyl-radical scavenging and a novel, glutathione-sparing mechanism of action Arch Biochem Biophys 2000, 381:253-263 de la Lastra CA, Villegas I: Resveratrol as an anti-inflammatory and anti-aging agent: ... Sclafani RA, Agarwal R: Resveratrol causes Cdc2-tyr15 phosphorylation via ATM/ ATR-Chk1/2-Cdc25C pathway as a central mechanism for S phase arrest in human ovarian carcinoma Ovcar-3 cells Carcinogenesis ... and Topoisomerase-alpha antibody Primary antibody staining was followed by incubation with antimouse or anti-rabbit secondary IgG polymer conjugated with HRP or Alkaline phosphatase and signals...

Ngày tải lên: 18/06/2014, 15:20

... UK and US weights for all known groups including HbA1c level (above and below 7%) and diabetic complications (presence and absence) namely neuropathy, retinopathy, nephropathy and cardiovascular ... Sintonen H, Tsuchiya A, Williams A, Yfantopoulos J, De Charro F: A comparison of EQ-5D time trade-off values obtained in Germany, United Kingdom, Spain In The measurement and valuation of health ... analyzing and collecting the data All authors have read and approved the final manuscript Acknowledgements This research was supported by a grant from Chulalongkorn University The authors thank diabetic...

Ngày tải lên: 18/06/2014, 19:20

... IFN-g release and cell-mediated cytotoxicity after vaccination with mGC8 cells and GM-CSF T cells generated from TVDLN at day nine after vaccination were polyclonally activated and expanded as described ... D: A phase II study of G17DT in gastric carcinoma Eur J Surg Oncol 2004, 30:536-543 Sato Y, Fujiwara T, Mine T, Shomura H, Homma S, Maeda Y, Tokunaga N, Ikeda Y, Ishihara Y, Yamada A, Tanaka N, ... injected days before vaccination and tumor vaccinations were repeated every two weeks for a total of vaccinations In vitro T cell activation and expansion For T cell analyses, mice were vaccinated...

Ngày tải lên: 18/06/2014, 22:20