... their positions. The arrowheads depict the residues important for the structural core ofthe IL-10 gene. The underlined amino acid residues are the signal sequences ofthe respective genes. The ... analysis investi-gated. This work will pave the way for further investigation of the biological function of this gene, and the probability of the presence of IL-10-related genes in fish, as seen inmammals.AcknowledgementsThis ... against products of b-actin. The x-axis indicates the time-periods of LPS incubation and the relative expression of the carp IL-10 gene on the y-axis. Controls for 0, 1, 3 and 6 h of incubation...
... 1).Subsite mappingOn the basis ofthe assumptions of Hiromi [30] that the intrinsic rate of hydrolysis (kint) in the productivecomplex is independent ofthe length ofthe substrate,Kmand ... Laboratory of Microbiology, Wageningen University, the Netherlands2 Laboratory of Food Chemistry, Wageningen University, the NetherlandsPectin is a complex polysaccharide present in the cellwall of ... (1973) Subsiteaffinities of glucoamylase: examination ofthe validity of the subsite theory. Biochim Biophys Acta 302, 362–375.L. Kluskens et al. An exopolygalacturonase from Thermotoga maritimaFEBS...
... (e.g., the height of a person), whilefor others it is only the center of a distribution(e.g., the weight of a coffee cup). Questionswith no trusted and exact answers were eliminated. From the ... information for the requested object, the requested object will receive the average ofthe extracted values ofthe whole set of the retrieved comparable objects and the com-parison step will be essentially ... value for the givenobject. During the first stage it is possible thatwe directly extract fromthe text a set of valuesfor the requested object. The bounds processingstep rejects some of these...
... becollected from each seed word, and then checkedwhether each ofthe target terms was included in the system output. We counted the number of tar-get terms in the following five cases. The right ... total; the average number of output terms per input is 12.2terms. We checked whether each ofthe 610 termsis a correct related term ofthe original seed term byhand. The result is shown in the ... term.3. There is a definition or explanation ofthe term.4. There are several technical terms that are re-lated to the term.We have implemented the checking program of the first two conditions in the...
... quality model to estimate the dispersion function, the accuracy ofthe estimated emissions will depend on the ability ofthe model to reproduce the dispersion ofthe pollutants. Until now, ... observed at this period ofthe day. From 2 pm to 6 pm, the wind direction is oblique to the street axis and the wind speed is higher than in the morning. At these hours ofthe day, tracer ... which, C is the concentration of a particular pollutant in the street (g/m3 or mg/m3); E is the emission ofthe pollutant from road traffic in the street; F is a function describing the dispersion,...
... the finding that at pH 10 and 75 °C, the inactivation of b-glucosidase also does not involveany loss of secondary structure [1].Conclusions The stability ofthe b-glucosidase fromthe hyperther-mophile ... 113 ofthe large volume change associated with the forma-tion of a free charge [30]. Hence, a reduction in the number of electrostatic interactions would increase the pressure stability ofthe ... addi-tion, the band at 1.0 GPa in Fig. 5C does notresemble the broad, featureless band typical of anunfolded protein. Taken together, these observationssuggest that the unfolding at the level of the...
... arrows represent the direction of transcription; the site ofthe transposon insertion identified in the O35E is denotedas EN:TN, and the location ofthe deletion replaced by the kanamy-cin-resistant ... moieties. The ions with m ⁄ z of 1519.6and 1541.6 are mono- and di-sodiated forms of the de-O-acylated LOS and the ion with m ⁄ z of 1479.6 is the anhydrous form ofthe de-O-acylated LOS. The ion ... de-O-acylated LOSs fromthe wild-type O35Eand the mutant O35Elgt3 were further analyzed byMALDI-TOF MS (Fig. 4). The mass spectral data of de-O-acylated LOS from O35E was consistent with the reported...
... construct expected pay from civilian airlines. Furthermore,because much ofthe variation ofthe civilian pay profile lies in the probability of being hired, the number of civilian major airline ... characteristics of militaryemployment, such as the DOD occupation code). Appended to the accession datais information about the officer at the time of separation if the officer is no longerin the service, ... Specification ofthe Pilot Attrition ModelIn this section, we discuss the details ofthe development ofthe specificmathematical form of our pilot attrition model.Dependent Variable The dependent...
... positively affected the activity andstability ofthe enzyme [40]. The effect of copper onenzyme stability may be related to the inhibitory effect of the metal on the activity of an extracellular ... to analyse the expression ofthe gene.Right panel: PCR amplification ofthe clonedlac1 cDNA using the cycle number obtained from the left panel. Each value represents the mean ± SD of three PCR ... as above. The full-length cDNA of lac1 was then generated by 3Â-RACEusing the primer 5Â-TCTCAACCGTCGACAGCAGTGTTCGTG-3Â designed fromthe sequence ofthe extreme5Â end of lac1. The full-length...
... SGTIis the differing conformation of their binding loops. The corresponding NOE pattern of SGTI is different from that of SGCI as well as from that recorded f or SGCI[L30R,K31M]. The number of observed ... inhibitors.In the light ofthe solution structures reported here, weattempt to address the question of taxon-speciđc inhibitoryaction fromthe inhibitor side. The binding loops of each of the molecules ... degree of stabilization by interactions with the core ofthe molecule.Although w e only have i ndirect evidence concerning the ¯exibility ofthe binding loop ofthe inhibitors, we suggestthat the...
... and indeed, the site-specific mutagenesis of K253 to Met led to an inactive protein [23]. In addition, the alkylation ofthe adjacent M258 inactivated the IDH from A. vinelandii [26]. These Lys ... that leads to inactivation of the enzyme and consequent switch ofthe carbon flux from TCAcycle to glyoxylate cycle [13] [14].There are two k inds of IDH with different cofactordependency, NAD- ... ATP-citrate lyase[24]. The results demonstrated the heteromeric structure o fthis enzyme and its role in regulating the d irection and flux of the RTCA cycle. For further understanding ofthe R TCAcycle,...
... dismutase. Furthermore, we investigated the binding of hydroxide and imidazole to the ferric enzyme and of NO to the ferrous enzyme to establish the nature of the proximal ligand bound to the heme ... the enzyme from GR-1 [4]. However, the existence of a second high-spin species, which we have designated the ÔbroadÕ signal, has not been reported previously. The nature of the multiplicity of ... for the formation of acomplex with hydrogen peroxide (Fig. 1, trace E). The EPRspectrum ofthe hydrogen peroxide complex, however,shows a decrease in the high-spin signal ofthe enzyme andthe...
... pKa2. The pKavalue is deter-mined from a fit ofthe phosphorus chemical shifts of the without ionic, monoionic and diionic forms of the phosphate group as a function of pH [33,34]. The phosphorus ... affects the electrostatic potential andquite often the conformation ofthe modified protein.Even in the absence of rearrangement, the changein the electric field and steric hindrance from aphosphate ... contribution ofthe R4 repeat to PHFformation remains to be elucidated, even though the roles ofthe R2 and R3 repeats in the aggregation of tauhave been reported [22]. Moreover, there is no...
... name”) The value ofthe :cs feature is the constituent struc-ture ofthe subcategorisation frame, which lists the syntactic CF-PSG constituents in sequence. The value ofthe :gs feature is the ... lexicon extracted from Sec-tion 23 ofthe WSJ. It was found that the referenceCCG lexicon contained 95.09% ofthe entries in the test lexicon, while 94.03% ofthe entries in the testTAG lexicon ... fea-ture to the generated f-structures, the value of whichis the syntactic category ofthe pred at each level of embedding. Exploiting this information, the ex-tracted semantic form for the verb...
... in the amino-acid sequence of P. hilaris cellulase. Alternatively, the mobility of the P. hilaris cellulase protein on SDS/PAGE may be different from those ofthe standard proteins used in the ... to belong to GHF 5. The activity staining indicated that there were no other CMCaseactivities except the GHF 5 cellulase in the larval gut of P. hilaris. The sensitivity ofthe activity staining ... found in the sequence(Fig. 3). Genomic PCR experiments with a variety of different primers designed fromthe cDNA sequenceresulted in the amplification of bands in each case.Sequencing of these...