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Tài liệu Báo cáo khoa học: Cloning, characterization and expression analysis of interleukin-10 from the common carp, Cyprinus carpio L. docx

Tài liệu Báo cáo khoa học: Cloning, characterization and expression analysis of interleukin-10 from the common carp, Cyprinus carpio L. docx

... their positions. The arrowheads depict the residues important for the structural core of the IL-10 gene. The underlined amino acid residues are the signal sequences of the respective genes. The ... analysis investi- gated. This work will pave the way for further investigation of the biological function of this gene, and the probability of the presence of IL-10-related genes in fish, as seen in mammals. Acknowledgements This ... against products of b-actin. The x-axis indicates the time-periods of LPS incubation and the relative expression of the carp IL-10 gene on the y-axis. Controls for 0, 1, 3 and 6 h of incubation...

Ngày tải lên: 20/02/2014, 02:21

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Tài liệu Báo cáo khoa học: Characterization and mode of action of an exopolygalacturonase from the hyperthermophilic bacterium Thermotoga maritima doc

Tài liệu Báo cáo khoa học: Characterization and mode of action of an exopolygalacturonase from the hyperthermophilic bacterium Thermotoga maritima doc

... 1). Subsite mapping On the basis of the assumptions of Hiromi [30] that the intrinsic rate of hydrolysis (k int ) in the productive complex is independent of the length of the substrate, K m and ... Laboratory of Microbiology, Wageningen University, the Netherlands 2 Laboratory of Food Chemistry, Wageningen University, the Netherlands Pectin is a complex polysaccharide present in the cell wall of ... (1973) Subsite affinities of glucoamylase: examination of the validity of the subsite theory. Biochim Biophys Acta 302, 362–375. L. Kluskens et al. An exopolygalacturonase from Thermotoga maritima FEBS...

Ngày tải lên: 20/02/2014, 03:20

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Tài liệu Báo cáo khoa học: "Extraction and Approximation of Numerical Attributes from the Web" pdf

Tài liệu Báo cáo khoa học: "Extraction and Approximation of Numerical Attributes from the Web" pdf

... (e.g., the height of a person), while for others it is only the center of a distribution (e.g., the weight of a coffee cup). Questions with no trusted and exact answers were eliminated. From the ... information for the requested object, the requested object will receive the average of the extracted values of the whole set of the retrieved comparable objects and the com- parison step will be essentially ... value for the given object. During the first stage it is possible that we directly extract from the text a set of values for the requested object. The bounds processing step rejects some of these...

Ngày tải lên: 20/02/2014, 04:20

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Tài liệu Báo cáo khoa học: "Automatic Collection of Related Terms from the Web" pptx

Tài liệu Báo cáo khoa học: "Automatic Collection of Related Terms from the Web" pptx

... be collected from each seed word, and then checked whether each of the target terms was included in the system output. We counted the number of tar- get terms in the following five cases. The right ... total; the average number of output terms per input is 12.2 terms. We checked whether each of the 610 terms is a correct related term of the original seed term by hand. The result is shown in the ... term. 3. There is a definition or explanation of the term. 4. There are several technical terms that are re- lated to the term. We have implemented the checking program of the first two conditions in the...

Ngày tải lên: 20/02/2014, 16:20

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Báo cáo " Estimation of emission factors of air pollutants from the road traffic in Ho Chi Minh City " docx

Báo cáo " Estimation of emission factors of air pollutants from the road traffic in Ho Chi Minh City " docx

... quality model to estimate the dispersion function, the accuracy of the estimated emissions will depend on the ability of the model to reproduce the dispersion of the pollutants. Until now, ... observed at this period of the day. From 2 pm to 6 pm, the wind direction is oblique to the street axis and the wind speed is higher than in the morning. At these hours of the day, tracer ... which, C is the concentration of a particular pollutant in the street (g/m 3 or mg/m 3 ); E is the emission of the pollutant from road traffic in the street; F is a function describing the dispersion,...

Ngày tải lên: 05/03/2014, 16:20

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Báo cáo khoa học: Increased susceptibility of b-glucosidase from the hyperthermophile Pyrococcus furiosus to thermal inactivation at higher pressures pptx

Báo cáo khoa học: Increased susceptibility of b-glucosidase from the hyperthermophile Pyrococcus furiosus to thermal inactivation at higher pressures pptx

... the finding that at pH 10 and 75 °C, the inactivation of b-glucosidase also does not involve any loss of secondary structure [1]. Conclusions The stability of the b-glucosidase from the hyperther- mophile ... 113 of the large volume change associated with the forma- tion of a free charge [30]. Hence, a reduction in the number of electrostatic interactions would increase the pressure stability of the ... addi- tion, the band at  1.0 GPa in Fig. 5C does not resemble the broad, featureless band typical of an unfolded protein. Taken together, these observations suggest that the unfolding at the level of the...

Ngày tải lên: 07/03/2014, 03:20

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Báo cáo khoa học: Role of different moieties from the lipooligosaccharide molecule in biological activities of the Moraxella catarrhalis outer membrane pot

Báo cáo khoa học: Role of different moieties from the lipooligosaccharide molecule in biological activities of the Moraxella catarrhalis outer membrane pot

... arrows represent the direction of transcription; the site of the transposon insertion identified in the O35E is denoted as EN:TN, and the location of the deletion replaced by the kanamy- cin-resistant ... moieties. The ions with m ⁄ z of 1519.6 and 1541.6 are mono- and di-sodiated forms of the de-O-acylated LOS and the ion with m ⁄ z of 1479.6 is the anhydrous form of the de-O-acylated LOS. The ion ... de-O-acylated LOSs from the wild-type O35E and the mutant O35Elgt3 were further analyzed by MALDI-TOF MS (Fig. 4). The mass spectral data of de-O-acylated LOS from O35E was consistent with the reported...

Ngày tải lên: 07/03/2014, 05:20

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Modeling the Departure of Military Pilots from the Services ppt

Modeling the Departure of Military Pilots from the Services ppt

... construct expected pay from civilian airlines. Furthermore, because much of the variation of the civilian pay profile lies in the probability of being hired, the number of civilian major airline ... characteristics of military employment, such as the DOD occupation code). Appended to the accession data is information about the officer at the time of separation if the officer is no longer in the service, ... Specification of the Pilot Attrition Model In this section, we discuss the details of the development of the specific mathematical form of our pilot attrition model. Dependent Variable The dependent...

Ngày tải lên: 07/03/2014, 05:20

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Báo cáo khóa học: Biochemical and molecular characterization of a laccase from the edible straw mushroom, Volvariella volvacea docx

Báo cáo khóa học: Biochemical and molecular characterization of a laccase from the edible straw mushroom, Volvariella volvacea docx

... positively affected the activity and stability of the enzyme [40]. The effect of copper on enzyme stability may be related to the inhibitory effect of the metal on the activity of an extracellular ... to analyse the expression of the gene. Right panel: PCR amplification of the cloned lac1 cDNA using the cycle number obtained from the left panel. Each value represents the mean ± SD of three PCR ... as above. The full- length cDNA of lac1 was then generated by 3Â-RACE using the primer 5Â-TCTCAACCGTCGACAGCAG TGTTCGTG-3Â designed from the sequence of the extreme 5Â end of lac1. The full-length...

Ngày tải lên: 07/03/2014, 15:20

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Báo cáo khoa học: Comparative structure analysis of proteinase inhibitors from the desert locust, Schistocerca gregaria pptx

Báo cáo khoa học: Comparative structure analysis of proteinase inhibitors from the desert locust, Schistocerca gregaria pptx

... SGTI is the differing conformation of their binding loops. The corresponding NOE pattern of SGTI is different from that of SGCI as well as from that recorded f or SGCI[L30R, K31M]. The number of observed ... inhibitors. In the light of the solution structures reported here, we attempt to address the question of taxon-speciđc inhibitory action from the inhibitor side. The binding loops of each of the molecules ... degree of stabilization by interactions with the core of the molecule. Although w e only have i ndirect evidence concerning the ¯exibility of the binding loop of the inhibitors, we suggest that the...

Ngày tải lên: 08/03/2014, 10:20

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Báo cáo khoa học: Characterization of isocitrate dehydrogenase from the green sulfur bacterium Chlorobium limicola A carbon dioxide-fixing enzyme in the reductive tricarboxylic acid cycle ppt

Báo cáo khoa học: Characterization of isocitrate dehydrogenase from the green sulfur bacterium Chlorobium limicola A carbon dioxide-fixing enzyme in the reductive tricarboxylic acid cycle ppt

... and indeed, the site-specific mutagenesis of K253 to Met led to an inactive protein [23]. In addition, the alkylation of the adjacent M258 inactivated the IDH from A. vinelandii [26]. These Lys ... that leads to inactivation of the enzyme and consequent switch of the carbon flux from TCA cycle to glyoxylate cycle [13] [14]. There are two k inds of IDH with different cofactor dependency, NAD- ... ATP-citrate lyase [24]. The results demonstrated the heteromeric structure o f this enzyme and its role in regulating the d irection and flux of the RTCA cycle. For further understanding of the R TCA cycle,...

Ngày tải lên: 08/03/2014, 10:20

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Báo cáo Y học: Spectroscopic characterization and ligand-binding properties of chlorite dismutase from the chlorate respiring bacterial strain GR-1 ppt

Báo cáo Y học: Spectroscopic characterization and ligand-binding properties of chlorite dismutase from the chlorate respiring bacterial strain GR-1 ppt

... dismutase. Furthermore, we investigated the binding of hydroxide and imidazole to the ferric enzyme and of NO to the ferrous enzyme to establish the nature of the proximal ligand bound to the heme ... the enzyme from GR-1 [4]. However, the existence of a second high-spin species, which we have designated the ÔbroadÕ signal, has not been reported previously. The nature of the multiplicity of ... for the formation of a complex with hydrogen peroxide (Fig. 1, trace E). The EPR spectrum of the hydrogen peroxide complex, however, shows a decrease in the high-spin signal of the enzyme and the...

Ngày tải lên: 08/03/2014, 16:20

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Báo cáo khoa học: Phosphorylation modulates the local conformation and self-aggregation ability of a peptide from the fourth tau microtubule-binding repeat pdf

Báo cáo khoa học: Phosphorylation modulates the local conformation and self-aggregation ability of a peptide from the fourth tau microtubule-binding repeat pdf

... pK a2 . The pK a value is deter- mined from a fit of the phosphorus chemical shifts of the without ionic, monoionic and diionic forms of the phosphate group as a function of pH [33,34]. The phosphorus ... affects the electrostatic potential and quite often the conformation of the modified protein. Even in the absence of rearrangement, the change in the electric field and steric hindrance from a phosphate ... contribution of the R4 repeat to PHF formation remains to be elucidated, even though the roles of the R2 and R3 repeats in the aggregation of tau have been reported [22]. Moreover, there is no...

Ngày tải lên: 16/03/2014, 05:20

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Báo cáo khoa học: "Large-Scale Induction and Evaluation of Lexical Resources from the Penn-II Treebank" potx

Báo cáo khoa học: "Large-Scale Induction and Evaluation of Lexical Resources from the Penn-II Treebank" potx

... name”) The value of the :cs feature is the constituent struc- ture of the subcategorisation frame, which lists the syntactic CF-PSG constituents in sequence. The value of the :gs feature is the ... lexicon extracted from Sec- tion 23 of the WSJ. It was found that the reference CCG lexicon contained 95.09% of the entries in the test lexicon, while 94.03% of the entries in the test TAG lexicon ... fea- ture to the generated f-structures, the value of which is the syntactic category of the pred at each level of embedding. Exploiting this information, the ex- tracted semantic form for the verb...

Ngày tải lên: 17/03/2014, 06:20

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Báo cáo khoa học: Purification, characterization, cDNA cloning and nucleotide sequencing of a cellulase from the yellow-spotted longicorn beetle, Psacothea hilaris ppt

Báo cáo khoa học: Purification, characterization, cDNA cloning and nucleotide sequencing of a cellulase from the yellow-spotted longicorn beetle, Psacothea hilaris ppt

... in the amino-acid sequence of P. hilaris cellulase. Alternatively, the mobility of the P. hilaris cellulase protein on SDS/PAGE may be different from those of the standard proteins used in the ... to belong to GHF 5. The activity staining indicated that there were no other CMCase activities except the GHF 5 cellulase in the larval gut of P. hilaris. The sensitivity of the activity staining ... found in the sequence (Fig. 3). Genomic PCR experiments with a variety of different primers designed from the cDNA sequence resulted in the amplification of bands in each case. Sequencing of these...

Ngày tải lên: 17/03/2014, 10:20

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