fe s protein assembly cia machinery
Thí nghiệm Fe + S
... S N Fe FeS FeS Fe S S FeFeS FeFeS S Fe Fe Fe SFe FeS FeS FeS FeS Fe FeS S FeS FeS Fe ...
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Báo cáo khoa học: Prevalence of intrinsic disorder in the hepatitis C virus ARFP/Core+1/S protein doc
... often associated with disordered proteins [33] Self -assembly of HCV Core+1 /S proteins DLS allows the oligomeric status of proteins in solution to be evaluated Hydrodynamic radius distributions were ... Core+1 /S remains soluble after heat pulse treatment, as observed on fluorescence spectra Moreover, DLS shows that the mass distribution shifts to lower molecular masses This is confirmed by the observation ... However, it was surprising to observe MBP fusion proteins in the insoluble fractions, as the MBP carrier is also a well-known protein solubilizer Despite its small size, Core+1 /S seems, therefore,...
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Báo cáo khoa học: The role of the Fe-S cluster in the sensory domain of nitrogenase transcriptional activator VnfA from Azotobacter vinelandii potx
... involvement of [Fe3 S4 ]+ in VnfA) However, the EPR data cannot exclude possible presence of other types of Fe- S clusters, such as 4Fe- 4S ( [Fe4 S4 ]) and 2Fe- 2S ( [Fe2 S2 ]), because the Fe- S clusters could ... between Cys8 and Cys10 is unusual in ligands for a single Fe- S cluster, it is unlikely that all the N-terminal cysteine residues in the subunit of VnfA bind the single Fe- S cluster A possible scenario ... sequence, Cys8Glu9Cys10, restricts the cysteine residues from binding to the same cluster Discussion Prosthetic group of VnfA The EPR data of the whole E coli cells overexpressing vnfA suggested...
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Báo cáo khoa học: Frataxin deficiency causes upregulation of mitochondrial Lon and ClpP proteases and severe loss of mitochondrial Fe–S proteins pot
... change in Fe S protein levels was observed, suggesting a defect in Fe S cluster assembly The decrease in mitochondrial Fe S cluster proteins was clearly apparent at weeks ( 40–70% decrease) in ... contains Fe S clusters or is related to Fe S proteins, was unaffected in mutant mice, indicating that frataxin deficiency specifically affects Fe S proteins and their protein partners These results ... component for Fe S cluster biogenesis, decreases the abundance of several Fe S proteins and partners [58] By statistical analysis, we found that the time course of mitochondrial Fe S protein loss in MCK...
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Báo cáo khóa học: Protein assembly of photosystem II and accumulation of subcomplexes in the absence of low molecular mass subunits PsbL and PsbJ pdf
... numerous studies with Synechocystis 6803 mutants of the LMM subunits of PSII has addressed the PSII assembly process as such, but instead the focus has been on functional properties of PSII and ... remains to be resolved PsbL is required for stable assembly of CP43 Comparison of the assembly of PSII in DpsbL and DpsbJ clearly demonstrates that PsbL is essential at earlier assembly steps than ... understanding the assembly of LMM subunits into PSII is to use specific PSII protein deletion mutants and to analyse the ability of such mutants to form various PSII subassemblies This approach has...
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Báo cáo y học: "Parvovirus B19 Genotype Specific Amino Acid Substitution in NS1 Reduces the Protein’s Cytotoxicity in Culture"
... http://www.medsci.org Int J Med Sci 2010, Statistical Analysis Student t-test was used to assess the statistical significance in the data RESULTS Substitution I181M in B19 NS1 interferes with NS1-induced ... undergoing apoptosis We used the AnnexinV binding assay to assess apoptotic cell death in HepG2 cells The pattern of apoptosis observed in HepG2 cells expressing all B19 NS1 constructs showed continually ... analysis of adeno-associated virus type Rep68 protein endonuclease activity on partially single-stranded substrates J Virol 2000; 74:2936-42 Di Pasquale G., Stacey SN Adeno-associated virus Rep78...
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Machinery''''s Handbook P1
... FASTENERS • METRIC THREADED FASTENERS • BRITISH FASTENERS • MACHINE SCREWS AND NUTS • CAP AND SET SCREWS • SELF-THREADING SCREWS • T-SLOTS, BOLTS, AND NUTS • PINS AND STUDS • RETAINING RINGS ... KEYS AND KEYSEATS • FLEXIBLE BELTS AND SHEAVES • TRANSMISSION CHAINS • STANDARDS FOR ELECTRIC MOTORS • ADHESIVES AND SEALANTS • MOTION CONTROL • O-RINGS • ROLLED STEEL SECTIONS, WIRE, AND SHEET-METAL ... extent possible All criticisms and suggestions about revisions, omissions, or inclusion of new material, and requests for assistance with manufacturing problems encountered in the shop are always welcome...
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Machinery''''s Handbook P2
... allows spreadsheets users to easily copy and paste multiple copies of a series of calculations This easy what-if functionality is a cornerstone of spreadsheet usefulness Absolute Addressing: ... ADVANCED SPREADSHEET CONCEPTS 31 problems can be solved using spreadsheets by using a few simple operators and concepts PRACTICE EXERCISES FOR SECTION (See Answers to Practice Exercises For Section ... absolute address is copied and pasted the copied formula maintains the same address as the original The power of this is best illustrated by an example Example 3, Absolute and Relative Addressing...
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Tài liệu Machinery''''s Handbook 27th Edition A REFERENCE BOOK FOR THE MECHANICAL ENGINEER, docx
... FASTENERS • METRIC THREADED FASTENERS • BRITISH FASTENERS • MACHINE SCREWS AND NUTS • CAP AND SET SCREWS • SELF-THREADING SCREWS • T-SLOTS, BOLTS, AND NUTS • PINS AND STUDS • RETAINING RINGS ... KEYS AND KEYSEATS • FLEXIBLE BELTS AND SHEAVES • TRANSMISSION CHAINS • STANDARDS FOR ELECTRIC MOTORS • ADHESIVES AND SEALANTS • MOTION CONTROL • O-RINGS • ROLLED STEEL SECTIONS, WIRE, AND SHEET-METAL ... extent possible All criticisms and suggestions about revisions, omissions, or inclusion of new material, and requests for assistance with manufacturing problems encountered in the shop are always welcome...
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Tài liệu Báo cáo khoa học: Identification of Ewing’s sarcoma protein as a G-quadruplex DNA- and RNA-binding protein ppt
... autoradiography acids, was expressed in Escherichia coli as proteins fused to glutathione S- transferase (GST) and purified using glutathione agarose 32P-labeled ETS-1 was first incubated for 24 ... 4-mediated transcription J Biol Chem 278, 5427–5432 Hume DA, Sasmono T, Himes SR, Sharma SM, Bronisz A, Constantin M, Ostrowski MC & Ross IL (2008) The Ewing sarcoma protein (EWS) binds directly ... support issues arising from supporting information (other than missing files) should be addressed to the authors FEBS Journal 278 (2011) 988–998 ª 2011 The Authors Journal compilation ª 2011 FEBS...
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Tài liệu Báo cáo khoa học: The capsid protein of human immunodeficiency virus: intersubunit interactions during virus assembly doc
... 2009 FEBS M G Mateu Capsid protein interfaces in HIV-1 assembly ´ member of the Instituto de Biocomputacion y Fı´ sica de los Sistemas Complejos, Zaragoza, Spain 15 References Adamson CS & Jones ... hexamers and pentamers using essentially the same structural elements of CA [40]; (e) the different interactions of CA with several ligands and their effects on capsid assembly [3]; and (f) the possibility ... Under these conditions, protein association reactions, such as CA assembly, must be strongly favored [61] The available experimental evidence summarized below is consistent with this prediction...
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Tài liệu Báo cáo khoa học: The capsid protein of human immunodeficiency virus: designing inhibitors of capsid assembly ppt
... drugs such as Bevirimat (Panacos Pharmaceuticals, Watertown, MA, USA) (although it is in Phase II trials) is not discussed because its mechanism of action is not based on inhibition of CA self -assembly ... inhibiting capsid assembly in vivo Acknowledgements Both referees are thanked for their helpful suggestions and discussion This work was supported by grants from Ministerio de Sanidad y Consumo (FIS 01 ... drugs Inhibition of CTD self -assembly by peptides Protein assembly processes can be considered as good targets for antivirals because they depend only on recurring weak interprotein interactions,...
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Tài liệu Báo cáo khoa học: Gas6 and protein S Vitamin K-dependent ligands for the Axl receptor tyrosine kinase subfamily pptx
... Gas6 is up-regulated in growth-arrested cells [4] suggested a role in protection from certain cellular stresses, such as apoptosis Subsequently, many studies demonstrated the ability of Gas6 ... Affinities of Gas6 and protein S for Axl, Sky and Mer RTKs within and across different species (both rat Gas6 and human protein S were shown to stimulate rabbit Sky [17]) The equilibrium dissociation ... in the Gas6–Axl system Several studies, utilizing either site-specific blocking antibodies or partial protein constructs, have established the SHBG region of both Gas6 and protein S as being the...
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Tài liệu Báo cáo khoa học: The role of the ESSS protein in the assembly of a functional and stable mammalian mitochondrial complex I (NADH-ubiquinone oxidoreductase) pptx
... previously been grouped among the ÔancilliaryÕ proteins, also referred to as ÔaccessoryÕ proteins Our present studies establish that the ESSS protein is another essential subunit for assembly ... has no ESSS-HA (as expected), and no signal for the MWFE and PSST subunits We have described previously, that the MWFE subunit is Fig Results from SDS/PAGE, Western blot and BN/PAGE analyses ... cells in which hamster ESSS-HA is expressed and found in a series of-subcomplexes Partial complex I assembly in different respiration deficient mutants of Chinese hamster cells We have investigated...
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Báo cáo khoa học: Mass spectrometric characterization of the covalent modification of the nitrogenase Fe-protein in Azoarcus sp. BH72 ppt
... Pseudomonas aeroginosa ExoS [28] In eukaryotes, mono-ADP-ribosyltransferase reactions are involved in important cellular processes, with substrates such as heterotrimeric G proteins, integrin, histones, ... condition Spots containing nitrogenase Fe- protein are marked by arrows A, Unmodified Fe- protein; B, modified Fe- protein Fig Analysis of both nitrogenase Fe- protein isoforms from conventional Coomassie stained ... Coomassie stain were less sensitive (Fig 2) In the latter case especially, small proteins were scarcely detectable This might have been the result of diffusion during overnight staining because proteins...
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Báo cáo khoa học: In vitro gamma-secretase cleavage of the Alzheimer’s amyloid precursor protein correlates to a subset of presenilin complexes and is inhibited by zinc potx
... overexpress the c-secretase complex components These assays are used to test the effects of detergent concentration, solvents and metals on the c-secretase cleavage of APP substrates These studies show ... of c-secretase activity Lastly, zinc causes structural changes in a c-secretase substrate and acts as an inhibitor of csecretase cleavage of APP Results Design of a novel APP c-secretase substrate ... COS7-SPC993FLAG solubilized membranes To complement our MC99-3FLAG based in vitro c-secretase assays, an in vitro assay using COS7SPC99-3FLAG CHAPSO extracts was developed The substrate in this...
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Báo cáo khoa học: Role of CCP2 of the C4b-binding protein b-chain in protein S binding evaluated by mutagenesis and monoclonal antibodies docx
... is close to CCP1, thus further suggesting a potential role in binding of protein S (d) Residues F114 and I116 are both solvent exposed (e) Residue H108 is also exposed, possibly next to the protein ... b-chain was able to bind MoAb 44 and protein S simultaneously In contrast, immobilized MoAb 44 could not bind the protein S b-chain complex (assay c) These results suggest that protein S and the ... binding of protein S The b-chain constructs were tested in three different assays for their ability to bind protein S In the radioligand blotting assay, proteins were separated by SDS/PAGE, blotted...
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Báo cáo Y học: A b-lysine adenylating enzyme and a b-lysine binding protein involved in poly b-lysine chain assembly in nourseothricin synthesis in Streptomyces noursei pot
... biosynthesis of streptothricins in S noursei [18] The speci®city-conferring residues of the substrate binding pocket of NspA (and SstA) display a sequence with similarity to that of domains known ... ORFs encodes a protein with similarity to the adenylation domains of peptide synthetases This points to nonribosomal mechanisms of streptothricin biosynthesis However, from the data it was not ... preparation using pUC118 or pBlueScriptKS Sequencing was performed on a LiCor automated system Plasmids The plasmid for expression of npsA was pDW5 (K Weber, J Demydchuk, U Peschke, unpublished results)...
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Báo cáo Y học: S-Decyl-glutathione nonspecifically stimulates the ATPase activity of the nucleotide-binding domains of the human multidrug resistance-associated protein, MRP1 (ABCC1) ppt
... thermoacidophilic Sulfolobus solfataricus, by S Albers (Groningen University, the Netherlands) ATPase activity The ATPase activity of NBDs was measured by a colorimetric assay [34] Protein was incubated ... essential to increase solubility of NBD2-fusion proteins Escherichia coli strains DH5a and the protease-poor AD 202 were both used for expression and did not result in large differences In short, an overnight ... in search of a putative interaction of these NBDs with allocrites, we have overproduced these domains as glutathione -S- transferase or maltose binding domain fusion proteins in E coli Both NBDs...
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Báo cáo khoa học: Huntington’s disease: roles of huntingtin-interacting protein 1 (HIP-1) and its molecular partner HIPPI in the regulation of apoptosis and transcription pptx
... increase apoptosis Different symbols representing different proteins are shown in the box Numbers representing different processes are also shown Possible role of HIP-1 and HIPPI in the pathogenesis ... transcription of caspase-1, and this warrants further studies A summary of the findings that HIPPI increases apoptosis and alters gene expression is shown in Fig Hi Pro-caspase-1 45 kDa Caspase-1 20 kDa ... increase their expressions In turn, increased pro-caspase-8 is recruited to HIPPI–HIP-1 heterodimer and increases apoptosis The role of caspase-1 in apoptosis is not known, but in some conditions...
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