false negative result of pcr testing for neisseria meningitidis

... for years postoperatively Page of Obitsu et al Journal of Cardiothoracic Surgery 2010, 5:28 http://www.cardiothoracicsurgery.org/content/5/1/28 Page of results after a mean follow-up period of ... was 3.6%, of which 75% were the result of aneurysm rupture For a second-stage TEVAR after arch repair, a left thoracotomy is not necessary, so early second-stage surgery can be performed; however, ... was obtained from the patient for publication of this case report and accompanying images A copy of the written consent is available for review by the Editor-in-Chief of this journal Acknowledgements...

Ngày tải lên: 10/08/2014, 09:22

... in software development, the quality of the results of manual software testing can vary widely depending on many factors The Top Five Pitfalls of Manual Software Testing Manual software testing ... The Pitfalls of Outsourcing/Offshoring Software Testing 67 Introduction 68 The Top Pitfalls of Outsourcing/Offshoring Software Testing ... 17 An Example of Quality Cost 21 Types of Software Testing 22 The Products of Software Testing 24 What Makes Good Testing? ...

Ngày tải lên: 12/08/2014, 19:20

... manual software testing, even though this is not the most reliable form of testing, sometimes it is the only form of testing available This was true more so for projects that involved GUI testing ... The Pitfalls of Manual Software Testing In the following two chapters, we will discuss: • The Pitfalls of Software Test Automation • The Pitfalls of Outsourcing/Offshoring Software Testing In Chapter ... non-profit organizations, the Association for Software Testing, an organization dedicated to improving the practice of software testing by advancing the science of testing and its application which he...

Ngày tải lên: 12/08/2014, 19:20

... Page of 18 formed by the gp91phox and p22phox proteins The most common form of CGD is X-linked accounting for approximately 70% of cases, due to mutations in the CYBB gene The remaining 30% of ... diagnosis of Crohn’s disease Her family history was remarkable for XL-CGD and ocular complications of CGD Flow cytometric testing for neutrophil oxidative burst revealed populations for DHR fluorescence ... culture was also negative for any pathogenic organisms but positive for leukocytes Colonoscopy showed abnormal wall thickening of all segments of the colon and rectum A diagnosis of severe colitis...

Ngày tải lên: 13/08/2014, 13:22

... Makepeace, K & Doyonnas, R (1996) The N-domain of the human CD66a adhesion molecule is a target for Opa proteins of Neisseria meningitidis and Neisseria gonorrhoeae Mol Microbiol 22, 929–939 ... stopcodon of opaB128 and opaJ129 The PstI–BglII fragments of opaB128 and opaJ129 were used to replace a PstI–BglII fragment of the phoE gene in pMR05, resulting in an inframe fusion of opa to ... an inframe fusion of opa to the signal peptide of phoE and expression from the phoE promotor The resulting plasmids were used for transformation of strain CE1265, which expresses the regulon constitutively...

Ngày tải lên: 17/03/2014, 10:20

... Figure Graphic of the transformation ratio obtained with In A: ratio of transformation of C2135 strain with donor DNA from M2 mutant (ΔNMB0065:: ΩaaDA), In B: ratio of transformation of C2135 strain ... by PCR, searching the presence of target gene transfer in the transforming units (ΩaaDA cassette for the M2 DNA and synG for M6 donor DNA) The graphic of Figure shows significant increase of ... extracted for gene transfer in C2135 receptor strain A preliminary analysis of the action of the mesoporous silica was performed to determine the influence of this nanostructure under Neisseria meningitidis...

Ngày tải lên: 11/08/2014, 00:23

... anatomic integrity of the eye with good results Positive results obtained in Consensus assay were confirmed by targeted PCR (data not shown) [11] Results from the QCMD VZV proficiency panel and ... available The ability of Consensus PCR to yield results from ocular specimens has been of particular interest and of considerable clinical benefit [13,15], especially in cases of hypertensive iridocyclitis ... Finally, negative results after antiviral therapy suggest that the Consensus PCR assay may also be useful for monitoring the efficacy of treatment [20] According to our experience, Consensus PCR assay...

Ngày tải lên: 18/06/2014, 18:20

... anatomic integrity of the eye with good results Positive results obtained in Consensus assay were confirmed by targeted PCR (data not shown) [11] Results from the QCMD VZV proficiency panel and ... available The ability of Consensus PCR to yield results from ocular specimens has been of particular interest and of considerable clinical benefit [13,15], especially in cases of hypertensive iridocyclitis ... Finally, negative results after antiviral therapy suggest that the Consensus PCR assay may also be useful for monitoring the efficacy of treatment [20] According to our experience, Consensus PCR assay...

Ngày tải lên: 20/06/2014, 01:20

... water as a negative control Cycling conditions included a pre-incubation step of at 94 °C followed by 35 cycles of 94 °C for 15 sec, 57 °C for 20 sec, and 68 °C for 15 sec - 38 - Journal of Water ... complement sequence of PAO651 probe Sequence of PAO846r is the same sequence of PAO846 probe For the quantitative PCR, LightCycler (Roche) was employed The final reaction mixture of 20µL contained: ... 30 sec of annealing at 50 °C, and of extension at 72 °C This cycle was repeated 30 times and followed by incubation at 72 °C for 10 for the final extension The initial concentration of PCR products...

Ngày tải lên: 05/09/2013, 09:38

... mechanism of amplification of whole cDNAs and the TSAT -PCR technique (A) Amplification of full-length cDNAs (B) Procedure for the nested PCR reactions This figure is reproduced from [39] Fig Schematic of ... obtain specific PCR products for each of the novel tags using lL of ⁄ 50 and ⁄ 300 dilutions of amplified rSAGE library as template Hot-start and touchdown PCR are highly recommended PCR conditions ... performed in a 96-well plate Two-step analysis of unknown SAGE tags (TSAT -PCR) Given the disadvantages of the above techniques, Xu et al proposed two-step analysis of unknown SAGE tags (TSAT -PCR) ...

Ngày tải lên: 07/03/2014, 00:20

... analysis of CTCs derived from tumors with down-regulation of surface epithelial markers such as EpCAM For this reason, depletion of the leukocyte fraction (CD45 depletion) for enrichment of CTCs ... study, the negative enrichment strategy using depletion of CD45+ leukocytes was compared to positive enrichment of EpCAM+ cells The negative enrichment protocol was applied for detection of CTCs ... Combination of Negative and Positive Enrichment To address whether the combination of both methods may improve results in terms of recovery and purity, a combined protocol consisting of CD45 depletion...

Ngày tải lên: 18/06/2014, 19:20

... analysis of CTCs derived from tumors with down-regulation of surface epithelial markers such as EpCAM For this reason, depletion of the leukocyte fraction (CD45 depletion) for enrichment of CTCs ... study, the negative enrichment strategy using depletion of CD45+ leukocytes was compared to positive enrichment of EpCAM+ cells The negative enrichment protocol was applied for detection of CTCs ... Combination of Negative and Positive Enrichment To address whether the combination of both methods may improve results in terms of recovery and purity, a combined protocol consisting of CD45 depletion...

Ngày tải lên: 20/06/2014, 03:20

... 25’ for group discussion 15’ for summary of the discussion and results of the subject 15’ for giving the lecture by teacher 20’ for group discussion 10’ for summary of the discussion and results ... results of the subject 25’ for discussion giving a group 25’ for representative of each participant group 15’ for summary of lecture and discussions 15.20-15.45 Travel to goat demonstration of project ... extending pasture for goats - Giving a lecture 15’ for lecture 09.45-09.55 09.55-10.50 For participant understood: Briefly about nutrition requirements of goats - Feeds available for goat in the...

Ngày tải lên: 21/06/2014, 06:20

... patients for genetic counseling before testing for these disorders It is very important to discuss the potential advantages and disadvantages of testing with patients before sending blood samples for ... expression, thereby resulting in false negative results Mutations of the cytoplasmic tails of cell surface receptors may impair signaling, while allowing cell surface expression of defective proteins ... the case of carrier testing of minors, testing should only proceed with proper informed consent This guideline also applies to potential female carriers of an X-linked disorder and for predictive...

Ngày tải lên: 08/08/2014, 21:20

... association of this region in our RA family sample Further investigation of this region, including detection and testing of all variants, is required to confirm RA association Page of (page number not for ... haplotypes of the KIAA1109/Tenr/IL2/IL21 gene region These seven haplotypes of the combined five SNPs of the block have a pooled frequency of 96% We observed a nonsignificant undertransmission of the ... the selection of individuals fulfilling the American College of Rheumatology 1987 criteria for RA [14], according to the rheumatologist in charge of the patient Each family consisted of one RA patient...

Ngày tải lên: 09/08/2014, 14:20

... I) for 30 s and 72 ◦ C for 30 s, and to a final extension step of 2.3 Sequencing The sequencing reactions were performed on the PCR products directly using a Dye Terminator kit (Perkin Elmer) For ... U · µL−1 Taq polymerase (Promega) PCR systems and BAC for the Texas marker genes 193 Samples were preheated for at 94 ◦ C, subjected to 35 cycles of 94 ◦ C for 20 s, optimal annealing temperatures ... numbering of each bovine chromosome However, part of these genes were cytogenetically mapped with heterologous probes and specific PCR systems were not available for each marker gene thus preventing PCR- based...

Ngày tải lên: 09/08/2014, 18:21

... training, and by offering testing in the context of comprehensive clinical care, provider-initiated testing offers a unique opportunity to increase access to and acceptance of HIV testing We evaluated ... above, to remain open to offering HIV testing broadly The results of our study demonstrate that it is possible to have a very low rate of missed opportunities for HIV testing in a high-volume, ... opportunities in the context of a minimal package of services during HIV scale up During the time period of review, there were no fixed, specific criteria in place for when to offer HIV testing, however,...

Ngày tải lên: 10/08/2014, 05:20

... gives series of up to four measures (defined by several outcomes, see below) of adherence to the split test procedures for the baseline period and for each of the follow-up periods for each practice ... equipment Execution of external quality assessment analysis is important in order to improve the quality of the point of care testing and is a prerequisite for receiving fees for this service Apply ... Research Unit for General Practice and Section of General Practice, Department of Public Health, University of Copenhagen, Copenhagen, Denmark 2Section of General Practice and Research Unit for General...

Ngày tải lên: 10/08/2014, 11:20

... necessarily optimal Cutoff values are chosen on the basis of cost-benefit analyses, balancing the harms and benefits of false positive and false negative classifications of risk The cut-off defining a ... observations have implications for health care applications of genetic testing, but also for the direct-toconsumer offer of personal genome tests via the internet For health care applications that ... accuracy of the test (for example, sensitivity and PPV) have been described by simple arithmetic formulas [15] These formulas show that the frequency of the risk variant relative to the frequency of...

Ngày tải lên: 11/08/2014, 12:21

... informed consent was obtained from the patient for publication of this case report and any accompanying images A copy of the written consent is available for review by the Editor-in-Chief of ... exclusive of appropriate management for the other We therefore describe various treatment options for managing both anorexia and SMA syndrome, and illustrate how we utilized a variety of resources ... weight, thereby resulting in a body weight of less than 85% of the ideal body weight; (2) patients have an intense fear of gaining weight or becoming fat; (3) the patient has a sense of body image...

Ngày tải lên: 11/08/2014, 17:21