expression of reactive oxygen species in junctional and pocket epithelium

... Determination of intracellular reactive oxygen species as function of cell density Methods Enzymol 352, 91–100 44 Zuo, L & Clanton, T.L (2002) Detection of reactive oxygen and nitrogen species in ... content of GSH (only one-half of that present in A549) and poor GPx activity (Figs and 2); however, RD cells differ significantly in GR activity and in particular in activity, gene expression and ... lower expression of CuZnSOD than the other cells In musclederived cell lines mtDNA ablation reduced the expression and protein amount of mitochondrial MnSOD but not of cytosolic CuZnSOD (Figs and...

Ngày tải lên: 16/03/2014, 18:20

... levels of intracellular reactive oxygen species (ROS) using the 2'-7'-dichlorofluorescein diacetate (CM-H2DCFDA) probe The kinetics of ROS production after short term cultures (30 mn, h and h) ... cells Figure of Analysis thioredoxin expression Analysis of thioredoxin expression A) Western blot analysis of thioredoxin (TRX) expression in PBMCs isolated from BLV-infected (n = 5) and control ... peroxidase and thioredoxin (TRX) [18] Altered expression of these proteins and disruption of ROS homeostasis has been involved in the development of virus-induced malignancies [26] Intracellular overexpression...

Ngày tải lên: 12/08/2014, 23:22

... xenobiotic and ROS signalling are discussed Results Patterns of accumulation of singlet oxygen, superoxide radical and hydrogen peroxide The transfer of plantlets after weeks of growth to control and ... 57:711-738 Asada K: Production and scavenging of reactive oxygen species in chloroplasts and their functions Plant Physiol 2006, 141(2):391-396 Bowler C, Slooten L, Vandenbranden S, De Rycke R, Botterman ... detection of hydrogen peroxide and singlet oxygen: its use for the assay of reactive oxygen species produced in vivo Plant J 2007, 50(3):475-487 Chao L, Zhou QX, Chen S, Cui S, Wang ME: Single and...

Ngày tải lên: 12/08/2014, 03:20

... Medicine and Toxicology 2009, 4:17 Figure by neutrophils in contact with PM2.5 In vitro generation of reactive oxygen and nitrogen species In vitro generation of reactive oxygen and nitrogen species ... susceptibility of lipids to oxidation was used to calculate the sample size According to the mean comparison formula [15] with a standard deviation of 157.53 and a difference of 616, Za of 95% and a Zb of ... modification of molecules and/ or disruption of cellular structures and tissue injury [9] Due to high MPO activity, the generation of hypochlorous acid (HOCl) and reactive nitrogen species (RNS)...

Ngày tải lên: 20/06/2014, 00:20

... was 88% Expression of UCP3 protein in SS and IMF mitochondria of various muscles Figure 3A illustrates the distribution of UCP3 in SS and IMF mitochondria obtained from different types of mouse ... amounts of UCP3 protein and mRNA in the three muscles are comparable Effect of fasting on UCP3 protein expression We and others have shown that fasting induces upregulation of UCP3 mRNA expression ... Transfected cells Species (muscle mitochondria) Vidal-Puig et al [6] Peptide sequence between TM3 and TM4 of mouse and rat UCP3 (Lilly) C-Terminus of human UCP3, AB3046 (Chemicon) C-Terminus of human...

Ngày tải lên: 24/03/2014, 04:21

... medication TLR4 expression is down regulated in the nasal epithelium of smokers in-vivo We examined expression of TLR4 along with TLR2 and HBD2 in the nasal epithelium of healthy smokers and age matched ... TLR4 mRNA and protein expression and quantifying IL-8 expression in airway epithelial cells stimulated with cigarette smoke extracts The effects of other potential modulators of TLR expression ... chronic inflammation and acute exacerbations This study examined the expression of TLR4 and HBD2 in respiratory epithelium in non-smokers and smokers with COPD The effect of cigarette smoke was...

Ngày tải lên: 12/08/2014, 15:21

... the nomenclature of free radicals, the term 'reactive oxygen species' (ROS) has prevailed, although ROS can be differentiated into reactive nitrogen species and other oxidant species The relationship ... expression of VEGF, VEGF-121 and VEGF-165 splice variants and VEGF receptors (VEGFR-1 and VEGFR-2) after stimulation by ROS donors Knowledge of this expression could lead to a better understanding ... Stimulation of immortalized chondrocytes (a) 1) and (c) and articular cartilage explants (b) and (d) with PMA (μg per ml of medium) or SIN-1 (μM) VEGFR-1 is undetectable in (b) mRNA expression of VEGF,...

Ngày tải lên: 09/08/2014, 08:23

... and reactive nitrogen species (RNS) production The increased production of reactive species was apparent after h of stimulation Since UCP2 is described as a regulator of ROS production, the expression ... inability of MB and LPS to induce any impairment in mitochondrial function after h of treatment RCR and states 2, 3, and FCCP rates of MB- and LPStreated cells were the same as those of unstimulated ... 0.05) and a return to baseline level after h of treatment (Fig 4B) Relative expression of UCP2/GAPDH Macrophage activation by MDP leads to overexpression of UCP2 A UCP2 Relative expression of UCP2/GAPDH...

Ngày tải lên: 05/03/2014, 23:20

... upregulated upon 48 h of hypoxia, and that this increase is dependent on the tissue and the severity of hypoxia The mechanism of induction of Cygb is regulated by HIF-1 The aim of this study was ... dependency and the tissue specificity of Cygb and Ngb mRNA upregulation upon hypoxia and after reoxygenation In addition, we tried to shed light on the ROS-scavenging function of Ngb and on the ... Cleaved caspase-3 protein expression in heart and liver under hypoxic (H) conditions (48 h) and after reoxygenation (48R8, 48R24: 48 h of hypoxia, followed by h or 24 h of reoxygenation) Positive...

Ngày tải lên: 23/03/2014, 09:21

... and acts as a promoter for both the heavy and light strands of the mtDNA, and contains essential transcription and replication elements The D-loop region is a hot spot for mtDNA alterations and ... in the detection of absorbance The experiment was repeated times and data were expressed as Χ± SD Detection of CagA and VacA protein using SDS-PAGE and Western Blot Detection of cell viability ... microliters of extracts were mixed thoroughly with 20 μl of loading buffer, which were then boiled for Ten microliters of the mixture were subjected to SDS–PAGE, and bands were captured Treatments of...

Ngày tải lên: 25/10/2012, 11:18

... chain of this residue forming hydrogen bonds with oxygens of the 3-phosphate; NH1 and NH2 of Arg105 form hydrogen bonds with the 4-OH and 5-OH of the inositol moiety; and the aromatic ring of Tyr59 ... in a hydrophobic pocket of SH3(N), and Tyr324 probably points into a hydrophobic pocket formed by the linker region and SH3(C) (Fig 11) Intriguingly, the amino acids of the AIR and FEBS Journal ... a-helices of TPR1 and TPR2 (position of TPR2) and Asn204 in the b-hairpin (position 34 of TPR3), are highly conserved among p67phox and its related proteins (Fig 14) In addition, substitution of Glu...

Ngày tải lên: 16/03/2014, 06:20

... evaluating spontaneous breakage and distribution of common fragile sites in two Ceboidea species Fragile sites expression in Ceboidea 89 MATERIALS AND METHODS C-fra expression was analyzed in heparinized ... chromosome events and dicentric or triradial configurations as two chromosome events G-band analysis of control cultures allowed the identification of and 39 spontaneous CAs in SBO and ACA specimens, ... demonstrated that any band with or more lesions was non-randomly damaged (p < 0.01), identifying bands: 1q23, 2q13 and 11q19 which were hot-spots for spontaneous breakage A total of 245 and 328 CAs was...

Ngày tải lên: 09/08/2014, 18:21

... Generation of reactive oxygen species and induction of apoptosis Journal of carcinogenesis 2009, 8:9 26 Lin Y, Shi R, Wang X, Shen HM: Luteolin, a flavonoid with potential for cancer prevention and ... SA, Lin Y: A critical role of luteolininduced reactive oxygen species in blockage of tumor necrosis factoractivated nuclear factor-kappaB pathway and sensitization of apoptosis in lung cancer ... each figure For morphological study of cell death, cells were stained with 50 μg/mL of acridine orange and 50 μg/mL of ethidium bromide and then observed and photographed under a fluorescent...

Ngày tải lên: 10/08/2014, 10:20

... Maier M, Landthaler M, Szeimies RM, Baumler W: The role of singlet oxygen and oxygen concentration in photodynamic inactivation of bacteria Proceedings of the National Academy of Sciences of the ... individual detection of hydrogen peroxide and singlet oxygen: its use for the assay of reactive oxygen species produced in vivo Plant Journal 2007, 50:475-487 88 de Torres M, Sanchez P, Fernandez-Delmond ... mutant and repressed in wild-type plants (Table 5) It is well known that the transcription and the translation of PSII and PSI genes is extremely complex and often uncoupled Analysis of the barley...

Ngày tải lên: 11/08/2014, 11:22

... Program of the NIH, National Institute for Dental and Craniofacial Research References Detection of ROS in culture supernatant The amount of reactive oxygen species present in the supernatants of ... Control of Bcl-2 expression by reactive oxygen species Proc Natl Acad Sci USA 2003, 100:15035-15040 Hildeman DA, Mitchell T, Teague TK, Hanson P, Day BJ, Kappler J, Marrack PC: Reactive oxygen species ... late stage of T cell activation, following maximal secretion of most Th1 and Th2 cytokines, may resolve the current paradox of TCR and CD28 induction of Foxp3 (a regulatory T cell gene) and consequent...

Ngày tải lên: 13/08/2014, 05:22

... SCHOOL Research Integrity and Copyright Disclaimer Title of Thesis/Dissertation: Bone Metabolism: The Role of STAT3 and Reactive Oxygen Species For the degree of Master of Science Choose your degree ... ROLE OF STAT3 AND REACTIVE OXYGEN SPECIES A Thesis Submitted to the Faculty of Purdue University by America Bethanne Newnum In Partial Fulfillment of the Requirements for the Degree of Master of ... University, August 2012 Bone Metabolism: The Role of STAT3 and Reactive Oxygen Species Major Professor: Jiliang Li Signal Transducers and Activators of Transcription (STAT3), a transcription factor...

Ngày tải lên: 24/08/2014, 12:59

... machinery I.2 Reactive oxygen species and apoptosis I.2.a Pro and Anti-Apoptotic functions of ROS I.2.b Rac1 and superoxide anion production 11 I.2.c Superoxide anion and inhibition of apoptosis ... modifications of many of the proteins involved in apoptotic machinery (Dechao L et al 2004; John JH, 2004; Irani K and Pascal JGC, 1998) I.2 Reactive Oxygen Species and Apoptosis Cells generate reactive oxygen ... One of the hallmarks of tumor development and maintenance is defiance of tumor cells to execute death signals (Thompson CB, 1995) Thus, a combination of increased proliferation and lack of cell...

Ngày tải lên: 16/09/2015, 08:31

... Modulation of cellular redox status 26 (iv) Autophagy and metabolism (v) RSV and Reactive oxygen species (ROS)/NADPH Oxidase (NOX) 1.5: Aim of study 29 1.6: Mode of action of ... dioxide, and small amounts of other gases Air also contains a variable amount of water vapor (an average of 1%) Reactive oxygen species (ROS) are oxygen- derived small molecules (Bedard and Krause, ... the p53 gene expression levels and results in growth arrest and activation of gluconeogenic signaling pathways (Pervaiz and Holme, 2009) (vi) Resveratrol (RSV) and Reactive oxygen species (ROS)/NADPH...

Ngày tải lên: 26/09/2015, 10:44

... 43 Expression of α7 and β2 integrins on neutrophils from wild type and gp91phox knock-out mice after being challenged with thioglycollate for hours 44 MFI of α7 and β2 integrins of blood and ... application of caecal contents and again examined the level of expression of these markers on blood neutrophils Both C57BL6 and gp91phox knock-out blood neutrophils elevated the expression level of α7 ... peritoneal inflammation Many different models of peritoneal inflammation exist and each of them has its own kinetics of cellular influx and of development of the inflammatory response To try to place...

Ngày tải lên: 21/05/2016, 22:38

... prevention and treatment of many diseases [2] Reactive oxygen species (ROS), a group of highly reactive molecules, including singlet oxygen, hydroxyl radicals, superoxide anion, nitric oxide and hydrogen ... 500 lL of NaCl ⁄ Pi and 10 mL of 70% ethanol at °C overnight; then, after washing twice with NaCl ⁄ Pi, the cells were incubated with mL of PI staining solution (50 mgÆL)1 of PI and gÆL)1 of RNase ... endogenous production of reactive oxygen species Cancer Res 65, 948–956 12 Pan J, She M, Xu ZX, Sun L & Yeung SC (2005) Farnesyltransferase inhibitors induce DNA damage via reactive oxygen species in human...

Ngày tải lên: 18/02/2014, 13:20