expression of iswi and acf via baculovirus vectors
Báo cáo sinh học: " Influence of insulators on transgene expression from integrating and non-integrating lentiviral vectors" docx
... luciferase expression cassette (see Additional File for vector design) Three types of cells (HEK293T, Hela and NIH-3T3) were transduced in Page of triplicate with a series of doses of the vector, and ... lentiviral vectors Three types of cells (HEK293T, Hela and NIH-3T3) were transduced in triplicate with a series of doses of the vector (ranging from 0.1 to 15 ng of p24, measured by ELISA), and luciferase ... Figures 2a and 2b for which an unpaired t test was used Results and discussion We studied the effects of the incorporation of the Ig MAR on transgene expression from three types of LV containing...
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Tài liệu Báo cáo khoa học: Bioimaging of the unbalanced expression of microRNA9 and microRNA9* during the neuronal differentiation of P19 cells doc
... for for for for for for for expression of pri-miR9-1 expression of pri-miR9-1 expression of pri-miR9-2 expression of pri-miR9-2 expression of pri-miR9-3 expression of pri-miR9-3 neuronal differentiation ... et al Bioimaging of miR9 and miR9* during neurogenesis higher endogenous level of mature miR9 than of mature miR9* In vivo visualization of unbalanced expressions of mature miR9 and miR9* during ... Gene expressions of mature miR9 and miR9* during neuronal differentiation (A) Using real-time PCR The gene expressions of endogenous mature miR9 and miR9* during the neuronal differentiation of...
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Tài liệu Báo cáo khoa học: Adaptive changes in the expression of nuclear and mitochondrial encoded subunits of cytochrome c oxidase and the catalytic activity during hypoxia pptx
... switch and alters the expression of the two nuclear genome coded isoforms of CytOX V [10,32] The regulation of genes CytOX5a and CytOX5b, coding for the two isofoms Va and Vb, parallels that of genes ... different stages of adaptive response to hypoxia Based on the results of our study and those of Schumacker, it is reasonable to conclude that the effects of hypoxia on CytOX gene expression and its activity ... co-expressed under hypoxic (O2 < 0.5 lM) and heme deficient conditions [11] The coexpression of specific subunit V and cytochrome c isoforms indicates that these isoform pairs act synergistically to...
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Báo cáo khoa học: The expression of retinoblastoma and Sp1 is increased by low concentrations of cyclin-dependent kinase inhibitors ppt
... Rb and Sp1 protein caused by CDK inhibitors in CHO cells The increase in total levels of Rb and Sp1 proteins caused by UCN-01 may be a result of the enhanced transcription and stability of Rb and ... maximal expression of Rb and Sp1, and control and UCN01-treated cells were then incubated with 50 lgÆmL)1 cycloheximide for different periods of time Whole protein extracts were prepared and used ... Biochem 270) 4813 Fig Effects of UCN-01 on the levels of Rb and Sp1 protein (A), (B) Dose–response of the effect of UCN-01 on the levels of retinoblastoma gene product (Rb) and Sp1 proteins, respsectively...
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Báo cáo khoa học: Differential expression of liver and kidney proteins in a mouse model for primary hyperoxaluria type I pdf
... protein expression within the liver and kidney of Agxt) ⁄ ) deficient mice 4768 ´ J R Hernandez-Fernaud and E Salido compared with wild-type controls by 2-DE separation and MS The analysis of specific ... bisphosphatase and catalase, whereas liver enolase and carbonic anhydrase were downregulated Comparable amounts of b-actin were present in AgxtKO and wild-type cytosolic fractions, and the absence of AGT1 ... this model, and provide evidence useful in substrate depletion strategies, we report in this article a proteomic analysis of the changes in expression levels of various enzymes of liver and kidney...
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Báo cáo khoa học: Dual expression of mouse and rat VRL-1 in the dorsal root ganglion derived cell line F-11 and biochemical analysis of VRL-1 after heterologous expression pptx
... immunoreactivity band were observed, only the 80 kDa band showed up with strong intensity (B) and (C) Anti-GFP and anti-VRL-1 Western blot of F-11 cells expression VRL-1-GFP The same band pattern of the ... vector (Invitrogen) and sequenced The accuracy of sequences from the open reading frame was checked by DNA sequencing of both strands The resulting sequences of specific bands were identical to ... new codon for P761 and a stop codon The accuracy of the resulting new plasmid pcDNA3.1/rVRL-1 was confirmed by DNA sequencing of both strands Cell culture and transfection of F-11 cells F-11 cells...
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Báo cáo sinh học: " Persistent expression of chemokine and chemokine receptor RNAs at primary and latent sites of herpes simplex virus 1 infection" pptx
... HSV induces expression of IL-6 [18], and infection of macrophages by HSV induces RANTES expression directly [19] Infection of other cell types may induce expression of other cytokines and chemokines ... and 2; RANTES; MCP-2, 3, and 4; HCC-2 RANTES; MIP-1α and 1β Migration of DC to sites of inflammation Recruitment of T cells, macrophages and NK Migration of effector T cells (Th1) Migration of ... 16.2 17.0 17.5 16.6 15.8 Figure and ICP0 RNA expression in mock and HSV-infected cornea and TG HSV tk HSV tk and ICP0 RNA expression in mock and HSV-infected cornea and TG RNA isolated from tissues...
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báo cáo hóa học: " 5-aminoimidazole-4-carboxamide-1-beta-4-ribofuranoside (AICAR) attenuates the expression of LPS- and Aβ peptide-induced inflammatory mediators in astroglia" pptx
... shows the expression of iNOS, MnSOD and COX-2 in glial cells Aβ peptide upregulated the LPSmediated expression of MnSOD Aβ peptides (1–40) and (1–42) induced the expression of iNOS, COX-2, and MnSOD; ... terms of cytokine release and iNOS, COX-2 and MnSOD expression (Fig and 4) as well as a dose-dependent inhibition with AICAR Figure 3A shows the dose-dependent expression of TNF-α, IL-1β and IL-6 ... cytokines TNF-α and IL-1β by activated glial cells (Figures and 3), is consistent with expression of these cytokines in brains of experimental animal models of Alzheimer's and in the brain of Alzheimer's...
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Báo cáo khoa học: "Enhanced expression of constitutive and inducible forms of nitric oxide synthase in autoimmune encephalomyelitis" pdf
... Western blot analysis of three isoforms of NOS in EAE The expression of nNOS (Fig 2A), eNOS (Fig 2B) and Fig Western blot analysis of constitutive neuronal NOS (nNOS) (A) and endothelial NOS (eNOS) ... B and C, ×132 A: TUNEL and hematoxylin, B: TUNEL and either rabbit anti-GFAP, C: TUNEL and rabbit and ED1 Immunohistochemical localization of nNOS, eNOS, and iNOS in EAE In the spinal cords of ... cells in the EAE lesions of the spinal cord (Fig 4D) The expression of eNOS was observed in the endothelial cells of blood vessels and some astrocytes (Fig 4E) The expression of iNOS (Fig 4F) was...
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báo cáo khoa học: " All-trans retinoic acid inhibits KIT activity and induces apoptosis in gastrointestinal stromal tumor GIST-T1 cell line by affecting on the expression of survivin and Bax protein" pdf
... on expression of survivin, XIAP and Bax protein It is well known that apoptotic process is regulated by many factors We investigated the expression of inhibitors of apoptosis, survivin, XIAP, and ... on the expression of survivin and Bax Panel A shows the down-regulated expression of survivin after 2-, 4- or 6-day treatment with 180 μM ATRA Panel B shows the up-regulated expression of Bax ... of survivin and up-regulation of Bax (Figure 3A and 3B) Even though XIAP and survivin belong to the same family of apoptotic inhibitors, it is likely that ATRA effected quite differently on expression...
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báo cáo khoa học: "Low-level expression of HER2 and CK19 in normal peripheral blood mononuclear cells: relevance for detection of circulating tumor cells" doc
... differences in expression between normal and tumor cells may be better appreciated[29] In addition, the quantitation of expression may allow assessment of expression levels of the target and provide ... understand the source of the HER2 and CK19 signals in peripheral blood, we isolated subpopulations from the PBMC fraction and characterized them for HER2 and CK19 Understanding the biology of the ... development of improved methods of negative and positive selection to remove the source of background signals from peripheral blood samples Non invasive and highly specific and sensitive methods of detecting...
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Báo cáo khoa hoc:" Expression of estrogen and progesterone receptors in vestibular schwannomas and their clinical significance" docx
... all brain tumors (Figure 1) Expression of estrogen and progesterone receptors and their potential role in the progression of vestibular schwannoma is still an area of controversy Many diverging ... and residual cases where complete excision is not feasible The objective of the present study was to retrospectively determine the expression of estrogen and progesterone receptors by means of ... Studies on the expression of estrogen and progesterone receptors in vestibular schwannomas have seldom been reported Moreover, conflicting results about the expression of estrogen and progesterone...
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Báo cáo y học: "Cigarette smoke regulates the expression of TLR4 and IL-8 production by human macrophages" pdf
... phosphorylation of and degradation of CSM regulates phosphorylation of IRAK IRAK in HEK cells CSM regulates phosphorylation of IRAK and degradation of IκB-α by MDMs and phosphorylation of IRAK in ... CSM regulates expression of TLR4 via ROS CSM regulates expression of TLR4 via ROS (A) MDMs (5 × 106 cells) were stimulated by CSM (0.03, 0.06 and 0.12 OD) for h with and without pretreatment ... the expression of TLR4 The authors declare that they have no competing interests Authors' contributions HS and EM equally conceived of the study, and participated in the design of the study and...
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Báo cáo y học: "Impaired expression of mitochondrial and adipogenic genes in adipose tissue from a patient with acquired partial lipodystrophy (Barraquer-Simons syndrome): a case report" pot
... overall clinical and biochemical features of the patient led to the diagnosis of APL The pattern of progressive loss of subcutaneous adipose tissue in the face and the upper part of the body was ... Spain Separate analysis of gene expression markers in men and women did not show any significant difference and therefore reference values of gene expression in healthy men and women were shown ... ratio of relative abundance of the mRNA of the gene of interest respect to 18S rRNA (Hs99999901_s1) Examination of gene expression for master regulatory factors associated with the promotion of...
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Báo cáo y học: "B cells from rheumatoid arthritis patients show important alterations in the expression of CD86 and FcgRIIb, which are modulated by anti-tumor necrosis factor therapy" potx
... College of Rheumatology criteria for RA [19] All of the patients were women, with a mean ± standard deviation (SD) age of 52.8 ± 10.5 years and disease duration of 16.3 ± years at study entry All of ... cells and plasmablasts by means of CD19 and CD27 staining, we detected similar percentages of these subsets in the two groups of individuals (Figure 1) Higher frequency of activated naïve and memory ... http://arthritis-research.com/content/12/2/R68 Page of 11 Figure Increased expression of CD86 on naïve and memory B cells from rheumatoid arthritis (RA) patients (a) Dot plots representative of the expression of CD86 on B cells...
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Báo cáo y học: " Expression of endothelia and lymphocyte adhesion molecules in bronchus-associated lymphoid tissue (BALT) in adult human lung" pot
... Semi-serial frozen sections of BALT were stained with mAbs against CD19 and CD3 (A), CD4 and CD8 (B), CD4 and CD45RO (C), and CD4 and CD45RA (D) L is in the airway lumen of the bronchus (A) B cell ... HEVs of tertiary lymphoid tissues but not of LNs and PPs [52] Thus, BALT is not identical to "conventional" secondary lymphoid tissues such as LNs and PPs A limitation of our study and of several ... mouse BALT; and ICAM-1 and ICAM-2, which are expressed by HEVs in LNs, PPs and mouse BALT [1,9] For comparison to BALT, we also stained sections of PP There was strong expression of PNAd on all...
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Báo cáo y học: "Could the expression of CD86 and FcγRIIB on B cells be functionally related and involved in driving rheumatoid arthritis" pps
... [6] The link between the inhibitory FcγRIIB and the modulation of B cell activity and humoral tolerance has also been translated into humans Expression of FcγRIIB was found to be considerably decreased ... Catalán and colleagues did not show any stringent relationship between B cell activation measured by CD86 expression and levels of FcγRIIB, indicating that the relationship between FcγRIIB expression ... discrepancy between FcγRIIB expression and B cell activation as determined by CD86 expression Page of Finally, if FcγRIIB truly acts as a late checkpoint at the level of class-switched B cells...
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Báo cáo y học: " APOBEC3G-UBA2 fusion as a potential strategy for stable expression of APOBEC3G and inhibition of HIV-1 replication" pot
... anti-HA, and anti-β-actin antibodies respectively A-b The intensity of APOBEC3G protein and value of the relative intensity of APOBEC3G was determined as described in Fig Note, a protein band that ... total number of blue cells with the total number of cells counted Data shown represent average of three independent experiments Error Bars shown are standard errors of the means a results of wild ... contributions LL and DL carried out all of the experiments JYL provided supervision of the study and co-mentored LL's Ph.D dissertation RYZ supervised and directed the designed studies and co-mentored...
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Báo cáo y học: "Expression of AFP and Rev-Erb A/Rev-Erb B and N-CoR in fetal rat liver, liver injury and liver regeneration" doc
... fetal rat liver Expression of AFP gene and Rev-Erb A, Rev-Erb B and NExpression of AFP gene and Rev-Erb A, Rev-Erb B and N-CoR in fetal rat liver The expression of the AFP gene (A) and of Rev-Erb ... CoR in the rat model of acute liver damage Expression of AFP gene and Rev-Erb A, Rev-Erb B and NExpression of AFP gene and Rev-Erb A, Rev-Erb B and N-CoR in the rat model of acute liver damage ... of hepatocarcinogenesis and non- Figure liverof AFP gene and Rev-Erb A, Rev-Erb B and NCoR in Expression regeneration without 2-AAF treatment Expression of AFP gene and Rev-Erb A, Rev-Erb B and...
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