expression of heparan sulfate sulfotransferases and epimerase

... p21/WAF1, and MDM2 were mainly located in the nuclei of the cancer cells and highly expressed in the HCC Figure Expression of p53, p21/WAF1 and MDM2 in HCC by IHC Expression of p53, p21/WAF1 and MDM2 ... proportions of p53, p21/WAF1, and MDM2 expression in HCC tissues were 70.7% (128/181), 33.1% (60/181), and 52.5% (95/181), respectively Positive proportions of p53, p21/WAF1, and MDM2 expression ... that the expression levels of p53, p21/WAF1, and MDM2 were higher in the HCC tissues than those in the adjacent hepatic tissues The expression of p53, p21/WAF1, and MDM2 in the 181 pairs of tissues...

Ngày tải lên: 18/06/2014, 15:20

... and returned on day 14 to the level of control mice at day The expression of cystatin B was increased about twofold on day of the acute stage (Fig 3a) Like the expression of cathepsins, the expression ... volume of 25 µl, consisting of 2.5 µl of 10 × PCR buffer, 2.0–2.5 µl of MgCl2 (25 mM), 0.5 µl of dNTPs (10 mM each; Promega), 0.5 µl of Hot Star Taq (5 U/µl), 0.5 µl of primer mix (20 µM each) and ... days and The downregulation of cathepsin K on day could be another example of the fact that the time course of cytokine expression determines the time course of proteinase and inhibitor expression...

Ngày tải lên: 09/08/2014, 06:22

... between gene expression and bone architecture were explored The results of the data are indicative of the generalised skeletal distribution of primary OA pathology and suggest that gene expression ... between gene expression and histomorphometric indices were made independent of gender for the OA and control groups Bone anabolic and collagen type I mRNA expression between OA and control individuals ... differential gene expression of the α1 and α2 chain of collagen type I (p < 0.0001 and p < 0.002, respectively) between OA and controls, are shown in Table Interestingly, the ratio of COL1A1/COL1A2...

Ngày tải lên: 09/08/2014, 08:23

... new member of the tumor necrosis factor (TNF) family and prevents apoptosis, increases survival, and stimulates cytokine production in dendritic cells [17,18] TRANCE and the ligand of RANK (RANKL) ... local immune response and bone remodeling, such as those of the RANK/RANK-L pathway To address this question, we investigated the expression of RANK-L, OPG, RANK, and TRAF6 Page of 12 (page number ... prednisone, and two were taking disease-modifying anti-rheumatic drugs Due to limited quantities of SF and neutrophils from patients with RA and due to the requirement of large numbers of cells...

Ngày tải lên: 09/08/2014, 10:20

... Induction of TNFα and MMP-13 expression by AGE-BSA in human osteoarthritis chondrocytes OA chondrocytes were treated with increasing doses of AGEBSA (20 to 600 μg/ml) and the mRNA expression of TNFα and ... clear bands on a background of uniform light blue staining Electrophoretic migration of MMP-13 in the supernatant was compared with known molecular weight standards and also with clear bands of MMP-13 ... Densitometric analysis Measurements of the scanned bands were performed using Un-Scan-It software Each band was scanned five times, and the mean band intensity (pixels per band) was obtained Data were...

Ngày tải lên: 09/08/2014, 14:20

... had a lower level of expression of these three genes than PC12 (WT-Akt) cells, and also demonstrated that pharmacological inhibition of Akt causes an increase in the expression of these genes in ... the expression of MafK, SytI, and Syn-1 genes, all of which are positively related to neuronal function Methods Materials A potent and specific inhibitor of Akt (AKTi-1/2; naphthyridinone) and ... indicate that Akt downregulates the expression of MafK, SytI, and Syn-1 MafK and SytI are involved in certain aspects of neuronal function Inhibition of MafK expression has been reported to suppress...

Ngày tải lên: 10/08/2014, 05:21

... grades of caspase-3 and p53 expression (Figures and 2, respectively) (P > 0.05) However, the frequency of grade 3+ expression of Bcl-2 was significantly higher in the GAP group than that of the ... frequency of caspase-3 expression or frequencies of different grades of expression of p53 However, staining for Bcl-2 was more frequent in the GAP specimens than in the controls Certain activators of ... contributions SB concieved and coordinated the study and participated in the collection of sample and data; and writing the manuscript HU participated in the collection of samples and writing the manuscript...

Ngày tải lên: 11/08/2014, 23:22

... Finally, the role of prostaglandins in physiology and immune system is determined by multiple factors such as cellular context, receptor expression profile, receptor-ligand affinity and differential ... 300 nM of primer pairs and a set of primers specific for this hIL-5Rα isoform [16] PCR protocol consisted of cycle at 95°C for 10 minutes followed by 40 cycles at 95°C for 30 seconds and at 64°C ... mRNA expression of eicosanoid receptors by real time PCR Expression of leukotrienes and prostanoid receptors analyzed by quantitative real time PCR showed an up-regulation of CysLT1 and CysLT2 receptors...

Ngày tải lên: 12/08/2014, 16:20

... of 100 μl of whole blood from patients and controls were stained with μl of CD14-PerCP and μl of CD66-FITC The tubes were also stained with the following isotypes: 10 μl of mIgG2a-PE and μl of ... CD11c and CD66b expression on neutrophil surface There were no differences in the expression of CD11b and CD11c among the four groups (p = 0.45 and p = 0.34, respectively) (Figure 1C,D) The expression ... the surface cell expression of TLR2 and TLR4, the prototypes of PRRs for Gram-positive and Gram-negative bacteria, CD66b, a selectin with signaling functions [29], and CD11b and CD11c, integrins...

Ngày tải lên: 13/08/2014, 10:20

... inhibitor of ATP sulfurylase and hence of the production of phosphoadenosine phosphosulfate (PAPS), the active sulfate donor for sulfotransferases It has been shown to abolish sulfation on proteins and ... synthesis ([5], and our results) HSPG and especially glypicans and syndecans have been described as coreceptors for this growth factor via a highly sulfated sequence of their heparan sulfate chains ... method of Chomczynski & Sacchi [29] using Trizol reagent The integrity and quality of puri®ed RNA were controlled by 1% agarose gel electrophoresis and measure of the absorbance at 260 and 280...

Ngày tải lên: 21/02/2014, 03:20

... of contents    Chapter Expression and Functional Analysis of Heparan Sulphate 189 in Prostate Cancer 5.1 5.1.1 Expression analysis of heparan sulphate in prostate cancer 189 Localization of heparan ... addition of GlcA and GlcNAc N-deacetylation and N-sulphation of GlcNAc units N-deacetylation and N-sulphation of GlcNAc units N-deacetylation and N-sulphation of GlcNAc units N-deacetylation and N-sulphation ... List of Publications xxiii Chapter Introduction 1.1 Prostate gland and prostate cancer 1.1.1 The anatomy of the prostate gland 1.1.2 Functions of the prostate gland 1.1.3 The histology and histopathology...

Ngày tải lên: 12/09/2015, 11:08

... influences of adiponectin on insulin resistance, type diabetes, and cardiovascular disease Diabetes 2007; 5: 1198-209 Hu XB, Zhang YJ, Zhang HT, et al Cloning and expression of adiponectin and its ... sonicated and centrifuged to separate the supernatant and inclusion body Part of the production was applied to 12% SDS–PAGE analysis and Western blot analysis (A) SDS-PAGE analysis: Most of the ... the C-terminus of GLP-1-A Compared with native GLP-1, the fusion protein has a modified site and larger molecular size, and may circumvent the rapid inactivation and renal clearance of GLP-1 Studies...

Ngày tải lên: 25/10/2012, 11:10

... of amoA mRNA in Run was observed due to low dosage of polyferric sulfate This means that the impact of low concentration of polyferric sulfate on amoA mRNA expression is limited, and dosage of ... investigation Number of ammonia oxidizers and copy number of amoA mRNA The time courses of the copy number of amoA mRNA and the number of ammonia oxidizer are shown in Fig No significant change and difference ... amoA mRNA The difference of the effect of polyferric sulfate on each species of ammonia oxidizer was demonstrated by T-RFLP analysis of cDNA of amoA mRNA The profiles of T-RFLP in Run are shown...

Ngày tải lên: 05/09/2013, 10:15

... intensity of the ethidium bromidestained band with the marker bands A total of 30–90 ng of DNA is recommended for direct sequencing of PCR products Analysis, sequencing and in vitro expression of PCR ... primers Of course in this case the use of an enzyme with 3′-exonuclease proofreading activity should be avoided to protect the differentiating 3′-end An example of the design of original and nested ... giving a clear result and is relatively rapid and simple requiring mixing of an aliquot of PCR product, 10× restriction buffer and restriction enzyme, incubation to allow digestion and then agarose...

Ngày tải lên: 25/10/2013, 22:20

... [19], and NMR spectroscopic data of p25a homologs from mice, Caenorhabditis elegans and humans [20–22] demonTable Determination of Vmax and Km for DAT, NET and SERT in the presence and absence of ... loss of survival Co -expression of DAT and p25a increased this cell loss, but co -expression with AS attenuated it Bars represents means ± SD of triplicates in one representative experiment of three ... membrane expression of DAT A potential effect of p25a on DAT function was addressed by expressing DAT alone and in the presence of p25a in HEK-293-MSR cells To validate the specificity of the C-20...

Ngày tải lên: 14/02/2014, 21:20

... homologous expression of the nrdF gene of C ammoniagenes strain ATCC 6872 This is the first report of the successful purification of high amounts of the native C ammoniagenes R2F as a manganese- and tyrosyl ... levels of R2F after h of incubation in the presence of 0.6 mm IPTG This amounted to 5% of the total cellular protein as assessed by SDS ⁄ PAGE and immunodetection (see above) The high expression of ... from the nrdF sequence of C ammoniagenes ATCC 6872 [10] and subsequent purification of the IgG fraction In-gel digestion of the R2F band and protein identification by Q-TOF MS-MS was performed...

Ngày tải lên: 15/02/2014, 01:20

... for for for for for for for expression of pri-miR9-1 expression of pri-miR9-1 expression of pri-miR9-2 expression of pri-miR9-2 expression of pri-miR9-3 expression of pri-miR9-3 neuronal differentiation ... et al Bioimaging of miR9 and miR9* during neurogenesis higher endogenous level of mature miR9 than of mature miR9* In vivo visualization of unbalanced expressions of mature miR9 and miR9* during ... Gene expressions of mature miR9 and miR9* during neuronal differentiation (A) Using real-time PCR The gene expressions of endogenous mature miR9 and miR9* during the neuronal differentiation of...

Ngày tải lên: 18/02/2014, 17:20

... loading of the liver epithelial cell line HepG2 influenced the expression of immune regulatory molecules known to function as ligands of selected subsets of T cells, such as MHC class I and II, ... quantification of the CD1d protein bands indicated was performed using a Kodak Digital Science DC40 camera and its associated software Band intensities are shown on the right and are expressed as the sum of ... cells and molecules of the immunological system in hepatic injury and repair Understanding the interdependence between the metabolism of iron and lipids [14,56,57] may be relevant in a variety of...

Ngày tải lên: 19/02/2014, 16:20

... for optimal induction of the S100A8 gene in vitro and because of the potentiation exhibited by heparin, ECM heparan sulfate may be important FGFs bind heparin and heparan sulfates in positively ... reorganization of the phagocyte microtubular system [25] Expression of S100A8 in the absence of S100A9 in activated murine macrophages [26,27] and keratinocytes [22], and presence of S100A8 without ... FEBS Expression and regulation of S100A8 in fibroblasts TGF-b accelerates deposition, remodeling and maturation of collagen later in the healing process of dermal wounds [54,55] Differentiation of...

Ngày tải lên: 19/02/2014, 18:20

... switch and alters the expression of the two nuclear genome coded isoforms of CytOX V [10,32] The regulation of genes CytOX5a and CytOX5b, coding for the two isofoms Va and Vb, parallels that of genes ... different stages of adaptive response to hypoxia Based on the results of our study and those of Schumacker, it is reasonable to conclude that the effects of hypoxia on CytOX gene expression and its activity ... co-expressed under hypoxic (O2 < 0.5 lM) and heme deficient conditions [11] The coexpression of specific subunit V and cytochrome c isoforms indicates that these isoform pairs act synergistically to...

Ngày tải lên: 20/02/2014, 23:20