expression in response to external stimuli

... response to exogenous H2O2 169 41 GAD expression in response to NaCl 170 42 GAD expression in response to mannitol 172 43 GAD expression in response to exogenous glutamate 173 44 GAD expression in response ... GAD expression in response to exogenous 2,4-D 165 38 GAD expression in response to different growth regulators 167 39 GAD expression in response to exogenous paraquat 168 40 GAD expression in response ... ACC 1-aminocyclopropane-1-carboxylic acid AG amino-guandine AS acetosyringone AVG 2-aminoethoxyvinyl glycine BA Benzyladenine BCIP 5-bromo-4-chloro-2-indolyl-phosphate CHA cyclohexylamine DAB...

Ngày tải lên: 12/09/2015, 10:19

... method to join DNA: homopolymer tails Alternate method to join DNA: linkers Introduction of recombinant DNA into living cells via vectors • Autonomously replicating DNA molecules – (have an origin ... interest is divided into small pieces that are then placed into individual cells (usually bacterial) • These can then be separated as individual colonies on plates, and they can be screened to ... replicating DNA molecules • Frequently carry drug resistance genes • Can be present in MANY copies in the cell A common plasmid cloning vector: pUC lacZ mulitple cloning sites pUC ApR ColE1 origin...

Ngày tải lên: 13/03/2014, 18:39

... fragments can be inferred Using clone names, the contigs or singletons corresponding to the two ends of a given clone were joined together by adding 20 Ns in the middle of both sequences Since 20 is ... pigment-binding protein essential for regulation of photosynthetic light harvesting Nature 2000, 403(6768):391-395 Karin M: Mitogen-activated protein kinase cascades as regulators of stress responses ... used to find, in the same reading frame, start or stop codons that would define the start and end points of coding sequences and UTRs in the ESTs Molecular marker design We used the SSRFinder...

Ngày tải lên: 12/08/2014, 05:20

... usually seen within 72 hours after specimen inoculation Inclusions varied in size and staining but were generally much smaller, than usually seen in case of C pneumoniae infection in cultured cells ... current problems in laboratory diagnostics of chlamydial infection The protocol used in our study is hardly adjustable to routine work in regular diagnostic laboratory since it requires cultured ... for 72 hours at 37° C in 5% CO2 A 24 well plate rather than 96 well plates was used in the study to avoid potential cross contamination Each serum specimen inoculated into 24 well dish was followed...

Ngày tải lên: 26/10/2012, 08:57

... transformant in Luria–Bertani medium containing 50 lgÆmL)1 ampicillin was inoculated into 200 mL of M9YG medium [29] containing 50 lgÆmL)1 ampicillin The cells were grown at 37 °C to an A600 value ... of recombinant HRT proteins in E coli cells In order to obtain HRT gene products, the cDNAs were expressed in E coli by means of a pET32b(+) expression system that contains a thioredoxin fusion ... essentially according to the protocol of Xpress Protein Purification System (Invitrogen), using a Ni2+ nitrilotriacetic acid-agarose column The soluble proteins were applied to a Ni2+ resin column, and...

Ngày tải lên: 07/03/2014, 21:20

... Figure 1A shows a semithin section demonstrating the darkly stained foot mucous cells in the ectoderm of the foot Stained diaminobenzidine containing granules are concentrated in the apical part of ... the diaminobenzidine-stained granules in the foot mucous cells (fm), and granules without diaminobenzidine-staining as a control (D) Scale bars are mm in (A), 20 lm in (B), and 1.5 lm in (C) and ... amino acids between this chitin binding region and the modules of PPOD1 and PPOD2 are not considered to be essential for chitin binding The deduced protein sequences of ppod1 and ppod2 contain...

Ngày tải lên: 08/03/2014, 10:20

... rRNA contains a hairpin structure, although much smaller in size, that corresponds to the E coli S8 binding region However, no protein corresponding to S8 was found in mammalian mitoribosome ... Therefore, the C-terminal portion of MrpS8 might be critical for its binding to rRNA in yeast mitochondria as well, despite that the amino-acid sequence responsible for the binding in bacterial counterparts ... in a sharp contrast to the mrps similar to bacterial ribosomal proteins The mitoribosome-specific proteins may have functions other than being involved in the translation in mitochondria Otherwise,...

Ngày tải lên: 17/03/2014, 10:20

... that in BirA, the N-terminal domain is somehow involved in tight-binding of the two ligands In future, it would be interesting to study a BPL:BirA chimera by fusing the DNA-binding domain at ... provides insight into how the N-terminal DNA-binding domain interacts with the 40 bp biotin operator sequence [12] The structure of the apo- and holo-forms of the biotinylation domain of E coli ... (which also includes M tuberculosis) which lack the N-terminal DNA-binding domain found in E coli BirA [19] In E coli, we have expressed active A aeolicus BPL, the biotin-binding domain of A aeolicus...

Ngày tải lên: 31/03/2014, 07:20

... including residues 12±17 [13] Name Inactive or weakly active Rubellidin 3.1a Trytophyllin L3.1b Electrin 2.1c Caeridin 3d Maculatin 1.3e Inhibitor Group Lesueurin Aurein 1.1 Citropin 1.1 Aurein ... lesueurin as the original nNOS inhibitor found, citropin 1.1, another member of inhibitor group 1, frenatin (inhibitor group 2) and caerin 1.9 (inhibitor group 3) The fact that the regression lines ... ability to inhibit calcineurin, another calmodulin dependent enzyme Lesueurin at 74 lM (4.6-fold greater than the IC50 against nNOS) inhibited calcineurin by 33% Citropin 1.1 reduced calcineurin activity...

Ngày tải lên: 31/03/2014, 15:20

... locations adjoining equipment and in equipment rooms Equipment Location Min Defl., in (mm) Min Defl., in (mm) 20 to 30 ft (6 - m) Floor Span 30 to 40 ft (9-12 m) Floor Span Min Defl., in (mm) Reference ... locations adjoining equipment and in equipment rooms Equipment Location Min Defl., in (mm) Min Defl., in (mm) 20 to 30 ft (6 - m) Floor Span 30 to 40 ft (9-12 m) Floor Span Min Defl., in (mm) Reference ... locations adjoining equipment and in equipment rooms Equipment Location Min Defl., in (mm) Min Defl., in (mm) 20 to 30 ft (6 - m) Floor Span 30 to 40 ft (9-12 m) Floor Span Min Defl., in (mm) Reference...

Ngày tải lên: 31/03/2014, 18:20

... carries the genetic information from the nucleus to the protein-synthesizing machinery in the cytoplasm Then, mRNA is translated into the sequence of amino acids that make up a protein The basis of ... a guanine, every guanine by a cytosine, every adenine by a thymine, and every thymine by an adenine In this way, information in DNA is correctly transcribed into RNA 1.2 Probe Hybridization Another ... lines, (2) increasing the concentrations of relevant RNAs by drug-induced overexpression of genes of interest, and (3) inhibition of protein synthesis by inhibitors, resulting in extended transcription...

Ngày tải lên: 11/04/2014, 01:04

... the financial value in Slovak crowns of timber reserve of the main stand and secondary crop as well as total production V (SKK) in dependence on their site index SI and age t according to the ... production at the age of 35 years Remaining 85–75% is the proportion of the main stand Total current and total mean increments were derived from total production Looking at Figs and we can state that ... direct or indirect costs, can increase their net Fig I-214 – the net yield of total current increment (TCI) Age (years) yields in timber production in poplar stands very and total mean increment...

Ngày tải lên: 07/08/2014, 03:22

... dates, toward the end of the growing season, there was an extreme increase in the inoculum amount in the environment and a significant increase in infection pressure, which caused a partial blurring ... intensity of infection during the growing season in 2005 and 2007 was evaluated in the same way The ranking of clones according to rust incidence was also similar in this case In 2005 the coefficient ... due to changes in the pathotype spectrum (L et al 1994; D, B 2007) In ‘Luisa Avanzo’ and in ‘Beaupré’ the loss of resistance to M larici-populina was sustained in response to...

Ngày tải lên: 07/08/2014, 10:21

... have minimal diameter 20 cm Class C3 is intended mainly for the pulp industry and class D1 includes fuel wood The volume of larger decay or cavity, mainly in lower parts of stems, was included in ... remained in fact the same also in the amended standard of 2007 Diameter classes 1–6+ are defined according to the mean diameter of logs without bark Quality of stems was evaluated on standing ... diameter degrees, N – total probability Parameter A indicates the position or more exactly it determines the minimal diameter and beginning of distribution Although parameter B indicates the scale...

Ngày tải lên: 07/08/2014, 10:21

... inoculated on CEF cells of different genetic type to examine the interactions between virusspecific cell receptor and virus envelope glycoproteins Two isolates replicated in line CEF cells, indicating ... cycles at 92℃ for 1min, 48℃ for 1min, and 72℃ for 1min A final extension was conducted at 72℃ for 5min PCR products were resolved in 1.5% agarose gel with electrophoresis in tris-acetate-EDTA ... to all ALVs) 4Line CEF; C/E CEF (resistant to infection with endogenous ALV) 5alv6; C/AE CEF (resistant to infection with subgroup A and E ALV) Isolation of Subgroup J Avian Leukosis Virus in...

Ngày tải lên: 07/08/2014, 15:20

... probiotic strains As considered with BSH activity, and in vitro cholesterol-lowering test, the final probiotic strains were in Streptococcus strains, 11 in Lactobacillus strains and in Bifidobacterium ... Streptococcus strains (11/18 strains) and 71.8% in Lactobacillus (28/39 strains) were also tolerant in pH 2.5 In case of Bifidobacterium, 27.9% (12/43 strains) were tolerant in pH 3.0 In addition, Bifidobacterium ... to pH 2.5 (for Streptococcus and Lactobacillus) and pH 3.0 (for Bifidobacterium), respectively, by using N HCl, and put into ml per a ml vial Streptococcus and Lactobacillus were inoculated into...

Ngày tải lên: 07/08/2014, 18:20

... planting should contain approximately the same number of plant clone Ex vitro rooting was applied in order to simplify the protocol and to reduce production costs The advantages are that the rooting ... which are being field trialed are maintained in our lab by repeated cold storage cycles (4 yr at 4°C + normal subcultures at 25°C) until results are available In vitro can or (Meier-Dinkel, Moreover, ... (Quercus robur L) and Linden (Tilia cordata Mill) Biol Plant 26, 374-377 Chalupa V (1988) Large scale micropropagation of Quercus robur L using adenine-type cytokinins and thidiazuron to stimulate shoot...

Ngày tải lên: 08/08/2014, 19:21

... considering the 3rd to the 7th individuals of those rows (the 2nd to the 4th individuals at Orsay) = = Size inequality was measured by means of the coefficient of variation (CV) and the Gini index ... hardwood cuttings were in April 1987, after being submerged in water for 48 h in complete darkness The criteria for the selection of the cultivars were disease resistance, photoperiodic response, ... places of origin, productivity range, parentage) were given in Ceulemans et al (1984) Eighty-one cuttings per clone were set out in a x square planting pattern with a single spacing of 0.8 x 0.8...

Ngày tải lên: 08/08/2014, 19:21

... will be integrated with the information on crown architecture and leaf area distribution, in order to provide a stronger basis for understanding and optimizing yields in SRIC of Populus This information ... branches in clone 111-005 The ratio of total LA on sylleptic branches to LA on proleptic branches was lower in the hybrid clones when compared to the parental species, early and late in the growing ... its branchiness index (i.e., the ratio of total branch DW to total stem DW) was highest as compared to the other clones Branch length and branch diameter were highly correlated within all clones,...

Ngày tải lên: 09/08/2014, 02:21

... been mapped to BTA29 using the INRA somatic hybrid cell panel [11] The panel of BACs obtained in this study constitutes an essential tool to solve the remaining ambiguities of the bovine karyotype ... directly using a Dye Terminator kit (Perkin Elmer) For IGH@, the PCR product was cloned in the vector PGEMT (Promega) and sequenced with a universal sequencing kit (Perkin Elmer) The sequencing products ... genes and were used to assign the corresponding genes by analysis on the INRA hamster-bovine somatic cell hybrid panel [11] and to screen a bovine BAC library to obtain corresponding BAC clones MATERIALS...

Ngày tải lên: 09/08/2014, 18:21