expressed sequence tag est database

... alternative approach by functional analysis of heterologously expressed P450 based on genomic sequences or expressed sequence tags (EST) appeared 22 O OH 2,3-Oxidosqualene HO Sophoradiol OH HO ... (5¢-AAACACTAGTATGCTAGACATCAAAGG CTAC-3¢, and 5¢-TTCAATCGATTCAGGCAGCGAACG GAGTGAA-3¢), which were synthesized based on the reported sequences The obtained clone was sequenced in both strands This sequence ... reported sequences [30] and RNA prepared from yeast extract-treated cell cultures of G max (data not shown) The genes obtained were ligated into expression vector pYES2 (Invitrogen) and expressed...

Ngày tải lên: 19/02/2014, 07:20

... laborious task [5] Expressed sequence tags (ESTs) are a less expensive alternative for gaining information about the expressed genes of an organism [6] In particular, the ESTs from a full-length ... sequencing of expressed sequence tags Genes & genetic systems 2004, 79(4):227-232 Forment J, Gilabert F, Robles A, Conejero V, Nuez F, Blanca JM: EST2 uni: an open, parallel tool for automated EST analysis ... Oger-Desfeux C, Marais G, Widmer A: A White Campion (Silene latifolia) floral expressed sequence tag (EST) library: annotation, ESTSSR characterization, transferability, and utility for comparative mapping...

Ngày tải lên: 12/08/2014, 03:21

... The Ambystoma mexicanum EST database The Ambystoma mexicanum EST database A relational database was created as a sequence storage and annotation resource of the sequenced ESTs from A mexicanum ... p27Kip1 gene from the EST sequence Two EST sequences of the p27Kip1 gene were sequenced in the EST collection but neither were full-length sequences To isolate the full-length sequence, 200,000 clones ... potential full-length sequences Forty percent of our EST sequences did not generate a significant hit in the non-redundant protein database The availability of additional sequence databases including...

Ngày tải lên: 14/08/2014, 14:21

... nhƣ NCBI, EMBL, DDBj… Một CSDL lớn ESTs (Expressed Sequence Tags), có ESTs chi cam chanh (citrus) Những trình tự ESTs đƣợc sử dụng để khai thác SSRs (Simple Sequence Repeats) Những SSRs hữu ích ... from ESTs is inexpensive comparing to the traditional methods Methodology 1) We used perl script to receive EST sequences from database NCBI 2) Finded and separated SSRs include in ESTs database ... contain database control software to share information with users Results: 28,241 SSR-containing ESTs (EST- SSRs) were identified by analyzing 191,110 ESTs sequences belonging to Citrus in dbEST division...

Ngày tải lên: 03/09/2013, 10:00

... Transactions on Database Systems, Vol 29, No 2, June 2004 Expressing and Optimizing Sequence Queries in Database Systems • 289 SELECT SessNo, count(*A) FROM Sessions CLUSTER BY SessNO SEQUENCE BY ... Sessions CLUSTER BY SessNO SEQUENCE BY ClickTime AS (*X, Y) ACM Transactions on Database Systems, Vol 29, No 2, June 2004 Expressing and Optimizing Sequence Queries in Database Systems • 309 WHERE ... Very Large Databases 64–72 SADRI, R 2001 Optimization of sequence queries in database systems Ph.D thesis, University of California, Los Angeles SESHADRI, P 1998 Predator: A resource for database...

Ngày tải lên: 30/03/2014, 22:20

... degree of sequence similarity to each of the three selected databases Sequences showing similarity to only one database are not shown Sequences showing sequence similarity to only two databases ... Proportion of ESTs predicted to encode signal sequences Proportion of ESTs predicted to encode signal sequences (a) EST sequences were classified as conserved (similarities to non-nematode database ... been by expressed sequence tag (EST) projects [36] Large-scale EST sequencing of the human filarial parasite Brugia malayi [37,38] has been followed by similar studies in the sheep intestinal...

Ngày tải lên: 09/08/2014, 20:20

... available DNA sequence data In particular, the expressed sequence tags (ESTs) provide a valuable resource for identifying and developing gene-associated SSR markers Linkage of EST- SSR markers ... methodology [44] Castor bean EST sequences were obtained via the ENTREZ search tool of the EST database at the NCBI http://www.ncbi.nlm.nih.gov/nucest A total of 62,611 castor bean ESTs originated from ... sequence repeat (SSR) markers from wheat expressed sequence tags (ESTs) Theor Appl Genet 2004, 109:800-805 25 Kota R, Varshney RK, Thiel T, Dehmer KJ, Graner A: Generation and comparison of EST- derived...

Ngày tải lên: 11/08/2014, 11:21

... remove pGEMT-Easy vector sequences A Perl script EST trimmer [12] was used to trim adaptors, poly A/T ends EST sequences which were less than 100 bp long were removed Manual sequence processing was ... with all chickpea EST sequences (34,587) deposited in NCBI dbESTs identified 1576 unigenes (51.4% of total unigenes) as singlets and are new entries to the chickpea database ESTs from forward ... determine sequence homology at the nucleotide level of this unigenes set with EST databases of Medicago truncatula, Glycine max, Lotus japonicus, and Phaseous vulgaris and also with ESTs of model...

Ngày tải lên: 11/08/2014, 11:22

... several ESTs that make up the 3’ end, again justifying the creation of a separate contig Contigs #2 and contain the greatest number of ESTs, 34 and 27, respectively, suggesting that these sequences ... a perfect match with a sequence reported previously Comparison of Butte 86 gamma gliadin contigs with contigs from other EST assemblies A number of EST databases include sequences from Butte 86 ... contigs in TaGI Release 10.0 ESTs from Butte 86 contigs #5, and were found in two TaGI contigs while ESTs from contig #1 were distributed among three TaGI contigs ESTs from only five Butte 86...

Ngày tải lên: 12/08/2014, 03:21

... Cichorium intybus ESTs Total ESTs ESTs from the E library ESTs from the NE library Average sequence size (bp) Average sequence size of annotated ESTs (bp) Average sequence size of ESTs without match ... cellspecific transcripts by expressed sequence tag database analysis Fertil Steril 2001, 76(3):550-554 Miner D, Rajkovic A: Identification of expressed sequence tags preferentially expressed in human ... classification of ESTs Annotation of the ESTs using BlastX searches against sequences in NR GeneBank database, revealed that 55% of the ESTs had a high similarity with the best match (Evalue...

Ngày tải lên: 12/08/2014, 05:20

... that vector sequences were not included in EST sequences Sequence data analysis and EST clustering The 5' DNA EST sequence chromatogram data were basecalled using the program Phred [10]; EST reads ... specific cDNAs from 4,720 EST sequences We found that the castor oleate desaturase (RcFAD2) sequence is much less abundant than that of the FAH12 in our cDNA sequences, suggesting a transcriptional ... assembly of these sequences resulted in a total of 1,908 unique EST sequences with 587 contigs (30.8%) (Fig 2) and 1,321 singletons (69.2%) We have deposited 4,288 sequences in the dbEST division...

Ngày tải lên: 12/08/2014, 05:20

... Finally, several TCs – Cl000209:1 (61 ESTs) Cl000582:1 (18 ESTs) Cl001827:1 (5 ESTs) and Cl000731(2 ESTs) – show similarity to potyviral sequences, indicating that the sequenced library likely derives ... The complete set of raw EST sequences, as well as of their electopherograms, are maintained in the database allowing users investigate on library qualities and on single EST structural features ... of sequences Avg EST length (nt) Nucleotides masked 1,376 239 10.2% ESTs in contigs The EST dataset was subjected to a clustering/assembling procedure [5], in order to group ESTs putatively derived...

Ngày tải lên: 12/08/2014, 05:20

... immunotherapy 38 1.9 Expressed Sequence Tagging - powerful approach for genome investigations 43 1.10 Objectives of the study 48 Chapter 2: Materials and methods 2.1 Expressed Sequence Tagging (EST) approach ... tropicalis in the tropics Expressed Sequence Tagging (EST) is a useful approach for genome level transcript profiling EST database of D farinae was generated and the database generated was compared ... Biological function assignments of ESTs based on sequence homology 89 3.1.9 EST as tool for rapid identification and characterization of dust mite genes 92 3.2 EST databases aid in dust mite allergy...

Ngày tải lên: 12/09/2015, 11:08

... stage Adult Adult Adult Adult 1 Adult Adult Adult Egg Adult Lung stage Adult Lung stage Egg Egg Lung stage Lung stage Egg Adult Egg Lung stage Adult Adult Adult Adult Adult Adult Adult Lung stage ... redundant databases using BLAST programs and elim- analyzed All of them were shown to be unidirectional ination of ESTs corresponding to useless sequences (vec- (most ESTs had matches to database sequences ... significance of the alignment, ESTs were named as putative identification for the gene ESTs with no significant database matches or showing only partial homology with database sequences were grouped...

Ngày tải lên: 11/11/2016, 15:59

... (no ESTs); GB (EST) , GenBank dbEST; PIR, Protein Information Resource; SP, SwissPROT; TREMBL, translated from EMBL Expressed Sequence Tags from the Black Tiger Shrimp 473 Table Microsatellite sequences ... types within the eyestalk The most common sequence homology identified among the eyestalk ESTs was to arrestin (AIMS-P.mon10, AIMS-P.mon18, AIMS-P.mon31, AIMS-P.mon37) Arrestins are a family ... and Waterston, R.H (1998) Expressed sequence tags—ESTablishing bridges between genomes Trends Genet 14:4–7 Matsumoto, H., and Yamada, T (1991) Phosrestins I and II: arrestin homologs which undergo...

Ngày tải lên: 11/11/2016, 15:59

... Ordering Domain who are primarily interested in sequencedata, for example, to directly query named sequences without having to embed Figure 1: Data Sequence the sequences inside relational tuples ... enhancementsare sequencesand relations The important questionto askis: how doesthe interaction betweensequences and relations occur? The answer is difficult to explain with meaningful examples at this stage ... difficult to control various properties of eachsequence.The propertiesof interest in eachsequence are: (1) the cardinulity, i.e., the number of records in the sequence, (2) the record width, i.e., the...

Ngày tải lên: 16/03/2014, 16:20

... 5v5 experiment using t-test Using GEO variances on a 2v2 test returns 231% more of the top 50 genes than the unadjusted t-test While we are not suggesting that a simple t-test is a recommended method ... a regularized t-test By smoothing out the variance estimates, the regularized t-test returns a more accurate assessment of differentially expressed genes than the standard t-test Thus the gains ... out the effects of underestimated variances and therefore returns a more reliable assessment of differentially expressed genes in small samples than the standard t-test Testing procedure and dataset...

Ngày tải lên: 14/08/2014, 14:21

... dùng nhấn vào nút này, sau import thành công hiển thị liệu lên DataGridView o DataGridView: tên dgvData, để chứa liệu lấy từ database sau import xong o o Lable “File Path” tùy ý Tham khảo hình ... cài máy hành bạn), chọn database tên “HumanResourceDB” (database tạo sẵn bước 2) -> nhấn nút OK -> Next, làm theo dẫn wizard, xem hình bên dưới: • Hình 5: tạo kết nối với database Các chủ đề tương ... DataTable data = ReadDataFromExcelFile(); // Import liệu đọc vào database ImportIntoDatabase(data); // Lấy hết liệu import từ database hiển thị lên gridView ShowData(); } private bool ValidInput()...

Ngày tải lên: 18/08/2012, 11:53

... liệu bảng cha bảng có foreign key giá trị primary key bảng cha xóa chọn: a b Delete c Cascade d Restrict 20 e Nullifiles 21 Có ràng buộc quy định giá trị cột mã sinh viên là: XXX999XXXX , ràng ... SALES information, sorted by SALES_PERSON, from A to Z, and SALES_DATE, from most recent to earliest? • A SELECT invoice_no, sales_person, sales_date, sales_amt FROM sales SORT BY sales_person,...

Ngày tải lên: 18/08/2012, 11:53

... dụng Database- Specific Integrity Rules nghiêm chỉnh database bị hư không dùng Microsoft Access Database Management System (MSAccess DBMS) Microsoft Access Database Management System gồm có Database ... mà bạn muốn dùng để nhét vào Access database mở: Database Server số ý niệm Dù Jet Database Engine relational database tốt hiệu năng, thuộc loại File Based database, tức thụ động, không chạy phải ... Table Titles có Primary Key ISBN Relational Database Một database có nhiều tables hổ trợ liên hệ, one-to-many, gọi Relational Database Khi thiết kế database, ta tìm cách đặt liệu từ giới thật...

Ngày tải lên: 22/08/2012, 11:03