export translation and post translational processing

... microtubules and, accordingly, it is highly concentrated in growing axons CRMP-2 binds to tubulin heterodimers, and its overexpression in neurons promotes axonal growth and branching [6] Extensive post- translational ... relationships between the folding and denaturation behaviour of CRMP-2 4590 and neuronal fibril formation in Alzheimer’s disease brains Post- translational modifications and previously characterized ... growth cone guidance, and its function is likely to involve both homo- and heterotetramerization, as well as post- translational modifications Using a number of biochemical and biophysical methods,...

Ngày tải lên: 16/03/2014, 06:20

... Relieving translational repression may lead to apparent translational upregulation, because investigators [8,9] showed that treatment with antifolate drugs can reduce the otherwise detectable translational ... several members of the P falciparum pentose phosphate and REDOX metabolic pathways [12,13] Besides translational control of protein expression, posttranslational modifications (PTMs) have also been ... electrophoresis intensity and level off for the rest of the time course, and two isoforms (4 and 5) that undergo a significant decrease through TP1 and TP2 and subsequent recovery in TP3 and TP4 (Figure...

Ngày tải lên: 14/08/2014, 21:20

... equipment and software loans, guidance, and advice: Dr X Charlie Dong and lab members, Dr Timothy Corson and Kamakshi Shishtla, and Dr Nuria Morral and lab members  To my parents, Jack and Shirley: ... hyperphosphorylation of the CTD and RNAPII turnover and proteolytic degradation as well as post- translational modifications of the globular core of RNAPII Following tandem affinity purification, ... protein-protein interaction and potential post- translational modifications Multidimensional protein identification technology (MuDPIT) was developed in 2001 by Washburn and colleagues and couples two methods...

Ngày tải lên: 24/08/2014, 09:44

... Coomassie stained and major bands were excised and eluted in order to perform N-terminal sequencing Tandem affinity purification and mass spectrometric analysis of USP7 N-terminal and C-terminal TAP-tagged ... expression vectors and USP7 cDNA as well as Shirley Gil-Parrado, Bruno Martoglio, Martin Renatus and Jorg Eder (Novartis, Basel, Switzerland) ¨ ´ for support and helpful discussions A Fernandez´ Montalvan ... 999.5 were found for USP7 1-560, corresponding likewise to acetylated and single phosphorylated USP7 1-560, respectively This post- translational modification was later confirmed in USP7 purified from...

Ngày tải lên: 07/03/2014, 05:20

... activity (data not shown), but it may bind and sequester post- translationally modified OTUB1 and GDP bound RhoA to interfere with active RhoA formation, and perhaps provoke premature degradation ... and OTUB1–probe adduct were visualized by immunoblotting using HA antibodies and quantified for two experiments (black and grey bars) The intensities of the corresponding bands were measured and ... YpkA and RhoA interact in vitro and whether this protein complex includes OTUB1 YpkA was immunoprecipitated and the presence of OTUB1 and RhoA examined by immunoblotting (Fig 6C) YpkA, OTUB1 and...

Ngày tải lên: 16/02/2014, 15:20

... Grundke-Iqbal I & Iqbal K (2005) Post- translational modifications of tau protein in Alzheimer’s disease J Neural Transm 112, 813–838 56 Huq M, Gupta P & Wei LN (2008) Post- translational modifications ... Characterization of posttranslational modifications of the linker histones H1 and H5 from chicken erythrocytes using mass spectrometry J Proteome Res 7, 4326–4335 Ficarro SB, McCleland ML, Stukenberg ... histone variants, and their post- translationally modified forms Electrophoresis 29, 2516–2532 67 Mizzen CA, Dou Y, Liu Y, Cook RG, Gorovsky MA & Allis CD (1999) Identification and mutation of phosphorylation...

Ngày tải lên: 18/02/2014, 08:20

... gland – Venom gland – Ganglia ⁄ heart Muscle Heart Venom duct Venom gland Sinus gland – spider [6] and cone snails [7] l- to d-aminoacyl isomerization, or epimerization, comprises a true posttranslational ... BM (2005) Conotoxins and the posttranslational modification of secreted gene products Cell Mol Life Sci 62, 3067–3079 Kreil G (1994) Conversion of L- to D-amino acids: a posttranslational reaction ... chain in terms of substrate specificity and availability Results Purification and characterization of VIH I and II VIHs were purified from H americanus sinus gland extract by RP-HPLC Figure shows a...

Ngày tải lên: 18/02/2014, 11:20

... barkeri, and MCR from Methanopyrus kandleri [7] In case of the enzyme from Methanothermobacter marburgensis and Methanosarcina barkeri, the resolution was high enough to identify the post- translational ... or growth conditions An early hypothesis was that the post- translational or cotranslational modifications modulate the kinetic properties (Km and Vmax) of MCR, which is why we started the analysis ... nickel cofactor F430 [16] and for the post- translational modifications [9] are not yet known [17,18] Also, the in vitro reconstitution of active enzyme from its subunits and cofactor has proven...

Ngày tải lên: 16/03/2014, 05:20

... Segmented Translation Model (b) Post- Processing Model Translation & Generation Figure 1: Training and testing pipelines for the SMT models is to take the abstract suffix tag sequence y ∗ and then ... Conclusion and Future Work We found that using a segmented translation model based on unsupervised morphology induction and a model that combined morpheme segments in the translation model with a postprocessing ... statistical machine translation In Proceedings of Machine Translation Summit X, pages 79–86, Phuket, Thailand Association for Computational Linguistics John Lafferty, Andrew McCallum, and Fernando Pereira...

Ngày tải lên: 30/03/2014, 21:20

... contain more agerelated post- translational modifications in the form of the isomerized amino acid IsoAspartate and (b) synovial fluid levels of the age-related protein post- translational modification ... design and the manuscript VBK conceived and designed the study, supervised the project, and assisted in both the statistical analysis and the manuscript preparation and editing All authors read and ... JBC and DB contributed equally to the experimental design, the preparation of the manuscript, and the statistical analysis MB and RDZ coordinated and organized the primary sample collection and...

Ngày tải lên: 09/08/2014, 14:20

... H2A, H2B, H3 and H4 [4] An additional histone, H1 links these nucleosomes together along the chromatin chain In general, the N terminus of histone H3 and H4, and N and C terminus of H2A and H2B are ... immunoprecipitation (ChIP-chip), and those distribution patterns are consistent with their functions [19] Posttranslational modifications (PTMs) can regulate the plant's responses to internal and external signals, ... (H3.1 and H3.2) and histone H4 (H4.1 and H4.2) are also identified and different modifications of the two variants of histone H3 are also studied Results Although histone modifications and their...

Ngày tải lên: 12/08/2014, 03:21

... chromatin-binding proteins Suv39h1 and G9a Post- translational modifications of histones H3 and H4 associated with the Post- translational modifications of histones H3 and H4 associated with the chromatin-binding ... 2007, Volume 8, Issue 12, Article R270 Robin Post- translational modifications of Suv39h1- and G9aassociated histones H3 and H4 We have studied post- translational modifications of chromatin-bound ... H3 and H4 associated with Suv39h1 or G9a proteins (d) Posttranslational modifications of histone H4 associated with Suv39h1 or G9a Shown are the means of three independent experiments (± standard...

Ngày tải lên: 14/08/2014, 08:20

... Archimedes’ works 1 10 Translation and Commentary On the Sphere and the Cylinder, Book I On the Sphere and the Cylinder, Book II Eutocius’ Commentary to On the Sphere and the Cylinder I Eutocius’ ... main results of Propositions 23 and following (“chapters 5–6”) SC II, on the other hand, has a few theorems (Propositions 2, 8, and 9) but at least 2, and possibly and as well, are there for the ... pedagogic in nature Postulates 1–2: about what? The wording of the translation of Postulate gives rise to a question of translation of significant logical consequences My translation has “ among...

Ngày tải lên: 21/09/2012, 11:00

... determine the exact moment when to pick a crop , we have to consider the idea of maturity  The postharvest behavior of a commodity depends on the stage of maturity when it was harvested  Maturity...

Ngày tải lên: 03/04/2013, 20:55

... Vector Analysis Matiur Rahman and Isaac Mulolani Continuous Signals and Systems with MATLAB® Taan ElAli and Mohammad A Karim Discrete Systems and Digital Signal Processing with MATLAB® Taan ElAli ... Introduction to Engineering and Computer Science with C and MATLAB and Continuous Signals and Systems with MATLAB Dr ElAli has contributed a chapter to The Engineering Handbook published by CRC ... father Saeed and mother Shandokha May Allah have mercy on their souls It is dedicated then to my wife Salam; my beloved children, Nusayba, Ali and Zayd; my brothers, Mohammad and Khaled; and my sisters,...

Ngày tải lên: 01/01/2014, 21:13

... nuclear encoded protein is post- translationally targeted to mitochondria Identical results were obtained using a C-terminally His-tagged version of MDL1 (data not shown) Post- translational maturation ... mitochondria post- translationally [39] MTS usually consists of 20–35 residues and is highly degenerated in primary sequence, but is rich in basic, hydrophobic and hydroxylated residues and FEBS Journal ... cotranslational endoplasmic reticulum targeting to posttranslational mitochondrial import Mol Biol Cell 16, 1788–1799 40 Graf SA, Haigh SE, Corson ED & Shirihai OS (2004) Targeting, import, and...

Ngày tải lên: 18/02/2014, 16:20

... post- translationally modified protein mimics has also been used to explore dynamic regulatory PTMs such as tyrosine phosphorylation [65,66] and glutathionation [67,68] In all cases, the post- translationally ... of protein function by O-GlcNAc: a nuclear and cytoplasmic carbohydrate posttranslational modification Curr Opin Chem Biol 6, 851–857 Exploring post- translational modification 76 Lefebvre T, Guinez ... (2005) Protein posttranslational modifications: the chemistry of proteome diversifications Angew Chemie Int Edn 44, 7342–7372 Wold F (1981) In vivo chemical modification of proteins (post- translational...

Ngày tải lên: 18/02/2014, 17:20

... extracted in 160 · 160 pixel boxes and later binned by a factor of two Raw images ( 11 000) were band-pass filtered (20 to 1.5 nm) and masked and then iteratively aligned and classified using routines ... the case of the cyanide dihydratases, and also leads to definite suggestions about the interacting residues Examination of the complexes formed prior to post- translational cleavage suggests that ... oligomerization-dependent activation and may enable control of their oligomeric state in the industrial situation Here, we report the discovery of a specific post- translational cleavage of recombinantly...

Ngày tải lên: 19/02/2014, 00:20

... for distinguishing human translations from machine translations • Distinguish human and machine translations • Distinguish fluent machine translations from poor machine translations • Distinguish ... machine translations from human translations, but also further our understanding of fluency by providing comprehensive analysis of the association between fluency assessments of translations and surface ... fluency scores among machine translations and 300 randomly chosen human translations We then tried the classification task of distinguishing human and machine translations with different fluency quality...

Ngày tải lên: 22/02/2014, 02:20

... sense; purported translations, anyway, of all possible native sentences Translation between kindred languages, e.g., Frisian and English, is aided by resemblance of cognate word forms Translation ... analysis of radical translation The analysis is put forward in support of a general conclusion concerning the process of translation That conclusion, Quine's doctrine of translational indeterminacy, ... allow our rough -and- ready everyday understanding of the term "stimulation" to carry us along as we discover the manner in which Quine understands the relation of prompting If, as I stand beside a...

Ngày tải lên: 07/03/2014, 18:20