... hypoxantine, guanosine and guanine, uridine and uracil, cytidine and cytosine were 12.2 and 6.2 min, 10.5 and 4.7 min, 15.2 and 6.1 min, 6.8 and 4.2 and 6.6 and 3.9 respectively The amount of purine ... employed and the elution was carried out with : 94 (v v) mixture of 95% methanol and 0.1% triuoroacetic acid in H2O The retention times of adenosine and adenine, inosine and hypoxantine, guanosine and ... A, we were able to calculate the volume of buried and surface cavities for SsCUNH and the template, both in the monomer and in the tetramer, and we found that the volume of buried cavities found...
Ngày tải lên: 18/02/2014, 17:20
... unique features; and (d) to correlate catalytic behaviour of the enzyme with the specific features of its three-dimensional structure and evolution Results Production, purification and characterization ... Table Inhibition constants (Ki) and the type of inhibition of the PoHex from Penicillium oxalicum strains CCF 1959 and 3438 for GlcNAc and GalNAc reaction products, and comparison with the Hex from ... positions of N-formyl modified (light blue) and standard (magenta) substrates after ns of MD (C) Overlay of positions of N-propionyl modified (light blue) and standard (magenta) substrates after ns of...
Ngày tải lên: 14/03/2014, 23:20
Báo cáo khoa học: Characterization and structural modeling of a new type of thermostable esterase from Thermotoga maritima ppt
... schematic structural model consists of six a-helixes and has one central b-sheet made up of six b-strand-strands (Fig 3A) The first and second b-strand of the a ⁄ bhydrolase fold have not been modeled ... enzymes and consensus sequences Consensus sequences of the different lipase and esterase families [35, 36] and the two enzymes discussed in the text, PhaZ7 [28] and Est30 [20] are indicated understanding ... and the residual activities were measured using the standard method Stability against organic solvents and detergents was measured in the presence of 1% solvents and detergents within the standard...
Ngày tải lên: 23/03/2014, 09:20
Báo cáo Y học: The cold-active lipase of Pseudomonas fragi Heterologous expression, biochemical characterization and molecular modeling pdf
... peptide-independent pathway and calls for further investigation on this subject Enzyme activity and specificity In the standard tricaprylin hydrolysis assay, rPFL displayed highest activity at 29 °C and pH 8.0, ... substrate binding funnel (V123 with L119 and F249 with R224) and within the binding pocket where T18, F119, A247 and T251 in BCL are substituted in PFL by F, L, N and V residues, respectively Site-directed ... strain B11-1: gene cloning and enzyme purification and characterization Appl Environ Microbiol 64, 486–491 Feller, G., Thiry, M., Arpigny, J.L & Gerday, C (1991) Cloning and expression in Escherichia...
Ngày tải lên: 24/03/2014, 04:21
Two Phase Flow Phase Change and Numerical Modeling Part 1 pot
... advanced and revised contributions, and it covers mainly (1) numerical modeling of heat transfer, (2) two phase flow, (3) nanofluids, and (4) phase change The first section introduces numerical modeling ... Benta and Catalin Mihai Chapter Numerical Modeling and Experimentation on Evaporator Coils for Refrigeration in Dry and Frosting Operational Conditions 27 Zine Aidoun, Mohamed Ouzzane and Adlane ... Phase Flow, Phase Change and Numerical Modeling, Edited by Amimul Ahsan p cm ISBN 978-953-307-584-6 Contents Preface IX Part Numerical Modeling of Heat Transfer Chapter Modeling the Physical Phenomena...
Ngày tải lên: 19/06/2014, 12:20
Two Phase Flow Phase Change and Numerical Modeling Part 2 pdf
... experimentally determined and returned by the above presented method (Pearsica et al., 2010, 2008c) Fig Processing speed variation 24 Two Phase Flow, Phase Change and Numerical Modeling The experimental ... (2006) Investigation of Numerical Techniques with Comparison Between Anlytical and Explicit and Implicit Methods of Solving One- 26 Two Phase Flow, Phase Change and Numerical Modeling Dimensional ... temperature applications and novel modeling treatment of circuit design and frost deposition control 2.1 Modeling Modeling of refrigeration heat exchangers for design and performance prediction...
Ngày tải lên: 19/06/2014, 12:20
Two Phase Flow Phase Change and Numerical Modeling Part 3 doc
... water 62 Two Phase Flow, Phase Change and Numerical Modeling and refrigerant sides, in order to effectively shorten or reduce the time and cost in the development and design of this type of heat exchanger, ... [W/m2K], di and are, respectively, the inside and outside diameters of tubes 68 Two Phase Flow, Phase Change and Numerical Modeling [m], km is the thermal conductivity [W/mK], Rfi and Rfo are ... Phase Change and Numerical Modeling The present modeling procedures for determining the total heat transfer rate are shown as Fig and detailed as following steps: Input design parameters and geometry...
Ngày tải lên: 19/06/2014, 12:20
Two Phase Flow Phase Change and Numerical Modeling Part 4 pot
... capillary limit and the evaporator boiling limit An improved model is suggested and it is compared with the simulation and experimental Theoretical and Experimental Analysis of Flows and Heat Transfer ... size estimation, and performance rating of the shell -and- tube condenser has been developed, varified, and modified A good agreement is observed between the computed values and the experimental data ... refrigerant outlet temperature, and (c) heat transfer rate 81 82 Two Phase Flow, Phase Change and Numerical Modeling References Allen, B., & Gosselin, L (2008) Optimal geometry and flow arrangement for...
Ngày tải lên: 19/06/2014, 12:20
Two Phase Flow Phase Change and Numerical Modeling Part 5 pptx
... (Fig 21) 110 Evaporator Adiabatic zone Condenser 100 Experimental 10 W Experimental 20 W Experimental 30 W Experimental 40 W Experimental 50 W Experimental 60 W Model 90 T (°C) 80 70 60 50 40 30 ... 20 Variations of the FMHP wall temperature 180 160 Experimental 10 W Experimental 20 W Experimental 30 W Experimental 40 W Experimental 50 W Experimental 60 W Model 140 T (°C) 120 100 80 60 40 ... τlw = τ vw = Where kl and kv are the Poiseuille numbers, and Dhlw and Dhvw are the liquid-wall and the vapor-wall hydraulic diameters, respectively The hydraulic diameters and the shear stresses...
Ngày tải lên: 19/06/2014, 12:20
Two Phase Flow Phase Change and Numerical Modeling Part 6 pot
... attainment and maintenance of the plasma density and temperature on a required level and forces to develop sophisticated and expansive methods to feed and heat up the plasma components On the other hand, ... ∂rZ Numerical approaches elaborated to overcome problems arising on this way are presented and highlighted below on several examples 154 Two Phase Flow, Phase Change and Numerical Modeling Numerical ... Lines and depict the centreline and surface temperature, respectively Lines and illustrate the shell growth and solid fraction, respectively The borders of the final casting segments 5, 6, and...
Ngày tải lên: 19/06/2014, 12:20
Two Phase Flow Phase Change and Numerical Modeling Part 7 potx
... Nematollahi and Nazifi, 2007; Rodriguez and El-Genk, 2008a, b, c, and d; Rodriguez, Domino, and El-Genk, 2010; Rodriguez and El-Genk, 2010a and b; Rodriguez and El-Genk, 2011) There are many devices and ... and Γ ω are the effective diffusivity of k and ω, respectively; Gk and Gω are the generation of k and ω, respectively, due to mean velocity gradients; and Yk and Yω are the dissipation of k and ... azimuthal, (a) and (b), and axial, (c) and (d), velocity profiles for S2 configuration Q ≈ 100 l/h for (a) and (c); and Q ≈ 270 l/h for (b) and (d) The circles indicate mean velocity values and the error...
Ngày tải lên: 19/06/2014, 12:20
Two Phase Flow Phase Change and Numerical Modeling Part 8 ppt
... Goldstein and Behbahani, 1982; Kamotani and Greber, 1974; Kavsaoglu and Schetz, 1989; Kawai and Lele, 2007; Kiel et al., 2003; Patankar, Basu, and Alpay, 1977; Rivero, Ferre, and Giralt, 2001; Sucec and ... Applications for Enhanced Heat Transfer and Flow Mixing Fig 12 Impact of Re and θ on Azimuthal Velocity Field 203 204 Two Phase Flow, Phase Change and Numerical Modeling The single-jet computation ... Manual of Applied Mechanics, 9th Ed., C Griffin and Co., London, UK 216 Two Phase Flow, Phase Change and Numerical Modeling Rivero, A., Ferre, J A., and Giralt, F (2001) Organized Motions in a Jet...
Ngày tải lên: 19/06/2014, 12:20
Two Phase Flow Phase Change and Numerical Modeling Part 9 pot
... inlet temperature and exit quality To the aim, both basic experiments and numerical analyses are necessary This work is dedicated to the study (from theoretical, numerical and experimental point ... mono-wavelength case: for 1ω and 3ω (b) Evolution of the LID densities (expressed in dam./mm3 ) as a function of F3ω and F1ω The color levels stand for the experimental damage densities Modeling results ... Change and Numerical Modeling For pulsed laser ablation of metals, the ultrafast heating mechanisms perform great disparity for femtosecond and nanosecond pulse duration In fact, the electron and...
Ngày tải lên: 19/06/2014, 12:20
Two Phase Flow Phase Change and Numerical Modeling Part 10 doc
... Validation benchmark between analytical model and numerical models with RELAP5 and COMSOL codes Experimental campaign with helical coil tube geometry In order to experimentally study DWOs in helically ... section and approximation of the helical shape by assuming a straight channel long as the helical tube and with the same inclination of the helix 280 Two Phase Flow, Phase Change and Numerical Modeling ... Change and Numerical Modeling 10 Acknowledgments The Authors wish to thank Gustavo Cattadori, Andrea Achilli as well as all the staff of SIET labs for the high professionalism in the experimental...
Ngày tải lên: 19/06/2014, 12:20
Two Phase Flow Phase Change and Numerical Modeling Part 11 pdf
... ρv (9) where c and n were unknown beforehand, and then obtained using the experimental CHF data that c=0.386 and n=0.549, with the standard errors of 0.039 for c, 0.0154 for n, and 0.937 for the ... of low heat flux and surface superheat Pautsch and Shedd (2005) and Shedd and Pautsch (2005) conducted a series of spray cooling experiments with single and multiple nozzles and developed an ... with experimental data Comparisons of the surface temperature with the experimental data are listed in Table 2, and show the validity of the numerical model The deviation between simulation and experimental...
Ngày tải lên: 19/06/2014, 12:20
Two Phase Flow Phase Change and Numerical Modeling Part 12 pot
... Phase Change and Numerical Modeling ′ sin θ w nf = , sin θ f′ nw (8) where θ’w and θ’f are the angles of incidence and refraction, and x0 and y0 are the intersection points between laser and inner ... 324 Two Phase Flow, Phase Change and Numerical Modeling measurements of the apparent contact angle and the curvature of a drop and meniscus during condensation and evaporation processes in a constrained ... Two Phase Flow, Phase Change and Numerical Modeling cavity and get sucked in, resulting in a spectacular self-drying of the top surface (Narhe & Beysens, 2004), and/ or (ii) coalesce with another...
Ngày tải lên: 19/06/2014, 12:20
Two Phase Flow Phase Change and Numerical Modeling Part 13 docx
... 0.629 (Ca < 0.3, Re < 2000) , (21) 352 Two Phase Flow, Phase Change and Numerical Modeling where Ca = μU/σ and Re = ρUDh/μ and We = ρU2Dh/σ As capillary number approaches zero, Eq (21) should ... the water experiment is much smaller than those of other fluids and the 354 Two Phase Flow, Phase Change and Numerical Modeling numerical results It is confirmed that inertial effect must be considered ... restraint, the relation of Dh and δ0_corner is expressed as follows: 356 Two Phase Flow, Phase Change and Numerical Modeling 2 −1 ≥ Dh δ _ corner (27) From Eqs (23) and (27), the maximum value...
Ngày tải lên: 19/06/2014, 12:20
Two Phase Flow Phase Change and Numerical Modeling Part 14 pdf
... heated from horizontal and vertical sides and cooled from vertical side., Archives of Thermodynamics, Vol 31(2010), No 4, pp 51-59 386 Two Phase Flow, Phase Change and Numerical Modeling Bieliński, ... respectively Ue (s) and UVW (s), is sufficient to explain any tendency to the aggregation of the suspension This concept was developed by Derjaguin and Landau and also by Vervey and Overbeck In the ... in nanoparticulate form to the 390 Two Phase Flow, Phase Change and Numerical Modeling Will-be-set-by-IN-TECH fluid of interest and are named nanofluids, a term introduced by Choi in 1995 (Choi,...
Ngày tải lên: 19/06/2014, 12:20
Two Phase Flow Phase Change and Numerical Modeling Part 15 doc
... Hu, L W (2008) Experimental investigation of turbulent convective heat transfer and pressure loss of alumina/water and zirconia/water 434 Two Phase Flow, Phase Change and Numerical Modeling nanoparticle ... viscosity of the nanofluid (eff) and the base fluid (0), and Cμ and Ck are viscosity and thermal conductivity enhancement coefficients, determined from experimental viscosity and thermal conductivity ... were validated with wide range 424 Two Phase Flow, Phase Change and Numerical Modeling of experimental data under various conditions and thus are not widely accepted In an attempt to establish a...
Ngày tải lên: 19/06/2014, 12:20
Two Phase Flow Phase Change and Numerical Modeling Part 16 docx
... Microfluidics and Nanofluidics, 2010 8(2): p 145-170 448 Two Phase Flow, Phase Change and Numerical Modeling [28] Li, Y.J., et al., A review on development of nanofluid preparation and characterization ... Cheng, L.X., E.P Bandarra, and J.R Thome, Nanofluid Two-Phase Flow and Thermal Physics: A New Research Frontier of Nanotechnology and Its Challenges Journal of Nanoscience and Nanotechnology, ... [39] Chopkar, M., et al., Development and characterization of Al2Cu and Ag2Al nanoparticle dispersed water and ethylene glycol based nanofluid Materials Science and Engineering B-Solid State Materials...
Ngày tải lên: 19/06/2014, 12:20