exercise vitamin c and oxidative stress tissue injury summary

... transporter-2, and NOS in activated murine microglial cells (19) and co-induction of inducible NOS and arginine recycling enzymes in cytokine-stimulated PC12 cells and high output production of NO ... nitric oxide synthase (NOS; EC 1.14.13.39), and the citrulline generated as a by-product can be recycled to arginine by successive actions of argininosuccinate synthetase (AS; EC 6.3.4.5) and ... facilitate high and continuous production of NO and increased NO may decrease the activity of GS and increase the oxidative stress in anoxia/reperfusion 148 induced excitotoxicity Global hypobaric hypoxia...

Ngày tải lên: 26/10/2012, 09:07

... nonselective cation channels It is known that reduction of [Ca2+]e activates nonselective cation currents in hippocampal neurons [203] and neocortical nerve terminals [204] termed csNSC and NSC, respectively, ... noninactivating cation conductance upon reducing extracellular Ca2+ and Mg2+ in cultures of cortical neurons, as well as in cortical and hippocampal neurons in brain slices from adult mice, raising the ... intracellular calcium changes and neurotoxicity in cortical neurons in vitro: effect of chemical ischemia Neuroscience 62, 667–679 154 Hyrc K, Handran SD, Rothman SM & Goldberg MP (1997) Ionized intracellular...

Ngày tải lên: 07/03/2014, 12:20

... biosynthesis, 1-aminocyclopropane-1-carboxylic acid (ACC) synthase (ACS) and ACC oxidase (ACO), which catalyze the conversion of S-adenosyl-l-methione into ACC, and the oxidative cleavage of ACC to form ... transgenic tobacco plants Fig Analysis of ROS accumulation in wild-type and transgenic plants in response to abiotic and abiotic stresses (A) Virus infection-induced H2O2 accumulation detected by ... systemic acquired resistance Cell 103, 1111–1120 13 Xiong L & Yang Y (2003) Disease resistance and abiotic stress tolerance in rice are inversely modulated by an abscisic acid-inducible mitogen-activated...

Ngày tải lên: 14/03/2014, 23:20

... protein Obtaining a crystal structure of the calcium-bound form of Anx24(Ca32) proved extremely difficult, since the protein was hard to crystallize and did not bind calcium either by cocrystallization ... involved in redox reactions and might constitute the molecular basis of oxidative stress response by annexins Materials and methods Purification of recombinant protein Cloning and construction of an N-terminal ... mM CaCl2 for between h and day, in an attempt to obtain crystals of a calcium-bound form of Anx(Gh1) Also, cocrystallization was attempted with calcium concentrations of 2–15 mM in the presence...

Ngày tải lên: 17/03/2014, 03:20

... particular heart disease, hypertension and cataract Altered Na+ and K+ concentrations are observed in many forms of human cataract and correlate with increasing lens colour and with cortical opacification ... the chaperone-like a-crystallin Effect of a-crystallin on refolding of b- and c- crystallins J Biol Chem 270, 19888–19892 15 Wang, K & Spector, A (1995) a-Crystallin can act as a chaperone under conditions ... Sciences All other chemicals and enzymes, including luciferin–luciferase firefly lantern extracts were obtained from Sigma Sepharose 2B and Sephacryl S300 H were obtained from Pharmacia Ltd a-Crystallin...

Ngày tải lên: 17/03/2014, 03:20

... Alzheimer's disease: old and new mechanisms of action J Neurochem 2004, 91:521-536 Lim GP, Chu T, Yang F, Beech W, Frautschy SA, Cole GM: The curry spice curcumin reduces oxidative damage and amyloid pathology ... amyloid pathology characteristic of Tg models [24,25] Taken together, the data accumulated so far clearly indicate that oxidative imbalance and subsequent chronic oxidative stress are not only ... Effect of5 Effect of indomethacin plus vitamin E supplementation on amyloid deposition Percentage area of somatosensory cortex (SSC), hippocampus (HIP) and parahippocampal cortex (PHC) occupied...

Ngày tải lên: 19/06/2014, 22:20

... quantitative colorimetric sandwich ELISA (R&D Systems, Abingdon, UK) in accordance with the manufacturer's instructions Concentrations were calculated using a standard curve generated with specific standards ... vascular permeability-enhancing activities specific for endothelial cells It interacts with two receptor tyrosine kinases, VEGFR-1 (flt) and VEGFR-2 (flk) A defect in VEGF receptors could account ... 570 [26–1594]; P = 0.37) Concentrations of sVCAM-1 were not associated with SSc patient characteristics We could not discern an influence on sVCAM-1, VEGF, or sVEGFR-1 concentrations of inflammation...

Ngày tải lên: 09/08/2014, 01:23

... accession number Glucose transporter-1 Forward: CGTCTTCATCATCTTCACTG 148 [Genbank:NM_006516] β-Actin Forward: AACTACCTTCAACTCCAT 161 [Genbank:NM_001101] 155 [Genbank:NM_021130] Reverse: CTCCTCGGGTGTCTTATC ... CTCCTCGGGTGTCTTATC Reverse: TGATCTTGATCTTCATTGTG Cyclophilin A Forward: CAGTCCCAGGAAGTGTCAATG Reverse: CAGCGTCTCACTATGTTGCC Eugene, OR, USA) – a nonfluorescent probe that diffuses freely into cells, ... efficiency of the amplification reaction for each gene was calculated by running a standard curve of serially diluted cDNA sample, and the specificity of the amplification products was checked...

Ngày tải lên: 09/08/2014, 14:21

... content relative to nuclear DNA, the forward primer5'-GGCTCTGTGAGGGATATAAAGACA-3' and reverse primer 5'-CAAACCAC CCGAGCAACTAATCT-3' [complementary to sequences of chromosome (Chr1) genome loci ... strands of mtDNA and does not encode any functional proteins A mononucleotide repeat sequence (CCCC CCCTCCCCCC) between nucleotides 303 and 316-318 of mtDNA has been reported to be a hotspot of deletion ... (5'ACCAGTCTTGTAAACCGGAG-3') and H602 reverse primer (5'-GCTTTGAGGAGGTAAGCTAC-3') The products were purified with the PCR Product Pre-Sequencing Kit (GE Healthcare Life Sciences, Sweden) and sequenced...

Ngày tải lên: 11/08/2014, 07:21

... EBNA1 and Tat, expressed in C3 3a cells, were visualized as described in the materials and methods (C) Indirect immunofluorescence indicates EBNA1 is primarily nuclear, while Tat is nuclear and cytoplasmic ... indicated concentrations of paraquot, and (D) β-mercaptoethanol Transactivation is expressed as a percent of expression under control conditions Chelation of zinc, oxygen tension and oxidative stress ... formation of sulfenic (-SO), sulfinic (-SO2), and sulfonic acid (-SO3) [31] Chemical reductants such as beta-mercaptoethanol or dithiothreitol can reduce disulfide bonds, but have no effect on the other...

Ngày tải lên: 12/08/2014, 04:20

... CCC CAC CCC CAA TGT ATC CTC CGA TGC CTG CTT CAC TAC CTT AGC TTG CTG GTG AAA AGG AC TTA TAG TCA AGG GCA TAT CC TGG TGG TCC ACG AGA AAC GAG ATG CAA TGA CAC CAC AAG CCA AAC GAC T TGA GCC CAG CCC ... GCC TCG CTC CTC CGG AGG GAC GCC AAA CAG G GTC CCG GGT CAA CTC TTC GTG TGG AGA GCG TCA ACC GGG AGA TGT AGG TGT GCA GAT GCC GGT TCA GGT ACG CGG TCA GTC GGG TGC TC AAC GGC CTT TCC TGT TTT CTC CAG ... TGA CAA AAT G CTC GAG CCC GGA AAG GAC AGT T CTG GAG CGG CTG AAG GAC A AGC TCT GAG ATG CAC TGG ACA GCA ACC AGT TCG GGC ATC AG CGT TCA CCT CGC ACT TCT CAA AA TGT GGC ATC ATC GCA CGC TCT G GGG CCG...

Ngày tải lên: 13/08/2014, 13:20

... beta-boswellic acid, 11-keto-beta-boswellic acid, acetyl beta-boswellic acid, acetyl 11-keto-beta-boswellic acid, acetyl-alpha-boswellic acid, 3-oxo-tirucallic acid and 3-hydroxy-tirucallic acid Rats ... which is a chemotherapeutic drug used to treat cancer and can cause pulmonary fibrosis as a complication, has been used in research to induce pulmonary fibrosis in mice and rats and produce an oxidative ... Rosa C, Conte E, Crimi N, Cuzzocrea S, Vancheri C: 3.16,16-Dimethyl prostaglandin E2 efficacy on prevention and protection from bleomycininduced lung injury and fibrosis Am J Respir Cell Mol...

Ngày tải lên: 13/08/2014, 14:20

... Thorough peer review • No space constraints or color figure charges • Immediate publication on acceptance • Inclusion in PubMed, CAS, Scopus and Google Scholar • Research which is freely available for ... Sivarajah A, McDonald MC, Thiemermann C: The production of hydrogen sulfide limits myocardial ischemia and reperfusion injury and contributes to the cardioprotective effects of preconditioning ... Critical Care 2010, 14:R165 http://ccforum.com/content/14/5/R165 Page of 11 Figure Hemodynamic measurements Mean arterial blood pressure (MAP) and carotid blood flow (CBF) in hemorrhagic shock...

Ngày tải lên: 13/08/2014, 21:21

... 5¢-CTCCCTGGTCTCTCATCTGTCCTTCCCA CC-3¢; Myb3 Se, 5¢-CCTCCTGAGGCTTCCATCTGGCG GCCGCGG-3¢) Mutations were confirmed by nucleotide sequencing Transfection and luciferase activity assays Cells were cotransfected ... Clara, CA, USA) (primers: AP-1 Se, 5¢-CCGTCAGCGGT GACTTGGATTCACAGAGAC-3¢; FOXO Se, 5¢-CAAGT CACTAGGGTACCCACGCCGGGGTGG-3¢; Myb1 Se, 5¢-GACCAAGATGGTCCATCGGTGGGACGACAG-3¢; Myb2 Se, 5¢-CTCCCTGGTCTCTCATCTGTCCTTCCCA ... 5¢-CCAGACAATCGTCTCGCCCA-3¢; and Rv, 5¢-GGCTAGGTAACAGTTTAGCGAGGA-3¢) Rat genomic DNA extracted from rat primary hepatocytes was used as a positive control PCR products were analyzed by electrophoresis...

Ngày tải lên: 06/03/2014, 00:21

... experiments, and drafted the manuscript LTC, THT, YLC, SC, PLS, and CHY were responsible for the laboratory assay and troubleshooting KCC, SL, and HKY participated in refinement of experiment protocol and ... warranted to clarify the exact mechanisms involved Conclusions ADMSC markedly improved renal function after acute IR injury The key mechanisms underlying the positive therapeutic impact of ADMSC treatment ... antiinflammatory and anti -oxidative effects Third, the transplanted ADMSCs participated in angiogenesis early after IR injury Stem Cell Therapy Effectively Protected Renal Function from Acute Kidney Injury Complete...

Ngày tải lên: 18/06/2014, 19:20

... Deactivation of excited carbonyls and 1O2 produces photons (in the blue and red spectral regions, respectively) which can be recorded with a high-sensitivity, cooled CCD (charge coupled device) camera [34] ... (monomeric Lhcb and trimeric LHCII; B2 and B3 bands in Fig 10A, respectively) relative to the PSII reaction center (B5 band) The PSI-LHCI supercomplex (B6 band) was also reduced during photoacclimation ... type; PChlide and Chlide: protochlorophyllide and chlorophyllide; ROS: reactive oxygen species; HOTE: hydroxy octadecatrienoic acid; SOSG: singlet oxygen sensor green; NPQ: nonphotochemical quenching...

Ngày tải lên: 12/08/2014, 03:21

... sites including the predicted core promoter of dysbindin-1A was isolated using a forward CAGTCTCGAGAGGACTGGGGATGTCACTCA-3’) primer and reverse (5’primer (5’- GTACAAGCTTAACCCAGCCTTCTCCAAG-3’) ... α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid ARG Apoptosis response gene BLOC-1 Biogenesis of lysosome-related organelles complex BMD Becker muscular dystrophy Ca2+ Calcium CAT Catalase CCD Coiled coil domain Cdk1 Cyclin-dependent ... mitochondrion factors such as cytochrome -c and apoptotic-inducing factor (AIF) (5) triggering apoptosome complex formation and caspase-3 pathway activation; (6) leading to nuclear condensation and...

Ngày tải lên: 01/10/2015, 17:27

... fatty acids and vitamins, and other vitamins with the focus on the micro-algal sources of these compounds The Chlorophyceae class is rich in these nutraceuticals and genus Chlorella, Chlamydomonas, ... Chapter 22 Oxidative Therapy Against Cancer 497 Manuel de Miguel and Mario D Cordero Chapter 23 Monensin Induced Oxidative Stress Reduces Prostate Cancer Cell Migration and Cancer Stem Cell Population ... dismutase and perturbs free radical processes in the yeast Saccharomyces cerevisiae cells, Biochemical and Biophysical Research Communications, Vol 338, No 4, pp 1739-1744 Lushchak, V., Semchyshyn,...

Ngày tải lên: 12/02/2014, 16:20

... concentrations can be also a consequence of oxidative stress Thus, a vicious cycle can be formed Reducing carbohydrates as a factor of carbonyl /oxidative stress From the above sections it may be concluded ... Semchyshyn Section General Aspects of Oxidative Stress Chapter Interplay Between Oxidative and Carbonyl Stresses: Molecular Mechanisms, Biological Effects and Therapeutic Strategies of Protection ... loss of function and even viability can occur Recent studies indicate that in many cases increase in RCS steady-state concentrations is a consequence of oxidative stress, whereas increase in ROS...

Ngày tải lên: 12/02/2014, 16:20