examples of protein found in the human body

... explain the stabil- ity of the expressed proteins. In summary, the identification of three new and four previously reported mutations in the HMBS gene has increased our understanding of the molecular ... to the wild- type) and to a reduction of thermal stability. It is likely that the introduction of the positive charge of the lysine amino group attracts the carboxyl of Glu135 and brings the ... termi- nation. The mutated protein consists of 253 amino acids (361 in wild-type). The abrogated mutant lost the end of one b-sheet, one helix at the end of the central domain and the entire C-terminal...

Ngày tải lên: 23/03/2014, 04:21

... and so the choice of the constant level of Fru was not critical for determination of D K m (1.23 ± 0.15). The KIE data in Table 4 are instrumen- tal in delineating the kinetic mechanism of AfM1PDH and ... any other protein of the current PSLDR family, there is a good chance that compounds raised against AfM1PDH will not show substantial cross-reactivity with respect to binding of proteins of the human host. ... of magnitude below the apparent K m of 40 mm. A physiological role of AfM2DH in the utilization of intracellular Man-ol via reversal of path 2 in Scheme 1 is therefore proposed. In light of the...

Ngày tải lên: 14/03/2014, 23:20

... sequences contained in any of their strain members. For the 216-probe set, the minimum number of probes differentiating a DENV-1 strain from the all other strains belonging to one of the three other serotypes ... While these values contribute to the fit- ness of a set, the size of the set plays a much greater role in an effort to reduce the number of probes needed and thus the cost of the array. Therefore, ... 2. Number of unique human- blind sequences found in each of the 83 complete dengue virus strain genomes considering different sizes of n and number of changes away from the nearest human sequence....

Ngày tải lên: 23/03/2014, 11:20

Ngày tải lên: 12/08/2014, 11:22

Ngày tải lên: 14/08/2014, 08:20

Ngày tải lên: 14/08/2014, 14:21

Ngày tải lên: 14/08/2014, 14:21

Ngày tải lên: 22/12/2014, 20:09

Ngày tải lên: 18/12/2013, 21:45

... this is the first report of a post-translational modification of the ILT2 protein. The other member of the ILT family, LILRA4, has also been found nitrated within the domain Ig-like C2-type 4 in human ... an increase in binding capacity [25]. Identification of nitration site in the extracellular domain of ILT2 In the extracellular domain of ILT2, there are several Tyr residues that participate in ... band at 42 kDa. The data indicate the intensity of the HLA-G band related to the b-actin band and are representative of three different experiments. ILT2 nitration in the binding domain A. Dı ´ az-Lagares...

Ngày tải lên: 07/03/2014, 02:20

Ngày tải lên: 20/06/2014, 01:20

Ngày tải lên: 09/08/2014, 07:20

Ngày tải lên: 12/12/2013, 00:03

... FXa cofactor activity of the reaction intermediate, FVa int . This facilitated the determination of loss of FVa cofactor activity during the initial stage of inactivation and minimized the in uence ... the NMR/modeled structure of heparin (file 1hpn [35]). Amino acids known to be part of the heparin-binding site in these three crystallized protein heparin complexes were given as starting point ... inactivation of intact FV,but not FVa [22,23]. We performed a detailed kinetic analysis of the in uence of heparin on the inactivation of FVa by APC and found a specific heparin-mediated inhibition of the...

Ngày tải lên: 19/02/2014, 13:20

... localizes in the cytoplasm of human epithelial cells of the gastrointestinal tract and liver, two central organs in the regulation of iron metabolism [20,21]. After their synthesis in the endo- plasmic ... impact in protein integrity, as indicated by an increase in protein- bound acrolein adducts at the cell surface, which point to acrolein-adducts as a reliable marker of lipid peroxidation also in ... coordinated changes in the expression of the transferrin receptor and ferritin; (b) developed iron accumulation and (c) increased the prooxidant state and the level of oxidatively modified proteins....

Ngày tải lên: 19/02/2014, 16:20

... Numbering begins with the first nucleotide of the first exon ( up) and the first amino-acid residue of the mature M protein (down). Dashes indicate nucleotides identical with those of the M all ele. The ... 2002 Interallelic recombination at the a s1 -casein locus (Eur. J. Biochem. 269) 1303 skipping of three successive exons during the course of the processing of the primary transcripts [9]. At the ... substitution) occurring a t position 23 in exon 9 (TTGA instead of TCGA). To discriminate between the M and the F alleles, we took advantage of the presence o f an 11-bp insertion in intron 9 of the F allele,...

Ngày tải lên: 22/02/2014, 04:20

... hydration of the protein domain is the driving force of protein stabiliza- tion or folding [3,4]. Furthermore, the effect of osmolytes on the functional activity of an enzyme has also been investigated ... misfolded proteins [5–8] and remove protein aggregation [9–12]. Mechanisms of protein osmolyte interactions, the effect of osmolytes on protein stability, and how osmo- lytes correct protein misfolding ... DG D ° values in the presence of these osmolytes are given in Table 1. How- ever, DG D ° values of RNase-A in the presence of alanine, serine, lysine, b-alanine, taurine and dimethyl- glycine have...

Ngày tải lên: 07/03/2014, 00:20

Ngày tải lên: 07/03/2014, 02:20

... FEBS protein bound to the agarose. This binding was con- firmed by the observation that only a trace of the pro- tein was left in the unbound fraction. Pre-incubation of the recombinant protein ... against inactivation was observed and the initial GS activity was maintained (116.5 ± 7.3% of the initial activity). The protective effect was abolished when the recom- binant protein was heat-inactivated ... but the binding was not affected by preincubation of the protein with FP. These results also indicate that Pf1-Cys-Prx can bind to FP at pH 7.4, which is the pH of the parasite cytosol. The fact...

Ngày tải lên: 07/03/2014, 17:20