examples of protein found in the human body
Báo cáo khoa học: Acute intermittent porphyria – impact of mutations found in the hydroxymethylbilane synthase gene on biochemical and enzymatic protein properties pdf
Ngày tải lên :
23/03/2014, 04:21
... explain the stabil-
ity of the expressed proteins.
In summary, the identification of three new and four
previously reported mutations in the HMBS gene has
increased our understanding of the molecular ... to the wild-
type) and to a reduction of thermal stability. It is
likely that the introduction of the positive charge of
the lysine amino group attracts the carboxyl of
Glu135 and brings the ... termi-
nation. The mutated protein consists of 253 amino
acids (361 in wild-type). The abrogated mutant lost
the end of one b-sheet, one helix at the end of the
central domain and the entire C-terminal...
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Báo cáo khoa học: Enzymes of mannitol metabolism in the human pathogenic fungusAspergillus fumigatus– kinetic properties of mannitol-1-phosphate 5-dehydrogenase and mannitol 2-dehydrogenase, and their physiological implications pot
Ngày tải lên :
14/03/2014, 23:20
... and so the choice of the constant
level of Fru was not critical for determination of
D
K
m
(1.23 ± 0.15). The KIE data in Table 4 are instrumen-
tal in delineating the kinetic mechanism of AfM1PDH
and ... any
other protein of the current PSLDR family, there is
a good chance that compounds raised against
AfM1PDH will not show substantial cross-reactivity
with respect to binding of proteins of the human
host. ... of magnitude below
the apparent K
m
of 40 mm. A physiological role of
AfM2DH in the utilization of intracellular Man-ol via
reversal of path 2 in Scheme 1 is therefore proposed.
In light of the...
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Báo cáo khoa học: Human-blind probes and primers for dengue virus identification Exhaustive analysis of subsequences present in the human and 83 dengue genome sequences doc
Ngày tải lên :
23/03/2014, 11:20
... sequences contained
in any of their strain members. For the 216-probe set,
the minimum number of probes differentiating a
DENV-1 strain from the all other strains belonging to
one of the three other serotypes ... While these values contribute to the fit-
ness of a set, the size of the set plays a much greater role in
an effort to reduce the number of probes needed and thus
the cost of the array. Therefore, ... 2. Number of unique human- blind sequences found in each of
the 83 complete dengue virus strain genomes considering different
sizes of n and number of changes away from the nearest human
sequence....
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Báo cáo khoa học: Tyrosine nitration in the human leucocyte antigen-Gbinding domain of the Ig-like transcript 2 protein ppt
Ngày tải lên :
07/03/2014, 02:20
... this
is the first report of a post-translational modification
of the ILT2 protein. The other member of the ILT
family, LILRA4, has also been found nitrated within
the domain Ig-like C2-type 4 in human ... an increase in binding capacity
[25].
Identification of nitration site in the extracellular
domain of ILT2
In the extracellular domain of ILT2, there are several
Tyr residues that participate in ... band at 42 kDa. The data indicate the intensity
of the HLA-G band related to the b-actin band and are representative of three different experiments.
ILT2 nitration in the binding domain A. Dı
´
az-Lagares...
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Tài liệu Báo cáo khoa học: Altered inactivation pathway of factor Va by activated protein C in the presence of heparin doc
Ngày tải lên :
19/02/2014, 13:20
... FXa cofactor activity of the reaction
intermediate, FVa
int
. This facilitated the determination of
loss of FVa cofactor activity during the initial stage of
inactivation and minimized the in uence ... the NMR/modeled structure of
heparin (file 1hpn [35]).
Amino acids known to be part of the heparin-binding site
in these three crystallized protein heparin complexes were
given as starting point ... inactivation of intact FV,but not FVa [22,23].
We performed a detailed kinetic analysis of the in uence
of heparin on the inactivation of FVa by APC and found a
specific heparin-mediated inhibition of the...
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Tài liệu Báo cáo khoa học: Altered expression of CD1d molecules and lipid accumulation in the human hepatoma cell line HepG2 after iron loading pptx
Ngày tải lên :
19/02/2014, 16:20
... localizes in the cytoplasm of
human epithelial cells of the gastrointestinal tract and
liver, two central organs in the regulation of iron
metabolism [20,21]. After their synthesis in the endo-
plasmic ... impact in protein integrity, as indicated
by an increase in protein- bound acrolein adducts at
the cell surface, which point to acrolein-adducts as a
reliable marker of lipid peroxidation also in ... coordinated
changes in the expression of the transferrin receptor
and ferritin; (b) developed iron accumulation and
(c) increased the prooxidant state and the level of
oxidatively modified proteins....
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Tài liệu Báo cáo Y học: Interallelic recombination is probably responsible for the occurrence of a new as1-casein variant found in the goat species potx
Ngày tải lên :
22/02/2014, 04:20
... Numbering begins with the first nucleotide of the first exon ( up) and the first amino-acid residue of the mature M protein (down).
Dashes indicate nucleotides identical with those of the M all ele. The ... 2002 Interallelic recombination at the a
s1
-casein locus (Eur. J. Biochem. 269) 1303
skipping of three successive exons during the course of the
processing of the primary transcripts [9]. At the ... substitution) occurring a t position 23 in exon 9
(TTGA instead of TCGA).
To discriminate between the M and the F alleles, we took
advantage of the presence o f an 11-bp insertion in intron 9
of the F allele,...
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Báo cáo khoa học: Relationship between functional activity and protein stability in the presence of all classes of stabilizing osmolytes ppt
Ngày tải lên :
07/03/2014, 00:20
... hydration of the
protein domain is the driving force of protein stabiliza-
tion or folding [3,4]. Furthermore, the effect of
osmolytes on the functional activity of an enzyme has
also been investigated ... misfolded
proteins [5–8] and remove protein aggregation [9–12].
Mechanisms of protein osmolyte interactions, the
effect of osmolytes on protein stability, and how osmo-
lytes correct protein misfolding ... DG
D
° values in the
presence of these osmolytes are given in Table 1. How-
ever, DG
D
° values of RNase-A in the presence of
alanine, serine, lysine, b-alanine, taurine and dimethyl-
glycine have...
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Báo cáo khoa học: Roles of 1-Cys peroxiredoxin in haem detoxification in the human malaria parasite Plasmodium falciparum potx
Ngày tải lên :
07/03/2014, 17:20
... FEBS
protein bound to the agarose. This binding was con-
firmed by the observation that only a trace of the pro-
tein was left in the unbound fraction. Pre-incubation of
the recombinant protein ... against
inactivation was observed and the initial GS activity
was maintained (116.5 ± 7.3% of the initial activity).
The protective effect was abolished when the recom-
binant protein was heat-inactivated ... but the binding
was not affected by preincubation of the protein with
FP. These results also indicate that Pf1-Cys-Prx can
bind to FP at pH 7.4, which is the pH of the parasite
cytosol. The fact...
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