evidence for posttranscriptional site of ethanol action

... RT-PCR4analysis of virion RNAs RT-PCR analysis of virion RNAs A Plasmid structures of retroviral leader regions L1 and L2, primers used for PCR amplification; sizes of DNA fragments and positions of EcoRI sites ... the main reason for the preferential formation of homodiploid genomes in Mo-MuLV In spite of substantial differences in the methods, the estimations of the efficiency of homodimer formation were ... populations of RNA molecules left in a monomeric form and/or unstable homodimers The diploidy of retroviral genome supposes that two molecules of RNA could be necessary for replication of virus...

Ngày tải lên: 13/08/2014, 13:20

... the G/E fraction Assessment of DPP IV distribution in our hepatic fractions, using anti-DPP IV (26C), showed that  80% of the amount of DPP IV detected was located in the G ⁄ E fraction No DPP ... 100 000 g for h and the supernatant was collected Protein content of fractions was determined by a modification of the Bradford method using BSA as a standard Statistical analysis was performed with ... presence of an associated band around 56 kDa, presumably c-Src itself or a putative substrate (Fig 3B) Localization of c-Src in G/E fractions Fig Distribution of DPP IV in hepatic subcellular fractions...

Ngày tải lên: 19/02/2014, 07:20

... of calcium is important for the optimal activity of BsArb43B Furthermore, to determine whether the role of the calcium is structural, thermal shift assays were performed to determine the Tm of ... different colours: blue for BsArb43B, magenta for BsArb43A, green for ABNTS, dark grey for AbnB, red for exo-Abn towards the solvent region, and presumably the other residues of the arabinohexose ... lack of accumulation in vivo indicates poor stability, and suggests that the presence of the C-terminal domain is crucial for the acquisition of the correct enzyme fold Analysis of the interactions...

Ngày tải lên: 15/03/2014, 23:20

... boiled at 95 °C for The sample for electrophoresis analysis was obtained by collecting the supernatant ( 30 lL) after centrifugation at 12 000 g for 20 s and addition of 10 lL of 75% (w/v) glycerol ... suggested, they represent ferritin and a product of degradation of the same protein [14] The same crossreactivity was also detected for a protein of twofold purified pea stem mitochondria (result not ... presequences of the chloroplastic protein transit peptide [28], similarly to what reported for pea ferritin [29,30] On the other hand, the analysis of the presequence of AtFer4 reveals a high score for...

Ngày tải lên: 16/03/2014, 18:20

... second-order decays of TyrOÆ and TrpÆ The protein was present at 0.14 mmolÆL)1 (2 mgÆmL)1), except when we determined the concentration dependence of the rate constant for reaction (1) For this experiment ... gradient of 35–60% (trifluoroacetic acid 0.1%/acetonitrile 70%) in trifluoroacetic acid over 30 (at a flow rate of 1.5 mLÆmin)1) was used Detection was at 214 nm Evaluation of the fraction of dimerized ... achieved by calculation of the portion of the total area under the curve occupied by the dimers peak The obtained value was divided by two MALDI-TOF MS was performed at The Institute of Nuclear Physics,...

Ngày tải lên: 17/03/2014, 10:20

... staining of 28S rRNA is shown to allow comparison of RNA amounts loaded (C) Quantification of results for BBB-IL-8972)1310 mRNA from an experiment performed as in (B), but with different doses of UV ... before the assay of RNA stability Half-lives for BBB-IL-8972)1310 were quantified as described in Materials and Methods 5834 F Bollig et al (Eur J Biochem 269) Ó FEBS 2002 1) Formation of two of ... consisting of the ARE of GM-CSF or the AU-rich region of the IL-8 3¢-UTR (nucleotides 972–1310) Where indicated, antibodies specific for HuR (0.75 lg), AUF1 (1 lL of a : 10 dilution of serum) or...

Ngày tải lên: 31/03/2014, 08:20

... results of wider study of the giving of law and covenant in Sinai, of the results of modern study of the ancient use of portable structures (‘‘tabernacles’’) for worship and cult, and of the occurrence ... prior permission of Oxford University Press Library of Congress Cataloging-in-Publication Data Hoffmeier, James Karl, 1951– Ancient Israel in Sinai : the evidence for the authenticity of the wilderness ... Journal of Near Eastern Studies Jewish Publication Society Jewish Quarterly Review Journal for the Study of the Old Testament Journal of Semitic Studies Journal of the Society for the Study of Egyptian...

Ngày tải lên: 11/06/2014, 01:36

... TreePuzzle for a smaller data set Phylogenetic tree created with TreePuzzle for a smaller data set The tree is based on the nt 37–1232 of the S segment sequences Page of (page number not for citation ... likelihood method for reconstructing tree topologies Mol Biol Evol 1996, 13:964-969 Posada D, Crandall KA: MODELTEST: testing the model of DNA substitution Bioninformatics 1998, 14:817-818 Swofford DL: ... outgroup In the case of maximum likelihood trees, the use of additional hantavirus sequences as outgroup resulted in a lower bootstrap support for SAAV In fact, a 100% support for SAAV monophyly...

Ngày tải lên: 19/06/2014, 08:20

... TreePuzzle for a smaller data set Phylogenetic tree created with TreePuzzle for a smaller data set The tree is based on the nt 37–1232 of the S segment sequences Page of (page number not for citation ... likelihood method for reconstructing tree topologies Mol Biol Evol 1996, 13:964-969 Posada D, Crandall KA: MODELTEST: testing the model of DNA substitution Bioninformatics 1998, 14:817-818 Swofford DL: ... outgroup In the case of maximum likelihood trees, the use of additional hantavirus sequences as outgroup resulted in a lower bootstrap support for SAAV In fact, a 100% support for SAAV monophyly...

Ngày tải lên: 20/06/2014, 04:20

... stage of infection These empirical estimates for coital frequency were then used in Goodreau et al [27] for the three of their four transmission models that were built off of published estimates for ... dependence that is of core interest, not merely something to control for For their series of concurrency modelling papers, Morris and Kretzschmar developed an elaborate set of computer code to ... biological data in all of the ways that Sawers and Stillwaggon outline as necessary for a realistic model, although all incorporated some forms of data Nevertheless, this line of work confirmed the...

Ngày tải lên: 20/06/2014, 08:20

... pattern of gene expression that mirrors this Journal of Biology 2002, 1:5 http://jbiol.com/content/1/1/5 Journal of Biology 2002, Table The number of groups of genes, and total numbers of genes ... so we used a window size of ten for the remainder of our analysis There are no qualitative differences in the nature of groups identified by larger window sizes Many of the ten-gene groups that ... expression was calculated for genes in a sliding window across the genome, and this process was repeated for windows of different sizes The net number of genes (that is, the number of genes in groups...

Ngày tải lên: 06/08/2014, 18:20

... occurred in the leaf xylem of the drought trees in the absence of regulation We can thus predict that because of these tensions and because of runaway embolism, the degree of embolism would have ... revealed the role of specific hormones (ABA) in the control of g during drought Roots in s the dry zones of the soil are producing ABA that provoke stomatal closure An argument in favor of hormonal ... computed that for the driest trees, the dif- ference between dF and dF cavitation midday represented less that a few percent of the midday dF of the control trees It can be noted that for some droughted...

Ngày tải lên: 08/08/2014, 18:21

... the nucleoplasm and therefore allow forces produced within the cytoplasm to be transmitted to the nuclear periphery Evidence for the expression and NE localization of Page of 22 plant SUN-domain ... first description of relative mRNA expression levels of all members of a SUN gene family in any plant species and may therefore prove useful to investigators of the functions of plant SUN-domain ... typical of the entire class of PM3 proteins Localization of a Maize PM3-type Protein To test for the presence and localization of ZmSUN3/4 proteins in planta, we developed peptide antibodies for...

Ngày tải lên: 11/08/2014, 11:21

... 50°C for min, 95°C for min, followed by 35 cycles of incubation at 95°C for 20 s, 58°C for 20 s, 72°C for 20 s, and a final extension step of 10 at 72°C In order to identify possible effects of ... organization of the gene The cycling parameters were: 50°C for min, 95°C for min, followed by 35 cycles of incubation at 95°C for 20 s, 58°C for 20 s, 72°C for 25 s, and a final extension step of 10 ... region of the gene The role, if any, of these SNPs in binding sites of regulatory elements remains to be studied Page of 16 VIGS of CaOvate in cv “Round” In order to obtain further evidence for...

Ngày tải lên: 11/08/2014, 11:21

... knowledge of the possible functions of the genes in the region confirmed the former hypothesis of Costantini et al [4] that VvAGL11 is the best positional candidate gene for the control of seed ... positional evidence for VvAGL11 as being the major gene responsible for seedlessness [4] Here, we provide genetic and transcriptional support for this hypothesis and discuss its potential for molecular-assisted ... segregating population of only ~ 125 phenotyped individuals As the development of a wellbalanced population in terms of phenotypic classes for seedlessness requires a step of in vitro embryo rescue...

Ngày tải lên: 11/08/2014, 11:21

... irritable only) of seven symptoms during the period of mood disturbance Also recorded was the onset of first episode, the number of episodes, offset of last episode, and total duration of illness ... subcommittee for human subjects of our institution All subjects' parents or guardians signed written informed consent forms and children older than years of age signed written assent forms Potential ... for multiple testing was implemented based on the number of SNPs analyzed per gene The critical P-value for a positive association was therefore 1.6 × 10-4 for SNPs from RORA and 1.2 × 10-3 for...

Ngày tải lên: 11/08/2014, 17:20

... definition of anti-HCV positivity (type of biochemical test used); location (city, country, number and type of treatment settings); years of recruitment (and total duration of recruitment); number of ... edited version of the World Health Organization Health Evidence Network synthesis of the evidence base pertaining to effectiveness of interventions to reduce the incidence and prevalence of hepatitis ... reported in the form of a narrative systematic review Such a narrative format has been described as appropriate in reporting the results of observational studies identified through a process of systematic...

Ngày tải lên: 11/08/2014, 20:20

... understanding of the role of circulating and tissue sources of ACE2, particularly in various disease states Increased circulating levels of ACE2 may reflect a compensatory mechanism to alter the balance of ... identification of endogenous ACE2 inhibitors is important in lieu of optimizing the therapeutic benefits following administration of recombinant soluble ACE2, as recently demonstrated in models of diabetic ... genetic expression of ACE2 in pulmonary hypertension [10] Page of Although the ongoing study of the renin–angiotensin system has now surpassed the century mark, the characterization of this system...

Ngày tải lên: 12/08/2014, 14:22

... number in the category; E for expected gene number in the category; R for ratio of enrichment for the category; and P for p value calculated from the statistical test given for the categories with ... GOTree In GOTree, O stands for observed gene number in the category; E for expected gene number in the category; R for ratio of enrichment for the category; and P for p value calculated from ... analysis: A knowledge-based approach for interpreting genome-wide expression profiles Proceedings of the National Academy of Sciences of the United States of America 2005, 102:15545-15550 25 Cicala...

Ngày tải lên: 13/08/2014, 01:20

... function, which, for the particular event of interest, is a function of the hazards of all the competing events and not solely of the hazard of the event to which it refers Hypothesis tests for the cumulative ... 14:R134 http://ccforum.com/content/14/4/R134 site The time span of the studies suggested that definitions for secondary infections would be subject to revision; for example, the use of quantitative ... intersection of the (meta)regression line with the line of null effect (’cross-over’ point) occurred for age at 62 years and for control-arm mortality at 44% Complications of therapy The complications of...

Ngày tải lên: 13/08/2014, 21:21