evaluating the gene expression profile of ho

... computing the log2 of the ratio between the gene expression level in TEV-infected plants and control plants (mock inoculated) and normalized by the number of genes in the set To minimize the number of ... significance of these scores, sets of genes were randomly selected and their scores computed For GO groups, the sets contained 50 genes, and for the KEGG pathways, the set contained an average of eight genes ... miRNA-regulated genes Conclusion The data presented in this study demonstrates the varied effects at the transcriptomic level of TEV infection on a susceptible host The observed changes in gene expression of...

Ngày tải lên: 20/06/2014, 01:20

... the target organ, the joint Bhsp65-induced gene expression profile of LEW rats in the preclinical phase of AA The analysis of Bhsp65-induced gene expression profile of rats in the Inc phase of ... the Inc phase of arthritis instead of the Pk phase of the disease In fact, most of the genes showed significantly reduced expression at the Pk and the Rec phase compared to the Inc phase As the ... served as the baseline non-arthritic controls The choice of ex vivo testing of LNC was made to obtain a snapshot of the unperturbed gene expression profiles closely depicting the in vivo gene expression...

Ngày tải lên: 12/08/2014, 18:20

... to the variable effects of prostratin in the absence of Tat when viral gene expression is low The overall outcome of viral gene expression observed in this study may represents the net result of ... CD4+ T cells The size of individual nodes is indicative of the numbers of genes involved in the category and the color represents the level of significance, with orange indicating the highest ... into the hyperphosphorylated form of CTD (CTDo) were quantified using the Storm 860 PhosphorImager system (Molecular Dynamics) Northern blots Total RNA from cell extracts with the same amount of...

Ngày tải lên: 13/08/2014, 09:20

... [43-45] Expression of viral RSS in transgenic plants have been shown either to decrease the amount of miRNAs, or to reduce the activity of the silencing processes, which should lead to increase of the ... thioreduction of these proteins in cytoplasm could easily impair photosynthesis, and thereby lead to sugar starvation and further on, to altered regulation of the metabolic stress-related genes The general ... of the main components in cell wall against invading pathogens Endo-polygalacturonase (PG), one of the enzymes secreted at the early stages of infection, depolymerizes the homogalacturonan, the...

Ngày tải lên: 11/08/2014, 11:22

... not shown) Expression of these genes was no more detectable days after high-dose Xray administration (Figure 6a-k) On the contrary, the D japonica homolog of AHNAK was found at the level of the ... In all cases the results obtained were consistent with the transcriptional profile detected by microarray analysis (Figure 5) The expression of some of these genes was analyzed by wholemount in ... [7] These cells are scattered throughout the parenchyma with the exception of the anterior end of the cephalic region and the pharynx, and accumulate preferentially in the dorsal region, along the...

Ngày tải lên: 14/08/2014, 20:22

... imply the involvement of the O-glycosylation of Sp1 in the induction of SREBP-1a transcription by glutamine The gene expression of SREBP-1c, which represented less than 20% of the SREBP-1a gene expression ... Induction of SREBP gene expression is not the initial trigger for the glutamine effects If induction of the SREBP gene expression serves as the initial trigger for the glutamine effects, the gene expression ... affects the expression of the genes involved in cholesterol homeostasis The results clearly demonstrate that glutamine stimulates SREBP processing and the gene expression of SREBP targets The same...

Ngày tải lên: 28/03/2014, 22:21

... to make cDNA and then the ratio of the Housekeeper gene to the gene of interest was used Because the housekeeper gene is not affected by the treatment, the Ct and the efficiency of amplification ... chosen [13,14] Table shows the abbreviation, major activity, and the source of expression of the investigated mRNA transcripts A housekeeper gene was used to correct for the absolute amounts of ... Plasma levels of inflammatory mediators in sepsis reflect the overflow of these mediators into the bloodstream and may give limited insight into the actual activation of the leucocytes and the innate...

Ngày tải lên: 20/06/2014, 23:20

... Results Gene expression profile in joints at onset of arthritis To define the gene expression profile at early stages of CIA, we used the murine Affymetrix oligonucleotide microarray Page 871 of 876 ... Summary of differentially expressed genes at the early stage of arthritis genes at the early stage of arthritis Of the approximately 22,000 genes on the Affymetrix chip MOE430a, 655 genes were ... addressing the expression profile of joints in CIA mice at the peak of the disease [13], we intentionally focused on an early stage in the disease, in which the animals were free of clinical...

Ngày tải lên: 09/08/2014, 06:23

... A total of 63.7% of highly expressed genes and 70.6% of low expressed genes of trichomes were annotated http:// amigo.geneontology.org The predicted gene sets for the high and the low expression ... microarrays of these ESTs were used to analyze the gene expression of trichome By probing the cDNA microarrays with RNA samples from trichomes and leaves-minus-trichomes, 207 upregulated genes in trichomes ... was a homolog of the cytochrome P450 gene, CYP71D16, involved in diterpenoid biosynthesis of tobacco trichomes [15] Both the two genes were expressed exclusively in trichomes No Table The 20 genes...

Ngày tải lên: 11/08/2014, 11:20

... classifications for the majority of apple genes on the array Table shows the number of apple genes, the number of genes with Arabidopsis matches, the number of matches to unique Arabidopsis genes and the number ... EST on the array is shown for the best apple gene match to the Arabidopsis gene b The significance of the BLAST comparison between the Arabidopsis gene and the best apple gene c The degree of correspondence ... 2722 1128 1916 300 885 1552 The table shows the number of genes on the whole array and within the clusters as well as the number of Arabidopsis homologues and the number of MIPS function classifications...

Ngày tải lên: 12/08/2014, 05:20

... (Table 1) provided the opportunity to establish whether each region of the CNS exhibits a unique pattern of gene expression Gene expression profiles were generated for each of the five brain regions ... change (Figure 2a) The expression level of about 90% of the genes on the array was statistically unaffected by age For most brain regions, more than half of the genes whose expression level changed ... Aging undertook the AGEMAP (Atlas of Gene Expression in Mouse Aging Project), a comprehensive analysis of the effects of aging, CR and gender on gene expression in tissues throughout the body Here...

Ngày tải lên: 14/08/2014, 08:20

... the genetic and phenotypic heterogeneity of tumors Moreover, individual genes are identified rather than sets of genes that share a biological function Here we report the first expression profile ... against the consensus sequence of the known transcripts for the target gene Microarrays carry multiple probes against the same gene distributed throughout the length of the transcript, some of which ... in the lists of genes found to be differentially expressed It was shown that co-culture of hepatic stellate cells with Kupffer cells in vitro (acting as feeders) shifted the gene expression profile...

Ngày tải lên: 14/08/2014, 08:21

... 535/587 nm) The assay detects total 24 cholesterol (cholesterol and cholesteryl esters) in the presence of cholesterol esterase or free cholesterol in the absence of cholesterol esterase in the reaction ... is often regarded as a key early event in the development of atherosclerosis thus further study of these genes may lead to a better understanding on the role of hypertension in stroke List of ... information arriving from the thalamus to the forebrain 1.5 Animal Model of Hypertension Much of the understanding of the molecular mechanisms involved in the pathophysiology of the cardiovascular system...

Ngày tải lên: 02/10/2015, 17:13

... http://www.geneontology.org/) of the differentially expressed genes are shown in table Figure Comparison of the expression level of the reference genes The main modified genes (grey in table 2) and the ... expression of BSG gene It is coincidence that the detected genes, the PKN1 and BSG gene cluster on the adjacent chromosomal location: The gene expression data show that the short arm of the chromosome ... alteration of the cellular phenotype as demonstrated using the expression of the GFP reporter gene We show here that not only the experimental design of active cargos but also the choice of the TPs...

Ngày tải lên: 03/11/2012, 11:44

... criteria of good tests and theoretical ideas on test reliability and validity as well as achievement tests Chapter 3- The study is the main part of the thesis showing the context of the study and the ... reliability estimated by the split half method; r : the correlation of the A, B scores from one half of the test with those from the other half) Rational equivalence is another method which provides ... the high group, Lc: number of correct responses in the low group) The range of discriminability is from to The greater the D index is, the better the discriminability is The item properties of...

Ngày tải lên: 07/11/2012, 15:05

... measure of gene behavior emulates at the molecular level the loss of homeostasis characteristic of disease pathogenesis The method identified a significant number of genes relevant to the pathophysiology ... deviation of the gene expression ordinates from a regression line calculated against of the averaged profile for gene expressions in all samples of the control group The majority of these deviations ... groups are then scaled to the averaged profile of the control group The two main sources of heterogeneity in gene expression variations are the ‘additive component’, prominent at low expression...

Ngày tải lên: 09/08/2014, 01:23

... specific gene- expression profile that should explain the molecular nature of their etiological differences Candidate gene profiles in each group Using the SAM software, we examined 1,035 genes to ... 1) Gene- expression profiles and clustering As shown in Fig 3, 18 samples from 16 cases were clustered into two major groups based on their gene- expression profiles The dendrogram shown at the ... On the basis of these findings, we speculate that the two groups may reflect differences in the pathogenesis of synovitis The different expression profiles of several candidate genes for RA reported...

Ngày tải lên: 09/08/2014, 06:23

... samples were included in the analysis The top 20 terms for genes down-regulated after bleomycin sulphate are shown a The number of genes involved in the term are shown b The modified Fisher Exact ... biogenesis 98 1.81E-05 All 16 fibroblast samples were included in the analysis The top 20 terms for genes up-regulated after bleomycin sulphate are shown a The number of genes involved in the ... One of the main limitations of the study was the sample size This and the related issue of inter-sample variation may have contributed to the negative findings An additional possible source of...

Ngày tải lên: 09/08/2014, 09:20

... analysis of expressed genes were performed by using the web-based tools Onto-Express (OE) and Pathway-Express (PE) The aim of this study was to assess the relation of the gene expression profile ... dendogram to the left of the heat map shows clustering of the genes Accession number, gene symbol, gene description and fold change were added Colour boxes indicate the biological process of each gene ... left of the heat map shows clustering of the genes Accession number, gene symbol, gene description and fold change were added Colour boxes indicate the biological process of each gene Page of (page...

Ngày tải lên: 09/08/2014, 09:22

... 'snapshot' of the gene expression in OA bone, with contributions from all of the different cells in the bone microenvironment Although the specific cell type(s) contributing to the altered gene expression ... potential chondrogenic functions Altered expression of many of these genes is consistent with the proposal that similar altered expression of these genes in osteoblasts in the subchondral bone ... Normalized gene expression values for each gene based on cycle threshold (CT) values for each of the genes and the housekeeping gene GAPDH were generated Mean ± standard deviation (SD) values were generated...

Ngày tải lên: 09/08/2014, 10:21